433 results about "Enteritides" patented technology

Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum. Further, the present invention relates to a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella Enteritidis or Salmonella Typhimurium, Fowl Typhoid caused by Salmonella Gallinarum, and Pullorum disease caused by Salmonella Pullorum, comprising the bacteriophage as an active ingredient. Furthermore, the present invention relates to a feed additive, drinking water, a cleaner and a sanitizer, comprising the bacteriophage as an active ingredient.

The invention discloses a multiple PCR identification kit of salmonella and five serotypes of salmonella. The invention provides a primer group for detecting salmonella and five serotypes of salmonella. The primer group comprises a primer pair 1, a primer pair 2, a primer pair 3, a primer pair 4, a primer pair 5 and a primer pair 6. Aiming at specific fragments of salmonella and its five important serotypes (such as salmonella typhimurium, enteritidis, agona, choleraesuis and pullorum), the six pairs of the primers for specific amplification are designed, the amplified fragments comprise 425bp of salmonella, 198bp of salmonella typhimurium, 302bp of salmonella enteritidis, 145bp of salmonella agona, 368bp of salmonella choleraesuis and 249bp of salmonella pullorum, and a sample gene group can be accordingly determined by multiple PCR and electrophoresis detection. Through six pairs of the specific primers and one-step multiple PCR, the salmonella and its five important serotypes can be identified. The multiple PCR identification kit has the characteristics of fast identification rate, low cost, easy operation and easy result determination.

Vaccines comprising fliC and CD 154 polypeptides and Salmonella enteritidis vaccine vectors comprising fliC polypeptides are provided. Also provided arc methods of enhancing an immune response against flagellated bacteria and methods of reducing morbidity associated with infection with flagellated bacteria.

The invention discloses a traditional Chinese medicine composition for preventing and treating bacterial diarrhea of livestock and poultry, and belongs to the field of traditional Chinese medicines. The traditional Chinese medicine composition comprises the following components in percentage by weight: 18-19% of coptis root, 14-15% of nutgall, 14-15% of pomegranate rind, 14-15% of myrobalam, 22-23% of astragalus mongholicus, 7-8% of atractylis ovata and 7-8% of radix sileris. The traditional Chinese medicine composition for preventing and treating bacterial diarrhea of livestock and poultry disclosed by the invention has significant curative effects on treating colibacillosis, salmonellosis and other bacterial diarrhea as well as chronic enteritis, irritable colon and other chronic diarrhea of the livestock and poultry. At the same time, the traditional Chinese medicine composition disclosed by the invention has the advantages of low production cost, and small toxic or side effect.

The invention relates to a medicine feed used for fish, in particular to a traditional Chinese medicine feed additive for preventing a fish disease, wherein the traditional Chinese medicine feed additive for preventing the fish disease is suitable for being used for curing and preventing the fish disease. The traditional Chinese medicine feed additive for preventing the fish disease is prepared by, by weight, 10-20 parts of rheum officinale, 5-15 parts of goldthread, 20-40 parts of Chinese gall, 20-30 parts of acorus calamus, 20-30 parts of common andrographis herb, and 20-40 parts of pericarpium granati. The traditional Chinese medicine feed additive for preventing the fish disease can be produced by being mixed with common fish feed and can be digested by fish, and the fish can be prevented from the disease and treated in a shortest time, and disease curing and preventing effect is good. The traditional Chinese medicine feed additive for preventing the fish disease is simple in production technique, reasonable in price, low in cost, wide in antibacterial spectrum, good in functions of insect killing and bacteriostatic action, pretty good in preventing and curing fish diseases such as bacillary enteritis, convenient to use, easy to store, good in pesticide effect, and good in economic benefit and social benefit.

The invention relates to the field of biological medicine, in particular to an application of Rakicidins compounds in resistance to clinical pathogenic anaerobic bacteria. Pharmacodynamic experiments indicate that the Rakicidins compounds have good resistant effect on the clinical pathogenic anaerobic bacteria and have the resistant effect on vancomycin enterococcus infection diseases. The Rakicidins compounds can be used for treating diarrhea, enteritis, alimentary infection, oral infection or skin acne caused by clostridium difficile as well as diseases such as urinary tract infection, or skin soft-tissue infection and the like.

