37 results about "Feline parvovirus" patented technology

The invention relates to a composition for detecting feline calicivirus and feline parvovirus. The composition comprises primers and probes for detecting the feline calicivirus and the feline parvovirus respectively, wherein the primers include, for example,the sequences shown in SEQ ID NO.1 to SEQ ID NO.4, the probes include, for example,the sequences shown inSEQ ID NO.5 to SEQ ID NO.6; or, the primers include, for example, the sequences shown inSEQ ID NO.7 to SEQ ID NO10, the probes include, for example, the sequences shown inSEQ ID NO.11 to SEQ ID NO12. The invention further relates to a kit and application comprising the composition. The kit performs amplification reaction and product detection in a closed tube state, and false positivity due to contamination of amplification productsis avoided; the probes are higher in hybridization specificity and higher in sensitivity; and no follow-up treatment is after the PCR, operation is simpler and faster, safe and pollution-free effectsare realized, and the detection of both FCV and FPV viruses can be realized at the same time.

The invention discloses an FPV (feline parvovirus)-resistant cat-derived genetic engineering antibody, and belongs to the technical field of antibody engineering. The antibody comprises a mouse-derived variable region and a cat-derived constant region, the nucleotide sequence of a heavy chain variable region of the antibody is represented as SEQ ID NO.1, the gene sequence of a heavy chain constant region of the antibody is represented as SEQ ID NO.5, the nucleotide sequence of a light chain variable region of the antibody is represented as SEQ ID NO.2, and the gene sequence of a light chain constant region of the antibody is represented as SEQ ID NO.6. The variable region sequence of a monoclonal antibody of a canine parvovirus is assembled with the cat-derived constant region, and the FPV-resistant cat-derived genetic engineering antibody is obtained, shows good FPV neutralization activity, has an effect of inhibiting red cell agglutination of the FPV, can be applied to the fields of cat-derived research for a canine parvovirus monoclonal antibody and the like and has great significance for promoting development of the cat-derived monoclonal antibody drugs.

The invention provides feline panleukopenia virus (FPV) VP2 protein and prepared virus-like particles. The amino acid sequence of the VP2 protein is shown in SEQ ID NO: 2; the nucleotide sequence of an encoding gene of the VP2 protein is shown in SEQ ID NO: 1. In another aspect, the invention provides a recombinant baculovirus, and the recombinant baculovirus comprises nucleotide fragments encoding the VP2 protein; the constructed recombinant baculovirus is applied to preparation of the virus-like particles of the FPV in insect cells. The invention further provides an FPV subunit vaccine comprising antigens and vaccine adjuvants; the used antigens are the prepared virus-like particles provided by the invention. After being immune to a cat, the prepared vaccine provided by the invention canimprove the antibody titer of the cat, and can effectively prevent the infection of the FPV.

The invention is applicable to the technical field of feline parvovirus detection, and provides a primer, a probe, a reagent and a method for rapidly detecting feline parvoviruses at a normal temperature and a constant temperature. According to the invention, the feline parvoviruses can be rapidly and specifically detected in real time; under the combined action of a specific primer, a specific fluorescent probe, five engineering enzymes and chemical components, rapid detection of a nucleic acid target object is achieved in an instrument with a fluorescence detection function; meanwhile, closed tube detection is guaranteed through strict experiment operation steps; the aerosol pollution is effectively prevented; and the primer, probe, reagent and method provided by the invention are applicable to being applied to various fluorescence detection devices for detection.

The invention discloses a fluorescent quantitative PCR detection primer for simultaneously detecting feline parvovirus and feline HIV, a probe and a kit . After the feline parvovirus NS1 gene sequenceand the feline HIV pol gene sequence are compared, it is found that the gene sequences of the feline parvovirus NS1 gene sequence and the feline HIV pol gene sequence do not intersect and are low inhomology. Therefore, a specific probe and a specific primer are designed according to the sequences of the feline parvovirus and the feline HIV, and the feline parvovirus and the feline HIV are diagnosed through fluorescent quantitative PCR amplification. The primer and the probe are prepared into a kit for simultaneously detecting the feline parvovirus and the feline AIDS virus. The whole processonly needs about 2 h, the time is short, the kit is easy to operate, high in sensitivity and good in specificity, the feline parvovirus and the feline AIDS virus can be detected at the same time, andomission of detection of one of the viruses can be avoided.

The invention discloses a cat parvovirus strain and application thereof, and belongs to the technical field of biological products. The preservation number of the strain is CCTCC NO: V202127, the preservation time is April 27, 2021, and the preservation address is Wuhan University, Wuhan City, China. The strain belongs to an isolated strain in Wuhan of China, and has high virus titer; an inactivated product of the virus can generate a good immune response reaction in animals, has good immunogenicity, can well stimulate the body to generate a high-level neutralizing antibody for FPV as an inactivated vaccine, and has the basic potential of vaccines. The vaccine prepared from the virus strain can be used for preventing and treating cat leukopenia, so that an important vaccine virus source is provided for prevention and control of the cat leukopenia in China, and effective prevention and control of cat parvovirus are realized.

