102results about "Glycoside steroids" patented technology

The invention discloses radix notoginseng stem and leaf rare ginsenoside as well as a preparation method and application thereof, and belongs to the technical field of ginsenoside. The radix notoginseng stem and leaf rare ginsenoside comprises Rk3, Rh4, S-Rg3, R-Rg3, Rk1 and Rg5. The radix notoginseng stem and leaf rare ginsenoside can be used for preparing radix notoginseng stem and leaf rare ginsenoside. The preparation method comprises the following steps: mixing the pseudo-ginseng stem and leaf extract with an acetic acid aqueous solution, reacting at 105-120 DEG C for 10-40 minutes, and drying; in the preparation process, macroporous resin purification is not carried out. According to the scheme, part of chemical substances in the panax notoginseng stem and leaf extract can be rapidly converted at a low temperature, new active substances Rh4, S-Rg3, R-Rg3, Rk1 and Rg5 with high purity are obtained at the same time, and the obtained panax notoginseng stem and leaf rare ginsenoside can effectively inhibit propionibacterium acnes, burkholderia cepacia and malassezia furfur.

The invention belongs to the technical field of active substance preparation, and particularly relates to a preparation method and application of high-purity rare ginsenoside CK. The high-purity rare ginsenoside CK with the content exceeding 95% is finally produced through the steps of combined enzymolysis, ultrafiltration, nanofiltration, multiple crystallization and the like, the production efficiency and purity of the rare ginsenoside CK are improved through the preparation method, only ethyl alcohol and water are used as solvents in the whole process, it is guaranteed that the technology is green and environmentally friendly, and the method is suitable for industrial production. The method has the advantages of simple operation, no use of harmful chemical solvents, great reduction of environmental pollution, realization of the significant cost advantage in the whole production process due to repeated use of equipment and solvents, suitableness for large-scale industrial application, and wide market prospects and economic benefits.

The invention discloses a preparation method of 20 (E)-ginsenoside F4 and application of the 20 (E)-ginsenoside F4 in preparation of anti-liver injury medicines, and relates to the technical field of biological medicines. The rare ginsenoside 20 (E)-ginsenoside F4 is prepared and purified from ginsenoside Re through an acid catalytic conversion method, and the rare ginsenoside 20 (E)-ginsenoside F4 shows remarkable advantages and wide prospects in research and development of liver protection drugs. The monomer has strong liver protection activity, complies with the development trend of monomeric compound preparations, and provides efficient active ingredients for liver protection drugs. The 20 (E)-ginsenoside F4 with efficient activity, excellent safety and environment-friendly preparation characteristics can be widely applied to preparation of liver protection drugs or pharmaceutical compositions, especially drugs or pharmaceutical compositions for treating liver injury caused by chemotherapeutic drugs.

Disclosed is a method of synthesizing a compound selected from the group consisting of C-alkyl glycosides, C-alkenyl glycosides, C-heteroaryl glycosides, Se-glycosides and S-glycosides, the method comprising: a. Reacting a natural unprotected sugar with a 2-chloro-1, 1, 1, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3 The method comprises the following steps: reacting 1, 3-dimethylimidazoline chloride (DMC) and a mixture of heteroaryl mercaptan and trimethylamine (Et3N) in a mixture of water and dialkane to generate heteroaryl glycosyl thioether; b, under the irradiation of a blue light LED, in the presence of Hantzsch ester (HE), 1, 4-diazabicyclo [2.2. 2] octane (DABCO) and DMSO, heteroaryl glycosyl thioether and a reagent are subjected to light-induced cross coupling, and the compound is generated.

The invention provides a method for extracting ginseng tissue culture adventitious root saponin by a eutectic solvent synergistic biological enzymolysis technology, which comprises the following steps: (1) adding ginseng tissue culture adventitious root dry powder into water, adding compound enzyme, heating, adjusting the pH value of the solution, and carrying out biological enzymolysis; after the enzymolysis is finished, performing enzyme deactivation and centrifugation to obtain supernate A and precipitate; (2) adding an eutectic solvent into the precipitate, and performing ultrasonic extraction and centrifugation to obtain supernate B; and (3) combining the supernate A and the supernate B, and freeze-drying to obtain the ginseng tissue culture adventitious root saponin. According to the method, the biological enzymolysis technology and the eutectic solvent extraction technology are combined, the content of total saponins in the ginseng tissue culture adventitious root extract is increased, and meanwhile the content of Rg3, Rg5 and Rh2 rare ginsenosides in the ginseng tissue culture adventitious root extract is also increased. The invention belongs to the technical field of plant component extraction.

