This invention discloses a method and application for rapidly preparing humanized collagen using
umbilical cord mesenchymal stem cell material, belonging to the field of
biomaterial preparation technology. The key technical points of the method are as follows: S1,
processing and passage-expanding
umbilical cord tissue; S2, seeding the expanded
umbilical cord mesenchymal stem cell suspension into a culture dish, then adding DMEM / F12 medium containing
platelet lysis buffer for culture, followed by induction medium to obtain
extracellular matrix; S3, enzymatically hydrolyzing the
extracellular matrix, centrifuging the
hydrolysate to obtain the supernatant, adjusting the pH, allowing it to stand, centrifuging again to obtain the precipitate, reconstituteing the precipitate, purifying it, centrifuging again, and then lyophilizing it; S4, forming the formulation to achieve efficient
secretion and self-
assembly of collagen, while utilizing
extracellular matrix components (such as
fibronectin and
laminin) secreted by umbilical cord mesenchymal stem cells to assist collagen in forming an active product with a natural
triple helix structure.