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147results about How to "Efficient cracking" patented technology

Method for extracting total RNA from plant tissue rich in polysaccharides and polyphenols and secondary metabolites

The invention provides an extration method of plant RNA by utilizing guanidinium isothiocyanate-chloroform extractive and Tris purification. The invention improves traditional guanidinium isothiocyanate method, adopts the two steps of chloroform extration after cracking and phenol-adding for purification, and respectively extract high-quality total RNA from the leaf blade and seed case of red-skinned pears, the leaf blade, fruit flesh and seed case tissues of grapes, the fruit of strawberries and the callus of arnebia euchroma. The extraction buffer liquid in the method contains guanidinium isothiocyanate, sodium dodecyl sarcosinate, sodium citrate, soluble polyvinylpyrrolidone and beta-mercaptoethanol, which can not only crack plant cells efficiently and release RNA, but also effectivelyinhibit the activity of RNA enzyme, and can also prevent the oxidation of phenolic compounds and suppress the interference of secondary metabolites. In the second step, Tris purification is utilized for crude extraction of RNA solution, which can eliminate the polysaccharides and protein which can coprecipitate with RNA. The invention is simple in operating steps, economic and practical, good in stability, and suitable for extracting high-quality total RNA from plant tissue rich in polysaccharides and polyphenols and secondary metabolites.
Owner:KUNMING UNIV OF SCI & TECH

Cracking of Olefins on Phosphorus Modified Molecular Sieves

The present invention provides a process for the catalytic cracking of an olefin-rich feedstock which is selective towards light olefins in the effluent, the process comprising contacting a hydrocarbon feedstock containing one or more olefins, with a catalyst made of a phosphorus-modified zeolite (A), to produce an effluent with an olefin content of lower molecular weight than that of the feedstock, wherein said phosphorous modified zeolite (A) is made by a process comprising in that order:
    • selecting a zeolite with low Si/Al ratio (advantageously lower than 30) among H+ or NH4+-form of MFI, MEL, FER, MOR, clinoptilolite, said zeolite having been made preferably without direct addition of organic template;
    • steaming at a temperature ranging from 400 to 870° C. for 0.01-200 h;
    • leaching with an aqueous acid solution containing the source of P at conditions effective to remove a substantial part of Al from the zeolite and to introduce at least 0.3 wt % of P;
    • separation of the solid from the liquid;
    • an optional washing step or an optional drying step or an optional drying step followed by a washing step;
    • a calcination step.
Said cracking of an olefin-rich feedstock is often referred in the following description and claims as OCP (Olefin Cracking Process).
The catalyst made of a P-modified zeolite (A) can be the P-modified zeolite (A) itself or it can be the P-modified zeolite (A) formulated into a catalyst by combining with other materials that provide additional hardness or catalytic activity to the finished catalyst product.
The zeolite with low Si/Al ratio has been made previously with or without direct addition of an organic template. The zeolite can be made with the help of seeds techniques but without template, the seeds could have been made with a template which means that the zeolite is made without direct addition of a template.
Owner:TOTAL RES & TECH FELUY

Method for dynamic water feeding and hole sealing using high water-absorbent resin

A method for dynamic water feeding and hole sealing using high water-absorbent resin is applicable to sealing of a borehole on which extraction has a great impact. Two ends of a hole-sealed section of the borehole are sealed. Fine mixed high water-absorbent resin powder wrapped in a piece of cotton cloth is disposed in the hole-sealed section of the borehole, a water injection pipe and a gas extraction pipe are inserted in the borehole, and an automatic feed water valve is used to inject water into the hole-sealed section. The fine mixed high water-absorbent resin powder that fills the hole-sealed section and surrounding cracks with the water flow rapidly swells up after soaking up water to fill the hole-sealed section, and maintains at a swelling pressure. After the powder swells up and hole sealing is completed, the extraction pipe is connected to an extraction apparatus to perform gas extraction. This method has advantages such as tight hole sealing, high automation degree, and dynamic hole sealing, thus effectively solving problems that pores around the borehole are poorly sealed, it is difficult to seal cracks newly produced by extraction and so on. Moreover, the powder for hole sealing is low in price, and therefore the hole sealing cost is low.
Owner:CHINA UNIV OF MINING & TECH
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