24 results about "Thin layer chromatogram" patented technology

The application discloses a kind of gardenia and its processed product identification method, comprising: step one, respectively take equal amount of gardenia sample powder, gardenia processed product sample powder;Step two, the above-mentioned powder is respectively soaked with ethanol solution, then ultrasonic extraction is carried out, filtration, filter liquor is evaporated to dryness, obtain gardenia sample crude extract, gardenia processed product sample crude extract;Step three, the above-mentioned crude extract is respectively dissolved with water, then sequentially extracted with petroleum ether, ethyl acetate twice, and the two extraction liquids are combined and evaporated to dryness, obtain extract, the extract is dissolved with methanol, obtain gardenia sample test solution, gardenia processed product sample test solution;Step four, the above-mentioned test solution is respectively spotted on the same silica gel thin layer plate, with developing agent development, dry, with color developing agent color development, and under ultraviolet lamp, view thin layer chromatogram, according to the number, position, color of fluorescence spot on thin layer chromatogram, identify sample variety.

The embodiment of the invention provides a medicine component identification method and related equipment, and belongs to the technical field of medicine identification. The method comprises: acquiring preset drug sample data including positive drug sample data and negative drug sample data, the positive drug sample data including a first thin-layer chromatography, and the negative drug sample data including a second thin-layer chromatography; constructing a model training map set through the first thin-layer chromatography map to train a preset twin neural network model to obtain a first twin neural network model; constructing a model test atlas set through the first thin-layer chromatography atlas and the second thin-layer chromatography atlas, and performing parameter optimization on the model in combination with the model training atlas set to obtain a target neural network model; and inputting the to-be-identified thin-layer chromatogram into the target neural network model for component identification analysis to obtain a component identification result. According to the embodiment of the invention, reference substances and reference medicinal materials are not used as much as possible in medicine identification, and the purposes of economy, greenness and environmental protection are achieved.

The present invention provides a thin-layer chromatography (TLC) identification method for Amomum villosum formula granules, comprising the steps of preparing a test solution, preparing a control medicinal material solution, and performing TLC identification. The test solution and the control medicinal material solution are prepared using a simple and rapid preparation method, and are spotted on the same TLC plate. After development, the TLC plate is inspected under ultraviolet light to obtain a multi-information TLC of the Amomum villosum formula granules. This method uses the control medicinal material as a reference and flavonoids as the main identification component. It has high specificity and can distinguish Amomum villosum of different origins (Amomum villosum and Amomum villosum var. amomi) and identify mixed and counterfeit Amomum villosum (Amomum villosum var. longifolia).

The application discloses a thin-layer identification method of Prinsepia uniflora water extract. The method prepares a control medicinal material solution and a test sample solution, the test sample solution comprises a formula granule test sample solution and a standard decoction test sample solution, the control medicinal material solution and the test sample solution are spotted on the same thin-layer plate, ethyl acetate-methanol-formic acid is used as a developing agent for development, a color developing agent is sprayed for color development, and a multi-information Prinsepia uniflora thin-layer chromatogram is obtained. The method is simple in operation, good in chromatographic spot separation degree, low in detection cost, green and environment-friendly, and suitable for identification of the Prinsepia uniflora water extract.

The utility model discloses a light source adjusting device of a thin-layer chromatography scanner, and relates to the technical field of thin-layer chromatography scanners. The scanner comprises a scanner main body, a scanning table, a circuit board and a halogen tungsten lamp, the circuit board is installed inside the scanner main body, the halogen tungsten lamp is installed on a lamp holder of the circuit board, and a light source mechanism is arranged inside the scanner main body. The first gear drives the worm to rotate, the worm drives the worm gear to rotate, the transmission speed can be further reduced, the worm gear drives the transmission shaft to rotate, the transmission shaft drives the grating to rotate slowly and uniformly through the first rotating shaft, the situation that the grating is difficult to rotate uniformly through manual operation can be avoided, and the purpose of uniform-speed rotation is achieved. The threaded rod rotates to drive the moving block to move, the moving block drives the connecting block to move, and the connecting block drives the focusing lens to move, so that the position of the focusing lens can be adjusted to effectively focus light rays, and the purpose of improving the focusing effect is achieved.

