38 results about "Feline parvovirus" patented technology

The invention discloses an application of a compound pulchinenoside B4 shown as a formula I or pharmaceutically acceptable salt thereof in the preparation of drugs for treating viral and / or bacterialdiseases. The compound pulchinenoside B4 has strong biological activity, and has excellent treating effects on endometritis, foot rot, feline parvovirus, canine parvovirus, canine distemper, canine renal failure and canine acute nephritis.

The invention relates to a composition for detecting feline calicivirus and feline parvovirus. The composition comprises primers and probes for detecting the feline calicivirus and the feline parvovirus respectively, wherein the primers include, for example,the sequences shown in SEQ ID NO.1 to SEQ ID NO.4, the probes include, for example,the sequences shown inSEQ ID NO.5 to SEQ ID NO.6; or, the primers include, for example, the sequences shown inSEQ ID NO.7 to SEQ ID NO10, the probes include, for example, the sequences shown inSEQ ID NO.11 to SEQ ID NO12. The invention further relates to a kit and application comprising the composition. The kit performs amplification reaction and product detection in a closed tube state, and false positivity due to contamination of amplification productsis avoided; the probes are higher in hybridization specificity and higher in sensitivity; and no follow-up treatment is after the PCR, operation is simpler and faster, safe and pollution-free effectsare realized, and the detection of both FCV and FPV viruses can be realized at the same time.

The invention discloses an FPV (feline parvovirus)-resistant cat-derived genetic engineering antibody, and belongs to the technical field of antibody engineering. The antibody comprises a mouse-derived variable region and a cat-derived constant region, the nucleotide sequence of a heavy chain variable region of the antibody is represented as SEQ ID NO.1, the gene sequence of a heavy chain constant region of the antibody is represented as SEQ ID NO.5, the nucleotide sequence of a light chain variable region of the antibody is represented as SEQ ID NO.2, and the gene sequence of a light chain constant region of the antibody is represented as SEQ ID NO.6. The variable region sequence of a monoclonal antibody of a canine parvovirus is assembled with the cat-derived constant region, and the FPV-resistant cat-derived genetic engineering antibody is obtained, shows good FPV neutralization activity, has an effect of inhibiting red cell agglutination of the FPV, can be applied to the fields of cat-derived research for a canine parvovirus monoclonal antibody and the like and has great significance for promoting development of the cat-derived monoclonal antibody drugs.

The invention provides feline panleukopenia virus (FPV) VP2 protein and prepared virus-like particles. The amino acid sequence of the VP2 protein is shown in SEQ ID NO: 2; the nucleotide sequence of an encoding gene of the VP2 protein is shown in SEQ ID NO: 1. In another aspect, the invention provides a recombinant baculovirus, and the recombinant baculovirus comprises nucleotide fragments encoding the VP2 protein; the constructed recombinant baculovirus is applied to preparation of the virus-like particles of the FPV in insect cells. The invention further provides an FPV subunit vaccine comprising antigens and vaccine adjuvants; the used antigens are the prepared virus-like particles provided by the invention. After being immune to a cat, the prepared vaccine provided by the invention canimprove the antibody titer of the cat, and can effectively prevent the infection of the FPV.

The invention is applicable to the technical field of feline parvovirus detection, and provides a primer, a probe, a reagent and a method for rapidly detecting feline parvoviruses at a normal temperature and a constant temperature. According to the invention, the feline parvoviruses can be rapidly and specifically detected in real time; under the combined action of a specific primer, a specific fluorescent probe, five engineering enzymes and chemical components, rapid detection of a nucleic acid target object is achieved in an instrument with a fluorescence detection function; meanwhile, closed tube detection is guaranteed through strict experiment operation steps; the aerosol pollution is effectively prevented; and the primer, probe, reagent and method provided by the invention are applicable to being applied to various fluorescence detection devices for detection.

The invention provides a recombinant feline parvovirus VP2 protein antigen and application thereof in vaccine preparation and virus diagnosis. A plurality of B cell antigens of the feline parvovirus VP2 protein are subjected to tandem expression by using a prokaryotic expression vector. The expressed recombinant protein is purified and used as a coating antigen for detecting the feline parvovirus antibody. And compared with a whole virus coating method in parallel, the values of detected positive and negative serum are highly consistent. The antigen treatment method provided by the is convenient, the test time is shortened, and the operation steps are simpler. According to the invention, an indirect ELISA method is established for detecting the antibody level of the feline parvovirus in feline serum, which has the characteristics of good repeatability and high specificity, and can be used for feline parvovirus serology investigation. Therefore, the indirect ELISA detection kit for the feline parvovirus based on the tandem expression of the VP2 protein B cell antigens, provided by the invention, is very suitable for the detection of clinical large samples and is suitable for large-scale popularization.

The invention discloses a fluorescent quantitative PCR detection primer for simultaneously detecting feline parvovirus and feline HIV, a probe and a kit . After the feline parvovirus NS1 gene sequenceand the feline HIV pol gene sequence are compared, it is found that the gene sequences of the feline parvovirus NS1 gene sequence and the feline HIV pol gene sequence do not intersect and are low inhomology. Therefore, a specific probe and a specific primer are designed according to the sequences of the feline parvovirus and the feline HIV, and the feline parvovirus and the feline HIV are diagnosed through fluorescent quantitative PCR amplification. The primer and the probe are prepared into a kit for simultaneously detecting the feline parvovirus and the feline AIDS virus. The whole processonly needs about 2 h, the time is short, the kit is easy to operate, high in sensitivity and good in specificity, the feline parvovirus and the feline AIDS virus can be detected at the same time, andomission of detection of one of the viruses can be avoided.

The invention discloses a cat parvovirus strain and application thereof, and belongs to the technical field of biological products. The preservation number of the strain is CCTCC NO: V202127, the preservation time is April 27, 2021, and the preservation address is Wuhan University, Wuhan City, China. The strain belongs to an isolated strain in Wuhan of China, and has high virus titer; an inactivated product of the virus can generate a good immune response reaction in animals, has good immunogenicity, can well stimulate the body to generate a high-level neutralizing antibody for FPV as an inactivated vaccine, and has the basic potential of vaccines. The vaccine prepared from the virus strain can be used for preventing and treating cat leukopenia, so that an important vaccine virus source is provided for prevention and control of the cat leukopenia in China, and effective prevention and control of cat parvovirus are realized.

The invention provides a feline panleukopenia virus antibody ELISA kit and a detection method for a feline panleukopenia virus antibody ELISA. The feline panleukopenia virus antibody ELISA kit comprises an enzyme-labeled plate and an enzyme-labeled antibody which are coated with antigens; the antigens are FPV purified antigens, and the enzyme-labeled antibody is a goat anti-feline IgG antibody labeled by horse radish peroxidase; and the feline panleukopenia virus antibody ELISA kit and the detection method for the feline panleukopenia virus antibody ELISA have very high sensibility, specificity and accuracy for the detection of the feline panleukopenia virus antibody.

The present invention discloses an HRM primer set for identifying canine parvovirus (CPV) and feline parvovirus (FPV), a kit containing the primer set, and applications of the kit, wherein the primerset comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is represented by SEQ ID NO.1, and the nucleotide sequence of the downstream primer is represented by SEQ ID NO.2. The present invention further provides an HRM analysis kit containing the primer set. According to the present invention, the experiment results prove that the kit has good sensitivity and good specificity in the detection of FPV and CPV, and has high coincidence, high relative specificity and high sensitivity compared to virus isolation, DNA sequencing and other tests; and thekit can provide the effective technical means for the prevention and control of diseases caused by canine parvovirus (CPV) and feline parvovirus (FPV).