The present invention discloses an HRM primer set for identifying canine parvovirus (CPV) and feline parvovirus (FPV), a kit containing the primer set, and applications of the kit, wherein the primerset comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is represented by SEQ ID NO.1, and the nucleotide sequence of the downstream primer is represented by SEQ ID NO.2. The present invention further provides an HRM analysis kit containing the primer set. According to the present invention, the experiment results prove that the kit has good sensitivity and good specificity in the detection of FPV and CPV, and has high coincidence, high relative specificity and high sensitivity compared to virus isolation, DNA sequencing and other tests; and thekit can provide the effective technical means for the prevention and control of diseases caused by canine parvovirus (CPV) and feline parvovirus (FPV).

The invention belongs to the technical field of bioengineering, and relates to a feline parvovirus recombinant protein which comprises two dominant epitopes of a FPV (feline parvovirus) antigen. In order to improve the yield of the recombinant protein in a prokaryotic expression system, the amino acid sequence of the recombinant protein is converted into the corresponding nucleotide sequence by the aid of Escherichia coli biased codons, the nucleotide sequence is chemically synthesized, and a recombinant expression vector is constructed. The invention further relates to a preparation method ofa monoclonal antibody of the feline parvovirus recombinant protein. According to the preparation method, immunization, cell fusion and multiple screening are implemented to obtain a hybridoma cell strain, the monoclonal antibody is purified, colloidal gold particles are marked, an optimal monoclonal antibody matched composition is determined through an orthogonal experiment, and the recombinant protein can be used for early diagnosis of feline parvovirus infection.

The invention belongs to the technical field of biology, and discloses a serum-free full-suspension culture type F81 cell line as well as a construction method and application thereof. The cat kidney suspension cell line is named as a cat kidney cell F81 suspension cell, is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China on November 4, 2021, has a preservation number of CCTCC NO: C2021289, has been passed to 65 generations at present, and is mainly in a transparent round shape with uniform size; a virus sensitivity test shows that the serum-free full-suspension culture type F81 cell line is sensitive to feline parvovirus; the cell line can be used for culturing, separating and detecting viruses, preparing viral vaccines and screening drugs for preventing or treating diseases caused by virus infection.

The invention relates to a nucleic acid combination product, a detection kit and a micro-fluidic chip. The nucleic acid combination product comprises at least two of the following detection primer pairs: a feline parvovirus primer pair, a feline coronavirus primer pair, a feline herpesvirus type I primer pair, a feline calicivirus primer pair, a feline leukemia virus primer pair, a feline immunodeficiency virus primer pair, a feline astrovirus primer pair, a feline rotavirus primer pair, a cat vaccinia virus primer pair and a cat reovirus primer pair. The nucleic acid combination product can be used for detecting at least two feline pathogenic viruses in the sample to be detected at one time, and is good in specificity and relatively high in detection sensitivity.

The invention discloses a primer group, reagent and method for detecting feline parvovirus based on polymerase spiral reaction, the primer group comprises specific primers PSR-FPVF and PSR-FPVR, acceleration primers PSR-FPVF FJ and PSR-FPVF RJ, the nucleotide sequence of the specific primers PSR-FPVF is shown as SEQ ID NO.1, the nucleotide sequence of the specific primers PSR-FPVR is shown as SEQ ID NO.2, the nucleotide sequence of the acceleration primer PSR-FPVF FJ is as shown in SEQ ID NO. 3, and the nucleotide sequence of the acceleration primer PSR-FPVF RJ is as shown in SEQ ID NO. 4. The PSR detection method constructed by the primer group is simple to operate, has low requirements on detection instruments, greatly shortens the detection time, can be completed within 45 minutes at the constant temperature of 67 DEG C, and can judge the detection result by naked eyes under visible light by adding the nucleic acid dye with the final concentration of 20 * SYBR Green I into the system after the amplification is finished, thereby greatly improving the detection efficiency.

The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.

The invention provides a feline parvovirus VP2 protein and an obtained self-assembled virus-like particle, and belongs to the field of VLP vaccines. The cat parvovirus VP2 gene provided by the invention has a nucleotide sequence as shown in SEQ ID NO. 1. The DNA sequence of the cat parvovirus VP2 gene is artificially modified and synthesized according to the preference of codons of escherichia coli, and the VP2 protein expressed by using the sequence has good immunocompetence and can effectively protect pet cats and felines from being invaded by cat plague viruses.

