41 results about "Fowl typhoid" patented technology

Disclosed herein are is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria. Disclosed are also compositions, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum. Further, the present invention relates to a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella Enteritidis or Salmonella Typhimurium, Fowl Typhoid caused by Salmonella Gallinarum, and Pullorum disease caused by Salmonella Pullorum, comprising the bacteriophage as an active ingredient. Furthermore, the present invention relates to a feed additive, drinking water, a cleaner and a sanitizer, comprising the bacteriophage as an active ingredient.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella enteritidis or Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient.

Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum, and Salmonella pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage of the present invention can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum or Salmonella pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the Salmonella bacteria. The present invention also provides important insights into prevention and control strategies against Salmonella infection and suggests that the use of bacteriophage can be a novel, safe, and effectively plausible alternative to antibiotics for the prevention of Salmonella infection in poultry.

The invention discloses an inert carrier indirect agglutination test detection system and application thereof. The system comprises inert carrier bacteria S9 and a complex S9-P which is shown and expressed on the surface of the inert carrier bacteria S9 and carries a P-antibody factor. The system only carries the P-antibody factor, is single in component and specific in targeting, generates macroscopic positive particle agglutination reaction with whole blood or serum of chicken infected by pullorum disease and salmonella typhimurium under a certain concentration condition, and does not generate non-specific cross agglutination reaction with chicken-derived serum and whole blood of different backgrounds infected by non-pullorum disease and salmonella typhimurium. The S9-P is based on a glass plate agglutination reaction operation platform. The S9-P is simple and convenient to operate, sensitive and rapid in reaction, macroscopic in agglutination reaction particles and clear and easy inresult judgment, tests and result judgment are completed within two minutes, and the S9-P is suitable for targeted specific detection of pullorum disease and salmonella gallinarum infection in chicken flocks and has a good application prospect in on-site monitoring and diagnosis of the chicken flocks.

The invention discloses primers, probes, a test kit and a test method for triple real-time fluorescence PCR detection of Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum. According to the present invention, a triple real-time PCR method is used, wherein a consensus sequence of Salmonella choleraesuis and Salmonella paratyphi is used for the detection of Salmonella choleraesuis and Salmonella paratyphi, a specific sequence of Salmonella typhi is used for the detection of Salmonella typhi, and a specific sequence of Salmonella gallinarum is used for the detection of Salmonella gallinarum. Whether a sample is contaminated by Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum is determined via a real-time fluorescence PCR amplification, and then Salmonella choleraesuis and Salmonella paratyphi are distinguished via a single fluorescent PCR amplification; the detection is rapid, the process of preparing sample and issuing test results can be finished in 31hours, the results are reliable, and sensitivity and specificity are high.

The invention belongs to the field of biotechnology detection, and particularly relates to a PCR detection kit for rapidly identifying Salmonella Pullorum/Salmonella Gullinarum, wherein the PCR detection kit comprises a SPUL_2693 gene detection primer pair, and the SPUL_2693 gene detection primer pair comprises a forward primer with a nucleotide sequence represented by SEQ ID NO.1 and a reverse primer with a nucleotide sequence represented by SEQ ID NO.2. According to the present invention, the kit can perform rapid and high-throughput identification on Salmonella Pullorum/Salmonella Gullinarum, can be used as the auxiliary method for the traditional serological typing of Salmonella, and provides the new simple, rapid and highly-sensitive method for the monitoring and laboratory diagnosisof Salmonella Pullorum/Salmonella Gullinarum.

The invention relates to a traditional Chinese medicine composition for treating fowl typhoid and a preparation method thereof. The traditional Chinese medicine composition comprises the following ingredients, by weight, 20-40 parts of the root of Chinese pulsatilla, 10-20 parts of phellodendron, 10-20 parts of Scutellaria baicalensis, 10-20 parts of radix platycodi, 5-15 parts of honeysuckle flower, 10-20 parts of humifuse euphorbia herb and 10-20 parts of licorice. The traditional Chinese medicine composition has antimicrobial, anti-inflammation, heat-clearing and antidiarrheal effects, has little toxic and side effect, has no drug residue, is not easy to generate drug resistance, is convenient to use, is easy to absorb, has high bioavailability, meets requirements of safe veterinary drug and animal-derived food safety guarantee, and is an ideal traditional Chinese medicine composition for treating fowl typhoid.

The invention relates to a veterinary compound gentamycin sulfate injection and a preparation method thereof. The injection consists of gentamicin sulphate, ofloxacin, analgin, trimethoprim, an organic solvent and injection water. The injection serving as a veterinary special compound preparation is mainly used for treating porcine epidemic diarrhea, transmissible gastrocnteritis, paratyphus vaccine for piglets, yellow and white scour for piglets, edema disease for piglets, and intestinal diseases, such as poultry colibacillosis, fowl typhoid, paratyphoid and the like. The injection has the advantages of convenient use, short period of treatment, low medicament resistance and the like.

