94results about How to "Increase sampling volume" patented technology

The invention discloses a soil layering scraping sampler. The sampler comprises a rectangular sampling frame and a rectangular scraping plate capable of scraping soil slidably along the sampling frame, wherein depth limiting devices are arranged on both sides of the scraping plate. The soil layering scraping sampler can control the thickness of soil layering accurately and obtain a large number of soil samples at a time, and has the advantages of simple operation and convenient carrying.

An optical assay device for the detection of an analyte of interest in a sample comprising a support containing channels, an optically functional layer positioned on the support such that the optically functional layer and the support allow for laminar flow of the sample through layers of the device, an attachment layer positioned on the optically functional layer, and an analyte specific receptive layer positioned on the attachment layer.

A sensitivity-enhanced flow cell to be used in the determination of the differential refractive index increment of a sample fluid relative to a reference fluid is disclosed. The invention permits the use of smaller sample amounts without sacrificing overall sensitivity. Equally important, said improved flow cell produces measurements of increased precision without requirement for increased sample amount. This is achieved by means of two chambers within said cell whose volumes are different. The sample fluid chamber is the smaller of the two with the reference fluid chamber constructed so that the incident illumination beam, upon passage through said sample chamber and displacement by the partition element located therebetween said sample and reference chambers, passes through said reference chamber without grazing any of the confining walls or striking corners of said sensitivity-enhanced flow cell. As the amount of deflection of said transmitted beam depends upon the refractive indices of the fluids relative to the transparent matter of which the containing cell is comprised, as well as the RI difference between said fluids themselves, the reference chamber is designed to transmit said incident light beam without grazing degradation thereof for all practical ranges of expected fluid and cell refractive indices.

A method for measuring an sample in an NMR spectrometer uses a coupling configuration (1; 30) comprising a coupling element (3) and a supply capillary (2), wherein the coupling element (3) has a funnel-shaped section (5) which clamps an end (6) of the supply capillary (2). An envelope capillary (10) is provided into which the supply capillary (2) is inserted, wherein the end (6) of the supply capillary (2) projects past an end (11) of the envelope capillary (10), and the end (11) of the envelope capillary (10) is also clamped in the funnel-shaped section (5).

The invention proposes a sensor device (10) and a method of sampling. The method comprises the steps of: A. adding a sample to a chamber (12) in which magnetic particles (P) are provided, the sample including a target component (T) and the chamber (12) having a detection surface (122); B. exerting a magnetic force on the magnetic particles (P) to attract the magnetic particles (P) to the detection surface (122), wherein bound magnetic particles that capture the target component (T) in the magnetic particles (P) are held at the detection surface (122), and unbound magnetic particles that capture no target component (T) in the magnetic particles (P) are held at the detection surface (122) as well; C. altering the magnetic force exerted on the magnetic particles (P) to release the unbound magnetic particles from the detection surface (122); D. measuring the amount of the bound magnetic particles that are held at the detection surface (122); E. determining whether the amount of the bound magnetic particles that are held at the detection surface (122) is above a predetermined threshold; if no, performing the following steps of: exerting the magnetic force on the magnetic particles to attract the magnetic particles (P) to the detection surface (122); draining at least part of the sample out of the chamber (12) and adding a new sample to the chamber (12); altering the magnetic force exerted on the magnetic particles to release the unbound magnetic particles from the detection surface (122); and going back to perform the steps B to E. By doing so, the target component (T) is preconcentrated by repeating the steps of magnetically binding the target component (T) from the newly added sample and washing the detection surface (122) from unbound magnetic particles.

The invention discloses probes, primers, a detection system and a kit for detecting mutations of an EGFR gene, and the probes and the primers have the sequences of SEQ ID NO.1 to SEQ ID NO.24. The invention is characterized in that (1), the amplification efficiency is greatly improved to a largest extent; (2) the sensitivity is high, and the detection sensitivity can reach 0.2%; (3) compared with a digital PCR method, operations are simple, the cost is saved, and the clinical application scope is wide; (4) plasma samples with large reaction volume are detected, the DNA sampling quantity of the plasma samples becomes larger, the system is more stable, and the detection rate of the plasma samples is increased; (5) 25 mutations of the EGFR gene can be detected simultaneously in three reaction tubes, and results are intuitive and clear; (6) the detection speed is fast, and consumed time is only 1 / 2 that of the digital PCR; and (7) a detection method can detect peripheral blood samples, has convenient sampling, and can dynamically monitor curative effect of EGFR-TKI drugs on patients.

The invention relates to a device (1), a method, and a kit for analysing liquid samples. The device (1) comprises a sample layer (111) having a plurality of liquid permeable test sites (112) separated by a liquid impermeable barrier region (113), and an inlet part (2) comprising a plurality of inlet channels (211), which lead to respective test sites (112) of the sample layer (111), such that a flow connection between said inlet channels (211) and said respective test sites (112) is established or can be established, wherein said inlet channels (211) comprise first openings (218) and second openings (219), wherein a second surface area defined by the positions of said second openings (219) is smaller than a first surface area defined by the positions of said first openings (218) The invention further relates to a method for functionalizing a sample layer (111).