71 results about "Malus hupehensis" patented technology

The invention relates to B. vallismortis HSB-2 and application thereof. The preservation number of the B. vallismortis HSB-2 is CGMCC No.15305, and the sequence of 16S rDNA is shown as SEQ.ID.NO.1. The invention further relates to the application of the B. vallismortis HSB-2 to preparation of bacterial fertilizer for relieving the successive cropping obstacle of apples. The B. vallismortis HSB-2 has an obvious antagonistic effect on four types of Fusarium fusarium pathogenic bacteria causing the successive cropping obstacle of the apples; the bacterial fertilizer manufactured through the B. vallismortis HSB-2 can promote growing of the overground parts and the underground parts of Malus malus hupehensis seedlings, and at the rhizosphere, colonization can be stabilized, and growing of the four types of Fusarium fusarium pathogenic bacteria of solani, proliferatum, verticillioides and oxysporum, causing the successive cropping obstacle of the apples, in soil can be remarkably restrained;and activity of soil urease, phosphatase, sucrase and root system protective enzymes, namely SOD, POD and CAT can be remarkably improved, and the successive cropping obstacle can be relieved.

The invention discloses a malus hupehensis MhWRKY40a gene and applications thereof. The nucleotide sequence of the malus hupehensis MhWRKY40a gene is shown as the SEQ ID NO.1. The MhWRKY40a gene is capable of applying to the production of novel species, which are salt resistant and low temperature resistant, and/or plant variety improvement. A novel MhWRKY40a gene is cloned from malus hupehensis for the first time, and the homology between the gene sequence and the arabidopis thaliana, grape and barley is 50.89%. But the protein tertiary structure of the MhWRKY40a is prominently different from the homologous gene of the model plant arabidopis thaliana; the former has 5 beta-folds, while the latter has 4 beta-folds. The MhWRKY40a transgenetic tobacco has the properties of salt resistance and low-temperature resistance, raises the resistance-associated gene expression, and therefore the fact, which the MhWRKY40a gene has the function of improving the salt resistance and low-temperature resistance properties of the plants, is testified.

The invention discloses cyclocarya paliurus health-care tea. The cyclocarya paliurus health-care tea consists of the following raw materials in parts by weight: 40-80 parts of cyclocarya paliurus leaves, 10-40 parts of malus hupehensis leaves, 10-30 parts of fagopyrum tataricum leaves, and 20-50 parts of camellia sinensis leaves. The invention further discloses a preparation method of the cyclocarya paliurus health-care tea. The preparation method comprises the following steps of picking leaves, selecting materials, performing cleaning, performing drying, performing cutting to obtain strips, performing withering, performing shaking of green leaves, performing cooling, performing fixation, performing mixing, performing rolling, performing drying, performing aroma increasing, performing screening, performing artificial and careful selection and performing packing. The made cyclocarya paliurus health-care tea maintains the shape and the taste of tea leaves deeply loved by people, and besides, has the health-care efficacy of reducing blood sugar, blood fat and blood pressure, and has the characteristics of being convenient to take.

The invention relates to a method for obtaining a transgenic Malus hupehensis rehd plant without selectable marker genes. The method comprises the following steps of: culturing Agrobactrium tumefaciens carrying expression vectors in kanamycin-containing LB liquid culture medium for 16 hours for later use; culturing Malus hupehensis rehd tissue culture seedlings on a subculture medium for 35 days; cutting stretched laminae into leaf disks; placing the leaf disks into the differential culture medium to culture for 5 minutes; inoculating the leaf disks on a regeneration culture medium to culture in the dark for 1 day; connecting the leaf disks on a cephalosporin-containing differential culture medium to culture at the temperature of (25+/-1) DEG C in the dark for 14 days; culturing the leaf disks for 30 days under the conditions of photoperiod of 16-hour lighting/8-hour dark and illumination intensity of 30 umol.m<-2>.s<-1>; cutting off the obtained adventitious buds; inoculating the adventitious buds on a rooting culture medium to culture for 40 days under the conditions of photoperiod of 16-hour lighting/8-hour dark and illumination intensity of 30 umol.m<-2>.s<-1> so that the buds root; performing thermal treatment at the temperature of 42 DEG C for 15 minutes; and further identifying to obtain the transgenic Malus hupehensis rehd plant without selectable marker genes.