The invention belongs to the technical field of bioengineering, and relates to a feline parvovirus recombinant protein which comprises two dominant epitopes of a FPV (feline parvovirus) antigen. In order to improve the yield of the recombinant protein in a prokaryotic expression system, the amino acid sequence of the recombinant protein is converted into the corresponding nucleotide sequence by the aid of Escherichia coli biased codons, the nucleotide sequence is chemically synthesized, and a recombinant expression vector is constructed. The invention further relates to a preparation method ofa monoclonal antibody of the feline parvovirus recombinant protein. According to the preparation method, immunization, cell fusion and multiple screening are implemented to obtain a hybridoma cell strain, the monoclonal antibody is purified, colloidal gold particles are marked, an optimal monoclonal antibody matched composition is determined through an orthogonal experiment, and the recombinant protein can be used for early diagnosis of feline parvovirus infection.

The invention belongs to the technical field of vaccine preparation, relates to a novel feline parvovirus vaccine, and discloses a coding gene of a feline parvovirus VP2 protein. According to the invention, an FPV VP2 protein sequence is coded and optimized through a genetic engineering technology, a recombinant plasmid is constructed, a recombinant protein is expressed by using escherichia coli, and the FPV VP2 protein is co-expressed with four molecular chaperones respectively; and a target protein is purified by different purification methods to obtain a virus-like particle which is similar to natural viruses in size and has good hemagglutination. A new thought and a new basis are provided for novel feline parvovirus vaccines.

The invention belongs to the technical field of biology, and discloses a serum-free full-suspension culture type F81 cell line as well as a construction method and application thereof. The cat kidney suspension cell line is named as a cat kidney cell F81 suspension cell, is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China on November 4, 2021, has a preservation number of CCTCC NO: C2021289, has been passed to 65 generations at present, and is mainly in a transparent round shape with uniform size; a virus sensitivity test shows that the serum-free full-suspension culture type F81 cell line is sensitive to feline parvovirus; the cell line can be used for culturing, separating and detecting viruses, preparing viral vaccines and screening drugs for preventing or treating diseases caused by virus infection.

The invention relates to a nucleic acid combination product, a detection kit and a micro-fluidic chip. The nucleic acid combination product comprises at least two of the following detection primer pairs: a feline parvovirus primer pair, a feline coronavirus primer pair, a feline herpesvirus type I primer pair, a feline calicivirus primer pair, a feline leukemia virus primer pair, a feline immunodeficiency virus primer pair, a feline astrovirus primer pair, a feline rotavirus primer pair, a cat vaccinia virus primer pair and a cat reovirus primer pair. The nucleic acid combination product can be used for detecting at least two feline pathogenic viruses in the sample to be detected at one time, and is good in specificity and relatively high in detection sensitivity.

The invention relates to the technical field of molecular biomedicine, in particular to a PCR (Polymerase Chain Reaction) detection kit for cat and / or dog pathogens, a detection method and application. The kit is used for detecting canine distemper virus, canine influenza A virus, canine parainfluenza virus, canine parvovirus, canine coronavirus, canine rotavirus, canine babesia, canine ascaris, canine Ehrlichia, canine brucella, rabies virus, borrelia burgdorferi, reference gene ACTB, feline herpes virus, feline calicivirus and feline parvovirus. The kit comprises primers and probes of feline coronavirus, feline immunodeficiency virus, feline leukemia virus, feline mycoplasma, feline mycoplasma, feline chlamydia, giardia, toxoplasma, bartonella and reference gene GAPDH, collected DNA and RNA are added into the kit, a real-time fluorescence PCR instrument is adopted for PCR reaction, FAM, HEX, ROX and CY5 fluorescence signals are collected in each cycle, analysis of related pathogens is carried out, and the kit can be used for detecting the feline and the canine. Compared with a traditional detection method, the method has the advantages of higher specificity and higher sensitivity.

