94 results about "Brassinosteroid" patented technology

The present invention provides a BZR1 gene and an altered gene, bzr1-D, involved in the brassinosteroid response pathway. The expression of BZR1 genes in transgenic plants causes such plants to become significantly larger and more robust than their wild-type counterparts, thus increasing plant yields.

The present invention relates to novel, polynucleotides isolated from dwarf plants. The dwf4 polynucleotides that encode all, or a portion of, a DWF4 polypeptide, a cytochrome P450 enzyme that mediates multiple steps in synthesis of brassinosteroids. The present invention also relates to isolated polynucleotides that encode regulatory regions of dwf4. Uses of the dwf4 polypeptides and polynucleotides are also disclosed.

A green efficient seed dressing agent is provided by the present invention, and belongs to the technical field of pesticide. The green efficient seed dressing agent consists of pure natural plant extracts such as brassinosteroid, algaeinc and tea saponin, and bio-functional bacteria such as Bacillus subtilis, actinomycetes, Beauveria bassina, trichoderma and Bacillus thuringiensis. The seed dressing agent is green and environmentally friendly, and has good effects on improving the germination rate and emergence rate of seeds, and on insect killing and sterilization.

The invention discloses a sample pretreatment method of the endogenous brassinosteroids in a plant sample. The method comprises the following steps that: firstly, the endogenous brassinosteroids are extracted by a solvent; and then the impurities of the solution obtained in the previous step are further removed with boron-affinity material as an extraction medium, so that the sample pretreatment of the endogenous brassinosteroids is realized. The method has the advantages of simplicity, rapidness, solvent conservation and simplicity in operation. Besides, according to the method, high selectivity is shown in the aspect of removing endogenous impurities of plant extracting solution and enrichment and purification of low-content endogenous brassinosteroids can be realized.

The invention belongs to the field of plant gene engineering and particularly relates to separated clone, function verification and application of a gene PM1 (Panicle Morphogenesis 1) for controlling the morphogenesis of paddy rice spike. External application of brassin steroid hormone (BR), PM1 gene expression analysis, construction of complementary carrier genetic transformation and other experiments testify that the cloned PM1 gene has the function of regulating and controlling the form of paddy rice spike. The PM1 gene and the coded protein can be used for genetic improvement of the paddy rice spike forms and grain shapes, and has an important production application value.

The invention relates to the field of skin-care products, and discloses a skin-care product composition for repairing damaged cells and sensitive skin and a preparation method thereof. The skin-care product composition comprises the following raw materials in percentage by weight: 88-92% of an emollient, 6-10% of a skin conditioner, and 1-2% of a thickening agent, wherein the emollient comprises at least one of the following components: squalene, butyrospermum parkii fruit fat, and triethylhexanoin; the skin conditioner comprises a skin conditioner 1 and a skin conditioner 2; the skin conditioner 1 comprises at least one of brassinosteroid, cetostearyl alcohol, a radix arnebiae extract, allantoin, lavender oil, rose oil, bisabolol, a butyrospermum parkii fruit fat extract, spent grain wax,argan oil, a ginger extract, glycerinum, a flos chamomillae recutitae extract, and hydroxyphenylpropionamide benzoic acid; the skin conditioner 2 comprises at least one of butanediol, 1,2-pentanediol, a red-knees herb extract, and xanthan gum; and the thickening agent comprises at least one of the following components: polyethylene, vinyl polydimethyl siloxane / polymethylsiloxane silsesquioxane cross-linked polymer.

The invention relates to a method for conferring tolerance to abiotic stress to plants or plant cells. This is done by introducing a gene coding for an RKS protein, especially a gene coding for an RKS subgroup II protein, more specifically RKS1, RKS4 or truncated RKS4, or a gene from RKS subgroup III, more preferably RKS12. The effect of overexpression of the RKS gene may be enhanced by additionally treating the plant with a brassinosteroid compound.