The invention belongs to the field of fowl salmonellosis prevention and control and particularly relates to a pullorum disease salmonella spiC-rfaH double-gene knockout attenuated strain and preparation and application thereof. The attenuated strain is pullorum disease salmonella in which a spiC gene or an rfaH gene is not expressed. It is found through animal experiments that virulence of the mutant strain is remarkably reduced, and colonization time in a host body is shortened obviously. The attenuated strain is adopted for preparing a pullorum disease salmonella live vaccine, after chickens are inoculated with the vaccine, no adverse reaction is generated, and strong virulence strain infection can be effectively eliminated. The host does not generate an anti-body aiming at O-antigen, by means of the pullorum disease fowl typhoid multi-valence detection antigen, the attenuated stain can be effectively applied to glass plate agglutination experiments to distinguish immunized chickens from naturally infected chickens, and the characteristic of the DIVA vaccine is achieved.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella typhimurium, Salmonella gallinarum, or Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient. The present invention also provides important insights into prevention and control strategies against Salmonella infection and suggests that the use of bacteriophage can be a novel, safe, and effectively plausible alternative to antibiotics for the prevention of Salmonella infection in poultry.

The invention provides a water-soluble trimethoprim composition and a compounded composition thereof. After being treated through a special process, trimethoprim can be uniformly dispersed in water together with sulfanilamide salt, and can be used for effectively solving the problems that a premixing agent is unevenly mixed in the feed and influences the treatment effect. The water-soluble trimethoprim composition is applied through hydroposia, has obvious treatment effects on blood poisoning, white diarrhea, fowl typhoid, cholera, unknown high heat, respiratory system secondary bacterial infection and the like caused by poultry colibacillus, and can be used for preventing and treating coccidiosis. The preparation method is simple in operation, free from pollution and suitable for industrial production.

The invention provides a multiple PCR primer group for avian escherichia coli, chicken typhoid salmonella, salmonella pullorum, salmonella enteritidis and bacillus breslaviensis, a quintuple PCR detection kit comprising the primer group and a quintuple PCR detection method. The primer group comprises primer pairs ECphoA-F and ECphoA-R for specific amplification of avian escherichia coli phoA genes, primer pairs SGg1gC-F and SGg1gC-R for specific amplification of chicken typhoid salmonella glgC genes, primer pairs SGPspeC-F and SGPspeC-R for specific amplification of chicken typhoid salmonella and salmonella pullorum genes, primer pairs SEsdf I-F and SEsdf I-R for specific amplification of salmonella enteritidis sdf-1 genes and primer pairs STstm4495-F and STstm4495-R for specific amplification of bacillus breslaviensis stm4495 genes.

The invention belongs to the field of fowl salmonellosis prevention and control and particularly relates to a pullorum disease salmonella spiC-rfaL double-gene knockout attenuated strain and preparation and application thereof. The attenuated strain is pullorum disease salmonella in which a spiC gene or an rfaL gene is not expressed. It is found through animal experiments that virulence of the mutant strain is remarkably reduced, and colonization time in a host body is shortened obviously. The attenuated strain is adopted for preparing pullorum disease salmonella live vaccine, after chickens are inoculated with the vaccine, no adverse reaction is generated, and strong virulence strain infection can be effectively eliminated. The host does not generate an anti-body aiming at O-antigen, by means of pullorum disease fowl typhoid multi-valence detection antigen, the attenuated stain can be effectively applied to glass plate agglutination experiments to distinguish immunized chickens and naturally infected chickens, and the characteristic of the DIVA vaccine is achieved.

The invention discloses a salmonella bacteriophage CKT1 without transduction of drug-resistant gene and application of the salmonella bacteriophage CKT1, the virulent bacteriophage CKT1 is preserved in China Center for Type Culture Collection on January 21, 2022, and the preservation number of the virulent bacteriophage CKT1 is CCTCC (China Center for Type Culture Collection) 2022110. The separated salmonella bacteriophage CKT1 has splitting activity on D-group salmonella such as salmonella pullorum, salmonella gallinarum and salmonella enteritidis from different sources, is short in incubation period, high in progeny bacteriophage outbreak amount and low in bacteriophage mutation resistance rate, does not carry virulence genes and drug-resistant genes on a genome, does not have transduction capability, and can be used for preparing the salmonella bacteriophage CKT1. The bacteriophage does not cause transmission of drug-resistant genes, is a safe, efficient and high-specificity virulent bacteriophage, and does not destroy intestinal flora of chicks. The bacteriophage preparation can become a novel environment-friendly auxiliary means for purifying salmonella pullorum in a breeding chicken farm, and is also an alternative strategy for preventing and treating salmonella pullorum or salmonella gallinarum infection of chicken flocks.

We constructed S. Gallinarum strains deleted for the global regulatory gene fur (FIG. 1) and evaluated their virulence and protective efficacy in Rhode Island Red chicks and Brown Leghorn layers. The fur deletion mutant was a virulent and, when delivered orally to chicks, elicited excellent protection against lethal S. Gallinarum challenge. We also examined the effect of a pmi mutant and a combination of fur deletions with mutations in the pmi and rfaH genes, which affect O-antigen synthesis, and ansB, whose product inhibits host T cell responses. The ΔAfur Δpmi and Δfur ΔansB double mutants were attenuated, but not protective when delivered orally to chicks. However, a Δpmi Δfur strain was substantially immunogenic when administrated intramuscularly. Altogether our results show that the fur gene is essential for virulence of S. Gallinarum and the fur mutant is effective as a live recombinant vaccine against fowl typhoid.