38 results about "Thin layer chromatogram" patented technology

The invention discloses a tea oil adulteration detection method based on thin-layer chromatography and image analysis, and belongs to the technical field of tea oil adulteration detection.The tea oil adulteration detection method comprises the steps that firstly, tea oil with different adulteration concentrations is analyzed based on thin-layer chromatography, oil sample images are obtained, and therefore an image recognition database is established; the adulterated tea oil lipid and lipid adjunct with different concentrations have different color development positions under the action of the composite organic solvent, so that the adulterated tea oil machine identification model is constructed by taking different color development positions as characteristic indexes. And identifying a thin-layer chromatogram of a to-be-detected oil sample by adopting the tea oil machine identification model, and judging the adulteration condition of the tea oil according to an identification result. According to the method, the thin-layer chromatography analysis and the machine algorithm are combined, and the thin-layer chromatogram of the sample to be detected is identified and distinguished by utilizing the machine algorithm, so that the tea oil adulteration result and adulteration concentration can be obtained, and the method is simple, convenient and rapid to operate and easy to popularize and promote.

The invention discloses a thin-layer identification method of a nux prinsepiae aqueous extract, the thin-layer identification method comprises the following steps: preparing a reference medicinal material solution and a test solution, the test solution comprises a formula granule test solution and a standard decoction test solution, respectively dropping the reference medicinal material solution and the test solution on the same thin-layer plate, ethyl acetate-methanol-formic acid is used as a developing solvent for developing, a color developing agent is sprayed for color development, and a multi-information nux prinsepiae thin-layer chromatogram is obtained. The method is easy and convenient to operate, good in chromatographic spot separation degree, low in detection cost, environmentally friendly and suitable for identifying the nux prinsepiae aqueous extract.

The present invention provides a thin-layer identification method for pinecone formula granules, comprising: A) taking a test sample, and after pretreatment, obtaining a test solution; taking a pinosylvin monomethyl ether reference substance, and after pretreatment, obtaining a reference solution; B) taking a reference medicinal material of pinecone, and after pretreatment, obtaining a reference medicinal material solution; C) performing thin-layer chromatography detection on the test solution, the reference solution and the reference medicinal material solution, wherein the thin-layer plate is a silica gel G thin-layer plate; the developing agent is toluene-ethyl acetate-glacial acetic acid; D) examining, in the test chromatogram, if spots of the same color appear at the positions corresponding to the reference medicinal material and the reference substance, it is pinecone formula granules. The thin-layer identification chromatographic method for pinecone formula granules established by the present invention uses, as the identification points, the spots of the same color that appear at the positions corresponding to the reference medicinal material and the reference substance by observing the thin-layer chromatogram, so as to quickly and effectively identify pinecone formula granules.

The application discloses a kind of gardenia and its processed product identification method, comprising: step one, respectively take equal amount of gardenia sample powder, gardenia processed product sample powder;Step two, the above-mentioned powder is respectively soaked with ethanol solution, then ultrasonic extraction is carried out, filtration, filter liquor is evaporated to dryness, obtain gardenia sample crude extract, gardenia processed product sample crude extract;Step three, the above-mentioned crude extract is respectively dissolved with water, then sequentially extracted with petroleum ether, ethyl acetate twice, and the two extraction liquids are combined and evaporated to dryness, obtain extract, the extract is dissolved with methanol, obtain gardenia sample test solution, gardenia processed product sample test solution;Step four, the above-mentioned test solution is respectively spotted on the same silica gel thin layer plate, with developing agent development, dry, with color developing agent color development, and under ultraviolet lamp, view thin layer chromatogram, according to the number, position, color of fluorescence spot on thin layer chromatogram, identify sample variety.

The embodiment of the invention provides a medicine component identification method and related equipment, and belongs to the technical field of medicine identification. The method comprises: acquiring preset drug sample data including positive drug sample data and negative drug sample data, the positive drug sample data including a first thin-layer chromatography, and the negative drug sample data including a second thin-layer chromatography; constructing a model training map set through the first thin-layer chromatography map to train a preset twin neural network model to obtain a first twin neural network model; constructing a model test atlas set through the first thin-layer chromatography atlas and the second thin-layer chromatography atlas, and performing parameter optimization on the model in combination with the model training atlas set to obtain a target neural network model; and inputting the to-be-identified thin-layer chromatogram into the target neural network model for component identification analysis to obtain a component identification result. According to the embodiment of the invention, reference substances and reference medicinal materials are not used as much as possible in medicine identification, and the purposes of economy, greenness and environmental protection are achieved.

