94results about How to "Increase sampling volume" patented technology

A device and method for lancing a patient, virtually simultaneously producing and collecting a small fluid sample from a body. The device comprises a blood collection system including a lancing needle (16), drive mechanism (11), kneading or vibration mechanism (25), optional suction system (7), and sample ejection mechanism. The device is preferably sized to be hand-held in one hand and operable with one hand. The device can optionally contain integral testing or analysis component (83) for receiving the sample and providing testing or analysis indication or readout for the user. A method involves piercing the skin at a rapid rate, kneading the surrounding area by ultrasonic action, piezoelectric or mechanical oscillation to stimulate the blood flow from the wound, drawing the fluid using a pumping system.

System and method for capturing, concentrating, and detecting a diagnostic target in a liquid, comprising applying a magnetic field to a mixture comprising a co-aggregate in the liquid to provide a collected co-aggregate in the liquid, wherein the co-aggregate comprises a magnetic particle having a stimuli-responsive polymer attached thereto and a non-magnetic particle having a stimuli-responsive polymer and a diagnostic target attached thereto.

The invention discloses a soil layering scraping sampler. The sampler comprises a rectangular sampling frame and a rectangular scraping plate capable of scraping soil slidably along the sampling frame, wherein depth limiting devices are arranged on both sides of the scraping plate. The soil layering scraping sampler can control the thickness of soil layering accurately and obtain a large number of soil samples at a time, and has the advantages of simple operation and convenient carrying.

The present invention relates to a two-dimensional preparative chromatography instrument system having the online enrichment function, and applications thereof. The system comprises a chromatography flow channel switching valve, a chromatography column, a solvent pump and a detector. According to the present invention, a trapping column collects a first-dimensional chromatography effluent component through valve switching and mobile phase dilution, and the trapped component is introduced into a second-dimensional preparative column through valve switching and mobile phase elution so as to achieve two-dimensional chromatography separation; the instrument system can perform two-dimensional separation, and the sample and the one-dimensional separation component are effectively retained and enriched through the trapping column interface, such that the column efficiency loss is low, and the two-dimensional separation effect is ensured; and the instrument system can enlargedly perform multiple circulation separations and multidimensional separation, and the separation effect can not be decreased along with the increasing sample volume and the increasing separation frequency. In addition, the instrument system further has the function of the concentration recovery on the finally-separated product so as to simplify the sample drying process, such that the degeneration of the sample during the heating drying process is easily be avoided.

The invention mainly relates to a method for extracting purified dendrobine from dendrobium stem. The method comprises the following steps of: firstly, extracting with acid alcohol, enriching and purifying extract by cation exchange resin, stripping with an organic solvent, and separating and preparing by HPLC (High Performance Liquid Chromatography). The stable extract with the dendrobine content not less than 90% is prepared by the method, the extract is furthered prepared by the HPLC, and the dendrobine with the purity not less than 95% is obtained. Compared with the traditional process, the invention has the advantages of high selectivity, moderate reaction condition, simple process, short production period, and high dendrobine yield and purity. By utilizing the HPLC to separate and prepare, the dendrobine loss can be reduced, the relevant matters with similar structure can be obtained, the practical value is high, and a new path by utilizing the dendrobium stem plants is provided.

A nuclear magnetic resonance (NMR) sample tube is made of a polymeric material instead of glass. Such tubes are thinner than glass tubes, thus increasing the internal volume and sample size. Such tubes are also more closely matched to the magnetic susceptibility of specific solvents. Such tubes have greater mechanical stability, thus leading to less tube breakage during NMR processing. Such tubes also lend themselves to various concentric tubal arrangements which permit separation and mixing of samples to minimize subtraction artifacts in interacting systems.

A headspace sampling apparatus (10) operates to provide a sample of a vapor phase of a substance being analyzed under conditions of thermodynamic equilibrium. The substance is held in a vial (14). The vapor phase of the substance (18) is moved through a sample loop (34) between a first variable volume chamber (22) and a second variable volume chamber (40). A constant volume and temperature is maintained within the variable volume chambers, the sample loop and the headspace of the vial. An aliquot of the equilibrated vapor in the sample loop is passed to an analytical instrument (54) for analysis. The sample is obtained under conditions in which the sampling process does not affect the makeup of the sample because thermodynamic equilibrium is maintained.

