Separation and purification method of high-purity mogroside V

A technology for the separation and purification of mogroside, applied in chemical instruments and methods, glycoside steroids, organic chemistry, etc., can solve the problems of long operation time, complicated operation process, low transfer rate, etc., achieve strong operability, simplify the process flow, low cost effect

Active Publication Date: 2017-01-04
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the purification effect of normal phase silica gel chromatography plus reversed phase C18 column series is relatively ideal, the final transfer rate is very low, only about 50%, and the operation process is complicated, and the distillate needs to be divided into small parts, such as each received A small cup of 20mL requires frequent HPLC testing, high equipment investment and small scale, which is not conducive to actual production and application
[0008] CN 102659739 A discloses a method for extracting chrysin dimethyl ether, which belongs to the technical field of plant active ingredient extraction and separation. The sample is mixed with silica gel and put on a silica gel chromatography column. However, the sample mixed with silica gel is a common method in the laboratory, and an organic solvent is used. Mixing the sample, then evaporating to dry and then loading the column, there are technical defects such as long operation time, small amount, potential safety hazard, and not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Dissolving and centrifuging: Take 100g of Luo Han Guo crude product, add 1kg of deionized water to dissolve, and perform tube centrifugation at a centrifugal rate of 10000r / min to obtain a centrifuge;

[0034] (2) Ultrafiltration, nanofiltration, and concentration: use the ultrafiltration membrane with a molecular weight cut-off of 10,000 Daltons for the centrifugate obtained in step (1), and perform ultrafiltration under the conditions of a membrane pressure of 1.0 MPa and a temperature of 10°C. Until the conductivity of the filtrate is 500μs / cm, the obtained ultrafiltrate is then concentrated into the feed liquid by nanofiltration with a molecular weight cut-off of 1000 Daltons. The pressure on the membrane is 2.0MPa and the temperature is 10°C. The mass concentration of solids is 10%, and the conductivity of the filtrate is up to 500 μs / cm. The obtained nanofiltration retentate is vacuum-concentrated at a vacuum degree of -0.065MPa and a temperature of 88°C until ...

Embodiment 2

[0039] (1) Dissolving and centrifuging: Take 100g of Luo Han Guo crude product, add 1.5kg of deionized water to dissolve, and perform tube centrifugation at a centrifugation rate of 13000r / min to obtain a centrifuge;

[0040] (2) Ultrafiltration, nanofiltration, and concentration: use the ultrafiltration membrane with a molecular weight cut-off of 20,000 Daltons for the centrifugate obtained in step (1), and perform ultrafiltration under the conditions of a membrane pressure of 1.5MPa and a temperature of 15°C. Until the conductivity of the filtrate is 400μs / cm, the obtained ultrafiltrate is then concentrated into the feed liquid with a nanofiltration membrane with a molecular weight cut-off of 3000 Daltons. The pressure on the membrane is 2.6MPa and the temperature is 15°C. The mass concentration of solids is 20%, and the conductivity of the filtrate is up to 400 μs / cm. The obtained nanofiltration retentate is vacuum-concentrated at a vacuum degree of -0.08MPa and a temperatur...

Embodiment 3

[0045] (1) Dissolving and centrifuging: Take 100g of Luo Han Guo crude product, add 2.0kg of deionized water to dissolve, and perform tube centrifugation at a centrifugation rate of 15000r / min to obtain a centrifuge;

[0046] (2) Ultrafiltration, nanofiltration, and concentration: Use an ultrafiltration membrane with a molecular weight cut-off of 30,000 Daltons to filter the centrifugate obtained in step (1) to The conductivity of the filtrate is up to 300μs / cm, and the obtained ultrafiltrate is then concentrated to a nanofiltration membrane with a molecular weight cut-off of 5000 Daltons. The pressure on the membrane is 3MPa and the temperature is 20°C. The mass concentration is 30%, the conductivity of the filtrate is 300μs / cm, and the obtained nanofiltration retentate is vacuum-concentrated at a vacuum degree of -0.1MPa and a temperature of 60°C until the mass concentration of solids in the concentrated solution is 70%. Obtain concentrated solution 110g;

[0047] (3) Silic...

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Abstract

The invention discloses a separation and purification method of high-purity mogroside V. The separation and purification method comprises the following steps: firstly, dissolving and centrifuging: taking a momordicagrosvenori crude product, and adding water for dissolving and centrifuging to obtain centrifugal liquid; secondly, ultrafiltration, nanofiltration and concentration: performing ultrafiltration, nanofiltration and vacuum concentrationon on the centrifugal liquid to obtain a concentrated solution; thirdly, sample mixing of silica gel, eluting and concentrating: performing mixing and sample mixing on the concentrated solution and the silica gel, drying, loading into a silica gel chromatography column, eluting, collecting objective fractions in a segmented manner, and concentrating and drying to obtain mogroside V dry powder; fourthly, crystallizing, washing and drying: dissolving the mogroside V dry powder, filtering, crystallizing, washing a crystal and drying to obtain a high-purity mogroside V product. The purity of the high-purity mogroside V product obtained by the method disclosed by the invention is greater than or equal to 98.4 percent, and a final yield of the high-purity mogroside V product is greater than 90 percent; the method disclosed by the invention breaks limitations of the prior art and is suitable for industrial production; a process flow is simplified; the method has the advantages of high operability, safety, environmental protection and low cost.

Description

technical field [0001] The present invention relates to a method for separation and purification of high-purity mogroside V, in particular to a method for separation and purification of high-purity mogroside V by using the crude product of Luo Han Guo as a raw material. Background technique [0002] Luo Han Guo is sweet in taste and cool in nature, and it belongs to the lung and large intestine meridian. Modern medical research has confirmed that Luo Han Guo contains a sweetener—Mogroside V, which is 300 times sweeter than sucrose, and its calories are only one-fiftieth of sucrose. An ideal alternative sugar source for those who are obese and should not eat sugar. [0003] The purity of mogroside V is an important indicator to measure its sweetness and taste. High-purity mogroside V is currently favored in the food and beverage industries at home and abroad. [0004] Although the large-scale production of Luo Han Guo extract has already been realized in China, the purity o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/00C07J17/005
Inventor 龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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