The invention relates to the technical field of application of bacteroides fragilis, and in particular relates to application of bacteroides fragilis in preparing a composition for preventing and treating acute radiation enteritis. Experiments show that bacteroides fragilis is safe, nontoxic and strong in pharmacologic action and has a good preventing and treating effect to acute radiation enteritis, thereby indicating that the bacteroides fragilis has good edible and officinal prospects. The invention explores novel use of bacteroides fragilis and develops a novel application field. Bacteroides fragilis serving as probiotic can be used for preparing foods or medical compositions for preventing and treating acute radiation enteritis.

The invention provides lactobacillus plantarum resistant to enteritis salmonella infection and the application thereof and belongs to the technical field of microorganisms.The lactobacillus plantarum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on Sep, 24th, 2015 with the preservation number of CGMCC No.11446.The lactobacillus plantarum CGMCC No.11446 has high acid and cholate resistance, and has a remarkable inhibition effect on growth of enteritis salmonella.Adhesion of enteritis salmonella to enterocyte HT-29 is strongly inhibited, and enteritis salmonella infection is prevented or relieved.The lactobacillus plantarum CGMCC No.11446 can be used for preparing pharmaceutical compositions for preventing or relieving enteritis salmonella, food containing activity lactobacillus and fermented food, and has broad application prospects.

The invention provides Gegenqinlian extractive which is obtained by mixing four medicines of kudzuvine roots, baikal skullcap roots, goldthread roots, and licorice roots in the weight ratio of 8: 3: 3: 2, extracting effective parts and refining. The extractive has the main effective parts in the following weight percentage: total isoflavone of the kudzuvine roots is 13.5 to 21.0 percent, total flavone of the baikal skullcap roots is 20.5 to 32.5 percent, total alkaloid of the goldthread roots is 10.5 to 18.5 percent, and glycerrhizic acid is 2.0 to 5.0 percent. Through adding medicinal auxiliary materials into the extractive, and adopting a conventional method, various peroral preparation for treating bacillary dysentery, acute enteritis, and infantile virus diarrhea can be prepared.

The invention relates to a traditional Chinese medicinal composition for treating gastrointestinal tract, in particular to a medicinal composition for treating gastrointestinal tract diseases such as acute and chronic gastritis, enteritis, gastric ulcer, superficial gastritis, gastric acid, gastrectasia, indigestion and the like. The invention also relates to a preparation of the medicinal composition. The traditional Chinese medicinal composition for treating gastrointestinal tract comprises the medicaments in percentage by weight: 10-25 parts of red ginseng, 10-23 parts of poria, 8-20 parts of bighead atractylodes rhizome, 10-25 parts of rhizoma ligustici wallichii, 10-25 parts of rhizoma atractylodis, 10-25 parts of cyperus rotundus, 10-25 parts of medicated leaven, 10-25 parts of gardenia, 10-23 parts of betelnut, 8-20 parts of radix aucklandiae, 5-15 parts of liquorice, 5-8 parts of malt and 10-25 parts of hawthorn. The medicinal composition for treating the gastrointestinal tract is orally taken and has the efficacies of regulating the flow of qi, comforting the stomach, diminishing inflammation, killing pain, repairing gastrointestinal mucosa and ulceration, enhancing gastrointestinal motility, adjusting gastric acid secretion, promoting digestive absorption and the like.

Methods of treating, reducing, or preventing a disease or symptom relating to a disease such as oral mucositis, gingivitis, periodontitis, gastric mucositis, inflammatory fibrosis, gastritis, colitis, ileitis, Crohn's disease, chronic inflammatory intestinal disease, inflammatory bowel syndrome, chronic inflammatory bowel disease, celiac disease, ulcerative colitis, a gastric ulcer, a peptic ulcer, a buccal ulcer, a nasopharyngeal ulcer, an esophageal ulcer, a duodenal ulcer, a gastrointestinal ulcer, an autoimmune disorder, inflammation associated with cancer, cancer treatment, or radiation, and / or pain that include: identifying a patient in need of treatment; and administering a therapeutically effective amount of at least one cationic steroid antimicrobial (CSA), or a pharmaceutically acceptable salt thereof. Kits comprising such compositions and instructions on such methods are also contemplated herein.

