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Method for extracting mogroside V from momordica grosvenori

A technology of Mogroside and Luo Han Guo, which is applied in the direction of chemical instruments and methods, glycoside steroids, steroids, etc., can solve the problems of low resin utilization rate, complicated process, low product purity, etc., and achieve the effect of product safety and high quality

Inactive Publication Date: 2015-04-29
JIANGXI HAIFU BIOENG
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Problems solved by technology

CN101863946A discloses a method for extracting high-content mogroside V from dried grosvenor fruit. The method includes alcohol extraction, concentration, adsorption and separation of macroporous resin, decolorization of ion exchange resin, concentration and drying to obtain the product after crushing the dried grosvenor fruit. The process is simple and suitable for factory production, but the product purity is low and the resin utilization rate is low
CN101690573A discloses a method for producing Mogroside V content greater than 60% of Luo Han Guo extract. The method is to enzymatically hydrolyze, water extract, concentrate, ion exchange resin decolorization, macroporous resin adsorption separation and alumina chromatography after crushing Luo Han Guo, and finally The product is obtained by concentrating and drying. This method can realize the purity of the product sweet glycoside V greater than 60%. Due to multi-step chromatography, the yield of the final product is low.
CN10140266A discloses a method for preparing mogroside V. The method uses the crude extract of Luo Han Guo as a raw material, and the product is obtained through silica gel chromatography, C18 column reverse phase chromatography, concentration and drying, and the product purity can reach more than 70%. The disadvantage is that a large amount of organic solvent is used in the process, the product yield is low, the cost is high, and it is not suitable for industrial production
CN101177444A discloses a method for extracting mogroside from Luo Han Guo. The method comprises crushing the fresh fruit of Luo Han Guo, enzymatic hydrolysis, multistage countercurrent extraction, centrifugation, ultrafiltration, concentration, macroporous resin adsorption separation, ion exchange resin decolorization, C18 Reversed-phase silica gel chromatography, concentrated and dried to obtain the product. The defects of this method are low product yield, low product purity and complex process, which is not conducive to industrial production
CN104086615A discloses a method for preparing mogroside V from Luo Han Guo. The method is to crush the Luo Han Guo, high-pressure treatment, supercritical fluid extraction, HP-20ss resin chromatography, concentration, and drying to obtain the product. The purity of mogroside V in the product is More than 70%, this method has simple process and high purity, but due to the limitation of one-time processing capacity of supercritical fluid extraction equipment, it is not conducive to continuous large-scale production

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  • Method for extracting mogroside V from momordica grosvenori

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Experimental program
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Effect test

Embodiment 1

[0036] s1 Raw material pretreatment: take 1000 kg of fresh Luo Han Guo fruit, remove impurities, wash, and properly crush, preferably without breaking the Luo Han Guo seeds;

[0037] s2 Microwave countercurrent extraction: Use a microwave countercurrent extraction device to extract the crushed Luo Han Guo. The extraction solvent is deionized water. The material is fed forward and the water is reversed. The flow of material and water is reverse continuous dynamic flow, and the extraction temperature is 90 ℃, the extraction time is 70 min, the microwave power is 800w, and the frequency is 6000MHz. The filter residue is forced to be pushed to the slag outlet by the screw conveyor. Mixed and filtered to obtain the extract;

[0038] s3 multi-stage filtration: Centrifuge the extract obtained in step s2 with a horizontal screw centrifuge at a centrifugal speed of 5000 r / min, then pass through a tubular centrifuge at a centrifugal speed of 14000 r / min, and pass the obtained centrifugate...

Embodiment 2

[0044] s1 Raw material pretreatment: take 1000 kg of fresh Luo Han Guo fruit, remove impurities, wash, and properly crush, preferably without breaking the Luo Han Guo seeds;

[0045] s2 Microwave countercurrent extraction: Use a microwave countercurrent extraction device to extract the crushed Luo Han Guo. The extraction solvent is deionized water. The material is fed forward and the water is reversed. The flow of material and water is reverse continuous dynamic flow, and the extraction temperature is 70 ℃, the extraction time is 50 min, the microwave power is 600w, and the frequency is 8000MHz. The filter residue is forced to be pushed to the slag outlet by the screw conveyor. Mixed and filtered to obtain the extract;

[0046] s3 multi-stage filtration: Centrifuge the extract obtained in step s2 with a horizontal screw centrifuge at a centrifugal speed of 5000 r / min, then pass through a tubular centrifuge at a centrifugal speed of 14000 r / min, and pass the obtained centrifuga...

Embodiment 3

[0052] s1 Raw material pretreatment: take 1000 kg of fresh Luo Han Guo fruit, remove impurities, wash, and properly crush, preferably without breaking the Luo Han Guo seeds;

[0053] s2 Microwave countercurrent extraction: Use a microwave countercurrent extraction device to extract the crushed Luo Han Guo. The extraction solvent is deionized water. The material is fed forward and the water is reversed. The flow of material and water is reverse continuous dynamic flow, and the extraction temperature is 85 ℃, the extraction time is 60 min, the microwave power is 700w, and the frequency is 7000MHz. The filter residue is forced to be pushed to the slag outlet by the screw conveyor. Mixed and filtered to obtain the extract;

[0054] s3 multi-stage filtration: Centrifuge the extract obtained in step s2 with a horizontal screw centrifuge at a centrifugal speed of 5000 r / min, then pass through a tubular centrifuge at a centrifugal speed of 14000 r / min, and pass the obtained centrifuga...

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Abstract

The invention provides a method for extracting mogroside V from momordica grosvenori. The method comprises the steps of carrying out pretreatment on raw materials, carrying out microwave countercurrent extraction, carrying out multistage filtering, carrying out macroreticular resin adsorption separation, carrying out nanofiltration, concentrating and drying. The method integrates the advantages of multiple extracting ways, and is used for continuously and efficiently extracting the mogroside V. Products obtained by adopting the method are proved that the mogroside V is 63-65% and the final yield of the mogroside V is 70-74% after being detected by adopting a high performance liquid chromatography. Except ethyl alcohol, in the method, other organic solvents which are harmful to the human body are not used, thus the products are environmentally friendly; the products contain less than 10ppm of ethyl alcohol residue, thus being safe and high in quality.

Description

technical field [0001] The invention relates to a method for extracting plant components, in particular to a method for extracting mogroside V from Luo Han Guo, which can continuously and efficiently extract mogroside V. Background technique [0002] As a unique plant in my country, Luo Han Guo provides convenience for the research of Luo Han Guo in various aspects due to its geographical advantages. In addition to the compounding of Luo Han Guo, the research mainly focuses on the extraction and purification process of mogroside V. CN101863946A discloses a method for extracting high-content mogroside V from dried grosvenor fruit. The method includes alcohol extraction, concentration, adsorption and separation of macroporous resin, decolorization of ion exchange resin, concentration and drying to obtain the product after crushing the dried grosvenor fruit. The process is simple and suitable for factory production, but the purity of the product is low and the utilization rate ...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 唐方华黄华学
Owner JIANGXI HAIFU BIOENG
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