The invention discloses probes, primers, a detection system and a kit for detecting mutations of an EGFR gene, and the probes and the primers have the sequences of SEQ ID NO.1 to SEQ ID NO.24. The invention is characterized in that (1), the amplification efficiency is greatly improved to a largest extent; (2) the sensitivity is high, and the detection sensitivity can reach 0.2%; (3) compared with a digital PCR method, operations are simple, the cost is saved, and the clinical application scope is wide; (4) plasma samples with large reaction volume are detected, the DNA sampling quantity of the plasma samples becomes larger, the system is more stable, and the detection rate of the plasma samples is increased; (5) 25 mutations of the EGFR gene can be detected simultaneously in three reaction tubes, and results are intuitive and clear; (6) the detection speed is fast, and consumed time is only 1/2 that of the digital PCR; and (7) a detection method can detect peripheral blood samples, has convenient sampling, and can dynamically monitor curative effect of EGFR-TKI drugs on patients.

The method includes: providing a test strip comprising a reference electrode and a working electrode coated with a reagent layer; applying a fluid sample to the test strip for a reaction period; applying a test voltage between the reference electrode and the working electrode; measuring a test current as a function of time; measuring a steady state current value when the test current has reached an equilibrium; calculating a ratio of the test current to the steady state current value; plotting the ratio of the test current to the steady state current value as a function of the inverse square root of time; calculating an effective diffusion coefficient from the slope of the linearly regressed plot of the ratio of the test current to the steady state current value as a function of the inverse square root of time; and calculating a hematocrit-corrected concentration of analyte.

A sensitivity-enhanced flow cell to be used in the determination of the differential refractive index increment of a sample fluid relative to a reference fluid is disclosed. The invention permits the use of smaller sample amounts without sacrificing overall sensitivity. Equally important, said improved flow cell produces measurements of increased precision without requirement for increased sample amount. This is achieved by means of two chambers within said cell whose volumes are different. The sample fluid chamber is the smaller of the two with the reference fluid chamber constructed so that the incident illumination beam, upon passage through said sample chamber and displacement by the partition element located therebetween said sample and reference chambers, passes through said reference chamber without grazing any of the confining walls or striking corners of said sensitivity-enhanced flow cell. As the amount of deflection of said transmitted beam depends upon the refractive indices of the fluids relative to the transparent matter of which the containing cell is comprised, as well as the RI difference between said fluids themselves, the reference chamber is designed to transmit said incident light beam without grazing degradation thereof for all practical ranges of expected fluid and cell refractive indices.

The invention discloses a three-dimensional biomarker detection device, a preparation method and a method for detecting a biomarker. The three-dimensional biomarker detection device includes a three-dimensional porous scaffold chip and a sample carrier plate; wherein the three-dimensional porous scaffold chip comprises a substrate and a plurality of three-dimensional porous scaffolds, the plurality of three-dimensional porous scaffolds are formed on the upper surface of the substrate; the sample carrier plate comprises a base, a plurality of hydrophilic regions and a plurality of hydrophobic regions, the plurality of hydrophilic regions are formed on the upper surface of the base, the plurality of hydrophobic regions are formed on the upper surface of the base, the plurality of hydrophilic regions and the plurality of hydrophobic regions are arranged at intervals, and the plurality of hydrophilic regions are corresponding to the plurality of the three-dimensional porous scaffolds. The three-dimensional biomarker detection device can effectively detect biomarkers.

The invention provides a puncture forceps holder biopsy needle used beneath a bronchoscope. The puncture forceps holder biopsy needle is composed of an outer sleeve tube, a puncture needle, biopsy forceps, a tensioning steel wire, a connecting steel wire, and a sliding block, a helical casing and a helical connector which correspondingly control the puncture needle and the biopsy forceps, a sliding block and a handle which control the tensioning steel wire and the like. In the outer sleeve tube of the puncture forceps holder biopsy needle, the puncture needle is connected with a puncture needle sliding block through the connecting steel wire, the biopsy forceps are located in the puncture needle and connected with the biopsy forceps sliding block through the tensioning steel wire. According to the puncture forceps holder biopsy needle, transbronchial needle aspiration and forcep biopsy can be achieved on the same puncture part, the quantity of obtained samples can be increased, the diagnostic rate of mediastinal lesions is increased, meanwhile the discomfort of a patient is greatly reduced, and the safety of the patient is improved.

