77results about How to "Improve maturity rate" patented technology

The invention discloses a manufacturing method of butyrate spindle bacillus microecological agent, which comprises the following steps: preparing culture medium; dissolving the culture medium; adjusting pH value; loading in the ferment tank; heating to sterilize the culture medium in the ferment tank; inoculating the butyrate spindle bacillus seed; controlling temperature to ferment; detecting the bacteria number of ferment liquid; adsorbing ferment liquid through plant fiber powder; drying; grinding; obtaining the powder agent.

The invention relates to a method for improving in-vitro production efficiency of buffalo embryos, comprising the following steps of: respectively adding 10 muL / mL of insulin-transferrin-selenium (ITS) and 20 muL / mL of insulin-transferrin-selenium (ITS) to an oocyte maturing solution and a culture solution, and then carrying out in-vitro maturation and in-vitro culture. The method indicates that the in-vitro maturation rate of buffalo oocyte is effectively improved by adding 10 muL / mL ITS to the maturing solution, and the exhaustion ratio of a first polar body is up to 65.61%; and adding 20 muL / mL of ITS to the embryo culture solution is beneficial to the growth of early embryos of a fertilized buffalo, and the growing rate of a blastaea is up to 29.93%. the method has the advantages of improving the in-vitro maturation rate of the buffalo oocyte and the growing rate of the blastaea, thereby improving the in-vitro production efficiency of the buffalo embryos and providing a large number of high-quality embryos for embryo transplantation.

The invention discloses a production method of instant dry rice noodles. The method comprises the following steps of fine grinding of rice, rice soaking and washing, pulp grinding, blending of raw pulp and cooked pulp, viscosity increasing and standing, steaming of rice starch sheets and airing or drying; folding and ripening; strip cutting, shaping and microwave treatment; laminating; packaging and warehousing. The production method of the instant dry rice noodles is improved on the basis of a traditional processing method, so that the high-quality rice noodles which are rich in nutrition, smooth in taste and high in elasticity, do not cause soup burning during water boiling and cannot easily break during dry-frying are manufactured.

The invention discloses a porcine oocyte in vitro maturation medium and methods of preparation and culture. The medium comprises a TCM-199 base medium and the added porcine follicular fluid, fetal bovine serum, eCG, hCG, EGF and IGF-1. The porcine oocyte in vitro maturation medium of the invention can significantly increase the rate of maturation of porcine oocytes in vitro. Experiments confirm that after the oocyte collection and 42-44h maturation, the medium of the invention can have a polar body rate stabilized at 80% and a parthenogenetic embryo development rate up to 85%, and the entire maturation process does not require replacement of the medium and is easy to operate.

The invention discloses a method for hybridizing female verasper variegatus and male verasper moseri for breeding and fry cultivating, comprising the steps of selection of parents, parent fish cultivation and reproduction control, artificial insemination and incubation and cultivation of fish larvae and juvenile fish, wherein female verasper variegatus older than 3 years is selected to be used as a female parent, and male verasper moseri older than 2 years is selected to be used as a male parent; and the sexual maturity synchronization of the female parent fish and the male parent fish is promoted by means of temperature control, light control and nutrition enhancement. With respect to the artificial insemination, the insemination rate reaches 50%; the water temperature for incubation is (10+ / -0.5) DEG C, fish larvae can be incubated from inseminated eggs after 160 hours, and the incubation rate reaches more than 50%; and with respect to a feed series, L-type brachionus plicatilis and chlorella sp., artemia nauplii, and compound feed are respectively applied in different stages. The fish larvae (1-35 days in age) in an early stage are cultivated in a small water body (3m<3>) at the water temperature of (10+ / -0.5) DEG C; after 3 days, the temperature is raised by 0.5 DEG C every 2 days and is kept unchanged after reaching (15+ / -0.5) DEG C; and the fish larvae in a later stage are cultivated in a water body (30m<3>). After cultivating for 80 days, the survival rate of the fish larvae growing into the juvenile fish of 30mm in average is 20%. The method disclosed by the invention can be used for cultivating novel breeds and realizing scale breed reproduction.

