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Novel bacteriophage and antibacterial composition comprising same

A technology of phage and composition, applied in the field of new phage, to achieve the effect of excellent acid resistance

Inactive Publication Date: 2011-08-10
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, misuse and overuse of antibiotics has led to more concerns about antibiotic resistance and harmful effects of antibiotic residues in food (Cislo, Metal. Bacteriophage treatment of suppurative skin infections. Arch Immunol. Ther. Exp. 1987.2: 175-183 ; Kim sung-hun et al., Bacteriophage; New Alternative Antibiotics. Biological research information center (BRIC))

Method used

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  • Novel bacteriophage and antibacterial composition comprising same
  • Novel bacteriophage and antibacterial composition comprising same
  • Novel bacteriophage and antibacterial composition comprising same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1: Salmonella phage isolation

[0083] 1-1. Phage screening and single phage isolation

[0084] Transfer 50 ml of samples from chicken slaughterhouse and sewage water into a centrifuge tube and centrifuge at 4000 rpm for 10 minutes. The supernatant was then filtered using a 0.45 μm filter. Mix 18ml sample filtrate with 150μl ST shaking medium (OD 600 = 2) Mix with 2ml of 10×Luria-Bertani medium (hereinafter referred to as LB medium, tryptone 10g; yeast extract 5g; NaCl 10g; final volume is 1L). The mixture was incubated at 37°C for 18 hours, and the medium was centrifuged at 4000 rpm for 10 minutes. Filter the supernatant with a 0.2μm filter. Mix 3ml 0.7% agar (w / v) and 150μl ST shaking medium (OD 600 = 2) Mix and plate on LB plate to make it a solid medium. Coat 10μl of culture filtrate on it and incubate at 37°C for 18 hours (0.7% agar is used as "top-agar" and the titration of phage lysate is performed on top agar, called soft agar Covering method (softagar o...

Embodiment 2

[0088] Example 2: Investigation of Salmonella infection with ΦCJ3

[0089] In order to examine the lytic activity of the selected phage against other Salmonella and ST, try to cross-infect with other Salmonella. As a result, ΦCJ3 did not infect SC (Salmonella enterica Serotype Choleraesuis), SD (Salmonellla enterica Serotype Derby), SA (Salmonella enterica subsp. Arizonae)), SB (Salmonella enterica subsp. Bongori), but specifically infected SG, SP, ST and SE (see Example 12). The results are shown in Table 1 below. The phage ΦCJ3 produced using SG as the host cell showed the same plaque size and plaque turbidity as well as the same protein pattern and genome size as those produced using ST as the host cell.

[0090] [Table 1]

[0091]

[0092] Serotype

Strain name

Plaque formation

Serotype

Strain name

Plaque formation

SE

SGSC 2282

O

SA

ATCC

13314

X

ST

ATCC 14028

O

SB

ATCC

43975

X

SG

SGSC 2293

O

SC

ATCC

10...

Embodiment 3

[0095] Example 3: Morphological investigation of bacteriophage ΦCJ3

[0096] The purified ΦCJ3 was diluted in 0.01% gel solution, and then fixed in 2.5% glutaraldehyde solution. After the sample was dropped on a carbon-coated mica plate (approximately 2.5×2.5 mm) and adapted for 10 minutes, it was washed with sterile distilled water. The carbon film was placed on a copper mesh and dyed with 4% uranyl acetate for 30-60 seconds, dried, and examined under a JEM-1011 transmission electron microscope (80kV, magnification×120,000~×200,000). As a result, the purified ΦCJ3 has morphological features including an isometric capsid and a long retractable tail, such as figure 1 > As shown in, this indicates that it belongs to the morphotype group of the Mycophagaceae family.

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Abstract

The present invention relates to a novel bacteriophage, and more specifically to a bacteriophage that can specifically destroy one or more Salmonella spp. selected from a group consisting of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum. The present invention further relates to a composition containing the bacteriophage as an active ingredient for the prevention or treatment of infectious diseases such as salmonellosis and salmonella foodborne intoxication induced by Salmonella enteritidis or Salmonella typhimurium, fowl typhoid induced by Salmonella gallinarum, pullorum induced by Salmonella pullorum, etc. Moreover, the present invention relates to animal feeds, drinking water, detergent and disinfectant containing the bacteriophage as an inactive ingredient.

Description

Technical field [0001] The present invention relates to a novel bacteriophage, and more specifically to a bacteriophage having specific bactericidal activity against one or more Salmonella selected from the group consisting of: Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum and (Salmonella Gallinarum) Salmonella Pullorum (Salmonella Pullorum). Further, the present invention relates to a composition for preventing or treating infectious diseases, the infectious diseases including salmonellosis caused by Salmonella enteritidis or Salmonella typhimurium and Salmonella food poisoning, typhoid fever caused by Salmonella gallisepticum and Salmonella pullorum Of chicken pullorum, the composition contains the phage as an active ingredient. Moreover, the present invention relates to feed additives, drinking water, detergents and sanitary disinfectants, which contain the bacteriophage as an active ingredient. Background technique [0002] Salmonella is a genus of E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/00C12N7/01
CPCC12N2795/10121C12N7/00A61K35/13A61P31/04Y02A50/30C07K7/00
Inventor 姜寅惠朴敏兌赵英郁申秀安崔香
Owner CJ CHEILJEDANG CORP
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