EGCG preparation method

A technology of extraction solution and extraction tank, which is applied in the field of separation and purification of plant monomers, can solve problems such as insufficient safety and environmental protection, complicated process, etc., and achieve the effects of reduced use and recycling, good membrane stability, and enhanced adsorption and exchange

Active Publication Date: 2017-07-21
盛林
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Problems solved by technology

[0006] The present invention aims at the problems that the preparation process of EGCG by the organic solvent extraction method is complex, a large amount of organic solvents are used in the production process, and it is not safe and environmentally friendly, and provides a preparation method suitable for industrial production of high-purity EGCG, which reduces a large amount of organic solvents in the production process The use of environmental protection, and improve the purity of the product

Method used

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Embodiment 2

[0039] The preparation of embodiment 2 technical grade high-purity EGCG

[0040] (1) Grind the green tea with a pulverizer, weigh 100kg green tea powder, put it into a 1200L extraction tank, add 1000L deionized water, heat up to 90°C, stir at 50r / min, and vacuum extract at 0.12Mpa for 20min.

[0041](2) Take out 250L of the extraction liquid in the extraction tank with a suction filter tube, add 250L of ionized water into the tank and repeat the extraction twice according to step (1).

[0042] (3) Combine the extracts from steps (1) and (2), cool to 50°C, and use Al with a pore size of 0.8 μm 2 o 3 Filtrate with a ceramic membrane filter at 0.15Mpa to obtain filtrate and filter residue, discard the filter residue.

[0043] (4) Take the filtrate of step (3), import it into a vacuum concentrator, concentrate in a vacuum at 40° C. and 0.09 MPa for 90 minutes, and obtain 120 L of concentrated extract; take the extract sample and dry and weigh it, and the calculated extraction ra...

Embodiment 3

[0048] Embodiment 3 adopts HPLC method to detect EGCG purity

[0049] (1) Detection conditions

[0050] Instrument model: liquid chromatograph Agilent 1260

[0051] Chromatographic column: Agilent Poroshell 120SB-C18 (4.6×100mm, 2.7μm)

[0052] Mobile phase: acetonitrile-0.1% formic acid in water

[0053] Flow rate: 1.0mL / min

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Abstract

The invention provides an EGCG preparation method, particularly relates to a preparation method for industrial production of high-purity EGCG and belongs to the field of separation and purification of plant monomers. For solving the problem that at present, a process for preparing the EGCG based on an organic solvent extraction method is complicated, adopts a large number of organic solvents in the production process and is not safe and environment-friendly enough, the EGCG preparation method comprises the following steps of green tea smashing, vacuum high-temperature extraction with deionized water, inorganic ceramic membrane filtration, enrichment with macroporous adsorption resin, reversed phase chromatography, vacuum concentration and freeze-drying. The method only adopts water, aqueous ethanol and other solvents in the whole preparation process of the process, the usage and recovery amount of a large amount of organic solvents is decreased, the non-toxic, safe and simple process is achieved, ceramic membrane, resin and reverse chromatographic column filler are recyclable, the cost is greatly reduced, meanwhile the purity of the EGCG is improved, and the process is more suitable for large-scale EGCG production.

Description

technical field [0001] The invention relates to a preparation method of EGCG, in particular to a preparation method suitable for industrial production of high-purity EGCG, and belongs to the field of separation and purification of plant monomers. Background technique [0002] EGCG (epigallocatechin gallate), the Chinese name of epigallocatechin gallate, is the main component and active ingredient of green tea tea polyphenols (TeaPolyphenols, TP), and has anti-oxidation, anti-cancer, anti-mutation and other activities. EGCG is a white amorphous crystalline substance, easy to oxidize and polymerize, generally light yellow to brown; slightly tea-like, with astringent taste; easily soluble in water, ethanol, methanol, ethyl acetate, acetone, etc., slightly soluble in oil , insoluble in organic solvents such as benzene and chloroform; can form green-black compounds with iron ions. [0003] In fresh tea leaves, the water content is about 75%, and the dry matter is 25%. In the dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 盛林迟宝琦
Owner 盛林
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