The embodiment of the invention discloses an ore sample preparation system, which comprises a rack, a crusher, a chute and a reduction and division device, wherein the crusher and the reduction and division device are arranged on the rack; the crusher is positioned above the reduction and division device; a discharge port of the crusher is communicated with a feed port of the reduction and division device through the chute; the crusher is externally connected with transfer equipment to realize the input of materials, and the materials directly enters the reduction and division device through the chute after being crushed into required granularity by the crusher; the reduction and division device has a material uniform-mixing function, and the materials can be uniformly stirred and mixed before being reduced and divided, so that the accuracy of reduction and division ratio of the reduction and division device is ensured. Compared with the technical scheme in the prior art, the ore sample preparation system has the advantages that manual sample preparation, manual loading and manual sampling are not required, so that the sample preparation capacity can be improved; the crushed materials in the crusher are directly guided into the reduction and division device through the chute without external conveying equipment for conveyance, so that the space occupied by the system is reduced.

The invention proposes a sensor device (10) and a method of sampling. The method comprises the steps of: A. adding a sample to a chamber (12) in which magnetic particles (P) are provided, the sample including a target component (T) and the chamber (12) having a detection surface (122); B. exerting a magnetic force on the magnetic particles (P) to attract the magnetic particles (P) to the detection surface (122), wherein bound magnetic particles that capture the target component (T) in the magnetic particles (P) are held at the detection surface (122), and unbound magnetic particles that capture no target component (T) in the magnetic particles (P) are held at the detection surface (122) as well; C. altering the magnetic force exerted on the magnetic particles (P) to release the unbound magnetic particles from the detection surface (122); D. measuring the amount of the bound magnetic particles that are held at the detection surface (122); E. determining whether the amount of the bound magnetic particles that are held at the detection surface (122) is above a predetermined threshold; if no, performing the following steps of: exerting the magnetic force on the magnetic particles to attract the magnetic particles (P) to the detection surface (122); draining at least part of the sample out of the chamber (12) and adding a new sample to the chamber (12); altering the magnetic force exerted on the magnetic particles to release the unbound magnetic particles from the detection surface (122); and going back to perform the steps B to E. By doing so, the target component (T) is preconcentrated by repeating the steps of magnetically binding the target component (T) from the newly added sample and washing the detection surface (122) from unbound magnetic particles.

The invention discloses a device for detecting the carbon content of fly ash in a coal-fired power generation boiler and belongs to the technical field of detection of carbon content of fly ash. The device for detecting the carbon content of the fly ash in the coal-fired power generation boiler comprises a drying furnace, a burning furnace, a burning container and a lifting rotary tray, wherein one end of the drying furnace is communicated with an inert gas supply mechanism through a pipeline, and the other end of the drying furnace is communicated with a vacuum pump I through a pipeline; oneend of the burning furnace is communicated with an oxygen supply mechanism through a pipeline, the other end of the burning furnace is communicated with one end of a sealed container through a pipeline, and the other end of the sealed container is communicated with a vacuum pump II through a pipeline; the lifting rotary tray is used for transporting the burning container. The invention aims to overcome the defects of low measurement accuracy and reliability of the conventional technology for detecting the carbon content of the fly ash in the coal-fired power generation boiler, provides the device for detecting the carbon content of the fly ash in the coal-fired power generation boiler, and improves the accuracy and the reliability of measurement on the carbon content of the fly ash.

Provided herein are integrated devices that improve sensitivity of rapid diagnostic tests. The devices described herein integrate sample separation with rapid diagnostic tests and improve signal intensity of the rapid diagnostic tests.

The invention relates to a device (1), a method, and a kit for analysing liquid samples. The device (1) comprises a sample layer (111) having a plurality of liquid permeable test sites (112) separated by a liquid impermeable barrier region (113), and an inlet part (2) comprising a plurality of inlet channels (211), which lead to respective test sites (112) of the sample layer (111), such that a flow connection between said inlet channels (211) and said respective test sites (112) is established or can be established, wherein said inlet channels (211) comprise first openings (218) and second openings (219), wherein a second surface area defined by the positions of said second openings (219) is smaller than a first surface area defined by the positions of said first openings (218) The invention further relates to a method for functionalizing a sample layer (111).