16 results about "Brassinosteroid" patented technology

The application discloses a highland oat hybridization method based on oat pollen culture solution and belongs to the technical field of oat hybridization and breeding. The highland oat hybridization method comprises the following steps: (1) selecting a male parent and a female parent; (2) performing whole ear selection on the female parent; (3) performing emasculation on the female parent; (4) spraying pollen culture solution on the male spike pollen of the male parent; and (5) collecting the pollen of the male parent to perform pollination. The pollen culture solution comprises the following components: sucrose, brassinosteroid, potassium dihydrogen phosphate and calcium nitrate. The highland hybridization method is suitable for oat hybridization in a highland region, can significantly improve the vitality of oat pollen in the highland region, promotes pollen germination, thereby increasing the seed setting rate of hybridized oats and improving the hybridization success rate. Therefore, the hybridization method provides a simple and fast way for the cultivation of excellent oat varieties in the highland region.

The invention discloses a macadamia nut fruit retention and fruit setting fertilizer based on hormone synergistic regulation and an application method thereof, and relates to the technical field of fertilizers. The fertilizer comprises a first component, a second component, a third component and a fourth component, the first component is prepared from the following raw materials: urea, vermiculite, water-retaining composite gel, humic acid, pseudomonas fluorescens, actinomycetes and decomposed straw; the second component raw materials comprise stimulant, gibberellin, auxin, sodium thiosulfate, mineral oil, polyvinyl alcohol, calcium chloride, potassium silicate, boric acid and brassinosteroid; a third component raw material: a medium-nitrogen low-phosphorus high-potassium compound fertilizer and a medium-trace element fertilizer; and the fourth component comprises the following raw materials: compound sodium nitrophenolate, isopentenyl adenine, glycine betaine, mineral oil and polyvinyl alcohol. The fertilizer disclosed by the invention can provide sufficient water and nutrients in a young fruit stage, reduces the physiological fruit dropping rate in the early stage and the middle and later stages, can promote protein and fat accumulation in a fruit expansion stage and a fruit maturation stage, and reduces the physiological fruit dropping rate in the middle and later stages.

This invention relates to compositions and methods for modifying Brassinosteroid Insensitive-1 (BRI1) genes in plants by introducing at least one mutation in a region of the BRI1 gene encoding the kinase domain, optionally to improve yield traits. The invention further relates to a mutated BRI1 gene, a modified BRI1 polypeptide, and plants having increased improved yield traits produced using the methods and compositions of the invention.

The invention belongs to the technical field of plant genetic engineering and the technical field of soybean breeding, and particularly discloses application of a soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number, the CDS sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.1, the amino acid sequence of the encoded protein is shown as SEQ ID No.2, and the amino acid sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.3. The invention further discloses application of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number. The gene is located in an endoplasmic reticulum, and formation and development of lateral branches are promoted by up-regulating expression quantities of genes such as brassinolide synthesis genes CPD, ROT3 and BR6OX2, auxin response genes IAA, GH3 and SAUR and the like. The invention also discloses three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene, a soybean plant containing the gene editing mutants, and a vector construction method of the soybean plant. The invention further discloses the three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene. Experiments prove that the number of branches of homozygous gene editing mutant phenotypes of the three GmBAS1C genes is remarkably increased. The invention provides a new gene target, a molecular tool and a breeding material for soybean high-branch breeding, and has important agricultural application value.

The invention provides brassinosterols and application of an inhibitor of the brassinosterols in regulating and controlling the de novo regeneration of dicotyledonous plant root organs, and belongs to the technical field of plant growth regulation and control. According to the invention, brassinosteroid (BR) related mutants are used as materials, and the function of BR in the de novo regeneration process of roots is explored through an in-vitro leaf root regeneration induction method. The result shows that the BR signal can inhibit the root regeneration process, based on this, the invention provides the application of the brassinosteroid synthesis inhibitor in promoting generation of root organs of dicotyledonous plant in-vitro tissue and the application in dicotyledonous plant in-vitro culture, the brassinosteroid synthesis inhibitor is exogenously added, and the yield of the brassinosteroid synthesis inhibitor is improved. The rooting process of the dicotyledonous plant in-vitro leaves can be promoted, the in-vitro culture process is accelerated, and the application value is high.

The invention provides a culture medium and a culture method and application thereof. The culture medium takes an MS culture medium as a basic culture medium, and cane sugar, brassinosteroid, gibberellin, naphthylacetic acid and cystine are also added. According to the culture medium and the culture method thereof, the number of cotton ovule fiber protrusions can be increased, the weight of cotton ovule fibers can be increased, and stable production of cotton fibers obtained through in-vitro culture of cotton ovules is achieved.

The present invention relates to the field of genetic engineering technology, and in particular to the use of the GmDET2-1 protein and its encoding gene in increasing the oil content of soybean seeds. The present invention is the first to discover that the soybean brassinosteroid synthase GmDET2-1 protein or its encoding gene can regulate the oil content of plant seeds. This has high practical application value in plant variety improvement or breeding.

The application provides application of an XTH gene in promotion of grape fruit hardness formation by brassinosteroids, finds that brassinosteroids (BR) can mediate synthesis of hemicellulose in grape fruits and thus significantly improve the hardness of the grape fruits by treating the grape fruits with the brassinosteroids at a veraison stage, finds two target genes VvXTH25 and VvXTH31 capable of regulating the hardness of the grape fruits through whole genome correlation analysis and transcriptome analysis, and determines the key genes and mechanism for regulating the hardness of the grape fruits, which regulates the hardness of the grape fruits by regulating the content of hemicellulose, has the action of providing the basis and means for regulating the texture of the grape fruits and improving the storage and transportation capacity, and provides a brand-new way for truly regulating the hardness of the grape fruits.

The application discloses an agricultural composition containing hemipholine and brassinosteroid, and belongs to the technical field of agricultural plant growth regulators. The effective component of the agricultural composition provided by the application is composed of hemipholine and brassinosteroid, and the mass ratio of the hemipholine to the brassinosteroid is 2.5-5.0:0.1-1.5. After the hemipholine and the brassinosteroid are compounded and applied to corn, the quality and yield of the corn are improved, and the agricultural composition has a synergistic effect on the yield improvement of the corn within the ratio range provided by the application. After the hemipholine and the brassinosteroid are compounded and applied to wheat, the yield of the wheat can be improved and the synergistic effect is also achieved.

The present application relates to the technical field of analytical detection, and in particular to a molecular imprinting sensor for in-situ detection of brassinosteroids in plant leaves and a preparation method thereof.The present application utilizes 4-MPBA-Fc- GO-metal polyazole framework composite material and MIP to perform layer-by-layer assembly on the surface of an electrode, thereby preparing a molecular imprinting sensor for detection of brassinosteroids, and realizing high selectivity and high sensitivity detection of target molecules.This technology can effectively reduce detection cost, simplify the operation process, and improve the performance of the sensor.The molecular imprinting sensor of the present application can be used for in-vivo and in-situ analysis of brassinosteroids in plant leaves, and can realize rapid and reliable quantitative analysis in complex samples, and has a wide application prospect.

The present application belongs to the field of genetic engineering, and particularly relates to a cotton leaf-off precise regulation method based on CPD gene regulation. The present application finds that the brassinosteroid (BR) biosynthesis pathway is the key metabolic pathway for regulating the sensitivity of TDZ by analyzing the sensitivity difference of different cotton varieties to thidiazuron (TDZ) leaf-off agent, and the rate-limiting enzyme gene GhCPD is the core gene for determining the leaf-off sensitivity difference between varieties. The present application can early predict the leaf-off agent sensitivity of cotton varieties by detecting the expression level of GhCPD gene or the endogenous BR content; further, by regulating the expression level of GhCPD gene or applying exogenous 24-epibrassinolide (EBR), the leaf-off response of cotton to TDZ can be significantly enhanced.