The invention discloses an anti-FPV (feline parvovirus) feline genetic engineering antibody ELISA kit and a detection method thereof and belongs to the technical field of genetic engineering antibodyanalysis and detection. The kit comprises an ELISA plate coated by an antigen and an enzyme-labeled antibody, wherein the antigen is a FPV-VLPS purified antigen, and the enzyme-labeled antibody is a goat anti-cat IgG antibody labeled by horseradish peroxidase. The detection method comprises the following steps: coating the ELISA plate; drawing a standard curve; adding the goat anti-cat IgG antibody labeled by the horseradish peroxidase, carrying out TMB color developing and stop of a stop solution; measuring an OD450 value of each reaction hole; and performing judgment according to judgment standard and the like. The kit and the detection method have very high sensitivity, specificity and stability in detecting anti-FPV (feline parvovirus) feline genetic engineering antibodies, and providestrong support for study in detecting the level of the anti-FPV (feline parvovirus) feline genetic engineering antibodies in animals before and after emergency immunization.

The invention discloses a primer group, reagent and method for detecting feline parvovirus based on polymerase spiral reaction, the primer group comprises specific primers PSR-FPVF and PSR-FPVR, acceleration primers PSR-FPVF FJ and PSR-FPVF RJ, the nucleotide sequence of the specific primers PSR-FPVF is shown as SEQ ID NO.1, the nucleotide sequence of the specific primers PSR-FPVR is shown as SEQ ID NO.2, the nucleotide sequence of the acceleration primer PSR-FPVF FJ is as shown in SEQ ID NO. 3, and the nucleotide sequence of the acceleration primer PSR-FPVF RJ is as shown in SEQ ID NO. 4. The PSR detection method constructed by the primer group is simple to operate, has low requirements on detection instruments, greatly shortens the detection time, can be completed within 45 minutes at the constant temperature of 67 DEG C, and can judge the detection result by naked eyes under visible light by adding the nucleic acid dye with the final concentration of 20 * SYBR Green I into the system after the amplification is finished, thereby greatly improving the detection efficiency.

The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.

The invention provides a feline parvovirus VP2 protein and an obtained self-assembled virus-like particle, and belongs to the field of VLP vaccines. The cat parvovirus VP2 gene provided by the invention has a nucleotide sequence as shown in SEQ ID NO. 1. The DNA sequence of the cat parvovirus VP2 gene is artificially modified and synthesized according to the preference of codons of escherichia coli, and the VP2 protein expressed by using the sequence has good immunocompetence and can effectively protect pet cats and felines from being invaded by cat plague viruses.

The invention belongs to the technical field of virus antibodies, and discloses a feline panleukopenia virus antibody sequence, a tetrapeptide chain molecule, an immunoglobulin molecule and application, wherein the feline panleukopenia virus antibody sequence is a heavy chain variable region amino acid sequence SEQ ID NO: 1 and a nucleotide sequence SEQ ID NO: 3; and a light chain variable regionamino acid sequence SEQ ID NO: 2 and a nucleotide sequence SEQ ID NO: 4. A sequence screening method comprises the following steps of preparing a bacteriophage antibody; screening a bacteriophage antibody library; identifying an anti-feline panleukopenia virus feline-derived bacteriophage single-chain antibody by using a PhageELISA method; sending a scFv bacterial liquid with a positive PhageELISAidentification result to a sequencing company for sequencing to obtain variable region sequences of a heavy chain and a light chain of the feline-derived anti-feline panleukopenia virus genetic engineering antibody. The invention provides support for the construction of feline-derived anti-feline panleukopenia virus genetically engineered antibodies with high affinity and low immunogenicity. Themethod has important significance for promoting the development of feline-derived antibody drugs.