The invention discloses a method for preparing high-purity ginsenoside Rh2, panoxadiol type total saponins extracted and separated from ginseng roots or ginseng stems and leaves are taken as a substrate, two-step combined enzyme hydrolysis is utilized to convert a diol type ginsenoside mixture into rare ginsenoside Rh2, and the ginsenoside Rh2 with the purity of more than or equal to 98.5% is obtained by recrystallizing the product. The preparation method has industrial prospects, and the product can be used as a raw material of medicines and other products.

The invention belongs to the technical field of biological medicines, and provides a method for synthesizing testosterone 17-O-beta-D-glucosyl-(1-> 4)-6 ''-O-acetyl-beta-D-glucoside through two-step enzymatic coupling. Mainly relates to an intermediate compound testosterone 17-O-beta-D-glucosyl-(1-> 4)-beta-D-glucoside for synthesizing the molecule, mutant amino acid and nucleotide sequences of glycosyl transferase OsSGT2 for synthesizing the intermediate, and synthesis application. In addition, the invention also provides an application of the testosterone 17-O-beta-D-glucosyl-(1-> 4)-6 ''-O-acetyl-beta-D-glucoside in liver protection.

A method for synthesizing compounds selected from the group consisting of C-alkyl glycosides, C-alkenyl glycosides, C-heteroaryl glycosides, Se-glycosides, and S-glycosides is disclosed, comprising: a. reacting a natural unprotected sugar with a mixture of 2-chloro-1,3-dimethylimidazolinium chloride (DMC), heteroarylthiol, and trimethylamine (Et3N) in a mixture of water and dioxane to produce a heteroarylglycosyl sulfide; and b. photo-induced cross-coupling of the heteroarylglycosyl sulfide with reagents under blue LED irradiation in the presence of hantchu ester (HE), 1,4-diazabicyclo[2.2.2]octane (DABCO), and DMSO to produce the compound.

The invention discloses a preparation method of mogroside, which comprises the following steps: taking fresh siraitia grosvenorii pulp, and carrying out flash extraction with alkaline water at the temperature of 10-15 DEG C; placing the treated pulp in a flash extractor, extracting twice in a solvent, and combining filtrates extracted twice to obtain a total filtrate; performing composite column chromatography on the total filtrate, eluting with pure water, combining the collected effluent and water washing liquid, and concentrating the solution; adding high-activity yeast into the obtained solution, fermenting for 4-6 hours at the temperature of 30-32 DEG C, then inactivating, cooling, filtering, passing through macroporous resin chromatography, sequentially eluting with pure water, acetone and ethanol, collecting ethanol eluant, and concentrating at low temperature to obtain concentrated solution; and adding pure methanol or absolute ethyl alcohol into the obtained concentrated solution, crystallizing under sealing and stirring conditions, carrying out suction filtration to obtain crystals, washing, dissolving with pure water, concentrating, and carrying out spray drying to obtain the mogroside. According to the method, the mogroside product with few impurities and high mogroside V content can be obtained.

The present disclosure relates to compositions and methods for the production of transformed plants and other organisms with increased production of mogroside compounds, particularly mogroside V, and the mogroside compounds, plants and plant parts so obtained.

To provide alternative and / or improved sweetness modifying composition.SOLUTION: There is provided a sweetness modifying composition comprising: one or more high-intensity sweetener(s) selected from steviol glycosides and / or mogrosides; and one or more low-intensity sweetener(s) selected from cellobiose, psicose, cyclamate and / or 11-O-mogroside V, wherein the sweetness modifying composition increases the sweetness of a sweetened composition by more than the sweetness of the sweetness modifying composition alone; and / or wherein the ratio of the one or more high-intensity sweetener(s) to the one or more low-potency sweetener(s) ranges from about 2:1 to about 12:1.SELECTED DRAWING: None

A type based on 3 O A method for stereoselectively synthesizing α-oxyglycosides using a quinaldine ester glycan donor, comprising the steps of: using 3- O Quinaldine ester glucalose is a sugar donor, a catalyst, a ligand, triethylamine and a sugar acceptor and an organic solvent are added, and the reaction is stirred at 100-120 ° C. The reaction progress is monitored by TLC. After the reaction is carried out for 20-24 hours, the reaction is terminated to obtain an α-2,3-unsaturated oxygen glycoside. The technical solution of the present invention preferentially generates a product with a β configuration during the glycosidation reaction. Under the action of high temperature and palladium catalyst, the original β-glycosidic bond is broken and regenerated with an α configuration to obtain a thermodynamically stable α-2,3-unsaturated oxygen glycoside. The method has a wide range of substrate applicability, and the stereoselectivity of most substrates can reach at least α:β=10:1.