The invention discloses a traditional Chinese medicine preparation with the effect of lowering blood sugar, which comprises the following raw materials in parts by weight: 0.5-60 parts of malus hupehensis extract, 1-95 parts of peptide powder and 2-70 parts of oligosaccharide. The preparation method comprises the following steps: (1) preparing the malus hupehensis extract: taking the dry tree leaves of malus hupehensis, taking ethanol as a solvent, refluxing, extracting, concentrating the extract, standing below 1-4 DEG C, centrifugalizing, removing a precipitate and concentrating supernatant liquid for later use; (2) mixing: adding the peptide powder into water, mixing evenly, regulating the sugar degree, heating, then adding the malus hupehensis extract and the oligosaccharide, mixing evenly, sterilizing and drying to obtain fine powder. The traditional Chinese medicine preparation has burnt odor in smell, is sweet and bitter in taste, has obvious curative effect and good effect of lowering sugar when being used as a medicine for lowering the blood sugar, can obviously improve the clinical symptoms of mouth dryness, throat dryness, polydipsia, frequent micturition with large quantity, myasthenia of limbs, dizziness and the like and has the functions of nourishing yin, tonifying the kidney, supplementing qi, promoting the production of the body fluid and the like.

The invention discloses a tissue culture method of malus hupehensis. The method comprises five steps, namely, the explant disinfecting step, the primary culture step, the subculture step, the rootingculture step and the acclimatization and transplant step. The method has the advantages that the tissue culture rapid propagation technology is utilized; the outstanding characters of an original stock plant remain; the propagated generations are uniform; in addition, the propagation coefficients of the malus hupehensis can be efficiently and quickly increased, and the propagation cycle is reduced; moreover, the transplanting survival rate of the malus hupehensis is high. The method method meets the market demand of southern region on the malus hupehensis and is beneficial protection for the wild resource of the malus hupehensis. In addition, the culture method is easy to operate, requires low labor and time input, and is suitable for market popularization.

The invention provides a method for extracting phloridzin of malus hupehensis (pamp.) rehd. The method comprises the operation steps as follows: leaves or/and twigs of malus hupehensis (pamp.) rehd are taken and added with water for reflux extraction for three times, for the first time, 8-10 times amount of water is added for reflux extraction for longer than 60 plus or minus 5 min at the temperature of 80 plus or minus 2 DEG C, for the second time, 3-5 times amount of water is added for reflux extraction for longer than 60 plus or minus 5 min at the temperature of 80 plus or minus 2 DEG C, for the third time, 3-5 times amount of water is added for reflux extraction for longer than 60 plus or minus 5 min at the temperature of 80 plus or minus 2 DEG C, and extracting solutions of the three times are combined to obtain phloridzin. With the adoption of the method, only water is taken as a solvent, phloridzin of malus hupehensis (pamp.) rehd can still be effectively extracted, and convenience is provided for large-scale industrial production.

The invention provides a blood sugar lowering selenium-rich cyclocarya paliurus drink, which is characterized by being prepared from the following substances by weight: 40-60 parts of cyclocarya paliurus leaf, 5-15 parts of Malus hupehensis fruit, 5-15 parts of crataegus pinnatifida, 5-15 parts of Chaenomeles sinensis, 3-8 parts of Indocalamus latifolius leaf, 3-8 parts of mint leaf, 3-8 parts of selenium-containing 0.1% semen raphani, 3-8 parts of panax notoginseng, and 3-8 parts of Lonicera japonica. The preparation method consists of: weighing raw materials according to needs, conducting mixing and crushing, then adding purified water to perform infiltration, carrying out freezing and natural thawing, adding purified water after complete thawing, performing stirring extraction at 60-90DEG C for 5-12h; and concentrating the filtrate, then conducting spray drying, and carrying out subpackaging or tabletting according to the standard of 0.5-1g per part. The cyclocarya paliurus drink provided by the invention has the beneficial technical effects that: (1) the drink adopts pure natural food and medicine and edible plant resources, the processing technology is green, and the drink is not added with any chemical raw material, essence or preservative, and has no toxic or side effect; and (2) the drink has the effects of lowering blood sugar, regulating blood lipid and promoting islet functions.

