The application discloses a detection method of Baizhu San standard decoction
fingerprint combined with liquid
chromatography-
mass spectrometry, the
fingerprint and application thereof, and belongs to the field of
traditional Chinese medicine quality standard research and application.
Test sample solution and single
control sample solution are injected into a high performance liquid chromatograph to obtain respective chromatograms;
mass spectrometry analysis is conducted on the
test sample solution to obtain
mass spectrometry results; common peaks existing in the chromatogram of the
test sample solution are taken as common peaks, chemical components in the Baizhu San standard decoction are identified in combination with the chromatogram of the single
control sample solution and the
mass spectrometry results of the
test sample solution, and the Baizhu San standard decoction
fingerprint is obtained. The fingerprint is constructed through liquid
chromatography-
mass spectrometry, 10 intersecting components are identified in combination with the
mass spectrometry information of the
control sample, so that the
quality control of the Baizhu San standard decoction is realized, the method is simple, easy to operate, high in precision, good in stability and reproducibility, and can effectively solve the technical problem that the quality of the Baizhu San cannot be effectively evaluated.