High-Purity Mogrosides And Process For Their Purification

a mogroside and high-purity technology, applied in the field of high-purity mogroside purification, can solve the problems of affecting the color, flavor, odor and taste profile of the inability to use extracts, and no technology is currently available for purifying low-purity luo han guo extract into high-purity mogrosides

Active Publication Date: 2012-03-08
PURECIRCLE SDN BHD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0030]One objective of the invention to develop an efficient method of preparation of highly purified mogrosides' mixture from low purity mogrosides' mixture.
[0031]Anoth

Problems solved by technology

Such extracts generally cannot be used as a sweetener in foods and beverages as they contain substantial amount of impurities; some of the impurities possess undesirable organoleptic properties,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example i

Purification of High Purity Mogrosides

[0056]100 g of luo ban guo extract (w / w on dry basis) comprising Mogroside V 19.47%, 11-oxo-Mogroside V 5.60%, Siamenoside I 5.20%, and Grosmomoside I 4.46% (TM content 34.73%) was dissolved in 2.4 liters of deionized water. The solution was passed through 6 consecutively connected 200 mL columns packed with Diaion® HP (Mitsubishi Chemical Corp) macroporous adsorbent. 2.4 L deionized water was subsequently passed through the system. Further the columns were separately washed with 2 volumes of 0.5% HCl, then with water until neutral pH, then with 0.5% NaOH and again with water until neutral pH of washing waters is achieved. Then the columns were separately washed with 10% Ethanol. Then the columns were eluted with 50% Ethanol. Columns 4 and 5 were connected consecutively. The eluate of column 4 and 5 was evaporated under vacuum for ethanol removal. Obtained aqueous solution was spray dried to yield 31 g of highly purified mogroside mixture compri...

example 2

Membrane Purification

[0057]100 g of high purity mogrosides prepared according to EXAMPLE 1 comprising Mogroside V 62.82%, 11-oxo-Mogroside V 15.32%, Siamenoside I 5.11%, and Grosmomoside I 3.92% (on dry basis) was dissolved in 2.4 liters of deionized water. The obtained solution was further passed through stirred cell membrane system (Sterlitech Corp., USA). The solution was passed through GE Osmonics 2500 MWCO membrane. The permeate which contained the mogrosides was concentrated to 5% solids and further passed through 1000MWCO membrane. The retentate which contained the mogrosides was concentrate and dried to yield 90 g powder containing Mogroside V 67.13%, 11-oxo-Mogroside V 16.31%, Siamenoside I 5.51%, and Grosmomoside I 4.10% (dry basis).

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Abstract

The present invention provides a process for preparation of highly purified mogrosides mixture from low purity mogrosides mixture. The process comprises providing a mixture of low purity mogrosides, dissolving the low purity mogrosides mixture in water or an aqueous alcohol solution to form an initial solution of mogrosides, passing the initial solution through a column system, wherein the column system comprises a plurality of columns, and each column is packed with a sorbent having different affinities to impurities and mogrosides so that one or more columns retains more mogrosides than other columns, washing the columns to remove impurities with an acidic aqueous solution, a basic aqueous solution, and an aqueous alcoholic solution successively, eluting the columns with an aqueous alcohol solution that contains higher alcohol content than the aqueous alcohol solution used in the washing step, wherein the eluate from the columns with high content of mogrosides are combined, and drying the combined eluate to obtain high purity mogrosides with the content of the total mogrosides are more than 70% (w/w). The present invention also provides a sweetener mixture and product comprising high purity mogrosides.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The present application claims the benefit of US provisional application entitled “High-Purity Mogrosides” filed Sep. 3, 2010 having Ser. No. 61 / 379,729.FIELD OF THE INVENTION[0002]The invention relates to a process for purification of high purity mogrosides from Siraitia grosvenori fruit extract, particularly high purity mogrosides mixtures with high mogroside V content, and further to high purity mogrosides and their applications in food and beverage products.DESCRIPTION OF THE RELATED ART[0003]Luo han guo generally refers to a fruit of Siraitia grosvenori, a member of the Cucurbitaceae family, is a plant native to some regions of southern Asia and China. The sweet taste of luo han guo mainly comes from triterpene glycosides generally known as mogrosides or mogrol glycosides. Mogrosides comprise only about 1% of the luo han guo fruit. There are a number of mogrosides identified in luo han guo but generally mogroside V (CAS No: 88901-36-...

Claims

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Application Information

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IPC IPC(8): A23L1/221A23L2/60C07J75/00A61K47/26A61K8/60A24B15/40A23L1/236A23L1/30A23L11/30A23L27/10A23L27/12A23L27/30
CPCA23L1/2366A23V2002/00C07J17/005A61K8/97A61K8/63A61Q19/00A23V2250/254A23V2300/14A23V2300/34A23L27/34A23L27/36A24B13/00A61K8/9789A61K8/9794G01N2030/027A23V2250/24A23L2/60
Inventor MARKOSYAN, AVETIK
Owner PURECIRCLE SDN BHD
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