The invention discloses bacillus licheniformis XNRB-3 and application thereof, and belongs to the technical field of agricultural microorganisms. The biological preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.15308 The strain provided by the invention has the functions of degrading phenolic acid substances such as phlorizin and antagonizing pathogenic bacteria, fermentation metabolites of the strain contain antifungal active substances and substances for promoting plant growth, alpha-bisabolol and 2, 4-di-tert-butylphenol have a remarkable inhibition effect on a plurality of pathogenic fungi, succinic acid has a remarkable inhibition effect on a plurality of pathogenic fungi, and the like. Monomethyl ester and dibutyl phthalate can promote root system elongation and lateral root development of arabidopsis thaliana. The strain can also be used for producing protease, cellulase, chitosanase, beta-1, 3-glucanase and the like; the bacillus subtilis secretes siderophores and auxin and has certain phosphorus dissolving, potassium dissolving and nitrogen fixing capacities. In pot culture and field tests, the addition of the strain XNRB-3 significantly promotes the growth of apple rootstock malus hupehensis seedlings and biennial apple saplings.

The invention relates to malus hupehensis leaf black tea and a production technology and a production device thereof. The production process includes the steps of cleaning, fresh leaf withering, leaf cutting, rolling, fermentation, primary drying and shaping, loosing and drying of malus hupehensis leaves after primary drying, to obtain the malus hupehensis leaf black tea. The production device comprises a cleaning part, a dehydration and withering part, a leaf cutting and fermentation part, a primary drying part and a shaping and loosing part, and all the parts are connected through conveyors or lifting machines. The produced malus hupehensis leaf black tea has strong smell, and the fermentation quality of the malus hupehensis leaves in a fermentation room is ensured; aiming at a condition of relatively thick Hubei malus hupehensis leaves, relatively good effect can be obtained through about 20 times of shaping and loosing treatment; and with the production device continuous automatic production mode, the production efficiency is improved and the cost of production is reduced.

The present invention belongs to the technical field of health-care products and more particularly relates to a composition containing radix puerariae and an extraction device and a method thereof. The composition comprises the following raw material components in parts by weight: 5-30 parts of radix puerariae, 0-25 parts of green tea, 0-10 parts of malus hupehensis, 0-15 parts of radix polygonati officinalis, 0-20 parts of rhizoma polygonati and 0-40 parts of pine needles. The extraction device comprises a microwave extraction chamber and an ultrasonic extraction chamber. The extraction method is as follows: freeze-drying and crushing treating of the radix puerariae, respective vacuum freezing, withering treating, vacuum microwave enzyme deactivating and drying treating of the malus hupehensis and fresh green tea leaves, ultralow temperature freeze-drying of herba lophatheri and lotus leaves, and freeze-drying and crushing treating of sliced licorice. The efficacy components are sufficiently preserved, so that the composition is beneficial to absorption of human body, has the functions of lowering blood fat and lowering blood pressure, and is suitable for uses of diabetic patients.

The invention belongs to the technical field of production of edible mushrooms, particularly relates to oyster mushroom compost and further relates to a preparation method of the oyster mushroom compost. The oyster mushroom compost is characterized by comprising components in parts by weight as follows: bran, peony seed shells, soybean meal, corn stalk, leaves of sesbania, gypsum powder, sugar, animal manure, rice husk, corncob, wood flour of sawtooth oak, sesame meal, wood flour of aspens and leaves of malus hupehensis. The oyster mushroom compost prepared with the method facilitates growth of oyster mushrooms, and the biological efficiency of the oyster mushrooms is high and can reach about 110%; besides, leaves of the sesbania which is a common plant growing in waste land are often abandoned, the leaves of the sesbania are adopted in the method, accordingly, waste is turned into wealth, resources are saved, the leaves of the sesbania are used reasonably, the disease-resistant and pest-resistant capacity of the leaves of the sesbania is high, pest and disease damage to the oyster mushrooms is prevented, and damage of mites, molds and maggots to the oyster mushrooms is reduced.

