12 results about "Thin layer chromatogram" patented technology

The utility model provides a kind of thin layer chromatogram sample application device, including base, the base is provided with sample application platform, the sample application platform is provided with sample application seat, the one side of the sample application platform is provided with support, the support is provided with sample application head;The base is provided with intermittent drive assembly, the intermittent drive assembly is matched with the sample application platform;The inside of base is provided with support frame, the rotating motor passes through the support frame and is connected with the drive shaft, the drive shaft is connected with the drive gear, one end of the transmission shaft is connected with the support frame, and the other end passes through the transmission gear and is connected with the driving element, the transmission gear is engaged with the drive gear.The thin layer chromatogram sample application device provided by the utility model is provided with intermittent drive assembly, the intermittent rotation of sample application platform is driven by gear engagement driving element, continuous sample application can be realized, and sample application efficiency is improved.

The method comprises the following steps: grinding the common goldenrop particles, adding ethyl acetate, carrying out ultrasonic treatment, filtering, carrying out rotary evaporation on the filtrate to dryness, and dissolving residues with ethyl acetate to obtain a test solution; taking a 6-gingerol reference substance, and adding methanol to prepare a solution as a reference substance solution; sucking the test sample solution and the reference substance solution, respectively dripping the test sample solution and the reference substance solution on the same silica gel thin-layer plate, developing by taking cyclohexane-trichloromethane-ethyl acetate with the volume ratio of 4: 2: 3 as a developing solvent, taking out, airing, spraying a vanillic aldehyde sulfuric acid solution, heating, and inspecting. The thin-layer chromatogram obtained by the method is uniform in spot distribution, regular in shape, obvious in color contrast, stable in specific shift value and good in reproducibility. In addition, the method is excellent in durability, has good adaptability under different environment temperature and humidity and different types of thin-layer plate operation conditions, and is stable and reliable in separation effect. And a reliable method is provided for quality control of the common goldenrop particles.

The invention discloses a thin-layer chromatography identification method and application of radix cynanchi auriculati traditional Chinese medicine formula granules and radix cynanchi auriculati traditional Chinese medicine formula granules as well as a preparation method of the radix cynanchi auriculati traditional Chinese medicine formula granules and the radix cynanchi auriculati traditional Chinese medicine formula granules, and relates to the technical field of detection methods. The method comprises the following steps: dripping a test solution on a silica gel G thin-layer plate, and developing and inspecting under thin-layer chromatography conditions; thin-layer chromatography conditions are as follows: n-hexane-ethyl formate-formic acid is used as a developing solvent; the volume ratio of the normal hexane to the ethyl formate to the formic acid is 20: 15: 1; the spreading temperature is 4-26 DEG C, and the spreading humidity is 30% rh to 60% rh; compared with a thin-layer chromatogram of the radix cynanchi auriculati traditional Chinese medicine formula granules, the radix cynanchi auriculati charcoal traditional Chinese medicine formula granules have fluorescent spots at the specific shift value of 0.421-0.458. The method can be used for rapidly and effectively identifying the radix cynanchi bungei traditional Chinese medicine formula granules or radix cynanchi bungei charcoal traditional Chinese medicine formula granules which lose the form of the decoction pieces, is simple and convenient to operate, high in precision and sensitivity and good in stability, and has a good application prospect.

The embodiment of the application provides a drug component identification method and related equipment, and belongs to the technical field of drug identification. The method comprises the following steps: obtaining preset drug sample data, including positive drug sample data and negative drug sample data, wherein the positive drug sample data comprises a first thin layer chromatogram, and the negative drug sample data comprises a second thin layer chromatogram; constructing a model training atlas set through the first thin layer chromatogram, so as to train a preset twin neural network model, and obtain a first twin neural network model; constructing a model test atlas set through the first thin layer chromatogram and the second thin layer chromatogram, so as to optimize parameters of the model in combination with the model training atlas set, and obtain a target neural network model; and inputting a to-be-identified thin layer chromatogram into the target neural network model for component identification analysis, and obtaining a component identification result. The embodiment of the application can try not to use control samples and control medicinal materials in drug identification, so that the economic, green and environmentally-friendly purposes are achieved.

