5results about "Glycoside steroids" patented technology

The application discloses a process for converting ginsenoside into rare ginsenoside by a microbial method, and relates to the technical field of ginsenoside, and comprises the following steps: step S1, taking ginseng rootlet powder, putting the ginseng rootlet powder into extraction cylinders of Soxhlet extractors in 10 times, adding an ethanol solution and an organic acid mixed solution into the extraction cylinders, and placing the extraction bottles in an oil bath at a certain temperature to carry out reflux extraction to obtain ginsenoside extraction crude powder; step S2, microbial conversion: adding the ginsenoside crude powder obtained in the step S1 into LB liquid culture medium, and adding fiber bacteria 72-3 bacterial liquid in a proportion of 0.5-2.0 %, and using the newly separated fiber bacteria 72-3 to ferment and convert the ginsenoside into rare ginsenoside, and first, the conversion is carried out through 48 hours of culture at 37 degrees; and step S3, further enrichment, adsorption, elution and filtration of the ginsenoside through a macroporous resin column and a silica gel chromatographic column, vacuum drying and concentration and crystallization, and finally, a mixture containing rare ginsenoside hydrolysis products Rh1 and F1 is obtained.

The application discloses a method for increasing the extraction of saccharide components and / or glycoside components, which comprises the following steps: (1) preparing a sugar solution and / or a sugar alcohol solution; (2) soaking raw materials in the sugar solution and / or the sugar alcohol solution; and (3) heating the raw materials in the sugar solution and / or the sugar alcohol solution for 1-3 times, and obtaining an extract by filtration. The above method can significantly increase the extraction rate of saccharide components and / or glycoside components in the raw materials, thereby helping to improve the utilization rate and curative effect of the raw materials.

The present invention relates to the field of nucleic acid sequencing. In particular, the present invention relates to a scanning reagent comprising a saponin compound, a kit comprising the scanning reagent and a method for nucleic acid sequencing by using the scanning reagent.

Provided are isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful for mogroside biosynthesis. The mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS), and udp-glucosyltransferase (UGT). Also provided are methods of producing mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and / or glycosylated mogrol (mogroside) with at least one UDP glucose glucosyltransferase (UGT) enzyme polypeptide of the invention that catalyzes glucosylation of the mogrol and / or glycosylated mogrol to produce a mogroside with an additional glucosyl component, thereby producing a mogroside. Alternatively or additionally provided are methods of synthesizing mogrol, the methods comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein that catalyze synthesis of mogrol from the mogrol precursor substrate, thereby synthesizing mogrol.

This invention relates to the field of eutectic technology, and particularly to a ginsenoside-mannitol eutectic, its preparation method, and its applications. The XRD pattern of this eutectic exhibits characteristic diffraction peaks at at least 13.80°, 17.45°, 18.90°, 20.03°, 20.60°, 21.41°, 21.54°, 28.30°, 34.13°, and 44.08° at 2θ angles, with an error tolerance of ±0.2°. The DSC pattern shows characteristic endothermic peaks at 168.4℃ and 359℃, with an error tolerance of ±0.2℃. The preparation method is as follows: mannitol is dissolved in pure water, and ginsenosides are dissolved in ethanol. The mixture is then homogenized, subjected to high-pressure reaction or ultrasonic treatment in a reactor, allowed to stand, crystallized, filtered, dried, and ground. The obtained eutectic can be used in cosmetics, food, health products, and pharmaceuticals. The eutectic of the present invention has the advantages of being non-irritating, highly safe, easily absorbed, and having good stability.