The invention provides a beverage with effects of reducing blood glucose and blood fat. The beverage is characterized by comprising the following raw materials: cyclocarya paliurus, malus hupehensis, radix polygonati officinalis and chaenomeles speciosa. A method for preparing the beverage comprises the following steps: (1) grinding the cyclocarya paliurus, malus hupehensis, radix polygonati officinalis and chaenomeles speciosa to obtain raw material powder; (2) mixing the raw material powder obtained in the step (1) with water, heating and refluxing to obtain filtrate; (3) mixing the filtrate obtained in the step (2) with a flocculating agent for flocculating, heating and filtering to obtain clear liquid; and (4) diluting the clear liquid obtained in the step (3), flavoring, sterilizing and filling to obtain the beverage. The preparation method provided by the invention is simple and quick, and is suitable for large-scale production; and the obtained beverage is clear and stable, does not have precipitate, and has remarkable effects of reducing blood glucose and blood fat.

The invention discloses a making technology of malus hupehensis leaf tea, and relates to the technical field of tea leaves. The making technology comprises the preparation processes that harvested fresh leaves are spread to be aired for no longer than 12 hours and then subjected to short-time fixation for 30-120 seconds at the high temperature ranging from 150 DEG C to 350 DEG C, the activity of oxidase in the fresh leaves is destroyed, and the condition that the phlorizin content is decreased is prevented; the malus hupehensis leaves obtained after fixation are baked for 1-60 minutes at the temperature ranging from 60 DEG C to 250 DEG C, and then the dry malus hupehensis leaf tea with the water content lower than 10% can be produced. The making technology is simple in preparation process, economical, practical and low in processing cost, and the made malus hupehensis leaf tea has the weak characteristic aroma, contains the high-content phlorizin efficacy ingredient and is stable, safe and reliable in quality.

The invention provides food or a healthcare product or a medicine composition containing lycium barbarum leaves and reducing blood glucose. The food or the healthcare product or the medicine composition comprises lycium barbarum leaves and malus hupehensis leaves according to the weight ratio of (1-10) : (1-10). It is found that after lycium barbarum leaves and malus hupehensis leaves are combined for use, the synergistic interaction effect is achieved, hypoglycemic activity is improved, and the effect is superior to that achieved through independent use of lycium barbarum leaves and malus hupehensis leaves through research. Based on the research, the novel composition reducing blood glucose is provided for providing a new choice for clinical medication and healthcare treatment.

The invention relates to a composition. The composition comprises folium mori, ampelopsis grossedentata, malus hupehensis rehd and the like. The application of the composition comprises prevention andtreatment of hyperglycemia. The composition belongs to the fields of food and medicine.

The invention discloses a medicine used for curing infantile malnutrition and its preparation method thereof. The medicine is prepared with small centipede herb, Clarias fuscus, Malus hupehensis, Coix lacryma-jobi L.var.monilifer Watt, negundo chastetree fruit, maggot, scabrid small hooked hedyotis herb, sugar cane juice and effective microorganism original liquid. The small centipede herb can dispel the wind, unchoke orifices, remove toxicity for detumescence, dissipate blood stasis, dislodge the nebula film and help to eliminate the infantile malnutrition according to the drug theory and long-term treatment experience. As the Clarias fuscus raises blood deficiency and prospers the male, it is suitable to treat the malnutrition caused by long-term illness and weakness. Coix lacryma-jobi L.var.monilifer Watt and maggot can clear heat and cool blood, strengthen the spleen, disperse food retention, kill parasites and especially treat the infantile and parasitic malnutrition with abdominal bloating and prominent veins. Malus hupehensis and scabrid small hooked hedyotis herb harmonize the stomach, strengthen the spleen and clear dampness, which can be applied in curing infantile malnutrition caused by dampness stagnation and accumulation with high efficiency.

