Two-dimensional preparative chromatography instrument system having online enrichment function, and applications thereof

A two-dimensional preparation and chromatographic system technology, applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems that the system does not have the enrichment function, is difficult to separate and monitor, and affects the separation effect, so as to avoid sample volume overload and avoid Effect of denaturation and interference reduction

Active Publication Date: 2015-06-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

The above systems only have a simple fraction transfer function, and the direct entry of large-volume fractions containing target components into the second-dimensional preparative chromatography system will seriously affect its separation ability. The amount of sample loaded is used to ensure the preparation throughput, and the trace components are difficult to separate and monitor under the condition of severe overload operation, which affects the separation effect

Method used

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  • Two-dimensional preparative chromatography instrument system having online enrichment function, and applications thereof
  • Two-dimensional preparative chromatography instrument system having online enrichment function, and applications thereof
  • Two-dimensional preparative chromatography instrument system having online enrichment function, and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1 A single loading is used for the two-dimensional preparation of 40% content components

[0040]This experiment intends to prepare 40% of the ingredients in natural plant extracts. The first-dimensional preparative chromatographic column and the trapping column are both 10um Chromatorex C18 filler preparative chromatographic columns, the second-dimensional preparative chromatographic column is a 5um Microsorb C18 filler preparative chromatographic column, the first-dimensional preparative chromatographic mobile phase is 60% methanol, and the second-dimensional Prepare the chromatographic mobile phase as 45% acetonitrile. The sample solution in the quantitative loop enters the trapping column after passing through the ten-way valve and the guard column. At the same time, water is input to reduce the elution capacity of the mobile phase, so that the target components remain in the trapping column. The first-dimension preparative chromatographic mobile phase is u...

Embodiment 2

[0041] Example 2 Multiple loadings are used for the two-dimensional preparation of 0.2% content components

[0042] This experiment intends to prepare 0.2% content ingredients in natural plant extracts. The first-dimension preparative chromatographic column is a 10um Chromatorex C18 packing preparative column, the trapping column is a 10um Calesil C18 packing chromatographic column, the second-dimensional preparative chromatographic column is a 5um Microsorb C18 packing preparative column, and the first-dimension preparative chromatographic mobile phase is 80 % methanol, the mobile phase of the second dimension preparative chromatography was 75% methanol. The sample solution in the quantitative loop enters the trapping column after passing through the ten-way valve and the guard column, and at the same time, water is input to reduce the elution capacity of the mobile phase, so that the target components remain in the trapping column. The first-dimension preparative chromatogr...

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Abstract

The present invention relates to a two-dimensional preparative chromatography instrument system having the online enrichment function, and applications thereof. The system comprises a chromatography flow channel switching valve, a chromatography column, a solvent pump and a detector. According to the present invention, a trapping column collects a first-dimensional chromatography effluent component through valve switching and mobile phase dilution, and the trapped component is introduced into a second-dimensional preparative column through valve switching and mobile phase elution so as to achieve two-dimensional chromatography separation; the instrument system can perform two-dimensional separation, and the sample and the one-dimensional separation component are effectively retained and enriched through the trapping column interface, such that the column efficiency loss is low, and the two-dimensional separation effect is ensured; and the instrument system can enlargedly perform multiple circulation separations and multidimensional separation, and the separation effect can not be decreased along with the increasing sample volume and the increasing separation frequency. In addition, the instrument system further has the function of the concentration recovery on the finally-separated product so as to simplify the sample drying process, such that the degeneration of the sample during the heating drying process is easily be avoided.

Description

technical field [0001] The invention relates to a set of two-dimensional preparative chromatography instrument system with online enrichment function. Background technique [0002] Highly active chemical components in natural medicines are one of the important sources of new drugs. With the deepening of research, a large number of high-content components have been isolated. Researchers have gradually turned to the separation of trace chemical components in natural medicines to find new ones. Element. Compared with high-content components, trace components in natural medicines are more susceptible to interference from other components during the separation process, and separation and monitoring are difficult. Traditional separation techniques use a large amount of raw materials and low separation efficiency. Therefore, efficient separation tools are required. [0003] Preparative chromatography has a powerful separation ability and is the main tool for the separation of natu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/08
Inventor 肖红斌高明哲邹晓伟王志鑫
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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