The application belongs to the technical field of
virus DNA enrichment. More particularly, it relates to a method and a kit for enriching low-concentration
pathogen nucleic acid in water bodies. The enrichment
cracking solution and the eluent constructed by specific proportion produce synergistic effect, efficiently crack the
pathogen, and promote the target
nucleic acid to be directionally adsorbed on the magnetic beads, so that the enrichment efficiency and detection sensitivity are significantly improved, the originally undetectable or
weakly positive low-load samples are stably detected, and the false negative rate is greatly reduced. The enrichment
reagent provided by the application is simple to prepare, the
water sample does not need complex pretreatment, and the sample treatment and enrichment operation can be completed integrally on site. The method is excellent in anti-matrix interference, process simplification,
reagent saving, operation speediness and on-site applicability, overcomes the technical
bottleneck that low-load pathogens and nucleic acids easily cause false negatives in detection, helps the breeding farm to take
disease prevention and control measures in time, and has good application prospect and value.