The invention relates to a method for inducing gonad maturity and ovulation of a verasper variegates parent fish by implanting a slow-release hormone, which comprises two aspects of cultivating an optimally selected parent fish and adjusting temperature and light to promote the gonad development of the parent fish, and implanting the slow-release hormone for promoting a metrocyte of the parent fish to reach final maturity and ovulation. In the invention, the slow-release hormone is a salmon gonadotropin-releasing hormone analog (sGnRHa) and is coated with a safe and high-efficiency polymer slow-release transitive system (95 percent of cholesterol and 5 percent of cellulose); a female parent fish with gonad in fourth stage is selected by using a living body sampling method or a light table method; a special implanting gun is used for implanting in back muscles, and the effect time of the slow-release hormone lasts for 14-18 days. The method can enable the metrocyte of the verasper variegates parent fish to achieve final maturity and ovulation, has low duress on parent fish reproduction, high rate of parent fish survival and gonad maturity, high fertility rate being 72 percent and high hatching rate being 56.2 percent, and can acquire a bulk of high-quality oosperms. The invention is a set of complete control technique method, can stably regulate and control the development, the maturity and the ovulation of the gonad of the verasper variegates parent fish, and acquire high-quality oosperms.

The invention relates to a novel bovine oocyte in vitro maturation (IVM) culture solution, belonging to the field of embryo biotechnolory. In the invention, the routine IVM culture solution is added with amniotic epithelia or the supernatant of the culture solution thereof to establish a novel bovine immature oocyte IVM culture system, so as to improve the quality and maturation rate of the IVM bovine oocyte, thus solving the problem of low fertilization rate and low formation rate of blastula in the existing culture method.

The extracorporeal culture and mature method of immature oocyte from ovary tissue block includes selecting marrow mesenchyme stem cell MSC of female mouse as the feeder cell, compounding MSC culture liquid and agar and covering the culture rack, adding HCG in 3 IU / ml, and adhering the ovary tissue block on the rack to culture. In the co-culture system, estradiol, progestin and luteotopin contents are increased obviously, and after culturing, there is mature oocyte discharged. The method is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention relates to cell culture and particularly discloses an immature oocyte culture scheme which can promoting in-vitro maturation of immature oocytes. Specially, the scheme adopts follicular granulosa cells and a culture solution for culturing the follicular granulosa cells, wherein the culture solution for culturing the follicular granulosa cells contains CNP or its variants or analogues and an HDAC (histone deacetylase) inhibitor. The invention further provides an in-vitro maturation culture solution containing the CNP or its variants or analogues and the HDAC inhibitor and related compositions and application of a culture medium, the culture solution and the compositions in promotion of in-vitro maturation of immature oocytes.

The invention relates to a method for picea balfouriana somatic embryo generation and plant regeneration, which comprises steps of collecting picea balfouriana open pollination clonal cones, and separating immaturate zygotic embryos out for somatic embryogenetic culture, wherein the somatic embryogenetic culture comprises four stages, i.e. an embryogenetic callus inducing stage, an embryogenetic callus multiplicating stage, a somatic embryo maturity stage and a somatic embryo germination stage. Compared with the prior art, the method has the advantages that the seedling stage is greatly shortened, and natural maturing rate, seed maturation rate, differentiation rate and germination rate are all improved. The seedling culture method having short period, high multiplying rate, closely linked cultivation steps and low cost is provided for popularizing and planting picea balfouriana in a large scale for forestry production, is suitable for a rapid propagation technical system of picea balfouriana cell engineering and satisfies the requirements of markets on picea balfouriana.