The present invention provides a thin-layer chromatography (TLC) identification method for Amomum villosum formula granules, comprising the steps of preparing a test solution, preparing a control medicinal material solution, and performing TLC identification. The test solution and the control medicinal material solution are prepared using a simple and rapid preparation method, and are spotted on the same TLC plate. After development, the TLC plate is inspected under ultraviolet light to obtain a multi-information TLC of the Amomum villosum formula granules. This method uses the control medicinal material as a reference and flavonoids as the main identification component. It has high specificity and can distinguish Amomum villosum of different origins (Amomum villosum and Amomum villosum var. amomi) and identify mixed and counterfeit Amomum villosum (Amomum villosum var. longifolia).

The application discloses a thin-layer identification method of Prinsepia uniflora water extract. The method prepares a control medicinal material solution and a test sample solution, the test sample solution comprises a formula granule test sample solution and a standard decoction test sample solution, the control medicinal material solution and the test sample solution are spotted on the same thin-layer plate, ethyl acetate-methanol-formic acid is used as a developing agent for development, a color developing agent is sprayed for color development, and a multi-information Prinsepia uniflora thin-layer chromatogram is obtained. The method is simple in operation, good in chromatographic spot separation degree, low in detection cost, green and environment-friendly, and suitable for identification of the Prinsepia uniflora water extract.

The present invention provides a thin-layer identification method for the formula granules of Lycopodiastrum casuarinoides and Lycopodium clavatum, comprising: A) taking a sample to be tested, and obtaining a test solution after pretreatment; B) taking the reference medicinal material of Lycopodiastrum casuarinoides, and obtaining a reference medicinal material solution after pretreatment; C) performing thin-layer chromatography detection on the test solution and the reference medicinal material solution, and the thin-layer plate is a silica gel G thin-layer plate; the developing agent is cyclohexane-ethyl acetate; D) visualizing under an ultraviolet lamp. In the chromatogram of the test sample, if there is a blue fluorescent spot at an Rf value of 0.31 to 0.464, it is the formula granules of Lycopodiastrum casuarinoides; if there is no blue fluorescent spot at an Rf value of 0.31 to 0.464, it is the formula granules of Lycopodium clavatum. The thin-layer identification chromatographic method for the formula granules of Lycopodiastrum casuarinoides and Lycopodium clavatum established by the present invention uses the presence or absence of fluorescent spots within a specified retention factor value range in the thin-layer chromatogram as the identification point to quickly and effectively identify the formula granules of Lycopodiastrum casuarinoides and Lycopodium clavatum.

The present invention provides a thin-layer identification chromatographic method for Celosiae Semen and stir-fried Celosiae Semen formula granules, including: spotting the test solution on a silica gel G thin-layer plate, developing under thin-layer chromatographic conditions, and inspecting; the thin-layer chromatographic conditions: using toluene-ethyl acetate-methanol as the developing agent. By comparing the thin-layer chromatogram of the stir-fried Celosiae Semen formula granules with that of the Celosiae Semen control medicinal material and the Celosiae Semen granules, the same position with a retention factor value at 0.55-0.67 should not show fluorescent spots, which is used as the identification point 1 of the stir-fried Celosiae Semen granules; by comparing the thin-layer chromatogram of the stir-fried Celosiae Semen formula granules with that of the Celosiae Semen control medicinal material and the Celosiae Semen granules, the same position with a retention factor value at 0.43-0.52 has one more fluorescent spot, which is used as the identification point 2 of the stir-fried Celosiae Semen granules. This method can quickly and effectively identify the stir-fried Celosiae Semen formula granules that have lost the form of the cut crude drug, with simple operation, high precision, high sensitivity, good stability, and has good application prospects.