The invention provides a ginsenoside composition for accelerating acetaldehyde metabolism as well as a preparation method and application thereof, and particularly belongs to the technical field of medicine preparation. The invention provides a ginsenoside composition. The ginsenoside composition is prepared from the following components in parts by weight: 0.5 to 25 parts of Rg5, 0.5 to 20 parts of Rk1, 0.5 to 15 parts of Rh4, 0.5 to 10 parts of Rk3 and 0.5 to 10 parts of 20 (S)-Rg3. The ginsenoside composition can improve the enzymatic activity of acetaldehyde dehydrogenase, accelerate acetaldehyde metabolism and accelerate removal of acetaldehyde in a human body, and is beneficial to reduction of liver injury, protection of a cardiovascular system, protection of a nervous system, reduction of inflammatory response and improvement of the overall metabolic function.

The present disclosure relates to an ester derivative prodrug of inotodiol and use thereof. The prodrug not only has increased solubility with respect to an inorganic solvent and an organic solvent, but also has improved stability, and thus can be used in the treatment of various inflammatory diseases, including inflammatory diseases or allergic diseases.

The invention belongs to the technical field of separation methods of new natural compounds, and particularly relates to a separation method of new ginsenoside in ginseng. According to the separation method, ginseng roots serve as raw materials, sample segmentation and targeted enrichment of target compounds are carried out through ultrasonic extraction, normal-pressure and medium-pressure preparation and the like, then enrichment is further carried out through high-pressure semi-preparation, specific parameters are adopted in each step, and therefore the new ginsenoside can be separated from the ginseng roots. The discovery of the new ginsenoside is helpful for deeply exploring the action mechanism of the ginseng, and also possibly provides new active substances for disease treatment, so that the pharmaceutical application of the ginseng is further developed, and the economic value of the ginseng is improved.

The invention discloses a method for promoting conversion of rare ginsenoside in gynostemma pentaphylla and a therapeutic effect of a prepared product on metabolic syndrome. Specifically, the invention discloses a method for promoting conversion of rare ginsenoside in gynostemma pentaphylla, and the method comprises the following steps: (1) taking a proper amount of gynostemma pentaphylla medicinal material dried in the shade, crushing, and adding water for infiltration; (2) soaking for a period of time at a certain water bath temperature; (3) evaporating to dryness, adding ethanol or methanol with a certain concentration, carrying out heating reflux extraction or ultrasonic extraction, cooling an extracting solution, filtering, and carrying out vacuum concentration to obtain a concentrate; and (4) loading the concentrate on macroporous resin, adsorbing, eluting, and collecting the 60-80% ethanol elution part to obtain the total saponins dried in the shade. The method is mild in reaction condition, high in efficiency, high in specificity and free of pollution, and the prepared product is high in rare ginsenoside content and can be used for preventing and / or treating metabolic diseases such as non-alcoholic fatty liver diseases.

This invention relates to intermediates, preparation methods, and uses of pregnanediol P57 and its derivatives as shown in Formula I, as well as pregnanediol P57 derivatives. The method employs a convergent synthesis strategy to synthesize P57 and its derivatives, resulting in a short synthetic route, high yield, and suitability for industrial production. The definitions of each substituent in Formula I are the same as those in the claims.

The invention belongs to the technical field of biological medicine, and particularly relates to an antioxidant and anti-aging ginseng composition, a preparation method and application. The composition is composed of ginsenoside Rk3, Dehydroprotopanaxatrial II and ginsenoside Rk2 according to a specific proportion, and during preparation, ginseng fibrous roots are used as raw materials and treated through a microwave-thermochemical synergistic gradual conversion method, and then high-purity components are obtained through rapid medium-pressure preparative chromatography and semi-preparative high performance liquid chromatography purification. Experiments show that the composition can significantly reduce MDA and PC contents of oxidative injury mice, improve SOD and GSH levels, and relieve liver injury; when being externally applied, the composition can improve skin photoaging induced by UVB, improve skin moisture, increase collagenous fibers, reduce oxidative stress level and inhibit inflammatory factors. The process is efficient and controllable, and the product is suitable for oral oxidation resistance and external aging resistance and has huge application value.

