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Method for preparing mogroside IV

A technique for mogroside and Mogroside, applied in the field of preparation of Mogroside IV, can solve the problems of restricting the development and application of Mogroside IV, high cost, difficult preparation of glycoside IV, etc., and achieves the effects of low cost, simple process and broad application prospect.

Inactive Publication Date: 2012-05-30
成都普瑞法科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the bottleneck problem restricting the application of mogroside IV is the preparation of mogroside IV. The content of mogroside IV in the raw material powder of Luo Han Guo is less than 0.05% (the content of mogroside V is about 2%), so the preparation of mogroside IV is difficult and the cost is high
Seriously restricting the development and application of mogroside IV

Method used

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  • Method for preparing mogroside IV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Preparation of mogroside IV by the method of the present invention

[0068] Crumble 1000kg of fresh Luo Han Guo and put it into the extraction tank, use 4000kg of purified water to extract 3 times in a slightly boiling state, the extraction time is 1.5h, 1h, 1h, the filter residue after each filtration enters the next extraction, and the combined filtrate is concentrated to 4000L . The content of mogroside V was measured to be 0.27% of the fresh weight of Luo Han Guo, and the content of mogroside IV was less than 0.01%. When the temperature was lowered to 50°C, 10 g of β-glucanase (produced by Hersbit Biotechnology Co., Ltd., the same below) was added to adjust the pH to 5, and the hydrolysis time was 20 h. The measured mogroside V content was 0.10%, and the mogroside IV content was 0.12%. Suction filtration, the filtrate was concentrated to a certain volume, and 10% Ca(OH) was added 2 Precipitate impurities, statically prepare, filter, extract the ester-so...

Embodiment 2

[0069] Example 2 Preparation of mogroside IV by the method of the present invention

[0070] Put 10kg of Luo Han Guo dry powder into the extraction tank, reflux extraction with 50L 50% ethanol for 3 times, each extraction time is 2h, and the filter residue after each filtration enters the next extraction, concentrate and dry extract 1kg, and detect Mogroside V by HPLC The content is 8.4%, and the content of mogroside IV is less than 0.1%. Redissolve the extract in 5L of water, filter out the insoluble matter, add 10g of 1,4-β-D-glucan glucoside hydrolase when the temperature rises to 50°C, adjust the pH to 4.8, hydrolyze for 20 hours, and measure the content of mogroside V is 3.4%, and the mogroside IV content is 4.2%. Purified by the scheme of Example 1, 0.17 kg of mogroside IV was obtained, the content of which was above 98%.

Embodiment 3

[0071] Example 3 Preparation of mogroside IV by the method of the present invention

[0072] Put 1kg of 40% mogroside V raw material into the extraction tank, dissolve it with 5L of water, add 10g of β-glucanase when the temperature rises to 50°C, adjust the pH to 5, hydrolyze for 16 hours, and measure the content of mogroside V to be 15.5%. The mogroside IV content is 17.3%. Concentrated, repeatedly purified by preparative HPLC, concentrated, and dried to obtain 0.157 kg of mogroside IV, with a content of more than 98%. HPLC chart of mogroside IV content in Luo Han Guo extract before and after hydrolysis figure 1 shown, from figure 1 It can be seen that the content of mogroside IV after hydrolysis is significantly higher.

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Abstract

The invention which relates to a method for preparing mogroside IV belongs to the traditional Chinese medicinal field. A technical problem to be solved in the invention is to provide a low cost method for preparing the mogroside IV. The method for preparing the mogroside IV in the invention is characterized in that mogroside V, Momordica grosvenori, or a Momordica grosvenori extract containing the mogroside V is hydrolyzed with beta-glucosidase. The method of the invention, which has the advantages of simple technology and low cost, allows the content of the mogroside IV in Momordica grosvenori powder to be improved to above 1.5% from below 0.05%; and the method allows the content of the mogroside IV in the Momordica grosvenori extract to be improved to above 20%, wherein the content of the mogroside V in the Momordica grosvenori extract is 40%, and the original content of the mogroside IV in the Momordica grosvenori extract is 1.5%. The purity of the mogroside IV can reach above 98% after separation and purification. The method of the invention, which provides a new approach for the preparation of the mogroside IV, has a wide application prospect.

Description

technical field [0001] The invention relates to a method for preparing mogroside IV, which belongs to the field of traditional Chinese medicine. Background technique [0002] Luo Han Guo is mainly produced in Guangxi, Jiangxi, and Guangdong. It is also known as monk fruit. It has been planted in my country for hundreds of years. Its application in traditional Chinese medicine can be traced back to 1813. The cucurbitane triterpene glycosides in Luo Han Guo include simonoside I, mogroside II, mogroside III, mogroside IIIE, mogroside IV, mogroside V, etc. Among them, the content of mogroside IV is extremely low, and the extraction is difficult and costly. There are no relevant reports on the preparation process of mogroside IV at home and abroad. [0003] At present, foreign medical workers have found that mogroside IV has a good effect on the treatment of hepatitis C, and it can have a good therapeutic effect alone or in combination with other drugs. Mogroside IV has low tox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/56
CPCC12P33/00C12P19/56C12Y302/01021C07J17/00C07J17/005
Inventor 谢海峰胡云岭张良蕾
Owner 成都普瑞法科技开发有限公司
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