111 results about "Immunosorbents" patented technology

The invention relates to an integrated method for enriching and detecting a rare cell in a biological fluid sample. The enriching method comprises the following steps: (a) removing plasma protein by centrifugation; (b) optionally adding hematid lysis solution to carry out hematid lysis so as to remove hematid; (c) adding an immune microsphere or immune adsorbent to incubate; and (d) carrying out density centrifugation based on a specific cell separation medium so as to separate the a cycling rare cell, the residual cell after the hematid is removed and leucocyte combined on the immune microsphere. The method for detecting the enriched rare cells comprises the steps of combining pigmentation based on immune histochemistry or bi-color, tri-color or multicolor pigmentation based on the immunehistochemistry with immune fluorescence and observing and identifying by a fluorescent or common optical microscope or a scanner based on a microscope principle. The novel and unique method for enriching and detecting a rare cell in a biological fluid sample is proved to have low cost and can rapidly, effectively and highly specifically enrich and quantitatively detect the rare cell in blood.

The invention relates to an aflatoxin nano-antibody immunosorbent, an aflatoxin nano-antibody immunoaffinity column and preparation methods and application of the two. The immunosorbent comprises a solid phase carrier and an aflatoxin B1 nano-antibody 2014AFB-G15 coupled with the solid phase carrier, the half maximal inhibitory concentration (IC50) of the aflatoxin B1 nano-antibody 2014AFB-G15 to aflatoxin B1 is 0.66ng / mL, and the cross reaction rates of the aflatoxin B1 nano-antibody 2014AFB-G15 to aflatoxins B2, G1, G2 and M1 are 22.6%, 10.95%, 32.1% and 26%, respectively; an amino acid sequence of the aflatoxin B1 nano-antibody 2014AFB-G15 is represented by SEQIDNO:7, and a coding gene sequence of the aflatoxin B1 nano-antibody 2014AFB-G15 is represented by SEQIDNO:8. The aflatoxin nano-antibody immunoaffinity column disclosed by the invention can be used for purifying and concentrating a sample extracting solution before machinery detection and can be repeatedly used.

Immunosorbents, kits and compositions for diagnosing a central nervous system disorder, particularly paroxysmal cerebral discharges and epilepsy, comprising measuring the concentration of GluR1 or fragment thereof and / or GluR1 antibodies in a biological sample from a human subject. The method is particularly useful for identifying individuals that are at risk for brain related seizures and epilepsy, for distinguishing epilepsy from pseudo-epilepsy and epilepsy-like disorders, for following up after anticonvulsive treatment, and for the adjustment of adequate therapy and doses.