The invention provides a blood sugar reducing food containing fructus lycii or a blood sugar reducing health care product containing fructus lycii or a blood sugar reducing composition containing fructus lycii. The blood sugar reducing composition comprises, by weight, 1-10 parts of fructus lycii, 10-20 parts of malus hupehensis leaves and 1-10 parts of thorn pears. By means of the study, the fructus lycii and the malus hupehensis leaves has a synergistic effect after being used in cooperation, the hypoglycemic activity is improved and is superior to the effect achieved when the fructus lycii and the malus hupehensis leaves are used independently. The blood sugar reducing effect is better after the fructus lycii and the malus hupehensis leaves are combined according to the specific proportion (5:15) and is superior to the effect achieved when the fructus lycii and the malus hupehensis leaves are combined according to the other proportions (1:10 and 10:10). Based on the study, the new blood sugar reducing composition is provided, and a new choice is provided for clinical medication and health care treatment.

The invention belongs to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for treating brain glioma as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following ingredients by weight: 20 parts of ganoderma, 6 parts of mint, 10 parts of cenchrus echinatus, 10 parts of purslane, 10 parts of malus hupehensis leaf, 20 parts of astragalus membranaceus, 15 parts of ligusticum wallichii, 13 parts of hairyvein agrimonia herb and bud, 5 parts of turmeric, 5 parts of lily, 10 parts of radix paeoniae rubra, 12 parts of radix codonopsitis, 8 parts of panax notoginseng, 9 parts of coptis chinensis, 11 parts of centella, 4 parts of lucid asparagus, and 10 parts of poria cocos. The traditional Chinese medicine composition has an excellent curative effect on treating brain glioma, and has a remarkable clinical popularization value.

The invention relates to a beverage used for blending and diluting whisky and a preparation method thereof. The beverage is composed of 50-55 parts of leaching solution of malus hupehensis leaves, 30-35 parts of kudzuvine flower leaching solution and 10-15 parts of an auxiliary material. The preparation method comprises the following steps: respectively washing, drying, crushing, heating and lixiviating malus hupehensis leaves and kudzuvine flowers, mixing and cooling the obtained leaching solution, adding the auxiliary material, homogenizing, cooling, filtering and sterilizing to finally obtain the beverage used for blending and diluting whisky. The beverage of the invention is golden yellow, uniform, fresh and sweet and sourish, clear and bright, and the beverage has gentle taste and health-care effects of relieving heat and quenching thirst, resisting fatigue, reducing alcoholic toxicity and the like on a human body.

The invention relates to a method and device for planting Hanfu apple trees capable of being transplanted in four seasons for landscaping, and belongs to the technical field of horticultural techniques. The planting method comprises the steps that a Hanfu apple variety with the self-fruitful capability is selected; Hanfu apple scions are grafted on a GM256/malus hupehensis rootstock at a high position in a nursery; the planting device is laid, roots of seedlings are placed in planting pits of the planting device after fully extending, and the planting pits are filled with a cultivation substrate, compacted, watered and covered mulching film; all main branches are removed during pruning in winter in two continuous years, and the main branches sprouting in the third year are formed through branch stretching. According to the method, the Hanfu apple trees with the self-fruitful capability serve as main test materials and are subjected to high-position grafting on an interstock GM256, malus hupehensis serves as a base stock, the tree vigor is kept strong, and formation of a large quantity of flower buds can be guaranteed. The planting device for limiting the rooting zone and a soil, water and fertilizer management and shaping and trimming technology for controlling growth are also used so that both the landscape shape and fruiting of the trees during the four-season transplanting can be achieved.

The invention relates to a formula and a preparation process of a plant substitutional tea health product. The formula and the preparation process of the plant substitutional tea health product are researched on the basis of the existing substitutional tea. The plant substitutional tea health product contains golden camellia, sanchi flowers, eucommia male flowers, malus hupehensis leaves, ampelopsis grossedentata leaves, cyclocarya paliurus leaves, sea buckthorn fruits, Chinese olive fruits and gingko fruits, and has the effects of the three kinds of flowers, three kinds of leaves and three kinds of fruits by virtue of perfect combination of traditional processes of new resource food raw materials. The plant substitutional tea health product is suitable for all people and has the characteristics of improving immunity, enhancing constitution and the like.

The invention discloses compound Hubei crabapple leaf functional food for assisting in reducing blood sugar and reducing blood lipid and a preparation method of the functional food. The functional food comprises, by mass, 1-50 parts of Hubei crabapple leaves, 5-80 parts of polygonatum kingianum, 3-70 parts of mulberry leaves, 1-60 parts of silkworm pupae and 1-50 parts of sessileflower acanthopanax bark. The preparation method includes the steps that after dried raw materials are preprocessed, water is added for reflux extraction, an extracting solution is concentrated into thick paste, maltodextrin is added into the thick paste, a soft material is prepared, granulated, dried and shaped to obtain total particles, and the functional food is obtained. Researches show that after all the raw materials are combined for use, the synergistic function is achieved, and the functions of reducing blood sugar and blood lipid are remarkable.

The present invention relates to a production technology of eurotium cristatum malus hupehensis tea. A PDA potato culture medium is bottled; the culture medium is inoculated with 4-6 eurotium cristatum blocks; the eurotium cristatum is subjected to static culture and shaker culture; and an eurotium cristatum liquid strain is manufactured; malus hupehensis leaves are washed cleaned; the washed malus hupehensis leaves are dried or sun-dried; black tea is soaked using boiling water; the soaked black tea is filtered to obtain a filtrate; the malus hupehensis leaves are soaked in the black tea filtrate; the soaked malus hupehensis leaves are taken out; the taken out malus hupehensis leaves are packaged using thin polypropylene film bags; the packaged malus hupehensis leaves are sterilized; themalus hupehensis leaves are inoculated with the eurotium cristatum liquid strain; the eurotium cristatum liquid strain is cultured and fermented until the eurotium cristatum grows fully on inside andoutside the malus hupehensis leaf culture medium; the cultured and fermented malus hupehensis leaves are baked; and the baked malus hupehensis leaves are packaged to obtain the eurotium cristatum malus hupehensis tea. Specific malus hupehensis leaves in Shennongjia are used as a raw material; the treated malus hupehensis tea is inoculated with the eurotium cristatum for fermentation; the fermentation by the eurotium cristatum can degrade tannin, alkaloid, etc., and starch; and the obtained eurotium cristatum malus hupehensis tea has a brownish red color after brewing, is free of crude green taste of traditional malus hupehensis tea and sweet in taste, and has effects of promoting digestion, lowering blood lipid, dissolving fat, regulating sugar metabolism, etc.

The invention provides a Malus hupehensis and Malus micromalus Makino seed refrigerator lamination method. The method comprises the following steps: selecting healthy and plum Malus hupehensis and Malus micromalus Makino seeds collected in autumn, immersing the selected seeds in a 1.5% sodium hypochlorite solution for 15 min, airing the immersed seeds, processing the seeds at three different lamination times, putting a matrix block in an aperture disk, thoroughly watering the matrix block in the laminating process twice, sowing the seeds in the matrix block, laminating the seeds in a 0 DEG C refrigerator, taking out the seeds in the next spring, carrying out germination rate statistics, and determining the optimal lamination time according to the germination rate in order to construct Malus hupehensis and Malus micromalus Makino seed refrigerator lamination technology standards and select the time with high emergence rate as the determined lamination time. The method has the advantages of convenience in operation, controllable conditions, standardized and consistent seedling emergence, high emergence rate, and convenience for apple stock test and high-efficiency lamination of seeds in the factory seedling growing process, and is suitable for being applied as a stock seed refrigeration lamination method.