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Extracting and purifying method for hypericum perforatum component in plant

A technology of hypericin and purification method, which is applied in the fields of quinone separation/purification, organic chemistry, etc., and can solve the problem that the flowers of Hypericum perforatum are difficult to obtain in large quantities, and achieve the effects of increasing sample loading, reducing pollution, and avoiding interference

Inactive Publication Date: 2003-01-22
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the flower of Hypericum perforatum is used for extraction, and the hypericin content in the obtained extract reaches 4-7%, but it is difficult to obtain a large amount of Hypericum perforatum flower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Get Hypericum perforatum medical material powder 300g, each with 800 milliliters containing 1% composite stabilizer (the composition of stabilizer is V C :V E : citric acid=50: 100: 1) acetone leaching, extract 3 times altogether, each time 1 day, combine acetone extract, reclaim acetone to add 250 milliliters of distilled water after 1000 milliliters. Extract with 1300 milliliters of petroleum ether, extract 2 times in total, combine petroleum ether phase and add vitamin E to the content of 1%. Concentrate to 600 ml and extract twice with 500 ml of 50% ethanol, and discard the ethanol phase. The petroleum ether phase was basified with 800 ml of 1% NaOH in 50% ethanol, and the petroleum ether phase was discarded. Use 10% hydrochloric acid to adjust the alkali alcohol solution to pH=2, then use V E Extract 3 times with 1% petroleum ether, each with 300 ml of petroleum ether. The petroleum ether phases were combined, concentrated at low temperature and reduced pressur...

Embodiment 2

[0031] Get Hypericum perforatum medical material powder 534g, each with 1200 milliliters of acetone (containing 1% composite stabilizer, the composition of stabilizer is V C :V E : citric acid=100:50:1) leaching under constant stirring, extracting 3 times altogether, each time 2 hours, combining acetone extracts, reclaiming acetone to 930 milliliters, adding distilled water 260 milliliters. Then use 900ml containing V E 2% n-hexane extraction, a total of 2 extractions, combined n-hexane phase. Concentrate to 860 ml and extract twice with 400 ml of 50% ethanol, and discard the ethanol phase. The petroleum ether phase was basified with 1000 ml of 1% NaOH in 50% ethanol solution, and the petroleum ether phase was discarded. Use 10% hydrochloric acid to adjust the alkali-alcoholic solution to pH=2, then use 500 ml of V E2% n-hexane extraction, a total of 2 extractions, combined n-hexane phase. Concentrate at low temperature and reduced pressure to obtain 3.47 g of a light bro...

Embodiment 3

[0033] Get 1100g Hypericum perforatum medical material and pulverize, under stirring each time with 2200ml acetone (containing 1% compound stabilizer, the composition of stabilizer is V C Palmitate: V E : Benzoic acid=200:50:1) leaching, extract 3 times altogether, each time 2 hours, combine extracting solution. After the extract was concentrated to 3000 ml, 800 ml of distilled water was added, and extracted with petroleum ether containing VE 0.5%. The petroleum ether phase was concentrated to 2100 ml, extracted once with 700 ml of 60% ethanol, and the alcoholic aqueous phase was discarded. 2000 ml 2% NaHCO 3 50% ethanol solution extraction, the alkali-alcohol phase was adjusted to pH = 0 with 10% hydrochloric acid, and then extracted with 1000 ml of petroleum ether containing VE 0.5%, a total of 2 extractions, the petroleum ether phase was combined and concentrated under reduced pressure at room temperature to obtain Brown extract 6.3g, hypericin content 68.2%.

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Abstract

The present invention relates to the process of extracting and purifying Hyperforin from Hypericum perforatum or other congenetic plant. The process includes poalr solvent extraction or biphase extraction or non-polar organic solvent extraction, impurity elimination with alcoholic water and acid and base treatment and the extract has Hyperforin content not less than 65%. Through further silica column chromatography and HPLC preparation, Hyperforin product with purity higher than 95% or 98% may be produced.

Description

technical field [0001] The invention relates to a method for extracting and purifying hyperforin components from Hypericum perforatum or other plants containing hyperforin. Background technique [0002] Hypericum perforatin (Hyperforin) mainly exists in Hypericum perforatum L. and other plants of the same genus. The compound has antidepressant and antibacterial effects. Muller et al. proved that hypericin in nanomolar concentration (10 -9 mol / l) level can not only inhibit the reuptake of 5-HT, NE, DA, but also induce the negative regulation of cortical β-adrenoceptor expression. Muller's research shows that hyperforin, like traditional 5-HT and NE inhibitors, can inhibit the reuptake of all three monoamines. Studies by Bhattacharya et al. have shown that hyperforin has similar activity to antidepressants and anxiolytics in experimental models of animal depression, and hyperforin can improve the antidepressant ability of animals in force...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C46/10C07C50/36
Inventor 杨得坡王发松黄世亮王冬梅黄亚东黄巧娟
Owner SUN YAT SEN UNIV
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