The invention relates to nutrition intestinal flora prevention and / or allergic disease treatment traditional Chinese mineral medicine and inorganic salt compound preparation, which is characterized inthat per 100g of the traditional Chinese mineral medicine and inorganic salt compound preparation is prepared from the following ingredients in proportion by weight (gram): 1 to 98g of traditional Chinese mineral medicine composition, 0 to 98g of oxides, 0 to 10g of sulfate, 0 to 10g of halogenide, 0 to 10g of carbonate, 0 to 10g of phosphate, 0 to 10g of silicate, 0 to 10g of borate, 0 to 10g ofsulfide, 0 to 10g of nitrate and 0 to 10g of molybdate. The body immunity can be improved through improving the diversity of the enteric microorganism and promoting the growth of various probiotics;the body immunity is improved; the allergic disease, inflammatory enteritidis and autism can be further cured.

The invention relates to a method for detecting bacteria, in particular to a method for detecting enteritis pathogenic bacteria. The method comprises the following steps of: (1) extracting DNA (Deoxyribonucleic Acid) of one or more bacteria samples; (2) amplifying full length of 16S rDNA (ribosomal DNA) of a sample by using a primer of 16S rDNA of bacteria; (3) breaking an amplified PCR (Polymerase Chain Reaction) product; (4) repairing ends of the broken product from each bacteria sample and connecting deoxyadenosine at the end 3' of the product, and then connecting different PCR-free joints; (5) sequencing a connection product by using a second-generation sequencing technology to obtain a sequence of the connection product; and (6) comparing the sequence with 16S rDNA reference sequences of all existing bacteria to determine types of bacteria existing in each sample.

The invention relates to a multiplex PCR-based synchronous and rapid method for detecting 13 pathogenic microorganisms in water, which comprises the steps of using multiplex PCR to simultaneously amplify gene-specific fragments of the 13 pathogenic microorganisms including escherichia coli, enterohaemorragic escherichia coli o157:h7, legionella pneumophila, salmonella enteritidis, shigella dysenteriae, staphyloccocus aureus, listeria monoeytogenes, helicobacter pylori, mycobacterium tuberculosis, klebsiella pneumonia, vibrio cholera, bacillus anthracis and yersinia pestis, and detecting PCR amplified products through agarose gel electrophoresis, thereby achieving synchronous and rapid detection for the 13 pathogenic microorganisms.

The invention discloses saccharomyces cerevisiae 5-1 which is collected in the Guangdong Microbial Culture Collection Center, with the collection number of GDMCC No: 60243. The invention further discloses a fermentation solution, a culture and a culture dilution prepared from the saccharomyces cerevisiae 5-1. A metabolite of the saccharomyces cerevisiae 5-1 has a very strong inhibitory effect on pathogenic bacteria (escherichia coli, staphylococcus aureus and salmonella enteritidis); when the saccharomyces cerevisiae 5-1 is used as a feed additive and is added into feed for feeding animals, the disease incidence of the animals can be effectively reduced, the growth performance of the animals can be improved, and the intestinal microflora of the animals can be significantly improved, so that the saccharomyces cerevisiae 5-1 is of a great significance in development of the breeding industry.

The invention discloses a salmonella enteritidis double knockout attenuated mutant and preparation as well as application thereof. The salmonella enteritidis double knockout attenuated mutant is obtained by knocking out crp gene and spiC gene of salmonella enteritidis C50041. The invention further discloses a preparation method as well as application of the salmonella enteritidis double knockout attenuated mutant. Further attenuation of the salmonella enteritidis attenuated mutant is realized. A foundation is laid for researching salmonella enteritidis attenuated live vaccines and live vector vaccines.

