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Oocyte in-vitro maturation culture solution additive and application thereof

A technology for in vitro maturation culture and oocyte, which is applied in the field of oocyte in vitro maturation medium additive field, can solve the problems of oocyte quality decline, poor embryonic development, etc., to improve in vitro fertilization rate and blastocyst rate, improve maturation rate and quality, and the effect of reducing ROS content

Pending Publication Date: 2022-04-29
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Oxidative stress negatively affects the developmental potential and mitochondrial function of mammalian oocytes resulting in decreased oocyte quality and subsequent poor embryonic development due to an imbalance between the production and elimination of intracellular ROS

Method used

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  • Oocyte in-vitro maturation culture solution additive and application thereof
  • Oocyte in-vitro maturation culture solution additive and application thereof
  • Oocyte in-vitro maturation culture solution additive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The oocyte maturation medium in vitro of this embodiment comprises:

[0043] Oocyte basal culture medium (TCM-199 purchased from GIBCO) with a concentration volume ratio of 75% to 85%;

[0044] 0.3mM sodium pyruvate;

[0045] 2mM glutathione;

[0046] 1 μg / mL estradiol;

[0047] Volume fraction 10% fetal bovine serum;

[0048] 100 μM cysteine;

[0049] 10ng / mL epidermal growth factor;

[0050] 10IU / mL pregnant horse serum gonadotropin;

[0051] 10IU / mL human chorionic gonadotropin;

[0052] Dimethyl α-ketoglutarate was added so that the final concentration of dimethyl α-ketoglutarate was 3 mM.

Embodiment 2

[0054] In this example, except that the final concentration of dimethyl α-ketoglutarate is 2.8 mM, the others are the same as in Example 1.

Embodiment 3

[0056] In this example, except that the final concentration of α-ketoglutarate dimethyl is 3.2 mM, the others are the same as in Example 1.

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Abstract

The invention provides an oocyte in-vitro maturation culture solution additive and application thereof. The additive is dimethyl alpha-ketoglutarate. The oocyte in-vitro maturation culture solution additive disclosed by the invention is added into an oocyte in-vitro maturation culture solution and is used for culturing oocytes in vitro; according to the method, the maturation rate and quality of the oocytes are remarkably improved, the in-vitro fertilization rate and blastocyst rate are remarkably improved, the ROS content of the oocytes after oxidative damage is remarkably reduced, and the GSH content in the oocytes after oxidative damage is remarkably improved. The success rate and quality of embryo engineering can be improved when the method is applied to a livestock assisted reproduction technology.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an oocyte maturation culture medium additive in vitro and its application. Background technique [0002] The application of assisted reproductive technology includes in vitro maturation, in vitro fertilization, embryo transfer, etc. This technology is beneficial to the development of animal husbandry and the clinical treatment of male and female infertility. Despite some developments in assisted reproductive technology over the past few decades, there has been no substantial improvement in the outcome of embryo transfer in livestock. The quality of in vitro cultured oocytes is a key factor in determining embryo development, but the maturation efficiency and quality of in vitro cultured livestock oocytes have not been greatly improved so far. Reactive oxygen species (reactive oxygen species, ROS) is an important factor affecting the efficiency of oocyte culture in vitro. Th...

Claims

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Application Information

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IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2500/30
Inventor 戴雁峰郝玉春王嘉鹏
Owner INNER MONGOLIA UNIVERSITY
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