The utility model provides a kind of thin layer chromatogram sample application device, including base, the base is provided with sample application platform, the sample application platform is provided with sample application seat, the one side of the sample application platform is provided with support, the support is provided with sample application head;The base is provided with intermittent drive assembly, the intermittent drive assembly is matched with the sample application platform;The inside of base is provided with support frame, the rotating motor passes through the support frame and is connected with the drive shaft, the drive shaft is connected with the drive gear, one end of the transmission shaft is connected with the support frame, and the other end passes through the transmission gear and is connected with the driving element, the transmission gear is engaged with the drive gear.The thin layer chromatogram sample application device provided by the utility model is provided with intermittent drive assembly, the intermittent rotation of sample application platform is driven by gear engagement driving element, continuous sample application can be realized, and sample application efficiency is improved.

The method comprises the following steps: grinding the common goldenrop particles, adding ethyl acetate, carrying out ultrasonic treatment, filtering, carrying out rotary evaporation on the filtrate to dryness, and dissolving residues with ethyl acetate to obtain a test solution; taking a 6-gingerol reference substance, and adding methanol to prepare a solution as a reference substance solution; sucking the test sample solution and the reference substance solution, respectively dripping the test sample solution and the reference substance solution on the same silica gel thin-layer plate, developing by taking cyclohexane-trichloromethane-ethyl acetate with the volume ratio of 4: 2: 3 as a developing solvent, taking out, airing, spraying a vanillic aldehyde sulfuric acid solution, heating, and inspecting. The thin-layer chromatogram obtained by the method is uniform in spot distribution, regular in shape, obvious in color contrast, stable in specific shift value and good in reproducibility. In addition, the method is excellent in durability, has good adaptability under different environment temperature and humidity and different types of thin-layer plate operation conditions, and is stable and reliable in separation effect. And a reliable method is provided for quality control of the common goldenrop particles.

The application provides a kind of stone lotus seed extract, bitter stone lotus extract thin layer identification method, comprising: taking stone lotus seed extract pretreatment, to obtain test solution;Take bitter stone lotus extract pretreatment, to obtain bitter stone lotus extract solution;Take stone lotus control medicinal material, and water decoction is obtained control medicinal material solution;Test solution, bitter stone lotus extract solution and control medicinal material solution are detected by thin layer chromatography, and thin layer plate is silica gel G thin layer plate;Developing agent is n-butanol-isopropyl alcohol-water-glacial acetic acid;Under the observation of ultraviolet light, if test solution and control medicinal material solution have the same color fluorescent spot at Rf equal place, while there is no fluorescent spot at Rf=0.64-0.79, it is stone lotus seed extract;If there is fluorescent spot at Rf=0.64-0.79, it is bitter stone lotus extract.The application is used for distinguishing stone lotus seed extract and bitter stone lotus extract by whether there is fluorescent spot in the range of relative migration value in thin layer chromatogram.

The invention discloses a thin-layer chromatography identification method and application of radix cynanchi auriculati traditional Chinese medicine formula granules and radix cynanchi auriculati traditional Chinese medicine formula granules as well as a preparation method of the radix cynanchi auriculati traditional Chinese medicine formula granules and the radix cynanchi auriculati traditional Chinese medicine formula granules, and relates to the technical field of detection methods. The method comprises the following steps: dripping a test solution on a silica gel G thin-layer plate, and developing and inspecting under thin-layer chromatography conditions; thin-layer chromatography conditions are as follows: n-hexane-ethyl formate-formic acid is used as a developing solvent; the volume ratio of the normal hexane to the ethyl formate to the formic acid is 20: 15: 1; the spreading temperature is 4-26 DEG C, and the spreading humidity is 30% rh to 60% rh; compared with a thin-layer chromatogram of the radix cynanchi auriculati traditional Chinese medicine formula granules, the radix cynanchi auriculati charcoal traditional Chinese medicine formula granules have fluorescent spots at the specific shift value of 0.421-0.458. The method can be used for rapidly and effectively identifying the radix cynanchi bungei traditional Chinese medicine formula granules or radix cynanchi bungei charcoal traditional Chinese medicine formula granules which lose the form of the decoction pieces, is simple and convenient to operate, high in precision and sensitivity and good in stability, and has a good application prospect.

The embodiment of the application provides a drug component identification method and related equipment, and belongs to the technical field of drug identification. The method comprises the following steps: obtaining preset drug sample data, including positive drug sample data and negative drug sample data, wherein the positive drug sample data comprises a first thin layer chromatogram, and the negative drug sample data comprises a second thin layer chromatogram; constructing a model training atlas set through the first thin layer chromatogram, so as to train a preset twin neural network model, and obtain a first twin neural network model; constructing a model test atlas set through the first thin layer chromatogram and the second thin layer chromatogram, so as to optimize parameters of the model in combination with the model training atlas set, and obtain a target neural network model; and inputting a to-be-identified thin layer chromatogram into the target neural network model for component identification analysis, and obtaining a component identification result. The embodiment of the application can try not to use control samples and control medicinal materials in drug identification, so that the economic, green and environmentally-friendly purposes are achieved.