The invention discloses a method for rapidly detecting feline calicivirus as well as a primer and a kit for detection, and relates to a method for detecting the feline calicivirus as well as a primer and a kit for detection. According to the invention, an upstream primer ERA-F, a downstream primer ERA-R and a probe Probe are protected. The kit comprises the primer, the probe, a positive control sample, an isothermal nucleic acid amplification kit and a test strip. The detection method comprises the following steps: 1, preparing a detection solution; and 2, detecting a reaction product in the step 1 by using a test strip to obtain a detection result. According to the invention, the feline calicivirus is rapidly detected through an RT-ERA technology by adopting a primer and probe combination mode. The primer and probe combination disclosed by the invention is designed aiming at a feline calicivirus ORF1 conserved region, has the advantages of high sensitivity, strong specificity, good repeatability and the like, and has no cross reaction with feline herpes virus, feline parvovirus and feline infectious peritonitis virus.

The invention relates to the technical field of biology, in particular to a triple egg yolk antibody for cats, a preparation, a preparation method and application. Wherein the triple egg yolk antibody comprises a cat parvovirus egg yolk antibody, a cat herpes virus egg yolk antibody and a cat calicivirus egg yolk antibody. The triple egg yolk antibody provided by the invention can be used for prevention and adjuvant treatment of feline plague, feline nasal branch and feline infectious nasal conjunctivitis, and is high in protection rate and good in effect. Particularly, the traditional Chinese medicine composition has a good treatment effect or an auxiliary treatment effect on the feline plague diarrhea, and the treatment time of the feline plague diarrhea can be remarkably shortened. According to the invention, the treatment approach of the feline plague diarrhea is expanded, and a new direction is provided for the treatment of the feline plague diarrhea.

The invention discloses a hybridoma cell 3A6 strain capable of secreting an anti-feline parvovirus VP2 protein monoclonal antibody and an application of the hybridoma cell 3A6 strain, and belongs to the field of biology. The hybridoma cell 3A6 strain is preserved in China Center for Type Culture Collection on December 10, 2020, and the preservation number is CCTCC NO: C2020248. The hybridoma cell3A6 strain can react with eukaryotic expression VP2 protein and feline parvovirus, the biological performance is very excellent, the neutralizing titer of the prepared mouse ascites monoclonal antibody to virus is as high as 5 * 108, the reaction titer to VP2 protein is as high as 108, the neutralizing capacity to various feline parvovirus strains is equivalent, and the hybridoma cell 3A6 strain is used for clinical treatment of feline parvovirus infected cats, and the effective rate reaches 100%.

The invention belongs to the technical field of biology, and particularly relates to a primer probe set and a kit for detecting FPV through an RAA fluorescence method.The primer probe set can detect more FPV genotypes after degeneracy design, compared with other primer probe sets, it is found that the primer probe set has the advantages of being high in sensitivity and better in detection effect, and meanwhile the primer probe set can be used for detecting the FPV genotypes. The invention also prepares a corresponding RAA kit and establishes a corresponding RAA detection method, tests show that the kit can be carried out under a constant temperature condition (37-42 DEG C), can realize rapid and qualitative detection of the feline parvovirus within 5-30 min, has the characteristics of convenience in operation, strong specificity, high sensitivity and good repeatability, and can completely meet the requirements of rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus, rapid diagnosis of the feline parvovirus and rapid diagnosis of the feline parvovirus. The kit is suitable for clinical rapid detection and has a wide application prospect.

The invention relates to the technical field of disinfectants, in particular to a disinfectant for pets and a preparation method thereof. The disinfectant for pets comprises the following components in percentage by mass: 95-98.5% of acidic electrolyzed oxidizing water, 1-5% of a stabilizer and 0.1-1% of a strong oxidizing compound. The preparation method of the disinfectant for pets comprises the following steps: electrolyzing a sodium chloride solution by using a diaphragm-free electrolysis device, and generating acidic electrolyzed oxidizing water in an anode chamber; and adding the stabilizer and the strong oxidizing mixture in a certain proportion into acidic electrolyzed oxidizing water, and stirring and dissolving to obtain the disinfectant for pets. The disinfectant for pets prepared by the method is suitable for killing DNA viruses such as canine parvovirus and feline parvovirus and RNA viruses such as canine distemper and feline coronavirus; the pet disinfectant can be directly sprayed to disinfect pets, and is non-irritant to skin and non-toxic in mouth.