The invention discloses an artificial feed for aquarium fishes. The artificial feed comprises the components of fish meal, antibacterial peptide, raw bran, soybean meal, calcium dihydrogen phosphate, cuttlefish cream, fish oil, phosphatide oil, premix, earthworm powder, fly larva powder, yeast powder, shrimp powder and bacillus. The artificial feed disclosed by the invention can increase the breeding survival rate of the aquarium fishes, can accelerate the growth of the aquarium fishes, can reduce the enteritis attack rate of carnivorous aquarium fishes in a breeding process, and can contribute to realizing large-scale popularization of the breeding of the aquarium fishes.

The invention discloses a Salmonella enteritidis nucleic acid standard sample as well as a building method and application thereof. The building method comprises the steps: strain culture, identification, preparation of a nucleic acid standard sample, drying of the nucleic acid standard sample, uniformity and stability inspection, qualitative assay, rating of the nucleic acid standard sample and other operations to obtain the Salmonella enteritidis nucleic acid standard sample. According to the invention, the condition that the pathogen nucleic acid standard sample is deficient in domestic food is solved, has important practical significance in realizing preparation technology of the pathogen nucleic acid standard sample in food and stability guarantee technology, positively developing the research and development of pathogen molecular biological detection standard sample in domestic food, and filling up the blank of the measuring field, and has a broad market in developing molecular biological detection working of pathogen in the food and food pathogen nucleic acid standard sample, and has greater economic benefits and social benefits.

The invention discloses a multifunctional probiotic, and belongs to the technical field of the preparation of probiotic preparation. The probiotic contains the following components active substances: Lactobacillus casei, Bacillus licheniformis, Bacillus subtilis, Bacillus cereus, Saccharomyces cerevisiae and Pseudomonas stutzeri. The multifunctional probiotic composition provided by the invention can be prepared into a solution for preparation of beneficial bacteria forage, can be used for deodorization of animal dung, prevention and treatment of enteritis and diarrhea of animals, and has wide range of application.

The invention provides a composite microecological feed additive capable of increasing the freshwater fish growth rate and application of the composite microecological feed additive. The additive comprises the following components: aspergillus niger solid fermentation compound enzyme, bacillus subtilis, bacillus cereus, cellulose, xylanase, neutral protease and phytase. The composite microecological feed additive disclosed by the invention is added with the solid fermentation compound enzyme, so that the utilization rate of the freshwater fish to baits is effectively increased, the growth of fishes is promoted, the breeding of pathogenic bacteria including aeromonas hydrophila, vibrio alginolyticus and the like in a water body can be inhibited due to the addition of fungicides, the occurrence possibility of diseases of saprolegniasis, white skin diseases, bacillary enteritis and the like is reduced; compared with similar additives, the additive disclosed by the invention has the advantages of being safe, high in efficiency, special, free of residues, free of side effects and the like; the composite microecological feed additive can be used for improving and keeping the good growth state of the freshwater fish and effectively increasing the freshwater fish growth rate.

The present invention discloses a grass carp enteritis treatment drug composition, which contains, by weight, 10-30 parts of humulus scandens, 20-30 parts of humifuse euphorbia herb, 2-10 parts of melia azedarach leaf, 20-25 parts of heartleaf houttuynia herb, 2-10 parts of arborvitae leaf, and 2-7 parts of garlic. The invention further discloses a preparation method for the drug composition. According to the grass carp enteritis treatment drug composition, honeysuckle provides an anti-pathogenic microorganism effect, a certain inhibition effect is provided for a variety of pathogenic bacteria such as staphylococcus aureus, hemolytic streptococcus, escherichia coli, shigella dysenteriae, vibrio cholerae, typhoid bacillus, paratyphoid bacillus and the like, and a certain inhibition effect is further provided for pneumococcus, meningococcus, pseudomonas aeruginosa, and tubercle bacillus. The preparation method is simple and easy to perform, and is suitable for mass production.

