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Extraction process of ginseng saponin Rd

A ginsenoside and extraction process technology, applied in the field of extraction process of ginsenoside Rd, can solve the problems of complicated operation, low separation purity, unsuitable for industrial production and the like

Active Publication Date: 2015-05-13
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technology of existing separation and extraction ginsenoside Rd mainly adopts such as figure 2 and image 3 In the method shown, the eluent adopts chloroform-methanol-water system, the extraction process introduces toxic reagent chloroform, and adopts gradient elution, so that the separation purity is not high, the operation is cumbersome, the production process is difficult to control, and it is not suitable for industrial production

Method used

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  • Extraction process of ginseng saponin Rd
  • Extraction process of ginseng saponin Rd
  • Extraction process of ginseng saponin Rd

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 of the present invention: a kind of extraction process of ginsenoside Rd, such as figure 1 shown, including the following steps:

[0059] 1. Grind medicinal materials containing ginsenoside Rd (such as notoginseng, ginseng, American ginseng, etc.) into 50-mesh medicinal powder, add 2 times the amount of 85% ethanol, cold soak for 16 hours, filter, add 1.8 times the amount of 85% ethanol to the medicinal residue , reflux extraction 3 times, 2 hours each time, filter, combine the filtrates, recover ethanol and concentrate to extract with a relative density of 1.24 to obtain the total extract.

[0060] 2. Extraction of total saponins

[0061] (1) Column packing: fully wet the pre-treated D101 macroporous adsorption resin with ethanol, stir to remove air bubbles, pack the column, soak for 12 hours, wash with 95% ethanol until the effluent is mixed with water and does not become turbid, then use purified water Replace the ethanol in it until there is no alcoh...

Embodiment 2

[0073] Embodiment 2 of the present invention: a kind of extraction process of ginsenoside Rd, such as figure 1 shown, including the following steps:

[0074] 1. Grind medicinal materials containing ginsenoside Rd (such as notoginseng, ginseng, American ginseng, etc.) into 40-mesh medicinal powder, add 2 times the amount of 70% ethanol, cold soak for 15 hours, filter, add 1.8 times the amount of 70% ethanol to the medicinal residue , reflux extraction 3 times, 2 hours each time, filter, combine the filtrates, recover ethanol and concentrate to extract with a relative density of 1.15 to obtain the total extract.

[0075] 2. Extraction of total saponins

[0076] (1) Column packing: fully wet the pre-treated D101 macroporous adsorption resin with ethanol, stir to remove air bubbles, pack the column, soak for 12 hours, wash with 95% ethanol until the effluent is mixed with water and does not become turbid, then use purified water Replace the ethanol in it until there is no alcoh...

Embodiment 3

[0088] Embodiment 3 of the present invention: a kind of extraction process of ginsenoside Rd, such as figure 1 shown, including the following steps:

[0089] 1. Grind medicinal materials containing ginsenoside Rd (such as notoginseng, ginseng, American ginseng, etc.) into 60-mesh medicinal powder, add 2 times the amount of 95% ethanol, cold soak for 18 hours, filter, add 1.8 times the amount of 95% ethanol to the medicinal residue , reflux extraction 3 times, 2 hours each time, filter, combine the filtrates, recover ethanol and concentrate to extract with a relative density of 1.26 to obtain the total extract.

[0090] 2. Extraction of total saponins

[0091] (1) Column packing: fully wet the pre-treated D101 macroporous adsorption resin with ethanol, stir to remove air bubbles, pack the column, soak for 12 hours, wash with 95% ethanol until the effluent is mixed with water and does not become turbid, then use purified water Replace the ethanol in it until there is no alcoh...

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Abstract

The invention discloses an extraction process of ginseng saponin Rd. The extraction process comprises the following steps: S1, grinding ginseng saponin Rd-containing herbal materials into herbal powder, performing reflux extraction by using 70-95% ethanol, recycling the ethanol, and concentrating to obtain a total extract; S2, taking the total extract, enabling the total extract to pass through D101 macroporous adsorption resin, eluting by using 40-80% ethanol, recycling the ethanol, and performing deconperssion concentration to obtain total saponin; S3, taking the total saponin, enabling the total saponin to pass through a silica gel chromatography column, performing isocratic elution by using a mixture of n-butanol, ethyl acetate and water, performing thin layer chromatography, combining fractions to obtain crude ginseng saponin Rd; S4, taking the crude ginseng saponin Rd, enabling the crude ginseng saponin Rd to pass through an Rp-C18 silica gel column, performing isocratic elution by using a mixture of methanol and water, performing thin layer chromatography, combining required components, separating out precipitate to obtain the ginseng saponin Rd. By the extraction process, the ginseng saponin Rd separating and purifying effects can be improved, use of a toxic organic reagent is avoided, the operation is simple and convenient and a production process is easy to control; therefore, the extraction process is suitable for industrial production.

Description

technical field [0001] The invention relates to a process for extracting ginsenoside Rd, which belongs to the technical field of ginsenoside extraction. Background technique [0002] Ginsenoside (Ginsenoside) is a sterol compound, triterpene saponin, mainly found in Panax genus medicinal materials. Ginsenosides are regarded as the active ingredients in ginseng, and thus become the target of research. More than 40 kinds of ginsenoside monomers have been isolated and identified. Because ginsenosides affect multiple metabolic pathways, their efficacy is also complex, and the efficacy of various ginsenosides is difficult to separate. However, it is easy to clarify its exact pharmacological effects by conducting biological activity studies on monomers, so there are more and more studies on ginsenoside monomer compounds. Rg in ginsenoside monomer compound 1 , Rb 1 , Rc are relatively high, while Rd, Rg 3 、Rh 2 etc. are rare saponins, but their pharmacological activity is bet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 张观福
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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