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Clostridium butyricum active bacteria agent production method

A technology of Clostridium butyricum and a production method, which can be applied to bacteria and other directions, can solve problems such as raw materials cannot be reused, product quality is affected, and operations are complicated, and achieve the effects of low cost, stable product quality, and simple operation.

Active Publication Date: 2007-07-11
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The following patents: US5.292 523 (1994), US4.892 731 (1990), JP63-146825 (1986), JP61-6625 (1984), JP06-166825 (1994), JP05-227898 (1993), CN1144873C, CN1184308C relates to the preparation and application of Clostridium butyricum live bacteria agent, there are the following situations in these patents: 1, the cultivation of Clostridium butyricum species all adopts anaerobic culture tank or maintains its anaerobic by continuously feeding carbon dioxide or nitrogen environment, the raw materials cannot be reused, the operation is more complicated, and the cost is higher; 2. The nitrogen source mainly used in the medium is: beef extract, peptone, or yeast extract and its enzymatic hydrolysis products, and the cost is relatively high; 3. The obtained The number of fermentation bacteria is 10-15×10 8 cfu / ml bacterial liquid; 4. The culture is based on the spore formation rate maturity index, rather than the spore formation rate which can better reflect the product quality plus the mature spore shedding rate maturity index, so that the product quality is affected to a certain extent

Method used

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: the production method of a kind of clostridium butyricum active bacterial agent of the present invention:

[0033] 1. Preparation medium: the following medium is a preferred medium, but not limited to this medium, its raw materials and parts by weight are: soybean cake powder, i.e. fermented soybean meal 40-50, cornstarch 6-8, Glucose 18-22, yeast extract powder 1-3, tryptone 3-6, beef extract 0.3-1, NaHCO 3 1-3,K 2 HPO 41-3, raw materials should be calculated in grams, and the number of parts in ml of distilled water should be 800-1000; the specific ingredients are: soybean cake powder 45g, corn starch 7g, glucose 20g, yeast extract powder 2g, tryptone 5g, beef Extract 0.5g, NaHCO 3 1.5g, K 2 HPO 4 2g, CaCO 3 2g, distilled water 900ml;

[0034] 2. Disinfect and sterilize the culture tank, filter, sampler and other equipment and tools according to the routine, calibrate the pH electrode, dissolve the medium in the upper tank, install the pH elect...

Embodiment 2

[0041] Embodiment 2: different from above-mentioned embodiment 1:

[0042] 1. Preparation medium: 40 grams of soybean cake powder, 6 grams of corn starch, 18 grams of glucose, 1 gram of yeast extract powder, 3 grams of tryptone, 0.3 grams of beef extract, NaHCO 3 1 gram, K 2 HPO 4 1 gram, 800ml distilled water;

[0043] 2. Adjust the pH value of the medium during the dissolution process to: 7.1;

[0044] 3. The temperature for heating and sterilizing the culture medium in the fermenter is 100°C, and the time is 30 minutes;

[0045] 4. Inoculation of Clostridium butyricum seeds: After cooling the fermenter to 35°C, add 0.7g / 1000mL base material of inorganic salt, the inorganic salt is selected from MgSO 4 ·7H 2 O and CaCl 2 Two kinds, stir evenly during the adding process, then add liquid paraffin to a thickness of 1cm, insert Clostridium butyricum liquid seeds into 5ml / 1000ml base material, the selected Clostridium butyricum species preservation number is: CCAM 080039,...

Embodiment 3

[0049] Embodiment 3: different from above-mentioned embodiment 1:

[0050] 1. Prepare medium: 50g soybean meal powder, 8g corn starch, 22g glucose, 3g yeast extract powder, 6g tryptone, 1g beef extract, NaHCO 3 3g,K 2 HPO 4 3g, distilled water 1000ml;

[0051] 2. Adjust the pH value of the medium during the dissolution process to: 7.4;

[0052] 3. The temperature for heating and sterilizing the culture medium in the fermenter is 121° C., and the time is 10 minutes;

[0053] 4. Inoculation of Clostridium butyricum seeds: After cooling down to 45°C in the fermenter, first add 1.5g / 1000ml of inorganic salt, the inorganic salt is MgSO 4 ·7H 2 O, MnSO 4 ·H 2 O and CaCl 2 There are three kinds in total, stir evenly during the adding process, then add liquid paraffin to a thickness of 2.0cm, insert the liquid seed of Clostridium butyricum at 20ml / 1000ml, the selected Clostridium butyricum species preservation number is: CCAM 080040, but not Clostridium butyricum species li...

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Abstract

The invention discloses a manufacturing method of butyrate spindle bacillus microecological agent, which comprises the following steps: preparing culture medium; dissolving the culture medium; adjusting pH value; loading in the ferment tank; heating to sterilize the culture medium in the ferment tank; inoculating the butyrate spindle bacillus seed; controlling temperature to ferment; detecting the bacteria number of ferment liquid; adsorbing ferment liquid through plant fiber powder; drying; grinding; obtaining the powder agent.

Description

technical field [0001] The invention relates to a production method of a Clostridium butyricum live bacterial agent, which belongs to the technical field of microbes, in particular to a production method of a Clostridium butyricum microecological preparation. Background technique [0002] Clostridium butyricum (Clostridium butyricum), also known as butyric acid bacteria, is a species of Clostridium genus, mainly found in cheese, natural yogurt, animal manure, some soil and other environments. For a long time, Clostridium butyricum, as a probiotic preparation with a strong gut-regulating effect, has been widely used in medicines and feeds for the purpose of improving abdominal symptoms. At the same time, because Clostridium butyricum can activate the immune system, it is also used as an anti-tumor immune activator. In addition, Clostridium butyricum can also be used as a microbial fertilizer, which can promote growth and prevent diseases. [0003] As a microecological prepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 谢树贵赵述淼戴青梁运祥葛向阳陈正军胡咏梅王绩梅余霞田焕章
Owner HUAZHONG AGRI UNIV
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