Preparation method of standard radix pseudostellariae cyclopeptide B

A technology of Pseudostellariae and standard products, which is applied in the field of medicine, can solve the problems such as the preparation method of Pseudostellaria cyclic peptide B that has not been seen, and achieve the effects of rapid identification, high throughput and rapidity, reduction of pollution, and simplification of material separation and purification steps

Inactive Publication Date: 2014-07-16
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are few domestic and foreign reports on the preparation process of the standard Pseudostellaria cyclic peptide B, and there is no preparation method for Pseudostellaria cyclic peptide B that can be applied to industrial production in the prior art

Method used

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  • Preparation method of standard radix pseudostellariae cyclopeptide B
  • Preparation method of standard radix pseudostellariae cyclopeptide B
  • Preparation method of standard radix pseudostellariae cyclopeptide B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Take 500g of Radix Pseudostellariae Radix Pseudostellariae as raw material, crush it to a particle size of 60 mesh, add 6 times the volume of 95% industrial ethanol, and conduct heat reflux extraction at 80°C for 3 hours. After extraction, the extract is filtered out. Repeat the above process 2 more times, combine the extracts three times, concentrate under reduced pressure to a specific gravity of 1.15, and obtain 65.00 g of ethanol extract, and the content of Pseudostellaria cyclic peptide B is 0.23%. Add 95% industrial ethanol heat reflux dissolving 2h of 10 times of volumes to medicinal extract, cool and stand for 10h, filter, collect filtrate, concentrate and recover solvent and obtain 16.50g of total cyclic peptide extract of Radix Pseudostellariae, the content of Pseudostellariae cyclic peptide B is 0.86%, the enrichment rate is 93.09%.

[0031] (2) Enrichment of Pseudostellaria cyclic peptide B by silica gel column chromatography: the above total cyclic pept...

Embodiment 2

[0034] (1) Take 500g of Pseudostellaria Pseudostellariae medicinal material, crush it to a particle size of 80 mesh, add 5 times the volume of 95% industrial ethanol, and conduct heat reflux extraction at 85°C for 3 hours. After extraction, the extract is filtered out, and then The above process was repeated twice, the extracts were combined three times, concentrated under reduced pressure to a specific gravity of 1.2, and 64.50 g of ethanol extract was obtained, and the content of Pseudostellaria cyclic peptide B was 0.24%. Add 9 times the volume of 95% industrial ethanol to the extract and dissolve it under reflux for 2 hours, cool and stand for 10 hours, filter, collect the filtrate, concentrate and recover the solvent to obtain 17.50 g of the total cyclic peptide extract of Radix Pseudostellariae, and the content of Pseudostellaria cyclic peptide B is 0.84 %, the enrichment rate was 93.44%.

[0035] (2) Enrichment of Pseudostellaria cyclic peptide B by silica gel column chro...

Embodiment 3

[0038] (1) Take 500g of Radix Pseudostellariae Pseudostellariae as raw material, crush it to a particle size of 40 mesh, add 7 times the volume of 95% industrial ethanol, and conduct heat reflux extraction at 85°C for 3 hours. After extraction, the extract is filtered out, and then The above process was repeated twice, the extracts were combined three times, concentrated under reduced pressure to a specific gravity of 1.15, and 66.00 g of ethanol extract was obtained, and the content of Pseudostellaria cyclic peptide B was 0.23%. Add 11 times the volume of 95% industrial ethanol to the extract and dissolve under reflux for 1.5h, cool and stand for 12h, filter, collect the filtrate, concentrate and reclaim the solvent to obtain 17.00g of the total cyclic peptide extract of Radix Pseudostellariae, the content of Pseudostellaria cyclic peptide B is 0.83%, the enrichment rate is 93.25%.

[0039] (2) Enrichment of Pseudostellaria cyclic peptide B by silica gel column chromatography...

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Abstract

The invention provides a preparation method of a standard radix pseudostellariae cyclopeptide B. The preparation method comprises the steps of extracting and separating standard radix pseudostellariae cyclopeptide B from traditional Chinese medicine radix pseudostellariae, and purifying standard radix pseudostellariae cyclopeptide B; smashing the dry medicinal material of radix pseudostellariae, and then, carrying out heat reflux extraction on the dry medicinal material of radix pseudostellariae by using industrial ethanol to obtain an extract; pretreating the extract by using ethanol; enriching radix pseudostellariae cyclopeptide B through a silica gel column chromatography, wherein a chloroform and methanol system is used as an eluting solvent; finally, preparing radix pseudostellariae cyclopeptide B with the purity of more than 98% by using a preparative high performance liquid chromatography. The method provided by the invention can be used for rapidly separating radix pseudostellariae cyclopeptide B and is low in sample loss, relatively low in cost, convenient to operate, good in controllability and repeatability and suitable for industrial production; in addition, the solvent can be repeatedly recycled.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an extraction, separation and purification method for preparing a standard product Pseudostellaria cyclic peptide B by using the traditional Chinese medicine Pseudostellaria Pseudostellariae as a raw material. Background technique [0002] Pseudostellariae Radix (‘Tai Zi Shen’) is the dry root tuber of Pseudostellaria heterophylla (Miq.) Pax ex Pax et Hoffm., a plant of the caryophyllaceae family. The currently commercially available Pseudostellaria mainly comes from planting bases in Anhui, Guizhou, Fujian, Jiangsu, and Shandong. Sweet, slightly bitter, flat, returns to the spleen and lung meridians, has the effects of nourishing qi and invigorating the spleen, promoting body fluid and moistening the lungs, etc. It is used for spleen deficiency, fatigue, loss of appetite, weakness after illness, deficiency of qi and yin, spontaneous sweating, thirst, dryness of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/64C07K1/16
Inventor 谭宁华赵丽梅宋卫武
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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