The invention discloses a method for rapidly detecting feline calicivirus as well as a primer and a kit for detection, and relates to a method for detecting the feline calicivirus as well as a primer and a kit for detection. According to the invention, an upstream primer ERA-F, a downstream primer ERA-R and a probe Probe are protected. The kit comprises the primer, the probe, a positive control sample, an isothermal nucleic acid amplification kit and a test strip. The detection method comprises the following steps: 1, preparing a detection solution; and 2, detecting a reaction product in the step 1 by using a test strip to obtain a detection result. According to the invention, the feline calicivirus is rapidly detected through an RT-ERA technology by adopting a primer and probe combination mode. The primer and probe combination disclosed by the invention is designed aiming at a feline calicivirus ORF1 conserved region, has the advantages of high sensitivity, strong specificity, good repeatability and the like, and has no cross reaction with feline herpes virus, feline parvovirus and feline infectious peritonitis virus.

The invention relates to the technical field of biology, in particular to a triple egg yolk antibody for cats, a preparation, a preparation method and application. Wherein the triple egg yolk antibody comprises a cat parvovirus egg yolk antibody, a cat herpes virus egg yolk antibody and a cat calicivirus egg yolk antibody. The triple egg yolk antibody provided by the invention can be used for prevention and adjuvant treatment of feline plague, feline nasal branch and feline infectious nasal conjunctivitis, and is high in protection rate and good in effect. Particularly, the traditional Chinese medicine composition has a good treatment effect or an auxiliary treatment effect on the feline plague diarrhea, and the treatment time of the feline plague diarrhea can be remarkably shortened. According to the invention, the treatment approach of the feline plague diarrhea is expanded, and a new direction is provided for the treatment of the feline plague diarrhea.

The invention discloses a hybridoma cell 3A6 strain capable of secreting an anti-feline parvovirus VP2 protein monoclonal antibody and an application of the hybridoma cell 3A6 strain, and belongs to the field of biology. The hybridoma cell 3A6 strain is preserved in China Center for Type Culture Collection on December 10, 2020, and the preservation number is CCTCC NO: C2020248. The hybridoma cell3A6 strain can react with eukaryotic expression VP2 protein and feline parvovirus, the biological performance is very excellent, the neutralizing titer of the prepared mouse ascites monoclonal antibody to virus is as high as 5 * 108, the reaction titer to VP2 protein is as high as 108, the neutralizing capacity to various feline parvovirus strains is equivalent, and the hybridoma cell 3A6 strain is used for clinical treatment of feline parvovirus infected cats, and the effective rate reaches 100%.

The invention relates to the technical field of disinfectants, in particular to a disinfectant for pets and a preparation method thereof. The disinfectant for pets comprises the following components in percentage by mass: 95-98.5% of acidic electrolyzed oxidizing water, 1-5% of a stabilizer and 0.1-1% of a strong oxidizing compound. The preparation method of the disinfectant for pets comprises the following steps: electrolyzing a sodium chloride solution by using a diaphragm-free electrolysis device, and generating acidic electrolyzed oxidizing water in an anode chamber; and adding the stabilizer and the strong oxidizing mixture in a certain proportion into acidic electrolyzed oxidizing water, and stirring and dissolving to obtain the disinfectant for pets. The disinfectant for pets prepared by the method is suitable for killing DNA viruses such as canine parvovirus and feline parvovirus and RNA viruses such as canine distemper and feline coronavirus; the pet disinfectant can be directly sprayed to disinfect pets, and is non-irritant to skin and non-toxic in mouth.

The invention discloses a cat parvovirus-like particle and a preparation method and application thereof.A VP2 sequence of an autonomously separated FPV JL-125 strain is selected for preparing a recombinant baculovirus FPV-VP2 strain, the FPV JL-125 strain and a current Chinese prevalent strain have high homology, after the VP2 sequence is optimized according to insect cell codons, the VP2 sequence is converted into a recombinant baculovirus FPV-VP2 strain, and the recombinant baculovirus FPV-VP2 strain is converted into a recombinant baculovirus FPV-VP2 strain. The prepared recombinant baculovirus FPV-VP2 strain can be used for correctly expressing the FPVVP2 protein. According to the invention, a full suspension culture process is adopted for culture, the expression quantity is greatly improved, and the HA titer can reach 220-221, which is 256-512 times of the culture titer of wild viruses. The expressed protein can be autonomously assembled into complete FPV virus-like particles, the space structure of the protein is similar to that of an original virus, and the virus-like particles are high in titer and higher in safety and have the advantages of stimulating humoral immunity and cellular immunity at the same time. According to the invention, the virus-like particle antigen is prepared by using a genetic engineering means, the novel cat parvovirus-like particle vaccine is prepared, and the vaccine has higher safety and effectiveness.