The invention discloses a method of increasing growth of lateral roots of Malus hupehensis. The method of increasing growth of lateral roots of Malus hupehensis includes the steps: after storing seeds of Malus hupehensis in sand for one month, planting the seeds of Malus hupehensis in a nutrition pot; selecting 8-10 leaves and 60-days old and insect-disease-free seedlings of Malus hupehensis, and taking the seedlings of Malus hupehensis away from the nutrition pot, rinsing the matrix by means of clean water, and transplanting the seedlings of Malus hupehensis to a greenhouse; transplanting the seedlings of Malus hupehensis to 1 / 2Hoagland water planting nutrient solution for seedling recovering for half a month, and exchanging the nutrient solution once a week; and performing BR (brassinosteroid) processing, wherein the BR processing concentration is 0.5mg / l, normally managing after one week of processing, exchanging the nutrient solution once a week, and cultivating for one month. For the method of increasing growth of lateral roots of Malus hupehensis, the BR processing increases the number of roots, the length of roots, the surface area of roots, the volume of roots, the height of plants, and the thickness of stems of the seedlings of Malus hupehensis. In a word, the RB processing is conductive to growth of lateral roots, and especially increases growth of fine roots. The method of increasing growth of lateral roots of Malus hupehensis can provide certain theoretical basis for growth of lateral roots of apple, and has important meaning for guiding practical production.

The present invention provides a rapid and efficient detection method of brassinosteroid. The rapid and efficient detection method comprises: adopting fresh grape fruits as a material, rapidly grinding into powder with liquid nitrogen, adding 80-95% methanol, isotope internal standard 2H3 brassinosteroid and 2H3 brassin sterone, carrying out low temperature ultrasonic extraction, standing for 2-5h, filtering, taking the filtration residue, adding 80-95% methanol, leaching for 1-2 h, filtering, merging the two filtrates, carrying out physical adsorption to remove the water, sequentially loading onto a C18 column, a sephadex LH-20 column and a silica column, eluting with methanol to obtain an enriched sample liquid, adding phenylboronic acid, carrying out a derivatization reaction, cooling, concentrating to achieve a dry state, redissolving with acetonitrile so as to be used for liquid chromatography determination, selecting acetonitrile and water as the mobile phase, and collecting the target peak, wherein a volume ratio of the acetonitrile to water is (6:4)-(9:1), and the elution way is isocratic elution. The method of the present invention has characteristics of rapidness, efficiency, stable baseline and good brassinosteroid separation.

The invention relates to a Chinese cabbage AP2 / ERF transcription factor gene and applications thereof and belongs to the technical field of plant genetic engineering. A complementary deoxyribonucleic acid (cDNA) sequence of the gene is represented as SEQIDNo.1. The Chinese cabbage AP2 / ERF transcription factor gene is applied in nightshade tobacco bacterial wilt-resisting genetic engineering. When transgenic tobacco is compared with wild tobacco, the overexpression of a Chinese cabbage brassinosteroid ethylene responsive factor 11 (BrERF11) gene in the tobacco can significantly improve the bacterial wilt-resisting ability of the transgenic tobacco, so that the Chinese cabbage AP2 / ERF transcription factor gene has an important application value in the plant bacterial wilt-resisting genetic engineering and can promote the development of the tobacco bacterial wilt-resisting genetic engineering greatly.

The invention discloses a sequence or a segment of a promoter of a cotton brassinosteroids synthetase GhDWF4 gene. The promoter contains 2600 bases and can be used for adjusting and controlling a target gene to express in a special tissue organ in a plant body so as to further adjust and control the growth of the tissue organ and obtain good agronomic traits.

The invention discloses a fertilizer for a potted flower. The fertilizer comprises the following raw materials in parts by weight: 25-35 parts of urea, 8-12 parts of potassium nitrate, 15-20 parts of zinc ammonium phosphate, 20-25 parts of potassium phosphate, 1-2 parts of calcium sulfate, 3-5 parts of magnesium humate, 1-1.5 parts of potassium silicate, 0.1-0.2 part of copper chloride, 0.03-0.05 part of sodium borate, 15-20 parts of mushroom dreg, 10-15 parts of sugar residue, 18-22 parts of peanut vine, 20-25 parts of damaged flower branch, 15-20 parts of bean cake, 10-15 parts of shell powder, 0.5-1 part of brassinosteroid, 0.1-0.3 part of indoleacetic acid, 3-4 parts of saccharomycetes and 45-55 parts of coating material, wherein the coating material comprises the following raw materials: bentonite and modified polyvinyl alcohol. The invention also discloses a preparation method of the fertilizer for the potted flower.

The invention discloses a promoter sequence of a cotton brassinolide synthase GhDWF4 gene or a fragment thereof. The promoters are 2600bp long, and they can be used to regulate the expression of target genes in special tissues and organs of plants, and then regulate the growth and development of the tissues and organs to obtain excellent agronomic traits.