The utility model discloses a light source adjusting device of a thin-layer chromatography scanner, and relates to the technical field of thin-layer chromatography scanners. The scanner comprises a scanner main body, a scanning table, a circuit board and a halogen tungsten lamp, the circuit board is installed inside the scanner main body, the halogen tungsten lamp is installed on a lamp holder of the circuit board, and a light source mechanism is arranged inside the scanner main body. The first gear drives the worm to rotate, the worm drives the worm gear to rotate, the transmission speed can be further reduced, the worm gear drives the transmission shaft to rotate, the transmission shaft drives the grating to rotate slowly and uniformly through the first rotating shaft, the situation that the grating is difficult to rotate uniformly through manual operation can be avoided, and the purpose of uniform-speed rotation is achieved. The threaded rod rotates to drive the moving block to move, the moving block drives the connecting block to move, and the connecting block drives the focusing lens to move, so that the position of the focusing lens can be adjusted to effectively focus light rays, and the purpose of improving the focusing effect is achieved.

The utility model provides a kind of thin layer chromatogram sample application device, including base, the base is provided with sample application platform, the sample application platform is provided with sample application seat, the one side of the sample application platform is provided with support, the support is provided with sample application head;The base is provided with intermittent drive assembly, the intermittent drive assembly is matched with the sample application platform;The inside of base is provided with support frame, the rotating motor passes through the support frame and is connected with the drive shaft, the drive shaft is connected with the drive gear, one end of the transmission shaft is connected with the support frame, and the other end passes through the transmission gear and is connected with the driving element, the transmission gear is engaged with the drive gear.The thin layer chromatogram sample application device provided by the utility model is provided with intermittent drive assembly, the intermittent rotation of sample application platform is driven by gear engagement driving element, continuous sample application can be realized, and sample application efficiency is improved.

The invention provides a thin layer chromatography identification method for pumpkin stalk medicinal materials and preparations thereof. The method comprises the following steps: taking a sample to be tested, decocting with water, filtering, concentrating, extracting with a first solvent, evaporating to dryness, adding water and hydrochloric acid to the residue for extraction by heating, evaporating to dryness, and dissolving the residue in a second solvent to obtain a test solution; the sample to be tested is the pumpkin stalk medicinal material and the preparation thereof; taking a pumpkin stalk reference medicinal material, decocting with water, filtering, concentrating, extracting with a first solvent, evaporating to dryness, adding water and hydrochloric acid to the residue for extraction by heating, evaporating to dryness, and dissolving the residue in a second solvent to obtain a control medicinal material solution; performing thin layer chromatography on the test solution and the control medicinal material solution, using a silica gel G thin layer plate and n-hexane-ether-glacial acetic acid as a developing solvent; spraying with a phosphomolybdic acid ethanol solution, heating until spots are clear, and inspecting under ultraviolet light, so that fluorescent spots of the same color appear at corresponding positions in the chromatogram of the test sample and the chromatogram of the control medicinal material. The present invention uses the observation of whether the fluorescent spots of the same color appear at the corresponding positions of the thin-layer chromatogram and the chromatogram of the control medicinal material as identification points to quickly and effectively identify the pumpkin pedicle medicinal material and its preparation, thereby ensuring the accuracy of clinical medication.

The method comprises the following steps: grinding the common goldenrop particles, adding ethyl acetate, carrying out ultrasonic treatment, filtering, carrying out rotary evaporation on the filtrate to dryness, and dissolving residues with ethyl acetate to obtain a test solution; taking a 6-gingerol reference substance, and adding methanol to prepare a solution as a reference substance solution; sucking the test sample solution and the reference substance solution, respectively dripping the test sample solution and the reference substance solution on the same silica gel thin-layer plate, developing by taking cyclohexane-trichloromethane-ethyl acetate with the volume ratio of 4: 2: 3 as a developing solvent, taking out, airing, spraying a vanillic aldehyde sulfuric acid solution, heating, and inspecting. The thin-layer chromatogram obtained by the method is uniform in spot distribution, regular in shape, obvious in color contrast, stable in specific shift value and good in reproducibility. In addition, the method is excellent in durability, has good adaptability under different environment temperature and humidity and different types of thin-layer plate operation conditions, and is stable and reliable in separation effect. And a reliable method is provided for quality control of the common goldenrop particles.

