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Affinity absorption column for specially removing virions from blood and preparing method thereof

A virus particle and adsorption column technology, applied in the field of affinity adsorption column, can solve the problems of long treatment course, high cost, and large side effects, and achieve remarkable effects, low cost, and low toxicity

Inactive Publication Date: 2010-01-20
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current treatment methods for viral liver diseases include antiviral drugs and interferon, but the cost is high, the course of treatment is long, and the side effects are large

Method used

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  • Affinity absorption column for specially removing virions from blood and preparing method thereof

Examples

Experimental program
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Effect test

example 1

[0050] 1. Example 1: Monoclonal Antibody Immunoaffinity Adsorption Column Clears Hepatitis B Virus Surface Antigen in Plasma

[0051] 1. Reagent composition

[0052] ①(1,4)-2-amino-2-deoxy-β-D-glucose cross-linked resin (abbreviation: chitosan)

[0053] Take 1L of liquid paraffin containing 1% emulsifier (Span80) as an oil phase dispensing agent and place it in a three-necked bottle, add the pre-prepared 3% pore-forming agent (polyvinyl alcohol, polyethylene glycol 4000, ethyl acetate, ethyl acetate or butyl acetate) and 0.5L chitosan acetic acid solution of 3.5% polymer surface modifier (sodium dodecylphenyl sulfonate), stirred at room temperature for 1h, so that the chitosan solution was dispersed into a suitable size The temperature was raised to 40° C., and 0.2 L of pentanediol was added. After reacting for 0.5 h, the pH of the system was adjusted to 9 with 5% NaOH. Heat up to 60°C, react for 3 hours, filter, wash with water, extract with petroleum ether, wash with ethan...

example 2

[0073] 2. Example 2: Monoclonal Antibody Immunoaffinity Adsorbent Eliminates Human Immunodeficiency Virus (HIV) in Plasma

[0074] 1. Reagent composition

[0075] ① Carrier (same as above)

[0076] ②Activator (same as above)

[0077] ③ Monoclonal antibody

[0078] Anti-human immunodeficiency virus surface protein antibody can be obtained by monoclonal method.

[0079] ④ Immunosorbent

[0080] Add 3mL, 0.1mol / L, pH=9.6 buffer solution and 1g anti-human immunodeficiency virus surface protein GP120 antibody body to 1g of activated carrier, react at 0-4°C for 24h, use a large amount of 0.2mpl / L, pH=7.4 Slowly wash the resin with phosphate until the absorbance at 224nm is 0 to obtain the immunosorbent.

[0081] ⑤ Affinity adsorption process

[0082] The affinity adsorption device is the same as the affinity adsorption device in Example 1, only the adsorbent in the affinity adsorption column is replaced by the adsorbent coupled with the specific antibody against the surface pr...

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PUM

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Abstract

The invention discloses an affinity absorption column for specially removing virions from blood and preparing method thereof, in particular relates to an affinity absorption column for blood purification. The affinity absorption column comprises an absorption carrier, an activator and antivirus surface protein antibody; the activator is added into the absorption carrier to obtain an activated carrier; the antivirus surface protein antibody is added into the activated carrier to perform antibody coupling to obtain an immune absorber, and the immune absorber is loaded into the absorption column to obtain the affinity absorption column. The affinity absorption column has the advantages of strong specificity, little toxic side effect, good therapeutic effect, good repeatability and low cost, and is suitable for blood purification; besides, being a separation technology, the affinity absorption column can be widely applied to other fields for the purification and separation of virus antibodies.

Description

technical field [0001] The invention relates to an affinity adsorption column for blood purification, in particular to an affinity adsorption column for specifically removing virus particles in blood and a preparation method thereof, which can assist or replace traditional treatment methods and become a new type of virus treatment method of disease. Background technique [0002] Viral diseases are common infectious diseases, such as hepatitis B, which is a common viral infectious disease that currently plagues people. Viral hepatitis is a systemic acute or persistent infectious disease mainly caused by liver damage caused by a variety of hepatitis viruses. my country is a high-incidence area of ​​hepatitis B, the total infection rate of the population is as high as 60%, and the surface antigen carriers constituting the source of hepatitis B infection are about 10%. There are at least 120 million hepatitis B surface antigen carriers nationwide, about 10% of whom eventually ...

Claims

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Application Information

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IPC IPC(8): A61M1/38B01D15/08
Inventor 王业富李慧
Owner WUHAN UNIV
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