This invention provides a thin-layer chromatography (TLC) identification method for lotus root nodes and charred lotus root node formulation granules, comprising: A) pretreating lotus root node formulation granules to obtain a lotus root node test solution; pretreating lotus root node charred formulation granules to obtain a lotus root node charred test solution; B) taking lotus root node reference material, extracting by acid reflux, then extracting with chloroform, evaporating to dryness, dissolving the residue in methanol to obtain a reference material solution; C) performing TLC detection on the lotus root node test solution, the lotus root node charred test solution, and the reference material solution; D) examining under ultraviolet light. The TLC identification method for lotus root nodes and charred lotus root node formulation granules established in this invention is used for rapid, accurate, and effective identification of lotus root nodes and charred lotus root node formulation granules. Simultaneously, observing whether there are fluorescent spots in the TLC chromatogram within a certain ratio shift range serves as identification points to distinguish between lotus root nodes and charred lotus root node formulation granules.

The invention discloses a thin-layer identification method of a smoked plum charcoal related sample, which comprises the following steps: preparing a test sample solution of the smoked plum charcoal related sample and a smoked plum reference medicinal material solution, spotting the test sample solution and the smoked plum reference medicinal material solution on the same thin-layer plate, developing by using a butyl acetate-formic acid-water mixed solution as a developing solvent, inspecting under an ultraviolet lamp, a thin-layer chromatogram is obtained, and identification operation is completed. The method is simple, convenient and rapid, is suitable for various smoked plum charcoal related samples, and is also suitable for smoked plum related samples; after expansion, a multi-information dark plum charcoal related sample thin-layer chromatogram can be obtained, spots are clear, the separation degree is good, and dark plum charcoal related samples and common counterfeit products thereof can be well distinguished. Meanwhile, compared with a pharmacopoeia method, the method has the advantages that the steps are simpler and more convenient, the identification efficiency can be effectively improved, and the method is more suitable for identifying the smoked plum charcoal-related water extract preparation.

The application provides an application of a developing agent in a thin-layer identification method of Fructus Aurantii Immaturus, medicinal slices, intermediates and finished products containing Fructus Aurantii Immaturus as raw materials, and the developing agent is selected from a lower solution of a mixed solution of chloroform, methanol, water and toluene with a volume ratio of 19:7:2:2-15:5:1:1. According to the chemical structure and properties of each effective component of traditional Chinese medicines, the extraction principle of similar solubility is followed, and a suitable extraction solvent is adopted to simply and quickly prepare a test sample solution and a control medicinal material solution. Then, a suitable developing agent is adopted for development, and various chemical components can be well separated on a thin-layer plate according to the different adsorption, desorption, re-adsorption and re-desorption capacities of the developing agent. Then, various effective components with similar polarities present different color spots under the same viewing conditions on the same thin-layer plate, and a thin-layer chromatogram is obtained.

The utility model discloses a special thin-layer chromatography developing box for a laboratory, which comprises a developing box, a control panel is arranged at the upper end of the outer surface of the front end of the developing box, a thin-layer plate is arranged in the developing box, and a placing mechanism is arranged at the lower end of the thin-layer plate. The placing mechanism comprises a bracket, an antiskid plate, an exhaust fan, a fixing frame, a first limiting groove, a T-shaped pulling plate, a frame body, a filtering plate, a deodorizing plate, a side plate, a rubber pad, a clamping groove, a clamping plate, a limiting block, a second limiting groove, a spring and a hole groove; and the exhaust fan is located in the middle of the outer surface of the upper end of the color developing box. According to the special thin-layer chromatography developing box for the laboratory disclosed by the utility model, the anti-skid plate can be used for increasing friction, reducing the influence of sliding and falling of the thin-layer plate on placement, conveniently removing a reagent smell and reducing the environmental pollution caused by peculiar smell, acid-resisting, alkali-resisting and corrosion-resisting treatment is carried out on the inner wall of the developing box, the developing box is convenient to clean, and the frame body is convenient to mount, dismount and replace; and a better use prospect is brought.