The application provides a kind of stone lotus seed extract, bitter stone lotus extract thin layer identification method, comprising: taking stone lotus seed extract pretreatment, to obtain test solution;Take bitter stone lotus extract pretreatment, to obtain bitter stone lotus extract solution;Take stone lotus control medicinal material, and water decoction is obtained control medicinal material solution;Test solution, bitter stone lotus extract solution and control medicinal material solution are detected by thin layer chromatography, and thin layer plate is silica gel G thin layer plate;Developing agent is n-butanol-isopropyl alcohol-water-glacial acetic acid;Under the observation of ultraviolet light, if test solution and control medicinal material solution have the same color fluorescent spot at Rf equal place, while there is no fluorescent spot at Rf=0.64-0.79, it is stone lotus seed extract;If there is fluorescent spot at Rf=0.64-0.79, it is bitter stone lotus extract.The application is used for distinguishing stone lotus seed extract and bitter stone lotus extract by whether there is fluorescent spot in the range of relative migration value in thin layer chromatogram.

The present invention provides a thin-layer identification method for extracts of Canavalia gladiata and Canavalia ensiformis, comprising: pretreating the extract of Canavalia gladiata to obtain a test solution; pretreating the extract of Canavalia ensiformis to obtain an extract solution of Canavalia ensiformis; taking the reference medicinal material of Canavalia gladiata, decocting with water, adding methanol after filtration to obtain a reference medicinal material solution; adding a reference substance to a methanol solution to obtain a reference substance solution; performing thin-layer chromatography detection on the test solution, the extract solution of Canavalia ensiformis, the reference substance solution and the reference medicinal material solution, spraying with ninhydrin solution, heating and examining. If the test solution and the reference medicinal material solution have fluorescent spots of the same color at the same Rf value, it is the extract of Canavalia gladiata. The thin-layer identification chromatographic method for the extract of Canavalia gladiata established by the present invention is used for quickly, accurately and effectively identifying the extract of Canavalia gladiata. At the same time, by observing whether there are fluorescent spots within a certain range of the specific migration value in the thin-layer chromatogram as the identification point, it is used to distinguish the extract of Canavalia gladiata and the extract of Canavalia ensiformis.

The invention discloses a thin-layer chromatography identification method and application of radix cynanchi auriculati traditional Chinese medicine formula granules and radix cynanchi auriculati traditional Chinese medicine formula granules as well as a preparation method of the radix cynanchi auriculati traditional Chinese medicine formula granules and the radix cynanchi auriculati traditional Chinese medicine formula granules, and relates to the technical field of detection methods. The method comprises the following steps: dripping a test solution on a silica gel G thin-layer plate, and developing and inspecting under thin-layer chromatography conditions; thin-layer chromatography conditions are as follows: n-hexane-ethyl formate-formic acid is used as a developing solvent; the volume ratio of the normal hexane to the ethyl formate to the formic acid is 20: 15: 1; the spreading temperature is 4-26 DEG C, and the spreading humidity is 30% rh to 60% rh; compared with a thin-layer chromatogram of the radix cynanchi auriculati traditional Chinese medicine formula granules, the radix cynanchi auriculati charcoal traditional Chinese medicine formula granules have fluorescent spots at the specific shift value of 0.421-0.458. The method can be used for rapidly and effectively identifying the radix cynanchi bungei traditional Chinese medicine formula granules or radix cynanchi bungei charcoal traditional Chinese medicine formula granules which lose the form of the decoction pieces, is simple and convenient to operate, high in precision and sensitivity and good in stability, and has a good application prospect.

The present invention provides a thin layer chromatography identification method for scutellaria baicalensis formula granules and scutellaria baicalensis charcoal formula granules, comprising the following steps: S1) pre-treating a sample to be tested to obtain a test solution; the sample to be tested is scutellaria baicalensis formula granules and scutellaria baicalensis charcoal formula granules; pre-treating a reference substance to obtain a reference substance solution; pre-treating a scutellaria baicalensis reference medicinal material to obtain a reference medicinal material solution; S2) performing thin layer chromatography on the test solution, the reference solution and the reference medicinal material solution, using a silica gel GF thin layer plate. 254 The thin-layer chromatography identification method for Huangqin formula granules and Huangqin charcoal formula granules established in the present invention uses the presence of spots within a specified ratio shift range on the thin-layer chromatography spectrum as identification points to quickly and effectively identify Huangqin formula granules and Huangqin charcoal formula granules, thereby ensuring the accuracy of clinical medication.

The invention provides a thin layer chromatography identification method for Radix Dichroae formula granules, comprising: taking a sample to be tested, dissolving it in a solvent, and obtaining a test solution; the sample to be tested is Smilax china formula granules or Smilax china longituba formula granules; taking a resveratrol reference substance, adding a solvent and dissolving it, and obtaining a reference substance solution; taking a Smilax china longituba reference medicinal material, dissolving it in a solvent, and obtaining a control medicinal material solution; performing thin layer chromatography on the test solution, the reference substance solution, and the control medicinal material solution, using a silica gel G thin layer plate and a developing solvent of toluene-ethyl acetate-formic acid; and inspecting under ultraviolet light, and if a blue fluorescent spot is present at an Rf value of 0.829±10% in the chromatogram of the test sample, the sample is Smilax china longituba; and if no blue fluorescent spot is present at an Rf value of 0.829±10%, the sample is Smilax china longituba. The method established by the present invention can effectively identify the Radix Dichroae formula granules by observing whether there are fluorescent spots in the thin layer chromatographic spectrum within a specified ratio shift value range.

The embodiment of the application provides a drug component identification method and related equipment, and belongs to the technical field of drug identification. The method comprises the following steps: obtaining preset drug sample data, including positive drug sample data and negative drug sample data, wherein the positive drug sample data comprises a first thin layer chromatogram, and the negative drug sample data comprises a second thin layer chromatogram; constructing a model training atlas set through the first thin layer chromatogram, so as to train a preset twin neural network model, and obtain a first twin neural network model; constructing a model test atlas set through the first thin layer chromatogram and the second thin layer chromatogram, so as to optimize parameters of the model in combination with the model training atlas set, and obtain a target neural network model; and inputting a to-be-identified thin layer chromatogram into the target neural network model for component identification analysis, and obtaining a component identification result. The embodiment of the application can try not to use control samples and control medicinal materials in drug identification, so that the economic, green and environmentally-friendly purposes are achieved.

This invention provides a thin-layer chromatography (TLC) identification method for lotus root nodes and charred lotus root node formulation granules, comprising: A) pretreating lotus root node formulation granules to obtain a lotus root node test solution; pretreating lotus root node charred formulation granules to obtain a lotus root node charred test solution; B) taking lotus root node reference material, extracting by acid reflux, then extracting with chloroform, evaporating to dryness, dissolving the residue in methanol to obtain a reference material solution; C) performing TLC detection on the lotus root node test solution, the lotus root node charred test solution, and the reference material solution; D) examining under ultraviolet light. The TLC identification method for lotus root nodes and charred lotus root node formulation granules established in this invention is used for rapid, accurate, and effective identification of lotus root nodes and charred lotus root node formulation granules. Simultaneously, observing whether there are fluorescent spots in the TLC chromatogram within a certain ratio shift range serves as identification points to distinguish between lotus root nodes and charred lotus root node formulation granules.

The invention discloses a thin-layer identification method of a smoked plum charcoal related sample, which comprises the following steps: preparing a test sample solution of the smoked plum charcoal related sample and a smoked plum reference medicinal material solution, spotting the test sample solution and the smoked plum reference medicinal material solution on the same thin-layer plate, developing by using a butyl acetate-formic acid-water mixed solution as a developing solvent, inspecting under an ultraviolet lamp, a thin-layer chromatogram is obtained, and identification operation is completed. The method is simple, convenient and rapid, is suitable for various smoked plum charcoal related samples, and is also suitable for smoked plum related samples; after expansion, a multi-information dark plum charcoal related sample thin-layer chromatogram can be obtained, spots are clear, the separation degree is good, and dark plum charcoal related samples and common counterfeit products thereof can be well distinguished. Meanwhile, compared with a pharmacopoeia method, the method has the advantages that the steps are simpler and more convenient, the identification efficiency can be effectively improved, and the method is more suitable for identifying the smoked plum charcoal-related water extract preparation.

The invention provides a dark plum charcoal preparation thin-layer identification method which comprises the following steps: A) taking a to-be-detected sample, and dissolving the to-be-detected sample in a solvent to obtain a test solution; the sample to be detected is a dark plum charcoal preparation; b) taking a smoked plum reference medicinal material, and dissolving with a solvent to obtain a reference medicinal material solution; c) performing thin-layer chromatography detection on the test solution and the reference medicinal material solution, wherein a thin-layer plate is a silica gel G thin-layer plate; the developing solvent is ethyl acetate-cyclohexane; and D) inspecting under an ultraviolet lamp, and if fluorescent spots with the same color are shown in the chromatogram of the sample to be tested and in the position corresponding to the chromatogram of the reference medicinal material, determining that the sample is the smoked plum charcoal formula granules. According to the established thin-layer identification chromatography method for the smoked plum charcoal formula granules, whether fluorescent spots exist at corresponding positions of a thin-layer chromatogram or not is observed to serve as identification points, and the smoked plum charcoal formula granules are rapidly and effectively identified.

The present invention relates to the field of quality control of traditional Chinese medicines, and particularly to a thin-layer identification method for Perilla frutescens leaves and its preparations. First, a simple and rapid pretreatment method is used to obtain a test solution and a control crude drug solution, which are respectively spotted on the same polyamide film. A quaternary mixture of aromatic hydrocarbon / ester / alcohol / acid, preferably a quaternary mixture of toluene / ethyl acetate / methanol / formic acid with a volume ratio of (14-18):(3-10):1:(0.1-0.5), is used as the developing agent for development. Visual inspection is carried out under ultraviolet light to obtain a thin-layer chromatogram of the Perilla frutescens leaf sample with multiple information. Compared with the pharmacopoeia and existing literature reports, this method is simple, rapid, with clear chromatographic spots, good resolution, and low detection cost; evaluation is carried out using the consistency of the control crude drug, which is more accurate and scientific.

The application provides an application of a developing agent in a thin-layer identification method of Fructus Aurantii Immaturus, medicinal slices, intermediates and finished products containing Fructus Aurantii Immaturus as raw materials, and the developing agent is selected from a lower solution of a mixed solution of chloroform, methanol, water and toluene with a volume ratio of 19:7:2:2-15:5:1:1. According to the chemical structure and properties of each effective component of traditional Chinese medicines, the extraction principle of similar solubility is followed, and a suitable extraction solvent is adopted to simply and quickly prepare a test sample solution and a control medicinal material solution. Then, a suitable developing agent is adopted for development, and various chemical components can be well separated on a thin-layer plate according to the different adsorption, desorption, re-adsorption and re-desorption capacities of the developing agent. Then, various effective components with similar polarities present different color spots under the same viewing conditions on the same thin-layer plate, and a thin-layer chromatogram is obtained.

The utility model discloses a special thin-layer chromatography developing box for a laboratory, which comprises a developing box, a control panel is arranged at the upper end of the outer surface of the front end of the developing box, a thin-layer plate is arranged in the developing box, and a placing mechanism is arranged at the lower end of the thin-layer plate. The placing mechanism comprises a bracket, an antiskid plate, an exhaust fan, a fixing frame, a first limiting groove, a T-shaped pulling plate, a frame body, a filtering plate, a deodorizing plate, a side plate, a rubber pad, a clamping groove, a clamping plate, a limiting block, a second limiting groove, a spring and a hole groove; and the exhaust fan is located in the middle of the outer surface of the upper end of the color developing box. According to the special thin-layer chromatography developing box for the laboratory disclosed by the utility model, the anti-skid plate can be used for increasing friction, reducing the influence of sliding and falling of the thin-layer plate on placement, conveniently removing a reagent smell and reducing the environmental pollution caused by peculiar smell, acid-resisting, alkali-resisting and corrosion-resisting treatment is carried out on the inner wall of the developing box, the developing box is convenient to clean, and the frame body is convenient to mount, dismount and replace; and a better use prospect is brought.

The thin-layer identification method comprises the following steps: preparing a control decoction piece solution, a decoction piece test solution, and a formula granule and standard decoction test solution, respectively dispensing the control decoction piece solution and the test solution on the same thin-layer plate, developing by using a developing solvent, spraying a color developing agent for color development, and inspecting under the sunlight, so as to obtain the thin-layer identification of the charred schizonepeta and the preparation thereof. And obtaining a thin-layer chromatogram under the sunlight so as to finish the identification operation. The method disclosed by the invention is simple, convenient and rapid to operate, good in chromatographic spot separation degree, low in detection cost, safe and environment-friendly, can distinguish the schizonepeta from the schizonepeta charcoal sample, and can provide reference for quality control of the schizonepeta charcoal.

