47 results about "Salmonella enterica serovar enteritidis" patented technology

The invention discloses application of pyroptosis-associated protein GSDMD (Gasdermin-D) for preparing a bacterial ghost vaccine. A bacteriolysis plasmid containing a pyroptosis-associated protein GSDMD coding gene is constructed and is converted into salmonella enteritidis; under the induction of gum sugar, bacteria are split to successfully obtain a salmonella novel bacterial ghost vaccine. An experiment proves that GSDMD mediated bacteriolysis has an extremely long persistent period, and a splitting rate is 99.9985% or more. After a novel bacterial ghost immune mouse prepared by the invention is adopted, an organism can be stimulated to generate powerful humoral immunity and cellular immunity; protective immunity response is induced; an experiment animal can be protected so as to resistsalmonella infection. The salmonella bacterial ghost vaccine prepared by the invention has a good immune protection effect.

Described herein is the identification of a novel Salmonella enterica serovar Enteritidis locus that serves as a marker for DNA-based identification of this bacterium. In addition, three primer pairs derived from this locus that may be used in a nucleotide detection method to detect the presence of the bacterium are also disclosed herein.

The invention discloses a salmonella bacteriophage with a wide lysis spectrum and high temperature resistance. The preservation number is CGMCC No.21995. After whole genes are sequenced, the salmonella bacteriophage does not have virulence genes and lyogenic genes. The salmonella bacteriophage RDP-SA-20049 acts for 60 min at the high temperature of 90 DEG C, the titer is kept at 1.0 * 10 < 7 > pfu/mL or above, and the salmonella bacteriophage RDP-SA-20049 has high biological activity and good high temperature resistance. The salmonella bacteriophage can be used for lysing pathogenic bacteria including 5 strains of salmonella typhimurium, 4 strains of salmonella pullorum, 1 strain of salmonella paratyphi B, 1 strain of salmonella typhimurium, 7 strains of salmonella enteritidis and salmonella BS-20036, and has the characteristic of high cracking rate, the cracking rate is up to 99%, the cracking performance is stronger, and the salmonella bacteriophage has good advantages in treatment of salmonella infection diseases, and is high in titer and good in prevention and treatment effect.

The invention relates to salmonella broad-spectrum lyase with in-vitro lytic activity and application thereof, and belongs to the technical field of biology. The lyase is named as LysMD19, an amino acid sequence of the lyase is shown as SEQ ID No. 1, and a nucleotide sequence of the lyase is shown as SEQ ID No. 2. The lyase can directly and efficiently lyse salmonella in-vitro under the conditionof no cell permeation reagents such as ETDA, the lysing effect is achieved on salmonella enteritidis, salmonella typhimurium, salmonella pullorum and other salmonella of different serotypes, good temperature stability and high acid and alkali resistance are achieved, the lyase can be used for preventing and treating salmonella infection, and also can be used for killing salmonella in multiple links such as source farms, processing, packaging and transportation to market selling, the safety 'from farms to dining tables' is ensured, and good application prospects and values are achieved.

The invention discloses a chicken ring RNA Chr26:2670958/2679178 detection primer, a method and application. The primer is prepared from a chicken ring RNA Chr26: 2670958/2679178 annular upstream primer body and a chicken ring RNA Chr26: 2670958/2679178 annular downstream primer body, wherein nucleotide sequences of the chicken ring RNA Chr26: 2670958/2679178 annular upstream primer body and the chicken ring RNA Chr26: 2670958/2679178 annular downstream primer body are respectively shown in SEQ ID NO.1 and SEQ ID NO.2. By means of the method disclosed by the invention, the relative expressionsituation of the chicken ring RNA Chr26: 2670958/2679178 can be accurately detected after a chicken is infected by salmonella enteritidis; the relative expression situation is utilized as a molecularmarker to identify salmonella infection resistance individuals; thus, theoretical basis and scientific basis are provided for salmonella enteritidis infection resisting genetic breeding of chickens.

The invention discloses salmonella phage SEE-1 and an application thereof, relates to prevention and control of salmonella, and belongs to the field of bioengineering. A novel bacteriophage SEE-1 is separated from a sewage treatment system, the preservation No. is CGMCC NO.1834D, the basic property of genomes is analyzed, and particularly, functional genes and protein are predicted and analyzed. The separated salmonella phage SEE-1 shows quick and efficient splitting efficiency in experiment of splitting the bacterium enteritidis, and can be used for preventing acterium enteritidis or drug resistant acterium enteritidis in the sewage treatment system.

The present invention relates to a new cocktail of bacteriophages with specific lytic activity against Salmonella enteritidis, Salmonella typhimurium and Salmonella paratyphi B., for reducing, eliminating and/or preventing them in farm animals and animals from the poultry sector, such as poultry, hens and breeding hens, in addition to eggs. It may be administered as an additive in the feed, in water or by spray. Moreover, the cocktail may be used as a disinfectant in work areas of farms and abattoirs, and in processed foods, without affecting the organoleptic properties of the product.

The invention discloses a dual fluorescence PCR primer group, a kit and a method for detecting salmonella enteritidis and mycoplasma synoviae, the primer group provided by the invention comprises specific primers aiming at salmonella enteritidis and mycoplasma synoviae, the length of each primer is about 20 basic groups, the GC content is 40-60%, no complementary sequence exists among the primers, and the specific primers can be used for detecting the salmonella enteritidis and mycoplasma synoviae in the salmonella enteritidis and mycoplasma synoviae in the salmonella enteritidis and mycoplasma synoviae in the salmonella enteritidis and mycoplasma synoviae. The melting temperature difference is less than 5 DEG C, and no complementary sequence or secondary structure exists in the primer. The kit provided by the invention simultaneously contains the primer groups of the salmonella enteritidis and the mycoplasma synoviae, the four primers do not interfere with one another during amplification, two pathogens can be screened by one-time amplification reaction by using the kit provided by the invention, the defect that an existing kit cannot simultaneously detect the salmonella enteritidis and the mycoplasma synoviae is effectively overcome, and the kit is suitable for large-scale popularization and application of the salmonella enteritidis and the mycoplasma synoviae. Powerful technical support is provided for purification of chicken enteritis salmonellosis and mycoplasma synoviae in chicken farms, and the method has high practical value and wide application prospect.

The invention relates to the technical field of microorganisms, in particular to a poultry salmonella vaccine, and further relates to application of salmonella. The chicken salmonella enteritidis inactivated vaccine composition comprises chicken salmonella enteritidis liquid and an adjuvant, and the adjuvant is prepared from, by weight, 50 parts of a water-based oil adjuvant, 1.5-3.5 parts of Span-80, 1.5-2.5 parts of Tween-80, 0.1 part of small peptide and 1.5-2.5 parts of propolis. The enteritidis inactivated vaccine is high in pertinency, the immune effect on locally epidemic salmonella strains, the preparation process is easy to operate, the cost is low, storage is easy, and the chicken salmonella enteritidis inactivated vaccine is suitable for large-scale farms with laboratories.

The invention belongs to the technical field of salmonella enteritidis detection, and discloses a quantum dot fluorescence immunochromatography test strip for detecting salmonella enteritidis and a preparation method thereof. Assembling a quantum dot fluorescence immunochromatography test strip, coating a salmonella capture antibody Ab1 and goat anti-mouse IgG on an NC membrane by using a contact type automatic membrane scratching instrument to serve as a detection line T and a quality control line C, and drying; and assembling the sample pad, the combination pad, the NC membrane coated with the antibody and absorbent paper on a PVC (polyvinyl chloride) bottom plate, cutting into a test strip by using a numerical control strip cutting machine, and storing in a dark place under a dry condition for later use. The quantum dot fluorescence immunoassay test strip has the advantage of quantitative detection, and compared with a colloidal gold immunochromatography test strip which is mostly applied in the current market, the quantum dot fluorescence immunoassay test strip uses quantum dots as a marker, so that a fluorescence analyzer can be used for detecting a result, and the salmonella enteritidis can be quantitatively detected by detecting the fluorescence intensity of a detection line.

A method of creating a biotechnological product and an efficient and stable bio-luminescence vector which could be used for tracking Gram-negative bacteria when distributing inside animal body are provided. Through conjugation, this auto-luminescence vector can be easily transmitted from bacteria to bacteria among Gram-negative bacteria, and may facilitate bacteria to be luminescence-labeled for subsequently analyzing the dynamic change of bio-luminescent bacteria within animal body in vivo. This system includes a lacZ promoter-driven luxABCDE, a high copy number of ColE1 replicon, and a high plasmid stability of the conjugative and broad host-ranged plasmid pSE34 from Salmonella enterica serovar Enteritidis Sal550. This resulting construct pSE-Lux1 can not only conjugatively transmit among bacteria with broad host range, but also stably maintain in bacteria to efficiently express the bio-luminescent luxABCDE without supplementing the subtract for luciferases and the antibiotics for plasmid selection.

The invention belongs to the technical field of immunodetection, and discloses a preparation method of a salmonella enteritidis colloidal gold immunochromatography test strip, which comprises the following steps: preparing colloidal gold by using an optimized Tris-base auxiliary method, and coupling the colloidal gold with a salmonella enteritidis monoclonal antibody to obtain an immune label; and assembling the screened sample pad, the combination pad fixed with the immune label, the NC membrane fixed with the T/C line antibody, absorbent paper and PVC (polyvinyl chloride) to obtain the salmonella enteritidis colloidal gold immunochromatography test strip. Colloidal gold with better uniformity and stability is prepared on the basis of a Tris auxiliary method, the salmonella enteritidis colloidal gold immunochromatographic test strip established by taking the colloidal gold as a marker is high in sensitivity, good in specificity and good in repeatability, all properties meet the requirements of clinical rapid detection, and the salmonella enteritidis colloidal gold immunochromatographic test strip has good application prospects. It is proved that the salmonella enteritidis colloidal gold immunochromatography test strip prepared based on the Tris-assisted method can be used for clinical field detection.