The invention discloses a method and system for treating residual sludge by solar energy thermal radiation oxygen-enriched fermentation. The method comprises the following steps of: I, mixing solid wastes, liquid wastes, livestock manure and a dry admixture in the mass ratio of 8:1:1-7:1:2; II, performing thermal radiation oxygen-enriched fermentation at the fermentation temperature of between 50DEG C and 70 DEG C for 7 to 10 days until the moisture ratio of the mixture falls below 30 percent; and III, mixing and packaging. By adopting the method, thermal radiation oxygen-enriched fermentation is performed on residual sludge produced by urban sewage plants, so that power consumption in a fermentation process is lowered, fermentation period is shortened, floor area and running cost are reduced, and the problem of secondary pollution in the fermentation process of residual sludge is solved. The method is suitable for treating residual sludge such as sludge produced by urban sewage treatment plants, screen underflow produced by screening household garbage, livestock breeding manure, solid wastes of crop farming and gardens.

The present invention relates to a method for producing a probiotic agent of live Clostridium butyricum, belonging to the field of microbial technology and specially relates to a solid fermentation method for producing the probiotic agent of live Clostridium butyricum. The method comprises the steps of: employing the clostridium butyricum as a fermentation strain which is inoculated on an optimized solid fermentation medium, fermenting under conditions of isolation from air and a temperature of 35-42 DEG C for 48-72h, drawing off and determining a viable cell density, drying, crushing, and packaging to finished products. The method of the invention is advantageous in that: the production process is simple and stable, and easy for promotion; soybean meal and corn flour are used as main ingredients for the medium, greatly reducing the cost of production; the viable cell density is high, the maturation rate of spores is high, stable product quality; and the product quality is stable; and the fermentation product can be directly dried for use without purification, reducing costs and improving the product quality.

The invention relates to an oxygen-enriched biological treatment method and an oxygen-enriched biological treatment system of solid liquid waste. The method comprises the following steps of: 1, mixing the solid liquid waste, livestock and poultry culture excrement and a dry doped material according to the mass ratio of the solid liquid waste to the livestock and poultry culture excrement to the dry doped material of 8:1:1 to 7:1:2, and adding a mineral doped material in an amount which is 0.1 to 5 percent of the weight of the dry doped material; 2, performing groove type biological oxygen-enriched fermentation at the temperature of between 50 and 70 DEG C for 4 to 8 days, so that the water content of the materials is reduced to below 55 percent through fermentation; 3, performing windrow type biological fermentation for 4 to 8 days until the water content is reduced to below 30 percent; and 4, mixing and packaging the materials. By the method and the system, the fermentation process is in the oxygen-enriched state all the time, so the fermentation speed is increased, occupied area and operating cost are reduced, and the problem of secondary pollution of urban solid liquid waste is solved. The method and the system are applicable to treatment of the solid liquid waste such as sludge generated by urban sewage treatment plants, household waste, livestock and poultry culture excrement, crop cultivation and garden solid waste and the like.

The present invention is feeder cell for extracorporeal culture of immature oocyte and features that marrow mesenchyme stem cell MSC of female mouse is used as the feeder cell. The feeder cell is easy to obtain, can secrete several kinds of cell factors and growth factors, promote over 90 % of the immature oocyte to become mature and promote the growth, ovulation and maturing of the ovarian follicles in the ovary tissue block under extracorporeal culture. The present invention is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention provides an organic fertilizer and a preparation method thereof. The organic fertilizer comprises the following raw materials in percentage by weight: 10% to 30% of coal gangue, 30% to 60% of coal slime, 10% to 30% of fly ash, 5% to 20% of livestock manure, and 1% to 10% of animal waste. The organic fertilizer provided by the invention has a good fertilizing effect and can improve the structure of land to a certain extent.

The invention discloses a method for generating a hybrid liriodendron somatic embryo by using jasmonic acid methyl ester. The method comprises the following steps: seed sterilization, induction of embryonic callus, establishment of an embryogenic cell suspension line, acquisition and transition of liquid single cells, and induction of generation of the hybrid liriodendron somatic embryo. The method is characterized in that when generation of the hybrid liriodendron somatic embryo is induced, the jasmonic acid methyl ester and ABA are added in the culture medium, or the jasmonic acid methyl ester is independently added in the culture medium. According to the method for generating the hybrid liriodendron somatic embryo by using the jasmonic acid methyl ester, a plant growth regulator MeJa is added, the inductivity of the somatic embryo is increased effectively, the abnormal rate is reduced, the generating efficiency of the hybrid liriodendron somatic embryo is improved further, the practicability is good, and establishment of a hybrid liriodendron rapid propagation system is facilitated.