This invention provides a thin-layer chromatography (TLC) identification method for lotus root nodes and charred lotus root node formulation granules, comprising: A) pretreating lotus root node formulation granules to obtain a lotus root node test solution; pretreating lotus root node charred formulation granules to obtain a lotus root node charred test solution; B) taking lotus root node reference material, extracting by acid reflux, then extracting with chloroform, evaporating to dryness, dissolving the residue in methanol to obtain a reference material solution; C) performing TLC detection on the lotus root node test solution, the lotus root node charred test solution, and the reference material solution; D) examining under ultraviolet light. The TLC identification method for lotus root nodes and charred lotus root node formulation granules established in this invention is used for rapid, accurate, and effective identification of lotus root nodes and charred lotus root node formulation granules. Simultaneously, observing whether there are fluorescent spots in the TLC chromatogram within a certain ratio shift range serves as identification points to distinguish between lotus root nodes and charred lotus root node formulation granules.

The invention discloses a thin-layer identification method of a smoked plum charcoal related sample, which comprises the following steps: preparing a test sample solution of the smoked plum charcoal related sample and a smoked plum reference medicinal material solution, spotting the test sample solution and the smoked plum reference medicinal material solution on the same thin-layer plate, developing by using a butyl acetate-formic acid-water mixed solution as a developing solvent, inspecting under an ultraviolet lamp, a thin-layer chromatogram is obtained, and identification operation is completed. The method is simple, convenient and rapid, is suitable for various smoked plum charcoal related samples, and is also suitable for smoked plum related samples; after expansion, a multi-information dark plum charcoal related sample thin-layer chromatogram can be obtained, spots are clear, the separation degree is good, and dark plum charcoal related samples and common counterfeit products thereof can be well distinguished. Meanwhile, compared with a pharmacopoeia method, the method has the advantages that the steps are simpler and more convenient, the identification efficiency can be effectively improved, and the method is more suitable for identifying the smoked plum charcoal-related water extract preparation.

The utility model discloses a special thin-layer chromatography developing box for a laboratory, which comprises a developing box, a control panel is arranged at the upper end of the outer surface of the front end of the developing box, a thin-layer plate is arranged in the developing box, and a placing mechanism is arranged at the lower end of the thin-layer plate. The placing mechanism comprises a bracket, an antiskid plate, an exhaust fan, a fixing frame, a first limiting groove, a T-shaped pulling plate, a frame body, a filtering plate, a deodorizing plate, a side plate, a rubber pad, a clamping groove, a clamping plate, a limiting block, a second limiting groove, a spring and a hole groove; and the exhaust fan is located in the middle of the outer surface of the upper end of the color developing box. According to the special thin-layer chromatography developing box for the laboratory disclosed by the utility model, the anti-skid plate can be used for increasing friction, reducing the influence of sliding and falling of the thin-layer plate on placement, conveniently removing a reagent smell and reducing the environmental pollution caused by peculiar smell, acid-resisting, alkali-resisting and corrosion-resisting treatment is carried out on the inner wall of the developing box, the developing box is convenient to clean, and the frame body is convenient to mount, dismount and replace; and a better use prospect is brought.

The thin-layer identification method comprises the following steps: preparing a control decoction piece solution, a decoction piece test solution, and a formula granule and standard decoction test solution, respectively dispensing the control decoction piece solution and the test solution on the same thin-layer plate, developing by using a developing solvent, spraying a color developing agent for color development, and inspecting under the sunlight, so as to obtain the thin-layer identification of the charred schizonepeta and the preparation thereof. And obtaining a thin-layer chromatogram under the sunlight so as to finish the identification operation. The method disclosed by the invention is simple, convenient and rapid to operate, good in chromatographic spot separation degree, low in detection cost, safe and environment-friendly, can distinguish the schizonepeta from the schizonepeta charcoal sample, and can provide reference for quality control of the schizonepeta charcoal.