The invention discloses a primer set, a reagent and a method thereof for detecting feline parvovirus based on a polymerase helical reaction. The primer set includes specific primers PSR-FPVF and PSR-FPVR, accelerated primers PSR-FPVF FJ and PSR-FPVF RJ, the nucleotide sequence of the specific primer PSR-FPVF is shown in SEQ ID NO.1, the nucleotide sequence of the specific primer PSR-FPVR is shown in SEQ ID NO.2, and the nuclear sequence of the accelerated primer PSR-FPVF FJ The nucleotide sequence is shown in SEQ ID NO.3, and the nucleotide sequence of the accelerating primer PSR‑FPVF RJ is shown in SEQ ID NO.4. The PSR detection method constructed by this primer set is simple to operate, has low requirements for detection equipment, and greatly shortens the detection time. It can be completed within 45 minutes at a constant temperature of 67°C. After the amplification, a final concentration of 20×SYBR is added to the system. Green I nucleic acid dye, the detection result can be judged by naked eyes under visible light, which greatly improves the detection efficiency.

The invention discloses a cat parvovirus neutralizing monoclonal antibody and application thereof, and relates to the technical field of bioengineering. The invention provides a hybridoma cell strain which is preserved in China Center for Type Culture Collection on May 6, 2022, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: C2022111. The invention also provides a cat parvovirus neutralizing monoclonal antibody which is secreted by the hybridoma cell, and the specific sequences of the cat parvovirus neutralizing monoclonal antibody are shown as SEQ ID NO: 1 and SEQ ID NO: 2. The hybridoma cell can secrete and generate the cat parvovirus neutralizing monoclonal antibody, and the monoclonal antibody is proved to have very high neutralizing activity through in-vitro cellular level neutralization tests and animal protective experiments, can specifically treat FPV, is simple to prepare and convenient to operate, and has great significance for preventing and treating the FPV.

The invention discloses the use of a compound Pulsatilla saponin B4 represented by formula I or a pharmaceutically acceptable salt thereof in the preparation of medicines for treating viral and / or bacterial diseases. The compound Pulsatilla saponin B4 of the invention has strong biological activity, and has excellent therapeutic effects on endometritis, foot rot, feline parvovirus, canine parvovirus, canine distemper, canine kidney failure and canine acute nephritis.

The present invention relates to a vaccine for immunizing a cat against feline viruses. The present invention also relates to a nucleic acid clone that encodes the capsid protein of the isolated feline calicivirus. The present invention further relates to a live or killed vaccine comprising the isolated feline calicivirus, a subunit vaccine comprising the capsid protein of the isolated feline calicivirus, a nucleic acid vaccine comprising a nucleic acid clone of the isolated feline calicivirus, and a recombinant virus vector vaccine comprising nucleic acid encoding the capsid protein of the isolated feline calicivirus. The present invention also relates to a method for identifying a feline calicivirus useful for producing a vaccine composition and for assays for diagnosing cats infected with feline calicivirus. Also disclosed is a method of immunizing animals, especially cats, against disease, in particular against feline calicivirus (FCV). The method includes administering to a cat therapeutically effective amounts of first and second FCV vaccines. The first vaccine is administered orally or parenterally (e.g., subcutaneously, intramuscularly, and the like). The second vaccine is administered orally or oronasally N days following administration of the first vaccine, wherein N is an integer from 3 to 120, inclusive. A third vaccine administration may also be given. The present invention also describes methods and materials for treating and immunizing animals with vaccine, and in particular cats against both FPV or Feline Parvovirus, which has also been called Panleukopenia or FPL and against another disease, FHV or Feline Herpes Virus, which has also been called Feline Rhinotracheitis Virus.