The invention discloses a thin-layer chromatography identification method of Xipayi gingival fixing liquid and application of the thin-layer chromatography identification method. The thin-layer chromatography identification method comprises the following steps: 1) preparing a reference substance solution; (2) dripping the reference substance solution and the test solution on the same polyamide thin-layer plate, developing by using a developing solvent, and inspecting to obtain a thin-layer chromatogram; (3) in the thin-layer chromatogram, the test solution displays spots with the same color at the spot positions of the reference solution; wherein the test solution is a Xipayi gingival fixing liquid sample; the reference substance solution is selected from any one or more of gallic acid or 1, 3, 6-trigalloylglucose. The method is free of complex sample pretreatment, simple, convenient and rapid to operate, high in specificity and good in reproducibility, and a reliable and efficient identification method is provided for quality control of the Xipayi gingival fixing liquid preparation.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a thin-layer chromatography construction method of angelica sinensis blood replenishing decoction, a method for identifying astragalus membranaceus components and application of the angelica sinensis blood replenishing decoction. According to the method provided by the invention, the angelica sinensis blood replenishing decoction is used as a test solution, the astragalus mongholicus (astragalus mongholicus) is used as a reference medicinal material for thin-layer identification, the astragalus mongholicus (astragalus mongholicus) can be rapidly detected, corresponding spots are clear, the thin-layer chromatographic separation effect is good, the specific shift value is moderate, the reproducibility is good, and negative control is free of interference; the method is high in specificity, simple and convenient and easy to operate. Therefore, the radix astragali (Astragalus mongolicus) in the radix angelicae sinensis blood replenishing decoction can be quickly and accurately detected finally, the accuracy and comprehensiveness of quality control of the radix angelicae sinensis blood replenishing decoction are improved, and overall evaluation of the quality of the radix angelicae sinensis blood replenishing decoction is facilitated.

The invention provides a thin-layer identification chromatography method for a gardenia and fermented soybean soup reference sample. The thin-layer identification chromatography method comprises the following steps: a, preparing a test solution; b, preparing a reference substance solution; c, developing a thin layer, wherein a developing solvent is toluene-ethyl acetate-acetone-formic acid in a volume ratio of 20: 4: 4: 1; and inspecting under an ultraviolet lamp. According to the thin-layer identification chromatography method for the standard sample (fermented soybean) of the classic famous prescription gardenia and fermented soybean soup, the fermented soybean in the classic famous prescription gardenia and fermented soybean soup can be rapidly, accurately and effectively identified. Meanwhile, whether main spots with the same color are displayed at the same position or not is observed as an identification point by comparing a thin-layer chromatogram with a reference substance and a reference medicinal material, and the method is used for rapidly and effectively identifying the fermented soybean in the classic famous formula cape jasmine and fermented soybean soup, so that guidance is provided for quality control of the classic famous formula cape jasmine and fermented soybean soup and related preparations of the classic famous formula cape jasmine and fermented soybean soup.

The present invention provides a thin layer identification method for black sesame and fried black sesame formula granules, including: taking a sample to be tested, dissolving with a solvent to obtain a test solution; taking sesamin and β-sitosterol reference substances, dissolving with a solvent to obtain a reference solution; taking a black sesame reference medicinal material, dissolving with a solvent to obtain a reference medicinal material solution; subjecting the test solution, the reference solution and the reference medicinal material solution to thin layer chromatography, the thin layer plate being a silica gel G thin layer plate; the developing agent being petroleum ether-toluene-chloroform; and inspecting under ultraviolet light, if there is a blue fluorescent spot at an Rf value of 0.561 in the test sample chromatogram, it is a black sesame formula granule; if there is no blue fluorescent spot at an Rf value of 0.582±10%, it is a fried black sesame formula granule. The method of the present invention is to observe whether there is a fluorescent spot in the thin layer chromatogram within a specified ratio shift value range as a distinguishing point for quickly and effectively identifying black sesame and fried black sesame formula granules.