The thin-layer identification method comprises the following steps: preparing a control decoction piece solution, a decoction piece test solution, and a formula granule and standard decoction test solution, respectively dispensing the control decoction piece solution and the test solution on the same thin-layer plate, developing by using a developing solvent, spraying a color developing agent for color development, and inspecting under the sunlight, so as to obtain the thin-layer identification of the charred schizonepeta and the preparation thereof. And obtaining a thin-layer chromatogram under the sunlight so as to finish the identification operation. The method disclosed by the invention is simple, convenient and rapid to operate, good in chromatographic spot separation degree, low in detection cost, safe and environment-friendly, can distinguish the schizonepeta from the schizonepeta charcoal sample, and can provide reference for quality control of the schizonepeta charcoal.

The invention discloses a thin-layer chromatography identification method of Xipayi gingival fixing liquid and application of the thin-layer chromatography identification method. The thin-layer chromatography identification method comprises the following steps: 1) preparing a reference substance solution; (2) dripping the reference substance solution and the test solution on the same polyamide thin-layer plate, developing by using a developing solvent, and inspecting to obtain a thin-layer chromatogram; (3) in the thin-layer chromatogram, the test solution displays spots with the same color at the spot positions of the reference solution; wherein the test solution is a Xipayi gingival fixing liquid sample; the reference substance solution is selected from any one or more of gallic acid or 1, 3, 6-trigalloylglucose. The method is free of complex sample pretreatment, simple, convenient and rapid to operate, high in specificity and good in reproducibility, and a reliable and efficient identification method is provided for quality control of the Xipayi gingival fixing liquid preparation.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a thin-layer chromatography construction method of angelica sinensis blood replenishing decoction, a method for identifying astragalus membranaceus components and application of the angelica sinensis blood replenishing decoction. According to the method provided by the invention, the angelica sinensis blood replenishing decoction is used as a test solution, the astragalus mongholicus (astragalus mongholicus) is used as a reference medicinal material for thin-layer identification, the astragalus mongholicus (astragalus mongholicus) can be rapidly detected, corresponding spots are clear, the thin-layer chromatographic separation effect is good, the specific shift value is moderate, the reproducibility is good, and negative control is free of interference; the method is high in specificity, simple and convenient and easy to operate. Therefore, the radix astragali (Astragalus mongolicus) in the radix angelicae sinensis blood replenishing decoction can be quickly and accurately detected finally, the accuracy and comprehensiveness of quality control of the radix angelicae sinensis blood replenishing decoction are improved, and overall evaluation of the quality of the radix angelicae sinensis blood replenishing decoction is facilitated.

The invention provides a thin-layer identification chromatography method for a gardenia and fermented soybean soup reference sample. The thin-layer identification chromatography method comprises the following steps: a, preparing a test solution; b, preparing a reference substance solution; c, developing a thin layer, wherein a developing solvent is toluene-ethyl acetate-acetone-formic acid in a volume ratio of 20: 4: 4: 1; and inspecting under an ultraviolet lamp. According to the thin-layer identification chromatography method for the standard sample (fermented soybean) of the classic famous prescription gardenia and fermented soybean soup, the fermented soybean in the classic famous prescription gardenia and fermented soybean soup can be rapidly, accurately and effectively identified. Meanwhile, whether main spots with the same color are displayed at the same position or not is observed as an identification point by comparing a thin-layer chromatogram with a reference substance and a reference medicinal material, and the method is used for rapidly and effectively identifying the fermented soybean in the classic famous formula cape jasmine and fermented soybean soup, so that guidance is provided for quality control of the classic famous formula cape jasmine and fermented soybean soup and related preparations of the classic famous formula cape jasmine and fermented soybean soup.

The present invention provides a thin layer identification method for black sesame and fried black sesame formula granules, including: taking a sample to be tested, dissolving with a solvent to obtain a test solution; taking sesamin and β-sitosterol reference substances, dissolving with a solvent to obtain a reference solution; taking a black sesame reference medicinal material, dissolving with a solvent to obtain a reference medicinal material solution; subjecting the test solution, the reference solution and the reference medicinal material solution to thin layer chromatography, the thin layer plate being a silica gel G thin layer plate; the developing agent being petroleum ether-toluene-chloroform; and inspecting under ultraviolet light, if there is a blue fluorescent spot at an Rf value of 0.561 in the test sample chromatogram, it is a black sesame formula granule; if there is no blue fluorescent spot at an Rf value of 0.582±10%, it is a fried black sesame formula granule. The method of the present invention is to observe whether there is a fluorescent spot in the thin layer chromatogram within a specified ratio shift value range as a distinguishing point for quickly and effectively identifying black sesame and fried black sesame formula granules.

