Method for measuring content of components of prince's-feather herb

A determination method and multi-component technology, which can be used in material separation, pharmaceutical formulations, medical preparations containing active ingredients, etc., and can solve the problem of less research on oriental herbs.

Inactive Publication Date: 2010-09-08
GUIYANG MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, through literature research, it is found that there are few studies on Chinese herbal medicines, and the quality standards of Chinese herbal medicines in Guizhou Province do not include methods such as inspection and content determination.

Method used

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  • Method for measuring content of components of prince's-feather herb
  • Method for measuring content of components of prince's-feather herb
  • Method for measuring content of components of prince's-feather herb

Examples

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Embodiment 1

[0113] Example 1: Catechin, isoorientin, orientin, quercetin-3-O-(2″-O-α-rhamnose)-β-glucoaldehyde, Determination of Eight Compounds Taxifolin, Luteolin, Quercitrin and Kaempferol-3-O-β-3-D-glucoside by HPLC

[0114] 1. Chromatographic conditions and system suitability test

[0115] Instrument: ACQUITY UPLC (Waters, USA, including binary gradient pump, vacuum degasser, autosampler, column oven, diode array detector, Empower 2 workstation);

[0116] Chromatographic column: ACQUITY UPLC BEH C18 column (2.1×150mm, 1.7μm);

[0117] Mobile phase: A is acetonitrile, B is 0.1% H 3 PO 4 , carry out gradient elution according to the regulations in Table 1;

[0118] Flow rate: 0.3mL / min;

[0119] Column temperature: 45°C;

[0120] Detector: array diode detector;

[0121] Detection wavelength: 200nm, 350nm;

[0122] Injection volume: 1μL

[0123] Table 1 Mobile phase gradient elution table

[0124]

[0125] 2. Preparation of reference substance solution Weigh respectively 10...

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Abstract

The invention provides a method for measuring content of components of prince's-feather herb and belongs to the technical field of medicament content measurement. High performance liquid chromatography is adopted to measure the content of eight compounds, namely catechin, homo-orientin, orientin, quercetin-3-O-(2'-O-alpha-rhamnose)-betal-glucal, texifolin, luteolin-7-O-beta-D-glucoside, quercetin and kaempferide-3-0-beta-3-D-glucoside, in the prince's-feather herb under the same chromatographic condition. Compared with the conventional single component content measuring method, the method of the invention is more integral, characteristic and stable, and provides test basis for further improving the quality of the prince's-feather herb and the re-development and application of the prince's-feather herb. The invention also provides the methods for preparing reference solution and test sample solution.

Description

technical field [0001] The invention relates to a multi-component content determination method of Chinese medicinal materials, belonging to the technical field of content determination of traditional Chinese medicinal materials. Background technique [0002] Orion is the dried fruit ear and leafy stem of Polygonum orientale L., a plant of the Polygonaceae family. Harvested in autumn and sun-dried. It is a commonly used herbal medicine in Guizhou. It was first seen in ancient books such as "Bie Lu", "Ben Cao Tu Jing", "Compendium of Materia Medica" and other ancient books. ", "Chinese Herbal Medicine", "Southern Yunnan Materia Medica" and other documents are recorded. In 1988, it was included in the "Quality Standards of Chinese Medicinal Materials of Guizhou Province" (1988 edition) with "Dried Whole Herb of Orchard (Red Grass)". In 1992, it was included in "Guizhou Traditional Chinese Medicine Resources" with "Polygonum orientalis (Polygonum orientalis, Polygonum chinens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/704G01N30/02A61P1/00A61P9/10A61P17/00A61P19/02A61P29/00
Inventor 王爱民王永林李勇军兰燕宇何迅廖尚高迟明艳周雯
Owner GUIYANG MEDICAL UNIVERSITY
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