The invention discloses a method for extracting ginsenosides Rb1 and Rd from pseudo-ginseng. The method comprises the following steps: step 1, extracting raw materials; step 2, recovering ethanol; step 3, preparing crude extraction column loading liquid; step 4, crude extraction; step 5, re-dissolving; step 6, separating a single component; step 7, refining a single component of ginsenoside Rb1; and step 8, refining a single component of ginsenoside Rd. According to the method disclosed by the invention, the non-polar macroporous adsorption resin and the moderately polar macroporous adsorption resin are combined for use, gradient elution is performed, and a high-content and batch-level single-component product can be obtained; on the basis, a high-content product is further obtained through the steps of crystallization and chromatography. The method disclosed by the invention is easy to operate and implement, can be repeatedly used and is relatively high in yield.

The invention belongs to the technical field of chemical synthesis, and discloses a glycosyl donor containing an olefin structure and application of the glycosyl donor, the glycosyl donor has a structure as shown in a formula I, Gly is glycosyl with one or more hydroxyl groups protected by protecting groups on a sugar ring; x is an oxygen atom or a methylene group; and n is 0, 1 or 2. By improving the chemical structure of the glycosyl donor, the obtained glycosyl donor containing the olefin structure as shown in the formula I can be applied to glycosylation reaction, particularly, the glycosyl donor can be activated through a catalytic amount of acid, mild and efficient glycosidic bond construction is achieved, and a glycosylation product is prepared.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to an extraction method of a high-activity ginseng extract, which is used for improving the conversion and extraction efficiency of rare ginsenoside Rg1, Rg3, Rg5, Rh2 and Compound K. The extraction method combines microbial fermentation, electrostatic field wall breaking and supercritical CO2 extraction, the high-voltage pulse electric field induces ginseng cells to generate an electroporation effect, permeability of cell membranes and cell walls is improved, supercritical CO2 extraction has high cell permeability, the extraction rate and purity of ginsenoside are improved, and in addition, a catalytic promoter added in the extraction process is beneficial to conversion catalysis of rare ginsenoside components.

This invention discloses a ginsenoside Rg5 derivative with the molecular formula: C 42 H 74 O 12 The molecular weight is 770.5180. Furthermore, this invention also discloses the synthesis method and application of this derivative. The synthesis method of this invention is simple and safe, and the synthesized ginsenoside Rg5 derivative exhibits strong stability and superior therapeutic effect on non-alcoholic steatohepatitis (NAH). This invention is the first to combine the prepared ginsenoside Rg5 derivative with the PDE4 inhibitor (R)-(-)-Rolipram, showing significantly better efficacy than either drug alone. When the molar ratio of the two drugs is 1:1, the drug exhibits even better therapeutic effect on NHA, showing promising application prospects.

The application discloses a method for promoting transformation of rare ginsenoside in gynostemma pentaphyllum, and comprises the following steps: (1) taking a proper amount of dried gynostemma pentaphyllum medicinal material, crushing and then adding water for soaking; (2) soaking for a period of time under a certain water bath temperature; (3) after steaming and drying water, adding a certain concentration of ethanol or methanol, heating and refluxing extraction or ultrasonic extraction, filtering after cooling the extract, reducing pressure concentration, and obtaining a concentrate; and (4) loading the concentrate on a macroporous resin, adsorbing, eluting, collecting 60-80% ethanol elution part, and obtaining dried total saponin. The method has the advantages of mild reaction condition, high efficiency, strong specificity, no pollution, high content of prepared product rare ginsenoside, and can be used for preventing and / or treating metabolic diseases such as non-alcoholic fatty liver disease.

The application discloses a process for converting ginsenoside into rare ginsenoside by a microbial method, and relates to the technical field of ginsenoside, and comprises the following steps: step S1, taking ginseng rootlet powder, putting the ginseng rootlet powder into extraction cylinders of Soxhlet extractors in 10 times, adding an ethanol solution and an organic acid mixed solution into the extraction cylinders, and placing the extraction bottles in an oil bath at a certain temperature to carry out reflux extraction to obtain ginsenoside extraction crude powder; step S2, microbial conversion: adding the ginsenoside crude powder obtained in the step S1 into LB liquid culture medium, and adding fiber bacteria 72-3 bacterial liquid in a proportion of 0.5-2.0 %, and using the newly separated fiber bacteria 72-3 to ferment and convert the ginsenoside into rare ginsenoside, and first, the conversion is carried out through 48 hours of culture at 37 degrees; and step S3, further enrichment, adsorption, elution and filtration of the ginsenoside through a macroporous resin column and a silica gel chromatographic column, vacuum drying and concentration and crystallization, and finally, a mixture containing rare ginsenoside hydrolysis products Rh1 and F1 is obtained.