The invention discloses a total artificial propagation method of opsariichthys bidens. According to the method, parent fish is selected from first-generation and second-generation artificially cultivated groups of wild opsariichthys bidens subjected to artificial domestication and breeding, artificial cultivation is performed until opsariichthys bidens gonad develops maturely, and therefore the parent fish individuals have good maturation synchronism, and the maturation rate is higher; then hormone HCG is used for spawning induction, the spawning induction effect is good, and the spawning induction rate is high; physiological saline is used for activating spermatozoa during artificial insemination, the fertilization effect is improved, and the fertilization rate is higher; and fertilized eggs are put in an incubation frame in an incubation device, hatching is carried out under temperature control conditions, and therefore the hatching effect is improved and a high hatching rate is achieved. According to the method of the present invention, improvement and innovation are carried out on the prior art to realize the full artificial propagation of the opsariichthys bidens. The method focuses on technical links and refinement of technical parameters so as to facilitate popularization and use. The technical method of the present invention is verified by multiple production, and the operability and practicality are high.

The invention relates to application of cordycepin in in-vitro maturation culture of oocytes of small pig follicles, which belongs to the field of auxology of animals. Degrees of polyadenylation of the maternal genes consisting of cyclinB1, C-mos, GDF9, BMP15 and Cdc2 in oocytes determine expression levels of the genes, which has certain promotion effects on the recruitment process where small follicles become dominant follicles and on maturation of oocytes. The expression levels of the genes consisting of C-mos, GDF9 and BMP15 play a leading role in maturation of the oocytes of small follicles. Addition of a 3'-dA inhibitor can improve the expression levels of the genes consisting of C-mos, GDF9 and BMP15, so the maturation rate of the small follicles is increased. The technology of in-vitro maturation culture of oocytes is expected to be popularized and applied in research fields like transgenic technology, nuclear transplantation technology and embryonic stem cell technology.

A disclosed aseptic agricultural organic fertilizer is prepared from the following raw materials in parts by weight: 30-45 parts of rice husk ash, 20-40 parts of a xylitol by-product, 15-30 parts of coal gangue, 40-50 parts of coal slime, 10-20 parts of kaolin, 7-14 parts of quartz, 8-16 parts of mica, 10-20 parts of dry chicken manure, 8-16 parts of dry pig manure, 5-15 parts of calcium magnesium phosphate fertilizer, 10-15 parts of corruption bacteria and 20-40 parts of waste vegetable leaves. The provided aseptic agricultural organic fertilizer helps to obtain high crop output and good crop quality and save cost, is free of public hazard, generates relatively good fertilizer effect and gives play to certain improving effect on soil structure by combining conventional coal resource and livestock and poultry manure.

The invention discloses a composition for improving ovarian function and improving ovum and embryo quality and application thereof. The invention provides the composition containing nicotinamide mononucleotide, melatonin and coenzyme Q10, the ovulation rate of old animals can be remarkably improved, the maturation rate and fertilization rate of ova and the development rate of early embryos are increased, and the composition has great significance in prevention and treatment of female infertility and in-vitro production of animal embryos. The three active ingredients in the composition are endogenous substances of a biological organism, and side effects cannot be generated from published literatures, so that the safety of the composition serving as a medicine, a health care product or a biological preparation has very high safety.

The invention relates to a CIK cell expansion method, comprising the following steps: obtaining mononuclear cells, using a CIK cell inducer to induce and culture the mononuclear cells until the CIK cells are harvested; placing the CIK cells in a serum-free medium In vitro expansion culture; wherein, the serum-free medium contains PPP, IL-2, IL-12 and IL-27. It can not only improve the expansion efficiency and maturation rate of CIK cells, but also increase the CD3 + and CD56 + Increase the number of CIK cells and increase the killing activity.

