Method for extracting purified dendrobine from dendrobium stem

A technology of Dendrobium nobile and Dendrobine, which is applied in the field of preparation of Chinese medicine extracts, can solve the problems that the quality of Dendrobium is not stable enough, is not suitable for industrial production, and contains many impurities, so as to increase the amount of sample loading and simplify the separation and purification steps. , the effect of reducing pollution

Active Publication Date: 2010-06-16
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, the extraction of dendrobine is mainly according to the literature (T.Okamoto, et al.The structure ofdendrobine.Chem.Pharm.Bull.1964,12(4):506-512; Y.Inubushi, et al. the Chinese Drug "Chi-shi-hu".Chem.Pharm.Bull.1964,12(10):1175-1180), but the quality of dendrobine obtained by this method is not stable enough, and contains more impurities. Low recovery rate, not suitable for industrial production

Method used

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  • Method for extracting purified dendrobine from dendrobium stem
  • Method for extracting purified dendrobine from dendrobium stem
  • Method for extracting purified dendrobine from dendrobium stem

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Embodiment 1

[0039] Take the stems of Dendrobium nobile, extract 10 times the dry weight of medicinal materials with acidic ethanol at pH=3 for 2 hours in a slightly boiling state, extract 3 times, concentrate the extract into an extract, add ammonia water to adjust pH=10, and use an equal volume of Extract 3-4 times with chloroform, concentrate the extract, and freeze-dry to obtain the total alkaloids of Dendrobium nobile with a content of 68.4%. Reconstitute the obtained total alkaloids of Dendrobium nobile with acidic water of pH=4, add ammonia water to adjust pH=10, extract with an equal volume of ether until the ether is colorless, concentrate and freeze-dry to obtain dendrobine, and the content of dendrobine is 66.1%.

Embodiment 2

[0041] Get the stem of Dendrobium nobile, extract 10 times of the dry weight of the medicinal material with acidic ethanol with pH=3 for 2 hours in a slightly boiling state, and extract 3 times, the extract is concentrated into an extract, dissolved in acidic water with pH=4, over The pretreated cation exchange resin is firstly eluted with distilled water for water-soluble impurities, and then eluted with 70% alkaline ethanol. The eluate is concentrated until there is no alcohol smell, and ammonia water is added to adjust the pH to 10, and extracted with an equal volume of chloroform 3 4 The second time, the extract was concentrated and freeze-dried to obtain the total alkaloids of Dendrobium nobile with a content of 81.0%. Reconstitute the obtained total alkaloids of Dendrobium nobile with acidic water of pH=4, add ammonia water to adjust pH=10, extract with an equal volume of ether until the ether is colorless, concentrate and freeze-dry to obtain dendrobine, and the content ...

Embodiment 3

[0043] Get the stem of Dendrobium nobile, extract 10 times of the dry weight of the medicinal material with acidic ethanol with pH=3 for 2 hours in a slightly boiling state, and extract 3 times, the extract is concentrated into an extract, dissolved in acidic water with pH=4, over After pretreatment of the cation exchange resin, the water-soluble impurities were first eluted with distilled water, and then 70% alkaline ethanol was used to elute the eluent. 4 times, concentrate the extract, and freeze-dry to obtain the total alkaloids of Dendrobium nobile with a content of 85.3%. The total alkaloids obtained from Dendrobium nobile were reconstituted with acidic water at pH=4, adjusted to pH=10 by adding ammonia water, extracted with an equal volume of ether until the ether was colorless, concentrated and freeze-dried to obtain the crude extract of dendrobine. Dissolve with 3 times the amount of methanol, then perform HPLC separation and purification, collect the components with ...

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Abstract

The invention mainly relates to a method for extracting purified dendrobine from dendrobium stem. The method comprises the following steps of: firstly, extracting with acid alcohol, enriching and purifying extract by cation exchange resin, stripping with an organic solvent, and separating and preparing by HPLC (High Performance Liquid Chromatography). The stable extract with the dendrobine content not less than 90% is prepared by the method, the extract is furthered prepared by the HPLC, and the dendrobine with the purity not less than 95% is obtained. Compared with the traditional process, the invention has the advantages of high selectivity, moderate reaction condition, simple process, short production period, and high dendrobine yield and purity. By utilizing the HPLC to separate and prepare, the dendrobine loss can be reduced, the relevant matters with similar structure can be obtained, the practical value is high, and a new path by utilizing the dendrobium stem plants is provided.

Description

technical field [0001] The invention belongs to the preparation of traditional Chinese medicine extracts, and mainly relates to a method for extracting and purifying dendrobine from dendrobium nobile. technical background [0002] Dendrobium has the effects of anti-aging, anti-tumor, lowering blood sugar, treating gastrointestinal diseases and treating cataracts. The medicinal ingredients of Dendrobium are mainly dendrobine and polysaccharides. The content of total alkaloids in 11 species of Dendrobium was determined and found The content of alkaloids with a bitter taste is relatively high when the traits are identified, and the total alkaloid content of Dendrobium nobile is 0.41% to 0.64%, which is much higher than that of other species of Dendrobium, mainly including dendrobine, dendrobine, 6-hydroxy dendrobium, etc. . Its main component, dendrobine, has analgesic and antipyretic effects, which can lower heart rate and blood pressure, slow down breathing, moderately incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/18
Inventor 刘婵石京山吴芹
Owner ZUNYI MEDICAL UNIVERSITY
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