52 results about "Neutralization test" patented technology

The invention belongs to the field of molecular biology, and relates to a hybridoma cell strain capable of secreting a CPIV3 antibody and an ELISA kit. A caprine parainfluenza virus 3 CPIV3 immunogen used in the invention is a CPIV3 JS2013 viral strain isolated clinically, and a hybridoma cell strain 2E6 is screened from an established hybridoma cell bank capable of secreting the CPIV3 antibody. The monoclonal antibody secreted by the hybridoma cell strain has the blocking effect, and is applied to the CPIV3 blocking ELISA kit. The CPIV3 blocking ELISA kit can specifically detect the antibody generated after the CPIV3 infection or caprine immunization. 212 caprine clinical serum samples are colleted, and the CPIV3 antibody in the serum is tested by the established blocking ELISA method and a neutralization test. Through the comparison between the result of the blocking ELISA method and the result of the neutralization test, the coincidence rate is 98.8%.

The invention discloses an on-line sample dilution and neutralization test device which consists of a dilution device, a neutralizer, an instruction control device, a silica gel pipe and a control line, wherein an ultrapure water storage bottle and a sample bottle are connected to a peristaltic pump, an injection pump, a three-way mixer, an electric two-position four-way valve, a three-way mixer and the neutralizer through the dilution silica gel pipe; a control box is connected with the peristaltic pump, the injection pump, the three-way mixer, the electric two-position four-way valve and the neutralizer through the control main line in a control manner; automatic sample dilution and sample neutralization are completed on line through a pipeline and a circuit; samples are provided for a chromatographic analysis system to complete analysis. According to the on-line sample dilution and neutralization test device, the problem caused by ion pollution of an existing neutralization method is effectively solved; no suction pipe or no volumetric flask is used in the whole neutralization process any more, so that the pollution source is reduced, a precise value of experimental data measurement is guaranteed, and a reliable test condition is provided for research on ion chromatographic analysis of snow and ice samples.

The invention discloses a porcine circovirus-resistant egg yolk antibody and a preparation method and application thereof. The porcine circovirus-resistant egg yolk antibody is prepared by the following steps of: (1) immunizing a nonimmune egg-laying hen by using porcine circoviruses; (2) collecting eggs which laid by the nonimmune egg-laying hen; and (3) extracting the egg yolk antibody from the collected eggs and purifying. The level of the porcine circovirus-resistant egg yolk antibody prepared by the method is high; the expression of the egg yolk antibody is continuous, stable and small in fluctuation; and the titer of the egg yolk antibody is up to 1:640, and can be maintained for over 8 weeks. In-vitro and in-vivo neutralization tests prove that the porcine circovirus-resistant egg yolk antibody prepared by the method can effectively inhibit the porcine circoviruses, has a good immune protective effect, and can be prepared into a medicine or a preparation for preventing or treating diseases caused by the porcine circoviruses.

The invention relates to a method for preparing a porcine circovirus type 2 colloidal gold antibody fast test strip. In the method, a porcine circovirus type 2 ORF2 protein is expressed by utilizing genetic engineering, and the test strip is prepared by the principle of enzyme-linked immunoassay and membrane chromatography to fast test antibodies in the blood or serum of pigs. The test strip can be widely used for clinically testing porcine circovirus diseases, has no cross reaction with other viruses, and obtains test results 98.63 percent and 95.83 percent consistent with those obtained by the two methods of the ELISA and neutralization test. Compared with the ELISA, a recombinant antigen immune colloidal gold has the remarkable advantages of high security, no need of culturing the viruses per se, the avoidance of virus spread caused by the operation of the viruses, large-batch preparation, simple process, low production cost, stable and homogeneous antigen components, simple, convenient and labor-saving operation, no apparatus, high detection result specificity, high repeatability, the short time of 15 minutes for the whole test, simple, convenient, fast and accurate operation, high sensitivity, intuition and easy result judgment.

The invention provides a new hepatitis A virus JS-4 strain for preparing hepatitis A inactivated vaccine and a method for culturing the same. The strain is separated from deject a of a patient infected with acute hepatitis A and is transmitted to a Vero cell for adapted culture; through neutralization tests and other methods, the strain is proved to be hepatitis A virus; in the adaptation process, the subculture cycle of early generation is 28 days; when the strain is transmitted to the tenth generation, the cycle is shortened to 21 days; the strain is continuously cultured for ten generations; the culture temperature is between 35 and 36 DEG C; the cycle for virus propagation is shortened from 28 to 21 days; the antigen titer can reach 1:640-1:1,280; and the infectious titer is between 106.67 and 107.50 CCID50 / mL. The marmoset virulence test proves that the strain has weak virulence, is reliable in safety and has good immunogenicity and protective effect when the strain is used for producing the hepatitis A inactivated vaccine and is an ideal strain for producing the hepatitis A inactivated vaccine.

The invention relates to the field of animal virology and provides a chicken Newcastle disease virus and its separation method. The virus is code-named SDM01 and the invention provides its separation and preparation method. The inventor carries out biological collection for the strain and its collection number is CCTCC NO:V201109. Through chicken embryo passage, biological characteristics tests such as a hemagglutinin and serum neutralization test, an animal regress test and exogenous virus testing, are carried out. The test results confirm that the virus is the Newcastle disease virus. It isproved through chicken embryo mean death time (MDT), one-day-old chick intracebral pathogenicity index (ICPI) measurement and 6-week-old chick intravenous inoculation pathogenicity index (IVPI) measurement that the Newcastle disease virus is a virulent strain. Positive serum is prepared through separated strain and standard strain to carry out an HI cross neutralization test, a chicken embryo neutralization test, a cell neutralization test, an F and HN gene sequence sequencing and comparison and an immunity protective test. Results confirm that there exist large differences between the Newcastle disease virus SDM01 strain and traditional strains (La Sota strain and Clone30 strain) in both genetic typing and antigenicity.