The invention relates to a method for somatic embryogenesis and plant regeneration of slash pines. The method for somatic embryogenesis and plant regeneration of the slash pines comprises the steps of(1) collecting slash pines cones and isolating immature zygotic embryos; (2) inducing embryogenic callus formation; (3) proliferation of embryogenic callus; (4) curing and cultivating cotyledon embryos with germination potential; (5) sprouting and cultivating to obtain sprouted seedlings and (6) hardening seedlings and transplanting. An induction medium is a high-concentration nitrate nitrogen medium. The total concentration of nitrate ions in the culture medium is 13.8 to 14.6 mM; hormone components in the induction medium are prepared from 2.5 to 3 mu M of 6-benzyl aminopurine, 10.5 to 11 mu M of naphthylacetic acid, 2.5 to 3 mu M of Kinetin, 18.7 to 19.2 mu M of abscisic acid, and 0.8 to 1.2 mu M of brassinolide. The method has a high induction rate of embryogenic callus, somatic embryo maturation rate and germination rate, and the plant survival rate after transplanting is high.

The invention belongs to the technical field of chicken cultivation and in particular relates to a feeding method capable of improving growth performance of broiler chicken 1-21 days old. The feeding method comprises the following concrete steps: (1) feeding young chicken with feed everyday, namely feeding the young chicken with feed for five times everyday; and (2) feeding the young chicken with nutrient solution everyday, namely feeding the young chicken with the nutrient solution twice everyday. The feeding method provided by the invention has the advantages that content of proteins, DNA and RNA of intestinal mucosae is increased, intestinal villi are heightened, intestinal walls are thickened, small intestinal mucosa enzyme activity is improved, intestinal development is further promoted, growth of the intestinal villi is promoted, villi are heightened, and recesses are shallowed, so that quantity of mature cells of intestinal epitheliums is increased, and maturation rate is increased, capability of digesting and absorbing nutrient substances is enhanced, feed utilization rate is improved, and the broiler chicken can be grown rapidly; meanwhile, metabolism of microorganisms is accelerated, structure of a flora is adjusted, intestinal microecological balance is effectively maintained, intestinal conditioned pathogens are inhibited, nutrition is provided, body immunity is enhanced, and robust growth of the young chicken is promoted.

The invention provides an oocyte in-vitro maturation culture solution additive and application thereof. The additive is dimethyl alpha-ketoglutarate. The oocyte in-vitro maturation culture solution additive disclosed by the invention is added into an oocyte in-vitro maturation culture solution and is used for culturing oocytes in vitro; according to the method, the maturation rate and quality of the oocytes are remarkably improved, the in-vitro fertilization rate and blastocyst rate are remarkably improved, the ROS content of the oocytes after oxidative damage is remarkably reduced, and the GSH content in the oocytes after oxidative damage is remarkably improved. The success rate and quality of embryo engineering can be improved when the method is applied to a livestock assisted reproduction technology.

The invention relates to a culture method of dendritic cells. The culture method includes the following steps that a single karyocyte of a dendritic cell precursor is obtained, culturing is conducted for 4-7 days through a first inductive agent, and immature dendritic cells are obtained; then, a second inductive agent is added to culture the immature dendritic cells till the dendritic cells are mature; the first inductive agent comprises rhGM-CSF and rhIL-4; the second inductive agent comprises rhTNF-alpha, tomato fat-soluble extract or tomato water-soluble extract. The invention further relates to the dendritic cells obtained through the method. Compared with the prior art, the method improves cell activity of the dendritic cells, the value-added ratio and maturing rate of the dendritic cells are raised, and thus the antigen presentation property of the dendritic cells is improved.

