The invention discloses a method for removing viruses and endogenous and exogenous
bacteria from small yellow ginger, and relates to the technical field of
virus-free
seedling cultivation, and the method comprises the following steps: S1, selecting ginger blocks which grow robustly and fleshy and are free of damage and
plant diseases and
insect pests as explant materials, and placing the ginger blocks in a dark environment at 28-30 DEG C for accelerating
germination; s2, when the ginger buds grow to 2-3cm, selecting healthy and full ginger buds,
cutting off the ginger buds, washing the ginger buds with running water, and disinfecting the ginger buds; s3, under a sterile condition, transferring the disinfected ginger buds into a
primary culture medium, screening out the ginger seedlings with
bacteria by adopting a high-low temperature method, and ending culture when the ginger buds grow 1-3 coleoptiles; s4, stripping leaf coleoptiles of the screened healthy and
pollution-free ginger buds,
cutting off 1-3mm stem tips from growing points, and transferring the stem tips into a clumpy
bud induction culture medium for multiplication culture at the culture temperature of 20-25 DEG C and the illumination intensity of 2500
LUX for 8-15 days; s5, the clustered seedlings obtained after proliferation are
cut into single seedlings from the base parts, the single seedlings are placed in a rooting culture medium, and
virus-free plants are obtained and cultured for 30-45 d at the culture temperature of 22-25 DEG C and the illumination intensity of 3000
LUX.