229 results about "Blastocytes" patented technology

The invention discloses a culture solution for reducing lipid of animal embryos before freezing as well as a preparation method and application of the culture solution. The culture solution is prepared by adding bezafibrate and cholic acid into an NCSU23 culture solution. The invention also discloses a preparation method of the culture solution, which comprises the following steps: preparing an NCSU23 culture solution, and adding bezafibrate and cholic acid into the NCSU23 culture solution. Compared with a conventional NCSU23 culture solution, the culture solution provided by the invention has the advantages that the added bezafibrate and cholic acid have a synergistic lipid-lowering effect, so that the content of lipid in an embryo can be remarkably reduced, and the survival rate and the blastocyst hatching rate after the embryo is frozen and unfrozen are finally effectively improved. The culture solution provided by the invention has application prospects in the aspects of animal embryo lipid reduction, frozen embryo survival and development and the like.

The invention discloses an image detection and evaluation method for an unfrozen form of a fertilized egg embryo. The method comprises the following steps: acquiring a time sequence image sequence after the fertilized egg embryo is unfrozen; performing image standardization processing on the time sequence image sequence to generate a standardized embryo image set; extracting multi-dimensional embryo quality parameters from the standardized embryo image set; wherein the multi-dimensional embryo quality parameters comprise a morphological static parameter set, a dynamic change parameter set and a texture feature parameter set; generating a blastocyst formation rate based on the multi-dimensional embryo quality parameters; outputting a third-level embryo quality evaluation result according to a comparison relationship between the blastocyst formation rate and a preset threshold interval; wherein the three-stage embryo quality evaluation result comprises an excellent embryo grade, a qualified embryo grade and an abnormal embryo grade. The method has the following advantages and effects: full-automatic and multi-dimensional quantitative evaluation of the thawing process of the unfrozen embryos is realized, so that the scientificity of embryo screening and the success rate of clinical pregnancy are improved.

The invention provides an embryo three-dimensional reconstruction method and device, electronic equipment and a storage medium, and relates to the technical field of embryo quality evaluation. Comprising the following steps: acquiring a multi-focal original plane image of a blastocyst to be reconstructed based on a time difference incubator; adopting a pre-trained image frame interpolation model to perform iterative frame interpolation processing on the multi-focal original plane image to generate a multi-focal extended plane image corresponding to each iteration; according to the multi-focus original plane image and the multi-focus extended plane image corresponding to each round of iteration, adopting an auxiliary circle marking algorithm to mark a blastocyst main body area in each focus original plane image and each focus extended plane image; respectively extracting mask images of a plurality of inner cell clusters and mask images of a plurality of trophoblast cells on the basis of marking results; and performing three-dimensional reconstruction according to the extracted mask images of the internal cell clusters and the mask images of the trophoblast cells to obtain curved surface models of the internal cell clusters and curved surface models of the trophoblast cells. The method improves the reconstruction precision.

The invention provides biopsy liquid and application thereof in pig blastocyst laser cutting, and belongs to the technical field of animal biology. The invention provides a biopsy solution and a preparation method thereof, the biopsy solution can be applied to pig blastocyst biopsy by laser cutting, the problem that at present, no adaptive biopsy solution exists for laser cutting of pig blastocyst is solved, and the invention further provides a method for laser cutting of pig blastocyst by using the biopsy solution. The problems of embryo cell injury, embryo development potential damage and the like possibly caused by laser cutting of the pig blastocyst in the prior art are solved. The biopsy liquid and the use method thereof can be efficiently and accurately applied to acquisition of pig blastocyst trophoblast cells, and are low in cost and good in repeatability.

The invention belongs to the field of antibody development, and discloses a screening method and application of a human-mouse protein high homologous target antibody based on a fully humanized antibody mouse. Aiming at the problem of weak antibody response caused by immune tolerance of human-mouse high homologous targets (protein homology is greater than or equal to 95%), the following scheme is provided: in embryonic stem cells (ES cells) of HUGO-Mabfully humanized antibody transgenic mice, a mouse target gene (such as ACVR2A) is knocked out through a Turbo Knockout technology, and homozygous knockout ES clones are screened; carrying out microinjection on the clones to the whitened B6 mouse blastocyst, and transplanting a pregnant mouse to obtain a Founder mouse; the Founder mouse is subjected to target antigen immunization for more than or equal to 4 times (the Freund's complete adjuvant is used for the first time), and the titer of the serum antibody is detected. According to the invention, 100% homozygous knockout chimeric efficiency is realized in the Founder stage, the mouse construction period is shortened from traditional 8-10 months to 3-4 months, and the diversity and affinity of the antibody are significantly improved (titer reaches 1: 729,000). The obtained antibody can be used for preparing medicines for treating tumors or autoimmune diseases.

The invention provides an improved blastocyst culture solution as well as a preparation method and application thereof, and relates to the field of embryonic cell in-vitro culture. Based on a formula of a conventional blastocyst culture solution, colony stimulating factor-1 (CSF-1) and a transforming growth factor-alpha (TGF-alpha) are added, and the colony stimulating factor-1 and the transforming growth factor-alpha are dissolved in the conventional culture solution according to the concentration of 0.02-0.06 mmol / L and the concentration of 0.01-0.05 mmol / L; the obtained improved blastocyst culture solution can improve the mulberry embryo formation rate or blastocyst formation rate in in-vitro culture, promote the development of low-quality embryos and improve the mulberry embryo quality or blastocyst quality, and can be used for preparing medicines for promoting embryo implantation or subsequent growth and development.

The invention belongs to the field of biomedical image processing, and relates to a blastocyst-stage embryo morphological quality evaluation method based on multi-modal information, which comprises the following steps: acquiring an embryo microscopic image and clinical data of a patient, and acquiring a trained embryo morphological quality evaluation model, based on the trained embryo morphological quality evaluation model, performing knowledge distillation according to the embryo microscopic image and clinical data of the patient to obtain a student model; obtaining an embryo microscopic image and clinical data of a patient, and inputting the embryo microscopic image and the clinical data of the patient into the student model to obtain an evaluation result; the embryo morphological quality evaluation model comprises a multi-modal feature extraction module, a comparison module, a multi-modal feature fusion module and a multi-task embryo quality rating module; according to the method, the comparison learning mechanism is utilized to align the embryology evaluation text information with the image features, so that the evaluation accuracy is improved.

The invention discloses a preparation method and application of a conditioned culture solution for improving the development rate of bovine in-vitro embryos. The preparation method comprises the following steps: culturing immortalized bovine endometrial epithelial cells by using a DMEM / F12 cell culture solution containing 10% FBS, collecting the cell culture solution in the culture process, and filtering to obtain an immortalized bovine endometrial epithelial cell conditional culture solution, namely the conditional culture solution capable of improving the development rate of the bovine in-vitro embryos. The prepared conditioned culture solution can simulate a liquid environment of an in-vivo environment, is used for culturing in-vitro embryos such as parthenogenetic activated embryos and in-vitro fertilized embryos, improves the cleavage rate and blastocyst rate of the in-vitro embryos and improves the development rate of the in-vitro embryos of cattle.

The present invention relates to a blastocyst quality evaluation method and system. The method comprises: inputting a blastocyst multi-focus image into a pre-trained deep learning model to obtain a blastocyst development stage and quantitative scores for ICM and TE; obtaining a blastocyst quality quantitative score by means of the quantitative scores for ICM and TE; and selecting an optimal blastocyst by means of the blastocyst development stage and the blastocyst quality quantitative score. According to the present invention, the quality of blastocysts of the same grade is distinguished, thereby further quantifying the overall quality of an embryo.

The invention provides a trophoblast cell characteristic analysis method and electronic equipment, and relates to the technical field of embryo evaluation. The method comprises the following steps: carrying out space quadrant division on a target blastocyst three-dimensional model obtained by three-dimensional reconstruction to obtain a plurality of sub-quadrants; determining polar trophoblast cell information and wall trophoblast cell information according to the internal cell mass information in each sub-quadrant; determining multiple fusion feature data according to the trophoblast cell information, the polar trophoblast cell information and the wall trophoblast cell information in each sub-quadrant; and according to the fusion feature data and the basic feature data, carrying out embryo development result analysis to obtain estimated development result information of the target blastocyst. According to the scheme, the spatial structure features of the trophoblast cells are fully mined to obtain the fusion features, and the embryonic development result can be analyzed more deeply based on the fusion features, so that the accuracy of embryonic development result evaluation is improved, and clinical selection of embryos with higher development potential is assisted.

The invention discloses a pharmaceutical composition for delaying oocyte senescence and application thereof, and relates to the technical field of biological medicine, and the pharmaceutical composition comprises alpha-ketoglutaric acid, a bicarbonate buffer solution, an energy metabolism substrate, polyvinyl alcohol, hormone, a growth factor and insulin-transferrin-selenium. The pharmaceutical composition for delaying oocyte aging disclosed by the invention is beneficial to improving the maturation rate of oocytes, reducing the cytoplasm fragmentation rate and the cleavage fragmentation rate, and improving the cleavage rate and the blastocyst rate; according to the invention, the content of beta-galactosidase is reduced, the function of mitochondria in the aging process after ovulation of the mature oocyte is improved, the membrane potential of the mitochondria in the aging process after ovulation of the mature oocyte is stabilized, the activity of the mitochondria is improved, and the normalization of RNA methylation modification in the aging process after ovulation of the mature oocyte is promoted.

Provided are a method for producing a porcine blastoid from porcine embryonic stem cells (pESC), and a blastoid obtained by the method. Also provided are media for inducing the pESC to form blastula-like embryos and media for producing and culturing the pESC.

The invention relates to a method for inducing mouse trophoblast stem cells in vitro. According to the method, mouse embryonic stem cells are used as starting cells, Sorcs3 gene knockout mouse embryonic stem cells are obtained through a gene editing technology, and in-vitro induced trophoblast stem cells (iTSCs) are efficiently and rapidly obtained by means of a flow cytometry sorting technology and in combination with specific antibody sorting. According to the invention, the Sorcs3 gene is knocked out from the embryonic stem cell, so that the capability of the embryonic stem cell to differentiate to the trophoblast lineage can be obviously enhanced; an important research platform is provided for deeply researching a lineage intertransformation mechanism of cell masses (ICM) and trophoblast (TE) in the blastocysts, and the method has important significance for researching lineage limitation of early embryonic development of mammals and the like.

The invention relates to a mouse model construction method for psoriasis research based on dihydroxyisobutyrylation modification of SBSN 600 site lysine. The mouse model construction method comprises the following steps: 1, constructing a plasmid comprising an SBSN gene sequence and a DNA fragment of which the 600 site of the SBSN sequence is mutated from lysine (K) to threonine (T); 2, culturing the mouse embryonic stem cells; step 3, carrying out electric transformation on the embryonic stem cells; 4, screening the medicines; 5, calling and culturing the embryonic stem cells screened in the step 4; step 6, injecting the embryonic stem cells called and cultured in the step 4 into a mouse blastocyst, then culturing the mouse blastocyst, and transplanting the cultured blastocyst into the uterus of a pseudopregnant female mouse, so that the mouse generated by delivery of the female mouse is a chimeric mouse; and 7, breeding the chimeric mouse and identifying the genotype of the mouse through PCR (Polymerase Chain Reaction).

The utility model relates to a thermoplastic polymer pipe variable-diameter thinning mould and equipment capable of ensuring the shape of a conical end part, which comprises a first module and a second module, a first mould cavity of the first module comprises a first equal-diameter pipe cavity, a first conical pipe cavity and a first connecting cavity between the first equal-diameter pipe cavity and the first conical pipe cavity; a second die cavity of the second die set comprises a second equal-diameter pipe cavity, a second taper pipe cavity and a second connecting cavity between the second equal-diameter pipe cavity and the second taper pipe cavity, the inner diameter of the second equal-diameter pipe cavity is larger than the inner diameter of the first equal-diameter pipe cavity and smaller than the outer diameter of the pipe, the die cavity comprises an equal-diameter cavity and a taper pipe cavity, and the taper of the taper pipe cavity is consistent with the taper of the taper end of a finished balloon product. And the shape and the taper of the end part of the blastocyst can be kept while the pipes at the two ends of the blastocyst are thinned. Meanwhile, the conical end part of the balloon embryo can be heated and stretched when being positioned in the conical tube cavity, and after the step, the end part even does not need to be stretched and shaped independently, but only needs to be shaped together with the whole balloon, so that the processing technology is simplified, and the production efficiency is improved.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a glucose slow-release structure used in an embryo in-vitro culture solution, a preparation process and a use method. The glucose slow-release structure comprises at least one inner layer containing glucose and a biodegradable material outer layer wrapping the inner layer, and the biodegradable material outer layer comprises a matrix with a plurality of micropores and at least one sealing material layer used for sealing pores of the micropores. According to the invention, gradient release from low sugar in the cleavage stage to high sugar in the blastocyst stage is realized, midway liquid change or artificial sugar supplement is not needed, the operation is obviously simplified, and pollution and mechanical stress risks are reduced.

The invention discloses a bovine oocyte in-vitro maturation culture solution as well as a preparation method and application thereof, and belongs to the technical field of animal reproduction and embryo engineering. The preparation method of the bovine oocyte in-vitro maturation culture solution comprises the step of adding mitoquinine mesylate into a conventional IVM culture solution, wherein the adding concentration of the mitoquinine mesylate is preferably 50 nmol / L. The bovine oocyte in-vitro maturation culture solution provided by the invention can effectively reduce the oxidative stress reaction of the oocytes in the in-vitro culture process, enhance the mitochondrial function and remarkably improve the bovine oocyte in-vitro maturation quality and the embryonic development potential, so that the blastocyst rate is increased, and the problem that the development potential of the existing oocyte IVM culture solution is insufficient is solved.

The present disclosure relates to methods, products, and compositions useful for deriving bovine induced blastoids. Methods are described that allow for juvenile stem cell aggregation and induced blastocyst formation. The invention further provides an aggregation culture medium and an induced blastocyst-like culture medium which are suitable for promoting aggregation of the young stem cells and preparing the induced blastocyst-like. The bovine induced blastoids can be used in selection and breeding procedures, including the replication of pre-implant embryos with desired characteristics and in vitro breeding including a cross-generation breeding cycle from parent germ cells and / or embryos to progeny embryos.

Described are small molecule cocktails that enable de novo derivation and long-term culture of bovine extraembryonic endoderm cells (bXENs). Methods of using the small molecule cocktails to generate bXENs are also described. Methods of using the bXENs to form blastocyst models or to maintain the stemness of bovine ESCs and prevent them from differentiation are also described.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The invention discloses a method for improving developmental potential of cloned embryos through high expression of Smarca5 and application of the method. According to the method, after the SCNT embryo is reconstructed, the proportion of 4 cell periods, the blastocyst formation rate and the proportion of inner cell clusters are remarkably increased by microinjection of Smarca5mRNA (500ng / mL). Immunofluorescence and qPCR results show that the expression of EGA related genes such as Polr2a and Dppa2 is up-regulated through high expression of the Smarca5, the synthesis level of new RNA is enhanced, and the abundance of H3K9me3 is remarkably reduced by adjusting the balance of Kdm4b / Suv39h2. According to the method, the embryo epigenetic reprogramming level is improved, the SCNT development quality is remarkably improved, and the method can be widely applied to the fields of animal cloning and embryo engineering.

It is revealed that an organ such as pancreas can be regenerated by utilizing a fact that the deficiency of an organ is complemented by injecting an induced pluripotent stem cell (iPS cell) into a developed blastocyst in a blastocyst complementation method. Thus, the present invention has solved the above-described object. This provides a method for producing a target organ, using an iPS cell, in a living body of a non-human mammal having an abnormality associated with a lack of development of the target organ in a development stage, the target organ produced being derived from a different individual mammal that is an individual different from the non-human mammal.

The present invention relates to a medium composition for in vitro fertilization and / or in vitro culture comprising a compound represented by chemical formula 1 or a pharmaceutically acceptable salt thereof, and a method using same. The compound or composition suppresses reactive oxygen species and lipid peroxidation in ovarian granulosa cells to preventing cytotoxicity and mitochondrial dysfunction caused by apoptosis and ferroptosis, leading to an improvement in the quality of aged oocytes, and to increase the developmental rate and blastocyst formation rate of pre-implantation embryos, thereby improving the efficiency of in vitro fertilization. In addition, treatment with the medium composition during the vitrification process of in vitro–fertilized embryos improves re-expansion and survival rates, thereby reducing structural and metabolic damage. Accordingly, the present invention can be advantageously applied to an assisted reproductive technology for treating infertility and subfertility, and in vitro fertilization and / or in vitro culture for improving the propagation efficiency of livestock.

