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Method for rapidly and simultaneously detecting ten adulterated components in lipid lowering Chinese patent medicine

A technology for Chinese patent medicines and lipids, which is applied in the detection field of lipid-lowering Chinese patent medicines, can solve the problems of missed detection and non-qualitative detection, and achieve the effects of simple operation, high sensitivity and strong specificity.

Active Publication Date: 2016-01-13
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
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Problems solved by technology

[0004] However, when using this method to detect lipid-lowering Chinese patent medicines, limited by the development conditions of thin-layer chromatography, only a few kinds of adulterated drugs in Chinese patent medicines can be detected at the same time. When there are too many types of adulterated drugs , thin-layer chromatography cannot separate them, and it is impossible to further qualitatively detect them by surface-enhanced Raman spectroscopy, resulting in missed detection, which limits the application of this technology in the field of lipid-lowering Chinese patent medicines to some extent. promotion in

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  • Method for rapidly and simultaneously detecting ten adulterated components in lipid lowering Chinese patent medicine
  • Method for rapidly and simultaneously detecting ten adulterated components in lipid lowering Chinese patent medicine
  • Method for rapidly and simultaneously detecting ten adulterated components in lipid lowering Chinese patent medicine

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preparation example Construction

[0041] (1) Preparation of nano-silver colloid solution: Accurately weigh 8.5mg of PVP (polyvinylpyrrolidone) and 17mg of silver nitrate, dissolve them in 5mL of water, and obtain AgNO with a mass ratio of 2:1. 3 / PVP mixed solution. Measure 50mL of DMF (N,N-dimethylformamide) into a 250mL three-necked bottle, and heat to slightly boiling. Quickly add AgNO as above 3 / PVP solution and continue to boil for a period of time, naturally cool to room temperature and store in a brown bottle away from light and low temperature.

[0042](2) Preparation of a single adulterated component standard solution: Precisely weigh 1 mg of the reference substance and dissolve it in 1 mL of methanol (analytical grade), and use ultrasonic treatment for 30 to 50 minutes to obtain ten adulterated components with a concentration of 1 mg / mL. standard solution.

[0043] (3) Preparation of the matrix solution of lipid-lowering Chinese patent medicine samples: 10 mg of lipid-lowering Chinese patent medi...

Embodiment 1

[0048] In this Example 1, assuming that fenofibrate is contained in lipid-lowering Chinese patent medicines, the method for quickly and simultaneously detecting ten adulterated ingredients in lipid-lowering Chinese patent medicines is explained in detail. The steps are as follows:

[0049] Step 1, preparing matrix solution, reference substance solution (fenofibrate standard solution) and simulated positive sample solution, prepared according to the above method;

[0050] Step 2, determination of surface-enhanced Raman spectrum of fenofibrate standard

[0051] Spot the fenofibrate standard solution of 1mg / mL on the thin-layer plate to obtain spots, and then drop 5uL nano-silver colloid solution on the spots, and use a portable Raman spectrometer to detect the spots on the thin-layer plate. Laser power 300mw, integration time 15s, obtain the surface-enhanced Raman spectrum of this reference substance, specific as figure 2 shown.

[0052] Step 3: Determine the TLC plate develo...

Embodiment 2

[0058] In this second example, assuming that bezafibrate is contained in lipid-lowering Chinese patent medicines, the method for rapid and simultaneous detection of ten adulterated ingredients in lipid-lowering Chinese patent medicines is explained in detail. The steps are as follows:

[0059] Step 1, preparing matrix solution, reference substance solution (bezafibrate standard solution) and simulated positive sample solution, prepared according to the above method;

[0060] Step 2, determination of the surface-enhanced Raman spectrum of the bezafibrate standard

[0061] Spot the fenofibrate standard solution of 1mg / mL on the thin-layer plate to obtain spots, and then drop 5uL nano-silver colloid solution on the spots, and use a portable Raman spectrometer to detect the spots on the thin-layer plate. Laser power 300mw, integration time 15s, obtain the surface-enhanced Raman spectrum of this reference substance, specific as Figure 5 shown.

[0062] Step 3: Determine the TLC ...

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Abstract

The invention provides a method for rapidly and simultaneously detecting ten adulterated components in a lipid lowering Chinese patent medicine. Chromatographic conditions for separation ten adulterated components in the lipid lowering Chinese patent medicine and surface-enhanced Raman spectroscopic detection conditions are rapidly and simultaneously found through a large amount of repeated experiments by adopting a thin layer chromatograph and surface enhanced Raman spectroscopy combination technology, so thin layer chromatograph and surface enhanced Raman spectroscopy combination conditions provided by the invention are suitable for detecting the low concentration adulteration of the lipid lowering Chinese patent medicine and are also suitable for detecting single adulteration or multiple simultaneous adulteration of the ten components, so troubles of every component condition finding needed by simultaneous detection of multiple adulterated components in the prior art are avoided. The method is simple to operate, is suitable for onsite direct detection, and separation and detection conditions provided by the invention have the advantages of simplicity, fastness, high sensitivity and strong specificity.

Description

technical field [0001] The invention belongs to the field of detection of lipid-lowering Chinese patent medicines, in particular to a method for rapidly and simultaneously detecting ten kinds of adulterated ingredients in lipid-lowering Chinese patent medicines. Background technique [0002] Lipid-lowering Chinese patent medicines are popular in the market due to their advantages of less side effects and definite lipid-lowering efficacy. However, driven by profit, some merchants mix lipid-lowering chemical ingredients into lipid-lowering Chinese patent medicines to achieve quick results. Purpose. Patients who take adulterated lipid-lowering Chinese patent medicines for a long time without knowing it will cause a variety of adverse reactions and even endanger their lives. At present, the detection of adulterated Chinese patent medicines is facing three major problems: first, the concentration of adulterated ingredients is extremely low, which requires high sensitivity of det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N30/90
Inventor 陆峰陈梦云方芳李晓朱青霞柴逸峰
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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