Method for extracting mogroside V from momordica grosvenori

A technology of Mogroside and Luo Han Guo, which is applied in the direction of chemical instruments and methods, glycoside steroids, steroids, etc., can solve the problems of low resin utilization rate, complicated process, low product purity, etc., and achieve the effect of product safety and high quality

Inactive Publication Date: 2015-04-29
JIANGXI HAIFU BIOENG
View PDF8 Cites 27 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN101863946A discloses a method for extracting high-content mogroside V from dried grosvenor fruit. The method includes alcohol extraction, concentration, adsorption and separation of macroporous resin, decolorization of ion exchange resin, concentration and drying to obtain the product after crushing the dried grosvenor fruit. The process is simple and suitable for factory production, but the product purity is low and the resin utilization rate is low
CN101690573A discloses a method for producing Mogroside V content greater than 60% of Luo Han Guo extract. The method is to enzymatically hydrolyze, water extract, concentrate, ion exchange resin decolorization, macroporous resin adsorption separation and alumina chromatography after crushing Luo Han Guo, and finally The product is obtained by concentrating and drying. This method can realize the purity of the product sweet glycoside V greater than 60%. Due to multi-step chromatography, the yield of the final product is low.
CN10140266A discloses a method for preparing mogroside V. The method uses the crude extract of Luo Han Guo as a raw material, and the product is obtained through silica gel chromatography, C18 column reverse phase chromatography, concentration and drying, and the product purity ca

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting mogroside V from momordica grosvenori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] s1 Raw material pretreatment: take 1000 kg of fresh Luo Han Guo fruit, remove impurities, wash, and properly crush, preferably without breaking the Luo Han Guo seeds;

[0037] s2 Microwave countercurrent extraction: Use a microwave countercurrent extraction device to extract the crushed Luo Han Guo. The extraction solvent is deionized water. The material is fed forward and the water is reversed. The flow of material and water is reverse continuous dynamic flow, and the extraction temperature is 90 ℃, the extraction time is 70 min, the microwave power is 800w, and the frequency is 6000MHz. The filter residue is forced to be pushed to the slag outlet by the screw conveyor. Mixed and filtered to obtain the extract;

[0038] s3 multi-stage filtration: Centrifuge the extract obtained in step s2 with a horizontal screw centrifuge at a centrifugal speed of 5000 r / min, then pass through a tubular centrifuge at a centrifugal speed of 14000 r / min, and pass the obtained centrifugate...

Embodiment 2

[0044] s1 Raw material pretreatment: take 1000 kg of fresh Luo Han Guo fruit, remove impurities, wash, and properly crush, preferably without breaking the Luo Han Guo seeds;

[0045] s2 Microwave countercurrent extraction: Use a microwave countercurrent extraction device to extract the crushed Luo Han Guo. The extraction solvent is deionized water. The material is fed forward and the water is reversed. The flow of material and water is reverse continuous dynamic flow, and the extraction temperature is 70 ℃, the extraction time is 50 min, the microwave power is 600w, and the frequency is 8000MHz. The filter residue is forced to be pushed to the slag outlet by the screw conveyor. Mixed and filtered to obtain the extract;

[0046] s3 multi-stage filtration: Centrifuge the extract obtained in step s2 with a horizontal screw centrifuge at a centrifugal speed of 5000 r / min, then pass through a tubular centrifuge at a centrifugal speed of 14000 r / min, and pass the obtained centrifuga...

Embodiment 3

[0052] s1 Raw material pretreatment: take 1000 kg of fresh Luo Han Guo fruit, remove impurities, wash, and properly crush, preferably without breaking the Luo Han Guo seeds;

[0053] s2 Microwave countercurrent extraction: Use a microwave countercurrent extraction device to extract the crushed Luo Han Guo. The extraction solvent is deionized water. The material is fed forward and the water is reversed. The flow of material and water is reverse continuous dynamic flow, and the extraction temperature is 85 ℃, the extraction time is 60 min, the microwave power is 700w, and the frequency is 7000MHz. The filter residue is forced to be pushed to the slag outlet by the screw conveyor. Mixed and filtered to obtain the extract;

[0054] s3 multi-stage filtration: Centrifuge the extract obtained in step s2 with a horizontal screw centrifuge at a centrifugal speed of 5000 r / min, then pass through a tubular centrifuge at a centrifugal speed of 14000 r / min, and pass the obtained centrifuga...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for extracting mogroside V from momordica grosvenori. The method comprises the steps of carrying out pretreatment on raw materials, carrying out microwave countercurrent extraction, carrying out multistage filtering, carrying out macroreticular resin adsorption separation, carrying out nanofiltration, concentrating and drying. The method integrates the advantages of multiple extracting ways, and is used for continuously and efficiently extracting the mogroside V. Products obtained by adopting the method are proved that the mogroside V is 63-65% and the final yield of the mogroside V is 70-74% after being detected by adopting a high performance liquid chromatography. Except ethyl alcohol, in the method, other organic solvents which are harmful to the human body are not used, thus the products are environmentally friendly; the products contain less than 10ppm of ethyl alcohol residue, thus being safe and high in quality.

Description

technical field [0001] The invention relates to a method for extracting plant components, in particular to a method for extracting mogroside V from Luo Han Guo, which can continuously and efficiently extract mogroside V. Background technique [0002] As a unique plant in my country, Luo Han Guo provides convenience for the research of Luo Han Guo in various aspects due to its geographical advantages. In addition to the compounding of Luo Han Guo, the research mainly focuses on the extraction and purification process of mogroside V. CN101863946A discloses a method for extracting high-content mogroside V from dried grosvenor fruit. The method includes alcohol extraction, concentration, adsorption and separation of macroporous resin, decolorization of ion exchange resin, concentration and drying to obtain the product after crushing the dried grosvenor fruit. The process is simple and suitable for factory production, but the purity of the product is low and the utilization rate ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 唐方华黄华学
Owner JIANGXI HAIFU BIOENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap