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25results about How to "Efficient cracking" patented technology

Ammonia fuel cracking supply system of gas turbine

The invention provides an ammonia fuel cracking supply system of a gas turbine. The system comprises a liquid ammonia storage tank, a liquid ammonia supply pump, an air vaporizer, a heat exchanger, a first ammonia cracker, a compression and expansion all-in-one machine, the gas turbine and the like. Wherein liquid ammonia is supplied to the air vaporizer through the liquid ammonia supply pump; one end of the heat exchanger is communicated with the air vaporizer, and the other end is communicated with the first ammonia cracker; one end of a compression-expansion all-in-one machine introduced by the system is communicated with the heat exchanger, and the other end is communicated with the gas turbine. The air vaporizer adopts an exhaust heat source to gasify liquid ammonia into ammonia gas. Ammonia gas discharged from the air vaporizer enters the heat exchanger, exchanges heat with cracked gas after cracking to be heated, and then is guided into the first ammonia cracker. Ammonia gas is decomposed into mixed gas of hydrogen and nitrogen in the first ammonia cracker, the mixed gas can enter the heat exchanger, and the mixed gas enters the compression and expansion all-in-one machine after being subjected to heat exchange and cooling in the heat exchanger, and is conveyed to the gas turbine after being pressurized and cooled.
Owner:CHINA UNITED GAS TURBINE TECH CO LTD

Ammonia catalytic cracking hydrogen production device based on engine exhaust waste heat utilization, control system and control method

The invention belongs to the field of ammonia cracking hydrogen production devices, and particularly relates to an ammonia catalytic cracking hydrogen production device based on engine exhaust waste heat utilization, a control system and a control method.The ammonia catalytic cracking hydrogen production device comprises an ammonia catalytic cracking hydrogen production system, a nitrogen-hydrogen separation system and a working condition control system; the ammonia catalytic cracking hydrogen production system comprises an exhaust pipe, and an ammonia gas cracking pipe and a heat insulation pipe which sequentially wrap the outer wall of the exhaust pipe; the ammonia gas cracking pipe comprises an ammonia gas inlet and a cracking mixed gas outlet, and a gap is reserved between the inner wall of the ammonia gas cracking pipe and the outer wall of the exhaust pipe and used for circulation and cracking of ammonia gas; the nitrogen-hydrogen separation system comprises a hydrogen separation unit, a nitrogen separation unit and an ammonia gas recovery unit; the cracking mixed gas outlet is connected with the hydrogen separation unit; the ammonia gas recovery unit comprises an ammonia water heating bin, and the exhaust pipe penetrates through the ammonia water heating bin. Ammonia gas cracking and nitrogen-hydrogen separation are completed by fully utilizing engine tail gas waste heat, the cost and energy consumption are remarkably reduced, and hydrogen supply for promoting efficient and reliable combustion of the ammonia engine can be obtained.
Owner:HUAZHONG UNIV OF SCI & TECH

Electric submersible screw pump anti-airlock method and equipment based on working condition recognition and medium

The invention discloses an anti-airlock method and equipment for an electric submersible screw pump based on working condition recognition and a medium, and relates to the technical field of oil exploitation equipment. The method comprises the steps that based on an underground detection device and a ground control cabinet sensor of the electric submersible screw pump unit, operation parameters of the electric submersible screw pump unit are collected in real time, and the operation parameters are preprocessed to obtain standard operation parameters; wherein the operation parameters comprise pump inlet pressure and motor output current; extracting pressure-current correlation characteristics under the airlock working condition to construct an airlock working condition identification model, and inputting the standard operation parameters into the airlock working condition identification model to obtain an airlock state grade; and based on the airlock state grade, the operation regulation and control strategy of the electric submersible screw pump unit is adjusted so as to eliminate the airlock phenomenon. Real-time data of underground and ground sensors are collected, the airlock grade is judged in advance through the airlock working condition recognition model, the operation state is automatically adjusted, and early warning of the airlock is achieved.
Owner:DESHI (XIAN) OIL & GAS LIFTING TECHNOLOGY CO LTD +1

Hair drug detection reagent and detection device

PendingCN119667131AEfficient crackingpromote lysisBiological testingMetaboliteAntiendomysial antibodies
The invention provides a hair drug detection reagent and a detection device, and belongs to the technical field of drug detection, the reagent comprises a lysis solution and a specific antibody labeling detection reagent; the lysate comprises the following components: a surfactant, a chelating agent, a reducing agent, an enzyme preparation, a stabilizer, a preservative and a buffer solution. The device comprises the reagent. The components of the lysate have a synergistic effect, so that a hair sample can be quickly and effectively lysed, and drugs or metabolites thereof can be released.
Owner:ZHEJIANG XUANYI BIOTECHNOLOGY CO LTD

A microwave-assisted high-temperature cracking device

The present invention discloses a microwave-assisted high-temperature cracking device, including a microwave reactor and a feeding system. The microwave reactor includes a metal head, a metal cavity, a resonant cavity, a circular waveguide, a rectangular waveguide and a magnetron arranged from top to bottom; an absorbing material plate is fixed between the metal cavity and the resonant cavity, and a droplet micronizing feeder and a discharge port with adjustable spray height are provided on the metal head. The droplet micronizing feeder is used to spray fine droplets onto the upper surface of the absorbing material plate to perform a high-temperature cracking reaction. The present invention uses the metal head, the metal cavity and the absorbing material plate as a reaction site after being sealed by a flange, which not only realizes the flash heating process of the absorbing material plate but also effectively avoids the leakage of the reactant caused by the rupture of the reactor at high temperature. Through the circulation process of the material to be cracked in the outer jacket of the waveguide at the bottom of the device, it can not only preheat the material to be cracked but also reduce the heat transfer from the high-temperature metal reactor to the microwave generator, thereby realizing the effective circulation of energy.
Owner:ZHEJIANG UNIV OF TECH

Medical solid waste full-automatic treatment device adopting dynamic heat energy cracking

The invention relates to the technical field of solid waste full-automatic treatment devices, in particular to a dynamic thermal energy cracking medical solid waste full-automatic treatment device which comprises an equipment main machine frame, a machine frame stand column is fixedly installed on the outer side of the equipment main machine frame, and a barrel body lifting positioning mechanism is fixedly installed at the rear end of the machine frame stand column. And an inner pressure adjusting mechanism is rotatably mounted at the upper end of the upper cover hinge base, an equipment base is fixedly mounted in the equipment main rack, a power driving mechanism is fixedly mounted between a power motor and the lower end of the treatment cavity, and a cutter body angle adjusting mechanism is fixedly mounted at the output end of each telescopic rod. The staggered blades can complete efficient crushing, the contact area of solid waste and the high-temperature environment is increased, and the cracking efficiency is improved. And meanwhile, a cutter body angle adjusting mechanism can flexibly adjust cutting and stirring angles according to the type and hardness of the solid waste, precise adaptation of crushing and cracking is achieved, and the problems that due to a single treatment mode of traditional equipment, local harmlessness is not achieved, and the treatment period is long are solved.
Owner:AIXIN SMART MEDICAL TECHNOLOGY DEVELOPMENT (JIANGSU) CO LTD

Nematode enrichment method based on compound enzyme preparation

The invention discloses a nematode enrichment method based on a compound enzyme preparation, which is characterized in that a Viscozyme L enzyme and a Cellic cTec3 enzyme are compounded, so that the quick release of two nematodes, i.e., small-stem nematode and lethal meloidogyne, is realized. The optimal extraction conditions of the caenorhabditis caenorhabditis are as follows: the volume ratio of the Cellic cTec3 enzyme to the Viscozyme L enzyme is 1 mL / 40 [mu] L, the temperature is 25 DEG C, and the time is 4.5 h; for killing meloidogyne, the release efficiency is obviously increased along with the temperature rise, and the optimal conditions are as follows: the volume ratio of the Cellic cTec3 enzyme to the Viscozyme L enzyme is 2 mL / 80 mu L, the temperature is 35 DEG C, and the time is 4.5 h. High-efficiency enrichment operation can be realized by utilizing an optimized enzyme lysis solution system, the quantity of the extracted nematodes, namely, the causticia caerulea and the killing meloidogyne, is obviously increased compared with that of the nematodes extracted by the prior art, and microscopic morphology observation proves that the biological characteristics of the nematodes are kept complete and the state is good.
Owner:ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Industrial wastewater biological system sludge treatment system

The utility model discloses an industrial wastewater biochemical system sludge treatment system, it belongs to industrial wastewater treatment field, it will fluid power cavitation, thermal effect and alkaliolysis effect system integration, break through the limitation of single technology, realized the high -efficient sludge disintegration under low energy consumption. It mainly includes sludge pump, the sludge pump is connected with the pretreatment unit through valve no.
Owner:SHANDONG TIANDA TAIZE ENVIRONMENTAL PROTECTION TECH CO LTD

Biomass gasification tar-based porous carbon ball as well as preparation method and application thereof

The invention discloses a biomass gasification tar-based porous carbon ball as well as a preparation method and application thereof, and belongs to the technical field of carbon materials. In order to realize high-value resource utilization of gasified tar and prepare a micro-mesoporous adjustable hierarchical porous carbon material, the invention provides a preparation method of biomass gasified tar-based porous carbon spheres, which comprises the following steps: mixing tar, gasified slag, polyacrylonitrile and an organic solvent to form a uniform tar gasified slag suspension, dripping the suspension into a receiving liquid, and drying to obtain the biomass gasified tar-based porous carbon spheres. And after polymerization and curing, carbonizing to form a microporous structure, and then carrying out alkali washing and acid washing to obtain the gasified tar-based micro-mesoporous carbon spheres. According to the invention, the high-temperature thermoplasticity and medium-temperature crosslinking property of tar are utilized to realize pore forming, and template-free regulation and control of a microporous structure are realized; the gasified slag is used as a mesoporous template and a carbon precursor, the proportion of tar to the gasified slag is adjusted, regulation and control of a mesoporous structure are achieved, and the gasified tar-based carbon spheres which are low in cost and convenient and adjustable in micro-mesopores are prepared.
Owner:SICHUAN UNIV +1

A highly efficient cleaning device for municipal heavy oil road surfaces

The invention relates to the technical field of road cleaning, and specifically discloses an efficient cleaning device for municipal heavy oil road surface, comprising an equipment frame, wherein the top of the equipment frame is fixedly connected to a water storage tank, and the bottom of the equipment frame is provided with a dust suction component, a sweeping component and a cleaning component, wherein the dust suction component is fixedly mounted on the equipment frame, and the cleaning component comprises a telescopic mechanism, a stain cracking mechanism and a flushing mechanism; the telescopic mechanism, driven by the driving force provided by a motor, can drive the cracking block to fit more closely with the ground, and when encountering a protrusion on the ground, the cracking block will retract upward to adapt to different terrain conditions, and the protrusion is a block-shaped stain, and the cracking block will repeatedly scratch the block-shaped stain, and cooperate with the flushing component to achieve efficient cracking of the stain, thereby not only being able to effectively remove the stubborn stains on the heavy oil road surface, but also being able to greatly reduce the manpower input and time cost in the cleaning process, thereby more efficiently achieving the cleaning work of the heavy oil road surface.
Owner:SHENZHEN RUNHYUAN CONSTR ENG CO LTD

Thermal plasma nitric oxide treatment device

ActiveCN224404799Uefficient decompositionEfficient cracking
The utility model discloses a kind of thermal plasma nitrogen oxide processing devices, including processing device, the inside of processing device is separated into independent nitrogen oxide waste gas unit and waste gas reaction unit by fixed partition, the fixed partition is provided with multiple through channels and inlay thermal plasma torch, it is through two units, flame end is stretched to waste gas reaction unit, the other end is to nitrogen oxide waste gas unit, constitute gas only passage. By partition isolation, horizontal torch array and similar "double horn" flow channel, nitrogen oxide molecular bond is efficiently broken in short time;Simultaneously, material and structure integration break cooling dependence, so that industrial grade denitration equipment first has three breakthroughs of land occupation halving, multiple pollutants collaborative treatment and zero secondary pollution.
Owner:ZHONGKE YUNYUE (BEIJING) TECH DEV CO LTD

Method and kit for enriching low concentration pathogen nucleic acid in water body

PendingCN122648544AEfficient crackingPromote directional adsorption
The application belongs to the technical field of virus DNA enrichment. More particularly, it relates to a method and a kit for enriching low-concentration pathogen nucleic acid in water bodies. The enrichment cracking solution and the eluent constructed by specific proportion produce synergistic effect, efficiently crack the pathogen, and promote the target nucleic acid to be directionally adsorbed on the magnetic beads, so that the enrichment efficiency and detection sensitivity are significantly improved, the originally undetectable or weakly positive low-load samples are stably detected, and the false negative rate is greatly reduced. The enrichment reagent provided by the application is simple to prepare, the water sample does not need complex pretreatment, and the sample treatment and enrichment operation can be completed integrally on site. The method is excellent in anti-matrix interference, process simplification, reagent saving, operation speediness and on-site applicability, overcomes the technical bottleneck that low-load pathogens and nucleic acids easily cause false negatives in detection, helps the breeding farm to take disease prevention and control measures in time, and has good application prospect and value.
Owner:WENS FOODSTUFF GROUP CO LTD +1

A process for catalytic cracking of organosilicon high boilers

The application provides a method for catalytically cracking organosilicon high-boiling substances. The method comprises the following steps: contacting an organosilicon high-boiling substance raw material with a cracking catalyst to generate a cracking reaction; the reaction temperature of the cracking reaction is not lower than 500 DEG C; the cracking catalyst comprises a carrier and a metal active component loaded on the carrier, the metal active component at least comprises one or more of Fe, Zn, Ni, Ti and Mo, the mass content of the metal active component in the cracking catalyst is not less than 1%, the carrier at least comprises a molecular sieve, and the mass content of the carrier in the cracking catalyst is not less than 10%. The catalyst used in the cracking reaction of the application does not contain heavy metals, and has high selectivity to methyl chlorosilane monomers such as monomethyltrichlorosilane, dimethyldichlorosilane and trimethylmonochlorosilane, especially can effectively improve the selectivity of dimethyldichlorosilane, and has mild and efficient reaction conditions, high catalytic efficiency.
Owner:JIANGXI BLUESTAR XINGHUO SILICONE CO LTD +1

Lysate for nucleic acid extraction and application thereof

The invention discloses a lysate for nucleic acid extraction and application thereof, and relates to the technical field of molecular biology. The lysate is prepared from the following components in final concentration: 10 to 100 mM of alkaline substances, 0.05 to 6 w / v percent of a compound surfactant, 0.1 to 0.5 mg / mL of protease K and a compound penetrant, wherein the concentration of the alkaline substances is 10 to 100 mM; the concentration of the compound surfactant is 0.05 to 6 w / v percent; the concentration of the protease K is 0.1 to 0.5 mg / mL. The preparation is simple, and during use, only a small amount of sample needs to be added into the lysate, so that the PCR amplification requirement can be met; the lysate can rapidly and efficiently lyse a sample and release a large amount of high-quality DNA, has good compatibility with a PCR amplification system, and can be directly used for PCR amplification to obtain a clear and specific amplification product.
Owner:SANGON BIOTECH (SHANGHAI) CO LTD

Composite nanoparticles for bacterial extraction and lysis, and preparation method and application thereof

The application discloses a composite nanoparticle for bacterial extraction and lysis, and a preparation method and application thereof. The composite nanoparticle comprises urchin-like tungsten oxide with radial nanotips, Fe3O4 magnetic nanoparticles, target bacterial nucleic acid aptamer and lysozyme. The urchin-like tungsten oxide and the Fe3O4 magnetic nanoparticles form a composite framework with mechanical puncture and magnetic separation functions. The nucleic acid aptamer is used for specific binding of target bacteria, and the lysozyme is used for enzymatic hydrolysis of bacterial cell wall peptidoglycan. The urchin-like tungsten oxide nanotips puncture the outer structure of bacteria, promote the lysozyme to contact and lyse the cell wall, and realize rapid and specific capture and broad-spectrum and efficient lysis of bacteria. The composite nanoparticle can reduce complex sample interference, avoid damage of intracellular nucleic acid and protein caused by strong acid and alkali, high temperature or violent mechanical crushing, and is suitable for clinical infection diagnosis, food safety detection, environmental microbial monitoring and pretreatment before downstream nucleic acid detection.
Owner:UNIV OF JINAN

Method for preparing hydrogen by catalytic cracking of waste edible oil

This invention relates to a method for producing hydrogen from waste edible oils via catalytic cracking. The method involves precipitating and filtering the waste edible oils, then converting them into C1-C5 light hydrocarbons at 350℃-380℃ using a highly active, carbon-resistant, acid-resistant, and water-resistant large-pore hydrodeoxygenation catalyst and a highly efficient n-alkane cracking catalyst. The converted hydrocarbons are then passed through a fine desulfurization reactor and into a light hydrocarbon conversion furnace for cracking to produce hydrogen. Further conversion is performed in a medium-temperature shift converter to increase the yield. Finally, the hydrogen is purified using a PSA purification system to obtain high-purity hydrogen. This method features low reaction temperatures, minimal equipment requirements, and effectively avoids coking during the reaction process. The catalyst structure and synergistic effect of this invention result in high catalytic activity, improved catalytic efficiency, abundant C1-C5 light hydrocarbon components, and higher hydrogen purity.
Owner:HENAN JUNHENG IND GRP BIOTECH CO LTD

ZSM-5 nanosheet catalyst as well as preparation method and application thereof

PendingCN121990590AExcellent catalytic cracking effect of cross-linked polyethylenehigh value utilizationMolecular sieve catalystsTransportation and packagingPtru catalystLiquid hydrocarbons
The invention relates to a ZSM-5 nanosheet catalyst and a preparation method and application thereof, and belongs to the technical field of catalysts.According to the preparation method, the morphology of the catalyst is regulated and controlled through a specific silicon-aluminum ratio and a specific fluorine-aluminum ratio, so that the obtained ZSM-5 nanosheet catalyst has an excellent catalytic effect, and the catalyst has a good application prospect. The waste cable crosslinked polyethylene can be effectively degraded and recycled into a series of gaseous and liquid hydrocarbons, and high-value utilization of the waste cable crosslinked polyethylene is realized.
Owner:ELECTRIC POWER RES INST OF GUANGDONG POWER GRID CO LTD +1

Phage, phage preparation and application of phage preparation in reduction of nitrous oxide emission

The invention discloses a bacteriophage, a bacteriophage preparation and application of the bacteriophage preparation in reduction of nitrous oxide emission, the Latin name of the bacteriophage is Shigilla flexneri phage, the bacteriophage is named as B003, the bacteriophage B003 is preserved in the China Center for Type Culture Collection on July 1, 2024, and the preservation number is CCTCC NO: M 20241434; the bacteriophage B003 has a strong cracking effect on shigella flexneri, and the bacteriophage preparation can effectively reduce the discharge amount of nitrous oxide in the storage process of compost products, and is suitable for compost treatment of various organic wastes. By inoculating the shigella flexneri bacteriophage B003 preparation, nitrous oxide emission in the compost product storage process is reduced, the risk of greenhouse gas emission of the compost product is reduced, and the safety and environmental protection property of compost treatment are improved.
Owner:FUJIAN AGRI & FORESTRY UNIV

Method for synthesizing rhenium hexafluoride

PendingCN122010185Areduce contentAchieve in-situ eliminationRhenium compoundsPhysical chemistryMixed gas
The invention relates to the technical field of fluorine chemical industry, in particular to a synthesis method of rhenium hexafluoride. The method comprises the following steps: S1, preheating fluorine-nitrogen mixed gas to 50-100 DEG C in a first temperature zone; s2, introducing the preheated fluorine-nitrogen mixed gas into a second temperature zone to react with rhenium metal to generate mixed gas containing rhenium hexafluoride, wherein the temperature of the second temperature zone is 120-180 DEG C; s3, mixed gas containing rhenium hexafluoride is introduced into a third temperature zone and continues to react with rhenium metal, and the temperature of the third temperature zone ranges from 300 DEG C to 450 DEG C; s4, introducing the gas obtained in the step S3 into a fourth temperature zone, and controlling the temperature of the fourth temperature zone to be 200-300 DEG C, and S5, carrying out low-temperature collection on the gas generated in the step S4 through a cold trap to obtain the high-purity rhenium hexafluoride. Through temperature control in multiple areas, the impurity content is reduced, and the product purity is improved.
Owner:PERIC SPECIAL GASES CO LTD

Gas-solid two-phase flow control device for high-temperature methane catalytic cracking furnace

ActiveCN224271125UEfficient crackinguniform suspension stateHydrogenCarbon nanotubesPtru catalystThermodynamics
This invention relates to the field of methane cracking control equipment, particularly a gas-solid two-phase flow control device for a high-temperature catalytic cracking furnace of methane. The control device includes a reaction shell, an inlet pipe, an airflow baffle, a bottom microfiltration assembly, a flow-equalizing microporous assembly, a top microfiltration assembly, an exhaust pipe, a viewing window, a video monitoring system, and a flow control device. The airflow baffle and the bottom microfiltration assembly are installed at the bottom of the inner cavity of the reaction shell, while the top microfiltration assembly is installed at the top of the inner cavity of the reaction shell. This invention, by setting the bottom microfiltration assembly, the top microfiltration assembly, and the flow-equalizing microporous assembly inside the cracking furnace, and coordinating with the detection of the gas-solid two-phase flow inside the cracking furnace to adjust the airflow rate, achieves uniform suspension of catalyst particles in the high-speed airflow inside the cracking furnace. This enables efficient cracking of methane under the action of the catalyst and the deposition of nano-carbon, reducing the production cost of carbon nanotubes.
Owner:苏州福睿能源有限责任公司

A novel methicillin-resistant Staphylococcus aureus phage and its application

PendingCN122081244AEfficient crackingWide hostBiocideAntibacterial agentsDisinfectantStaphylococcus aureus
This invention relates to a novel methicillin-resistant Staphylococcus aureus (MRSA) bacteriophage and its applications. The invention provides a methicillin-resistant MRSA bacteriophage SP01, which is identified as a new species. This phage possesses highly efficient lytic ability against MRSA and has a broad host spectrum, showing sensitivity to multiple clinical isolates. Furthermore, phage SP01 exhibits excellent environmental stability, maintaining good activity within a temperature range of 4–40°C and a pH range of 7–11. In summary, this invention allows for the preparation of drugs, disinfectants, and bactericides for treating MRSA infections using phage SP01, addressing the problem of conventional antibiotic ineffectiveness and demonstrating broad application prospects.
Owner:SUN YAT SEN UNIV

Lysate for nucleic acid extraction and application thereof

The invention discloses a lysate for nucleic acid extraction and application thereof, and relates to the technical field of molecular biology. The lysate is prepared from the following components with final concentration: 20 to 150mM of alkaline substance, 0.5 to 5M of penetrant, 0.05 to 5.5 w / v percent of compound surfactant and 0.01 to 1mg / mL of protease K; the compound surfactant comprises nonylphenol polyoxyethylene ether and lauryl sodium sulfate. The lysate can efficiently lyse animal tissue cells, release high-quality genome DNA (deoxyribonucleic acid), provide sufficient templates for PCR (polymerase chain reaction) amplification and meet the requirement of direct PCR amplification; components in the lysate are stable, and the lysate is easy to store and transport and suitable for large-scale sample detection. In addition, the lysis buffer is suitable for lysis and PCR amplification of various animal tissue samples, and has a wide application prospect.
Owner:SANGON BIOTECH (SHANGHAI) CO LTD

Cell lysis microfluidic biochip and method based on surface acoustic wave principle

The invention belongs to the technical field of microfluidics, and particularly relates to a cell lysis microfluidic biochip based on a surface acoustic wave principle and a method. Wherein the interdigital electrode comprises bus bars and an electrode pair between the bus bars, the electrode pair comprises electrode finger strips and a suspension electrode structure, the electrode finger strips are fixed on the front bus bar and the rear bus bar, the suspension finger strips are arranged in door-shaped finger strips of the suspension electrode structure, and the front end, the middle end and the rear end of a micro-channel plate of the micro-channel are respectively provided with an inlet, a cracking chamber and an outlet. A micro-column baffle is arranged at the end, close to the outlet flow channel, of the cracking chamber, and magnetic particles are placed in the cracking chamber; the liquid drops are driven to move at a high speed through the acoustic microflow effect, cells in the liquid drops are driven to move and collide with particles rapidly, rapid and efficient cell lysis is achieved, and therefore the device is suitable for lysis analysis and biochemical detection of a small number of cell samples.
Owner:SICHUAN UNIV

A highly permeable cationic surfactant microbial lysate and its preparation method

PendingCN122081075AAvoid environmental hazardsBest wall breaking efficiencyMicroorganism lysisActive agentCell membrane
This invention belongs to the field of microbial cell lysis and extraction technology, and discloses a highly permeable cationic surfactant microbial lysis buffer and its preparation method. The lysis buffer employs a novel branched alkyl quaternary ammonium salt ester structure, introducing an α-hydroxy ester bond specifically recognized by extracellular lipase at the β-position of the quaternary ammonium nitrogen and the alkyl chain, achieving biodegradability after lysis. The preparation method employs a two-stage sequential lysis process: the first stage utilizes chelating agent microcapsules for slow release in a specific pH environment to pretreat the bacterial cells; subsequently, a stepwise surfactant addition strategy is used to activate and enhance cell membrane permeability; finally, efficient lysis is completed through lipase-catalyzed hydrolysis. This lysis buffer exhibits rapid penetration, high efficiency, targeted and controllable processing, and is environmentally friendly, suitable for the efficient extraction and downstream purification of bacterial proteins, enzymes, polysaccharides, and other intracellular products.
Owner:SHENZHEN YOUXIN INTERNET TECHNOLOGY CO LTD

A hybrid antibiotic enzyme for killing animal-derived pathogens and its application

ActiveCN115851690Bhigh purityEfficient crackingAntibacterial agentsAntimycoticsBiotechnologyHybrid protein
The present invention discloses a technical field involving antibiotic enzymes, and in particular, relates to a hybrid antibiotic enzyme for killing animal-derived pathogens and its application. The hybrid antibiotic enzyme includes a hybrid protein E1C1, and the hybrid protein E1C1 includes a catalytic domain E1 as shown in SEQ ID NO:2 and a binding domain C1 as shown in SEQ ID NO:3. The present invention also provides an application of a hybrid antibiotic enzyme in the preparation of a pharmaceutical composition for controlling microbial infection, and a pharmaceutical composition comprising the hybrid antibiotic enzyme as an effective ingredient. The hybrid antibiotic enzyme of the present application can quickly lyse common animal-derived pathogens Escherichia coli, Salmonella, Staphylococcus aureus and Helicobacter pylori, and can also efficiently kill common pathogens such as clinically isolated Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae.
Owner:KUNSHAN BOQING BIOTECHNOLOGY CO LTD