The invention discloses a thin-layer chromatography identification method of Xipayi gingival fixing liquid and application of the thin-layer chromatography identification method. The thin-layer chromatography identification method comprises the following steps: 1) preparing a reference substance solution; (2) dripping the reference substance solution and the test solution on the same polyamide thin-layer plate, developing by using a developing solvent, and inspecting to obtain a thin-layer chromatogram; (3) in the thin-layer chromatogram, the test solution displays spots with the same color at the spot positions of the reference solution; wherein the test solution is a Xipayi gingival fixing liquid sample; the reference substance solution is selected from any one or more of gallic acid or 1, 3, 6-trigalloylglucose. The method is free of complex sample pretreatment, simple, convenient and rapid to operate, high in specificity and good in reproducibility, and a reliable and efficient identification method is provided for quality control of the Xipayi gingival fixing liquid preparation.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a thin-layer chromatography construction method of angelica sinensis blood replenishing decoction, a method for identifying astragalus membranaceus components and application of the angelica sinensis blood replenishing decoction. According to the method provided by the invention, the angelica sinensis blood replenishing decoction is used as a test solution, the astragalus mongholicus (astragalus mongholicus) is used as a reference medicinal material for thin-layer identification, the astragalus mongholicus (astragalus mongholicus) can be rapidly detected, corresponding spots are clear, the thin-layer chromatographic separation effect is good, the specific shift value is moderate, the reproducibility is good, and negative control is free of interference; the method is high in specificity, simple and convenient and easy to operate. Therefore, the radix astragali (Astragalus mongolicus) in the radix angelicae sinensis blood replenishing decoction can be quickly and accurately detected finally, the accuracy and comprehensiveness of quality control of the radix angelicae sinensis blood replenishing decoction are improved, and overall evaluation of the quality of the radix angelicae sinensis blood replenishing decoction is facilitated.

This invention provides a thin-layer chromatography (TLC) identification method for charred plum preparations, comprising: A) dissolving a sample in a solvent to obtain a test solution; the sample being tested is a charred plum preparation; B) dissolving a reference plum material in a solvent to obtain a reference material solution; C) performing TLC detection on the test solution and the reference material solution, using a silica gel G TLC plate and ethyl acetate-cyclohexane as the developing solvent; D) examining under ultraviolet light; if the chromatogram of the test sample shows fluorescent spots of the same color at the corresponding positions as the chromatogram of the reference material, then it is a charred plum preparation granule. The TLC identification method for charred plum preparation granules established in this invention uses the presence of fluorescent spots at corresponding positions in the TLC chromatogram as identification points for rapid and effective identification of charred plum preparation granules.

The invention discloses a capsule quality detection method and a detection system of a Shenqinaokang capsule, which are used for detecting the quality of a Shenqinaokang capsule preparation with multiple components, and relates to the technical field of capsule detection, and the method comprises the following steps: obtaining a sample, and grinding capsule contents to obtain a powder sample; carrying out microscopic feature identification on the powder sample, obtaining a sublimate through trace sublimation, and adding a vanillin sulfuric acid solution for color development so as to collect a microscopic feature image; carrying out thin-layer chromatography identification on the powder sample, preparing a thin-layer test solution, a contrast solution and a negative sample solution, carrying out sample application, developing and developing, then heating, and inspecting and collecting a thin-layer chromatography image under sunlight and ultraviolet light; carrying out high performance liquid chromatography content determination on the powder sample, preparing a liquid phase test sample solution, carrying out sample injection determination, and judging system adaptability parameters; outputting a qualified or unqualified quality judgment conclusion based on a detection result, and storing related detection data for rechecking and tracing; the problem of quality detection of the Shenqinaokang capsule is systematically solved.