This invention relates to compositions and methods for modifying Brassinosteroid Insensitive-1 (BRI1) genes in plants, optionally to improve yield traits. The invention further relates to plants having increased improved yield traits produced using the methods and compositions of the invention.

This invention provides cotton GoYP The application of genes in regulating plant petal size and / or petal color belongs to the field of genetic engineering technology. In this invention, GoYP Genes include GhYP Genes and / or GaYP Genes; among which, GhYP Genes can regulate the biosynthesis of flavonoids (anthocyanins and / or flavonols) in plant petals and promote petal elongation by promoting the biosynthesis of brassinosteroids (BRs). GhYP This gene encodes the R2R3-MYB regulatory factor, which has a dual function of promoting the biosynthesis of flavonoids and BRs, providing a new gene resource and a more convenient method for the synergistic improvement of the color and size of ornamental plant petals. Simultaneously, through petal-specific expression promoters... proGaFLS Driver genes GaYP Overexpression of the gene in tobacco petals resulted in redder and larger petals, further demonstrating the gene's efficacy. GoYP Its application value.

The application relates to the field of agricultural planting technology and discloses a high-yield method for spring corn close planting, which comprises the following steps: adopting film mulching drip irrigation seeding and performing normal water and fertilizer management; in the jointing stage, a nutrient solution containing self-extrusion slow-release microspheres, zinc sodium ethylenediaminetetraacetate and compound amino acids is pressed into a root area with tail water, water supply is stopped to maintain moderate water deficit, a first regulation liquid containing amine fresh ester and ethylene is sprayed on a leaf surface; in the big trumpet stage, water supply is restored and a second regulation liquid containing a compound light shield film agent is sprayed; before male flowering, soil matric potential is monitored, when reaching a drought threshold, a third regulation liquid containing 14-hydroxy brassinosteroid and fluid boron is sprayed; and in the early filling stage, a fourth regulation liquid containing the compound light shield film agent is sprayed. By introducing self-adaptive release microspheres and a film agent for improving light transmittance, combining water management and chemical regulation, the anti-lodging capacity of the close planting population is improved, the bare tip rate caused by water shortage is reduced, leaf early senescence is slowed down, and the dry matter fullness of grains is ensured.

The present application relates to brassinosteroid intermediate preparation and its purification method, belong to the field of medicine and chemical technology. The present application provides the preparation method of formula 1 brassinosteroid intermediate, reaction as follows: wherein, wherein R1 And R2 Independently selected from -COR3, C1-C8 Alkyl or R1 And R2 Together form R3 Selected from C1-C 20 Linear or branched alkyl, substituted C1-C 20 Alkyl, phenyl, substituted phenyl, preferably C1-C8 Linear or branched alkyl, phenyl or substituted phenyl, substituted phenyl is selected from the phenyl group, F, Cl, Br, nitro, methoxy arbitrary substitution C1-C6 Alkyl; comprising the following steps: step 1: formula 2 compound in solvent under the action of oxidant formula 3 obtains formula 1 compound. As described above, the preparation method of brassinosteroid intermediate of the present application, has the advantages of high selectivity, low cost and simplification of post-processing.

The present application belongs to the technical field of plant regulators, and specifically relates to the uses of 14-hydroxylated brassinosteroid and an anthocyanin extract in promoting plant growth and enhancing plant stress resistance. Provided in the present application are the uses of 14-hydroxylated brassinosteroid and the anthocyanin extract in promoting plant growth and enhancing plant stress resistance, the action concentration of 14-hydroxylated brassinosteroid being 0.001-0.1 ppm, and the action concentration of the anthocyanin extract being 0.005-0.5 ppm. In the present application, 14-hydroxylated brassinosteroid and the anthocyanin extract are used in combination at relatively low concentrations, thereby promoting plant growth, enhancing the stress-resistant capability of plants, and having a significant synergistic effect.