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Enteric coated beta-mannase

A technology of mannanase and enteric coating, which is applied in the forming or processing of animal feed, animal feed, additional food elements, etc., and can solve the problem of undetectable enzyme activity

Inactive Publication Date: 2010-12-22
BEIJING ZHONGSHANG WEIZHENG TECH DETECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The authorized invention patent: a β-mannanase and its coding gene and application (authorization number CN100410380) discloses that the optimal pH of the prepared recombinant β-mannanase is 6.4, and at pH4.6- In the range of 9.0, the enzyme activity remains more than 50%, and the enzyme activity is basically undetectable below pH 3.0

Method used

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  • Enteric coated beta-mannase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Enteric coating treatment of embodiment 1.β-mannanase

[0019] according to figure 1 Coated β-mannanase granules are prepared in the following steps, the first step being to form a compressible mixture which is then fed to a granulator / extruder to produce a compressed extrudate , after recovering the particles, they are dried in a fluidized bed.

[0020] 45% microcrystalline cellulose is mixed with refined β-mannanase, and the enzyme specific activity of β-mannanase is 15000U / mg. First rough mix in a closed weighing container, then place it in a mixer, slowly add cold water under mixing conditions to wet the homogeneous mixture to a water content of about 30%, and mix to form a wet mass.

[0021] The wet mass was then fed into the hopper of a granulator and granulated / extruded through a domed die at a screw speed of 50 rpm. The extrudate was placed in a spheronizer and spheronized for three minutes or until uniform granules were formed, the resulting wet granules wer...

Embodiment 2

[0024] The coated granules of the present invention were prepared according to the same process as in Example 1, except that the composition of the coating material was different.

[0025] Fluid bed dried β-mannanase granules 2500g

[0026] Coating material: liquid shellac 300ml

[0027] Corn Gluten Extract 250g

[0028] Glycerin 50ml

[0029] 90% ethanol 200ml

Embodiment 3

[0031] The coated granules of the present invention were prepared according to the same process as in Example 1, except that the composition of the coating material was different.

[0032] Fluid bed dried β-mannanase granules 2500g

[0033] Coating material: corn protein extract 300g

[0034] Glycerin 50ml

[0035] 90% ethanol 550ml

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Abstract

The invention relates to an enteric coated granule of beta-mannase, a preparation method and application thereof in the feed industry. The invention improves the stability of the beta-mannase through carrying out enteric coated treatment on the beta-mannase, reduces the influence of physiological environment of animals on the enzyme activity and is beneficial to improving the application effect of the beta-mannase in feeds.

Description

technical field [0001] The invention relates to an enteric coated granule with better stability of β-mannanase. In the description of the present invention, β-mannanase (EC3.2.1.78) is a kind of hemicellulolytic enzyme, which degrades 1,3-1,4 glycosidic bonds in an endo-cutting manner, and the non-reducing end of the degradation product is Mannose, its substrates include glucomannan, galactomannan and mannan. The purpose of the coating is to improve the stability of the β-mannanase, which is beneficial to the decomposition and nutrient absorption of main anti-nutritional factors such as mannan substances in the feed. Background technique [0002] β-mannanase (β-1,4-mannan mannohydrolase, referred to as β-D-mannanase or β-1,4-D-mannanase, EC3.2.1.78) is a class of Endohydrolase of mannan oligosaccharides and mannan polysaccharides (including mannan, galactomannan, glucomannan, etc.) containing β-1,4-D-mannosidic bonds. β-mannanase belongs to hemicellulase, which is a broad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165A23K20/189A23K40/30
Inventor 刘洁平石慧
Owner BEIJING ZHONGSHANG WEIZHENG TECH DETECTION
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