Method for preparing porcine circovirus type 2 colloidal gold antibody fast test strip

Abstract

The invention relates to a method for preparing a porcine circovirus type 2 colloidal gold antibody fast test strip. In the method, a porcine circovirus type 2 ORF2 protein is expressed by utilizing genetic engineering, and the test strip is prepared by the principle of enzyme-linked immunoassay and membrane chromatography to fast test antibodies in the blood or serum of pigs. The test strip can be widely used for clinically testing porcine circovirus diseases, has no cross reaction with other viruses, and obtains test results 98.63 percent and 95.83 percent consistent with those obtained by the two methods of the ELISA and neutralization test. Compared with the ELISA, a recombinant antigen immune colloidal gold has the remarkable advantages of high security, no need of culturing the viruses per se, the avoidance of virus spread caused by the operation of the viruses, large-batch preparation, simple process, low production cost, stable and homogeneous antigen components, simple, convenient and labor-saving operation, no apparatus, high detection result specificity, high repeatability, the short time of 15 minutes for the whole test, simple, convenient, fast and accurate operation, high sensitivity, intuition and easy result judgment.

Description

technical field [0001] The invention relates to a diagnostic technique in the field of veterinary biotechnology, in particular to a preparation method for a rapid detection test strip of porcine circovirus type 2 colloidal gold antibody. Background technique [0002] Porcine circovirus 2 (PCV2) is the causative agent of post-weaning multisystemic wasting syndrome (PMWS) in weaned pigs, and has been widely recognized. With the in-depth research on PCV2 and its pathogenicity, it has recently been found that PCV2 is also associated with reproductive disorders in pigs, respiratory diseases in weaned pigs and fattening pigs, dermatitis and nephrotic syndrome (porcine dermatis and nephropathy syndrome, PDNS), young piglets diseases such as congenital tremor. In recent years, PCV2 infection in large-scale pig farms in my country and the diseases caused by it have become more and more common, causing large economic losses to pig production in my country. [0003] At present, the k...

AI Technical Summary

Problems solved by technology

However, the antigen preparation process of this method must go through cumbersome modes such as virus cell culture and virus purification. The specificity is not high, the stability is poor, and the cost is high; the detection requires special equipment such as a microplate reader. Personnel need to undergo professional training; the operation process is relatively complicated; the time required for detection is relatively long; the cost of detection is high

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