This invention discloses a method for preparing pet
stem cell exosomes, belonging to the field of animal
medicine technology. This invention treats stem cells with an inducing agent to secrete exosomes with high TFR expression on their surface. These exosomes specifically target the conserved region of the NS1
gene, a non-
structural protein of the
parvovirus family, allowing the internally loaded siRNA and
trefoil factor 3 to target and enter infected cells, directly inhibiting
viral replication at the
gene level to treat feline panleukopenia
virus (FPV) and
canine parvovirus infections and alleviate sequelae. This invention solves the problems of existing technologies, such as difficulty in targeting, lack of specific drugs, reliance on intensive supportive care leading to a short treatment window, inability to directly clear the
virus, risk of cross-infection, and potential long-term sequelae. It has the potential to be applied to the preparation of drugs for treating
canine parvovirus and feline panleukopenia.