Preparation method and use of hemp seed reference extract product

Abstract

preparation method of a hemp seed reference extract product comprises the following steps: 1, carrying out ether degreasing: taking 200g of hemp seed medicinal material powder, sieving by a No.2 sieve, adding 1500ml of ether, carrying out water bath heating refluxing for 1h at a water bath temperature of 50DEG C, carrying out reduced pressure filtration, adding 500ml to medicinal residues, washing, and removing the obtained ether liquid; 2, extracting with methanol to obtain an extract: adding 1500ml to the obtained medicinal residues, carrying out water bath heating refluxing at 70DEG C for 1h for extraction, carrying out reduced pressure filtration, and evaporating to remove methanol to obtain an extract, and weighing; and 3, carrying out silicon gel mixing and reduced pressure drying: re-dissolving the extract obtained in step 2 in 100ml of methanol, adding column chromatography silica gel having a same amount with the extract, uniformly mixing, carrying out reduced pressure evaporation to remove methanol, and sieving the obtained substance by a No.9 sieve in order to obtain the hemp seed reference extract product. The invention also provides a use of the hemp seed reference extract product. The hemp seed reference extract product substitutes a hemp seed reference medicinal material to be used as a reference for thin layer chromatogram discrimination.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method and application of hemp seed reference extract. Background technique [0002] Traditional Chinese medicine reference substances are indispensable standard reference substances for confirming the quality of traditional Chinese medicine drugs. They are classified into chemical reference substances, reference medicinal materials, and reference extracts according to their chemical properties. Among them, the reference extract was first recorded as a standard substance of traditional Chinese medicine in the 2005 edition of "Chinese Pharmacopoeia". Using it as a reference substance has many advantages for controlling the quality of traditional Chinese medicine: compared with chemical reference substances, it is less difficult to separate and easy to obtain, and the price is relatively low. Inexpensive, to a certain extent, it is equivalent to ...

AI Technical Summary

Problems solved by technology

In the 2010 edition of the Chinese Pharmacopoeia, hemp seed was used as a reference material for the thin layer identification of hemp seed. However, because hemp seed contains a large amount of fatty oil, complex chemical changes are prone to occur during storage, resulting in free fatty acids, excessive Decomposition products such as oxides and low-molecular-weight aldehydes and ketones seriously affect the stability and internal quality of the reference medicinal material; in addition, in the TLC identification, it is still necessary to reflux the hemp seed reference medicinal material through reflux degreasing, filtration, reflux extraction, etc. To obtain the control extract, the process is cumbersome

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