The invention discloses a deep-eutectic solvent cellulase system and application of the deep-eutectic solvent cellulase system in extraction of saponin components in ginseng. The deep-eutectic solvent cellulase system contains a deep-eutectic solvent, cellulase and an H3C6H5O7 / Na3C6H5O7 buffer solution, the eutectic solvent is composed of a hydrogen bond donor and a hydrogen bond acceptor, and the hydrogen bond donor is D-sorbitol, glycerin, 1, 4-butanediol, L-proline, urea, ethylene glycol or xylitol; the hydrogen bond acceptor is betaine, choline chloride, benzyl tributyl ammonium chloride, tetrabutyl ammonium chloride or benzyl triethyl ammonium chloride. Under the optimal DESs extraction, the content of ginsenoside is two times that of methanol extraction, the extraction efficiency is greatly improved, and the conversion value is higher.

The present invention discloses a preparation method and application of an American ginseng extract rich in rare ginsenosides, belonging to the technical field of rare ginsenoside preparation. The method solves the problem that the content of Rg3, Rg5, and Rk1 in existing extracts needs to be improved. First, the root of American ginseng is sliced ​​or crushed and mixed with water, and boiled for 2-5 hours; (2) a 50%-70% ethanol solution is added at 40-80°C for soaking and filtering, and the filter cake is further added with a 50%-70% ethanol solution at 40-80°C for soaking and filtering, the filtrate is combined, heated at 120-140°C for concentration, and ethanol is recovered; (3) the concentrate is applied to a macroporous resin column, first rinsed with 3 column volumes of water, then eluted with 60-95% ethanol, the alcohol phase eluate is combined, heated at 120-135°C for concentration, and ethanol is recovered to obtain an American ginseng extract rich in rare ginsenosides Rg3, Rg5, and Rk1, which can be used in functional foods, health products, and biopharmaceutical products.

The invention discloses a preparation method of 20 (Z)-ginsenoside F4 and application of the 20 (Z)-ginsenoside F4 in preparation of anti-liver injury medicines, and relates to the technical field of biological medicines. The rare ginsenoside 20 (Z)-ginsenoside F4 is prepared and purified from ginsenoside Re through an acid catalytic conversion method, and the rare ginsenoside 20 (Z)-ginsenoside F4 shows remarkable advantages and wide prospects in research and development of liver protection drugs. The monomer has strong liver protection activity, complies with the development trend of monomeric compound preparations, and provides efficient active ingredients for liver protection drugs. The 20 (Z)-ginsenoside F4 with efficient activity, excellent safety and environment-friendly preparation characteristics can be widely applied to preparation of liver protection drugs or pharmaceutical compositions, especially drugs or pharmaceutical compositions for treating liver injury caused by chemotherapeutic drugs.

The application discloses a method for increasing the extraction of saccharide components and / or glycoside components, which comprises the following steps: (1) preparing a sugar solution and / or a sugar alcohol solution; (2) soaking raw materials in the sugar solution and / or the sugar alcohol solution; and (3) heating the raw materials in the sugar solution and / or the sugar alcohol solution for 1-3 times, and obtaining an extract by filtration. The above method can significantly increase the extraction rate of saccharide components and / or glycoside components in the raw materials, thereby helping to improve the utilization rate and curative effect of the raw materials.

The present disclosure provides compositions and methods for producing transgenic plants and other organisms that exhibit increased production of mogroside compounds, in particular mogroside V, and the mogroside compounds, plants and plant parts so produced.

The application provides a saponin extraction method and relates to the technical field of natural product separation. The method comprises the following steps: extraction, water precipitation, resin adsorption, elution and decolorization treatment, active carbon decolorization and drying. The elution process comprises the following steps: first, washing with 0.1%-0.5% NaOH aqueous solution, then washing with water, finally, resolving with alcohol, and collecting the eluent of alcohol resolution; the volume ratio of the NaOH aqueous solution, water and alcohol is 1:7-8:2-3. The method extracts and separates active substances in the stems and leaves of Gypsophila oldhamiana Miq., and for the first time, saponin Rb1 and saponin Rd are separated and extracted from the stems and leaves of Gypsophila oldhamiana Miq., the total extraction content reaches 19.7%, and important basis is provided for the research on the active components of the stems and leaves of Gypsophila oldhamiana Miq.