High-Purity Mogrosides And Process For Their Purification
a mogroside and high-purity technology, applied in the field of high-purity mogroside purification, can solve the problems of affecting the color, flavor, odor and taste profile of the inability to use extracts, and no technology is currently available for purifying low-purity luo han guo extract into high-purity mogrosides
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example i
Purification of High Purity Mogrosides
[0056]100 g of luo ban guo extract (w / w on dry basis) comprising Mogroside V 19.47%, 11-oxo-Mogroside V 5.60%, Siamenoside I 5.20%, and Grosmomoside I 4.46% (TM content 34.73%) was dissolved in 2.4 liters of deionized water. The solution was passed through 6 consecutively connected 200 mL columns packed with Diaion® HP (Mitsubishi Chemical Corp) macroporous adsorbent. 2.4 L deionized water was subsequently passed through the system. Further the columns were separately washed with 2 volumes of 0.5% HCl, then with water until neutral pH, then with 0.5% NaOH and again with water until neutral pH of washing waters is achieved. Then the columns were separately washed with 10% Ethanol. Then the columns were eluted with 50% Ethanol. Columns 4 and 5 were connected consecutively. The eluate of column 4 and 5 was evaporated under vacuum for ethanol removal. Obtained aqueous solution was spray dried to yield 31 g of highly purified mogroside mixture compri...
example 2
Membrane Purification
[0057]100 g of high purity mogrosides prepared according to EXAMPLE 1 comprising Mogroside V 62.82%, 11-oxo-Mogroside V 15.32%, Siamenoside I 5.11%, and Grosmomoside I 3.92% (on dry basis) was dissolved in 2.4 liters of deionized water. The obtained solution was further passed through stirred cell membrane system (Sterlitech Corp., USA). The solution was passed through GE Osmonics 2500 MWCO membrane. The permeate which contained the mogrosides was concentrated to 5% solids and further passed through 1000MWCO membrane. The retentate which contained the mogrosides was concentrate and dried to yield 90 g powder containing Mogroside V 67.13%, 11-oxo-Mogroside V 16.31%, Siamenoside I 5.51%, and Grosmomoside I 4.10% (dry basis).
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