The invention discloses a water-soluble fertilizer special for cultivating fruits and vegetables. The water-soluble fertilizer comprises the following components in parts by weight: 2-5 parts of nitrogen, 12-15 parts of phosphorus pentoxide, 14-28 parts of a micro-porous particle carrier, 10-15 parts of boron, 20-25 parts of iron, 2-8 parts of magnesium oxide, 5-8 parts of potassium humate, 2-6 parts of citric acid, 12-15 parts of amino acid, 3-8 parts of vitamin, 6-10 parts of fructose, 3-5 parts of a growth catalyst, 8-15 parts of sodium potassium tartrate tetrahydrate, and 10-20 parts of ethylene glycol. After the fertilizer disclosed by the invention is applied to a fruit and vegetable garden, the fruiting rate is increased by 70% or more; the yield is increased by 50-60%; fruits and vegetables are matured ahead of time of 8-10 days; the fruits are large and regular in shape; fruit surface colors are converted from orange yellow to orange red; the fruits are smooth in peel, sweet in taste, crisp, easy to melt and good in color, aroma and taste; and the commodity rate is 90% or more. The fertilizer disclosed by the invention is prepared from nitrogen, phosphorus pentoxide, the micro-porous particle carrier, boron, ion, magnesium oxide, potassium humate, citric acid, amino acid, vitamin, fructose, the growth catalyst, sodium potassium tartrate tetrahydrate and ethylene glycol according to a proper proportion; and therefore, side effects cannot be caused.

The invention relates to the technical field of organic fertilizers and particularly relates to a selenium-enriched organic fertilizer for sugar oranges and a preparation method of the selenium-enriched organic fertilizer. The organic fertilizer is mainly prepared by fermenting the following raw materials: excrements of livestock, saw dust, straws, a leaf weed powder, a melon vine powder, waste proteins, animal hair, rare earth elements, organic acids, cell-membrane stabilizing enzymes, plant hormones, selenium dodecyl, diaryl selenium, calcium selenite, cardamine hupingshanesis, seaweeds, garlic, astragalus, konjak, tea, spirulina, chequer-shaped indocalamus leaves, microbes and the like. The selenium-enriched organic fertilizer for sugar oranges is rich in nutritional ingredients and hasthe effects of killing bacteria and killing insects, the plant diseases and insect pests are reduced, the utilization ratio of pesticides and fertilizers is reduced, the acre yield, soluble solid content and selenium content of the sugar oranges are obviously increased, and the quality of the fruits is improved. Therefore, the economic benefits of sugar orange planting are improved, and the preparation process is simple and easy to produce.

The present invention relates to a DC inducer, comprising: rhGM-CSF, GM-CSF, IL-4, rhIL-4 and IL-13. The present invention further relates to the application of the above-mentioned DC inducer in the process of inducing DC maturation. Compared with the prior art, the DC inducer provided by the present invention can increase the cell activity of dendritic cells, increase the proliferation rate and maturation rate of dendritic cells, and further improve the antigen presentation of dendritic cells.

The invention discloses a method for returning autumn rice straw to field in cold regions. The method comprises the following steps of preparing straw degradation indigenous mixed bacterium liquid: mixing rice straw with paddy soil, performing watering, soaking the straw, besides, enabling soil to be under the damp condition, after 10-15 days, placing collected rotten straw in cellulose liquid culture mediums, performing culture to obtain mixed bacterium mother liquid, adding the mixed bacterium mother liquid to the cellulose liquid culture mediums, and performing enlarged cultivation to obtain the mixed bacterium liquid; and performing straw returning to fields for decomposition: spraying the mixed bacterium liquid on the surfaces of the crushed and soaked straw, besides, sprinkling organic fertilizers, performing rotary tillage, and performing watering. By the method, the decomposition rate is promoted by 240%-330%, the nutrient releasing rate is promoted by 57%-210%, and the methodhas important significance in straw resource utilization and increase of paddy soil quality.

The invention discloses a red fluorescence protein, a fusion protein, a separated nucleic acid, a carrier and an application, and belongs to the technical field of the biomedical optics and molecular imaging. Compared with the fluorescent protein mRuby3, the provided red fluorescence protein comprises the following amino acid mutation sites: combinations of one or more of N8E, R10P, E114V, V116I, T177E and R179K. The provided red fluorescence protein has photophysical properties, such as higher brightness, maturing rate and extinction coefficient, can be used for protein marking and imaging, or used as fluorescence resonance energy transfer acceptor or donor for application. The red fluorescence protein has better fluorescence resonance energy transfer efficiency.