The invention provides a salmonella broad-spectrum virulent bacteriophage. The preservation number of the bacteriophage is CCTCC NO: M 2020839. The invention also provides a preparation process and application of the salmonella broad-spectrum virulent bacteriophage, and the preparation process comprises fermentation and extraction; in the step of fermentation, a salmonella gallinarum attenuated vaccine strain is used as host bacteria for bacteriophage fermentation. The bacteriophage XPARCPS02 disclosed by the invention can be used for cracking salmonella of various serotypes and also has different degrees of cracking effects on drug-resistant bacteria; the salmonella typhimurium lysate lysate has a cracking capability on salmonella typhimurium, salmonella typhimurium drug-resistant bacteria, salmonella gallinarum, salmonella enteritidis, salmonella enteritidis drug-resistant bacteria, salmonella typhimurium, salmonella pullorum, salmonella paratyphi A, salmonella paratyphi B and salmonella dubicularis.

The invention discloses a salmonella phage RDP-NSA-19050 with a wide lysis spectrum, wherein the preservation number is CGMCC No.21415, the gene length is 87591 bp; after sequencing is carried out on whole genes, it is found that the salmonella phage RDP-NSA-19050 does not have virulence genes and lyogenic genes, and the salmonella phage RDP-NSA-19050 has a good lysis spectrum. The salmonella bacteriophage RDP-NSA-19050 provided by the invention can be used for cracking a plurality of pathogenic bacteria such as salmonella steulani, salmonella gallinarum, salmonella typhimurium, salmonella paratyphi A, salmonella paratyphi B, salmonella typhimurium, salmonella enteritidis and salmonella kkinensis; the salmonella bacteriophage RDP-NSA-19050 has the characteristics of wide splitting spectrum and high splitting rate, can be independently used, can also be compounded with bacteriophages of other escherichia coli and salmonella and probiotics for use, and can effectively reduce the death rate of chicken infected with salmonella.

Gut health in poultry is improved by providing an efficacious amount of extracts of tannic acid via feed or via drinking water. The extracts of tannic acid are also effective when the poultry are infected with coccidiosis and / or necrotic enteritis. In alternative embodiments, an efficacious amount of Bacillus coagulans is also included.

Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum, and Salmonella pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage of the present invention can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum or Salmonella pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the Salmonella bacteria. The present invention also provides important insights into prevention and control strategies against Salmonella infection and suggests that the use of bacteriophage can be a novel, safe, and effectively plausible alternative to antibiotics for the prevention of Salmonella infection in poultry.

Disclosed herein are is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria. Disclosed are also compositions, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The invention relates to bacillus subtilis having effects of relieving enteritis and promoting development of intestines. The bacillus subtilis is named as RZ001, the category name is Bacillus subtilis., the preservation No. is CGMCC NO:17116, the preservation date is 7, January, 2019, the preservation address is No.3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, and the preservation unit is China General Microbiological Culture Collection Center(CGMCC). The bacterial strain has the functions of repairing and strengthening mucosal barrier, has the function of treating inflammatory and allergic dermatosis enteritis, and can also promote the development of the intestines. The bacterial strain is hopeful to be used for preparing drugs and fermented foods for preventing andtreating intestine inflammation and promoting development, and has broad application prospect.

The invention discloses a medicine for treating gastrointestinal diseases by utilizing biological live bacteria, which is prepared from the following substances: 1 to 6 weight portions of oligoisomaltose, 1 to 6 weight portions of glucose, as well as 1 to 10 hundred million CFU / gram of bifidobacteria, 1 to 10 hundred million CFU / gram of lactobacilli and 1 to 10 hundred million CFU / gram of probiotics which take the total weight of the oligoisomaltose and the glucose as a radix number, wherein the probiotics are at least one of bacillus, lactococcus and enterococcus. The medicine is based on a modern microecology theory, adopts a probiotic live bacteria preparation to treat diseases such as gastritis, gastric ulcer, enteritis, diarrhea, constipation, indigestion, abdominal distension, diarrhea, intestinal cancer, acute and chronic hepatitis, low immunity and the like, and has the advantages of reliable action, broad function, safe use, no any toxic side effect and irritation, convenient administration and carrying and the like.