The present application relates to the technical field of analytical detection, and particularly relates to a thin layer identification method of Shechuan formula granules. The present application provides a thin layer identification method of Shechuan formula granules, comprising: step 1, taking a sample to be detected, obtaining a test solution after pretreatment; taking Shechuan control medicinal materials, obtaining a Shechuan control medicinal material solution after pretreatment; step 2, taking the test solution and the Shechuan control medicinal material solution in step 1 to perform thin layer chromatography detection; the thin layer plate for the thin layer chromatography detection is a silica gel G thin layer plate, and the developing agent is n-butanol-glacial acetic acid-water; step 3, inspection, if there are the same color spots in the test chromatogram corresponding to the position of the Shechuan control medicinal material chromatogram, then the sample is Shechuan formula granules. The thin layer identification method of Shechuan formula granules established by the present application is used for quickly and effectively identifying Shechuan formula granules by observing whether the formula granules and the control medicinal materials have the same color spots at the same position in the thin layer chromatogram.

Provided in the present invention is a thin-layer chromatography for scutellaria baicalensis georgi formula granules and carbonized scutellaria root formula granules, comprising the following steps: S1) pretreating a sample under test, so as to obtain a solution of the sample under test, said sample being scutellaria baicalensis georgi formula granules and carbonized scutellaria root formula granules; separately pretreating reference standards, so as to obtain reference standard solutions; and pretreating a scutellaria baicalensis georgi reference medicinal material so as to obtain a reference medicinal material solution; S2) carrying out a thin-layer chromatography test on the solution of the sample under test, the reference standard solutions and the reference medicinal material solution, a thin-layer plate being a silica gel GF254 thin plate, and a developing agent being chloroform-butyl acetate-anhydrous ethanol-glacial acetic acid; and S3) carrying out inspection and identification under an ultraviolet light. By observing whether there are spots, which are used as identification points, in thin-layer chromatograms within specified retention factor value ranges, the thin-layer chromatography for scutellaria baicalensis georgi formula granules and carbonized scutellaria root formula granules established in the present invention is used for rapidly and effectively identifying scutellaria baicalensis georgi and carbonized scutellaria root formula granules, so as to ensure the accuracy of clinical medication.

This invention provides a thin-layer chromatography (TLC) identification method for charred plum preparations, comprising: A) dissolving a sample in a solvent to obtain a test solution; the sample being tested is a charred plum preparation; B) dissolving a reference plum material in a solvent to obtain a reference material solution; C) performing TLC detection on the test solution and the reference material solution, using a silica gel G TLC plate and ethyl acetate-cyclohexane as the developing solvent; D) examining under ultraviolet light; if the chromatogram of the test sample shows fluorescent spots of the same color at the corresponding positions as the chromatogram of the reference material, then it is a charred plum preparation granule. The TLC identification method for charred plum preparation granules established in this invention uses the presence of fluorescent spots at corresponding positions in the TLC chromatogram as identification points for rapid and effective identification of charred plum preparation granules.

The present invention provides a thin-layer chromatography identification method for Sophora japonica formula granules, the method comprising the steps of preparing a test sample solution, preparing a control medicinal material solution, preparing a reference substance solution, and thin-layer chromatography identification; the test sample solution, the control medicinal material solution, and the reference substance solution are obtained by a simple and quick preparation method, and are spotted on the same thin-layer chromatography plate respectively. After development, the plates are inspected under ultraviolet light to obtain a multi-information thin-layer chromatography diagram of the Sophora japonica formula granules. The method uses the reference medicinal materials and rutin reference substance as reference substances, has strong specificity, is suitable for production practice, and provides a basis for establishing quality control standards for the Sophora japonica formula granules.

The invention discloses a capsule quality detection method and a detection system of a Shenqinaokang capsule, which are used for detecting the quality of a Shenqinaokang capsule preparation with multiple components, and relates to the technical field of capsule detection, and the method comprises the following steps: obtaining a sample, and grinding capsule contents to obtain a powder sample; carrying out microscopic feature identification on the powder sample, obtaining a sublimate through trace sublimation, and adding a vanillin sulfuric acid solution for color development so as to collect a microscopic feature image; carrying out thin-layer chromatography identification on the powder sample, preparing a thin-layer test solution, a contrast solution and a negative sample solution, carrying out sample application, developing and developing, then heating, and inspecting and collecting a thin-layer chromatography image under sunlight and ultraviolet light; carrying out high performance liquid chromatography content determination on the powder sample, preparing a liquid phase test sample solution, carrying out sample injection determination, and judging system adaptability parameters; outputting a qualified or unqualified quality judgment conclusion based on a detection result, and storing related detection data for rechecking and tracing; the problem of quality detection of the Shenqinaokang capsule is systematically solved.