The invention relates to an artificial intelligence prediction method and system for a multi-embryo blastocyst culture outcome. The method comprises the following steps: acquiring morphological parameter data of embryo culture; constructing a feature vector based on the morphological parameter data; and inputting the feature vector into a preset classifier, and predicting a culture result of the embryo group. The method is suitable for various culture environments, and not only can treat group culture, but also can be applied to period prediction of individual culture. The diversity meets the gap among different culture strategies in the prior art; while the dependence on individual labels is reduced, the prediction accuracy of the method is equivalent to that of an existing method based on single embryo probability prediction, and the technical reliability is ensured.

The invention discloses the use of tributyrin as an additive for embryo in vitro development culture medium, and belongs to the technical field of animal reproductive biology. The invention applies tributyrin as an additive to mouse embryo in vitro development culture medium for the first time, and the tributyrin can significantly improve the blastocyst rate, reduce the ROS content in the embryo, improve the mitochondrial membrane potential in the embryo, improve the ATP level in the embryo and the expression of antioxidant genes, improve the DNA methylation and histone modification level in the embryo, and promote the embryo in vitro development; and tributyrin, as a natural antioxidant and epidrug, is safe and has no toxic side effects, is cheap and easy to obtain, does not need to replace the culture medium during the entire development and culture process, and is simple to operate; the tributyrin provides strong support for in vitro embryo engineering technologies such as the efficient development of embryos in human assisted reproductive technology, mammalian fertilized embryos, parthenogenetic embryos and somatic cell cloned embryos, and has good application prospects.

The invention provides an oocyte in-vitro maturation culture solution additive and application thereof. The additive is tert-butylhydroquinone. By adding 100 [mu] g / mL of TBHQ into a basic culture solution, the first polar body discharge rate of oocytes is remarkably increased to 77.29%, the ROS level is reduced, and the blastocyst rate of in-vitro fertilized embryos is increased. The technology is suitable for breed conservation of livestock genetic resources such as sheep and can replace live livestock transportation to reduce the cost.

The invention belongs to the technical field of bioengineering, and particularly relates to application of RPS26 recombinant protein in promoting early embryonic development of animals. Experiments show that after the RPS26 recombinant protein is added in the early-stage in-vitro embryo culture process of the lamb goats, the blastocyst rate of the lamb goats can be increased to 40.0%; meanwhile, the cell proportion of cell mass in the blastocyst can be remarkably increased, the cell proportion of trophoderm is reduced, and the total number of blastocyst cells is not influenced, so that the blastocyst development rate can be remarkably increased and the blastocyst quality can be improved by utilizing the RPS26 recombinant protein.

The embodiment of the invention discloses an in-vitro fertilization embryo culture blastocyst formation rate prediction method and system. The method comprises the following steps: acquiring parameter information of an in-vitro fertilization embryo to be evaluated; the parameter information serves as input and is input into a pre-trained prediction model, a formation rate prediction result of the to-be-evaluated in-vitro fertilized embryo is obtained, and the pre-trained prediction model is obtained through training based on a LightGBM model; and if the formation rate prediction result of the to-be-evaluated in-vitro fertilized embryo is greater than a preset threshold value, using the to-be-evaluated in-vitro fertilized embryo for subsequent transplantation.

The invention discloses a blastocyst transplantation feasibility prediction model construction method, system and device, a medium and a product, and relates to the field of result prediction.The method comprises the steps that a sample data set is obtained, basic information in the sample data set is screened based on minimum absolute contraction and a selection operator method, and risk indexes are obtained; and establishing a blastocyst transplantation feasibility prediction model, evaluating and calibrating the blastocyst transplantation feasibility prediction model by using the verification set, and finally predicting the blastocyst formation condition by using the evaluated and calibrated blastocyst transplantation feasibility prediction model. The method has reliability and repeatability, the feasibility of blastocyst transplantation can be accurately predicted, and then the formation condition of the blastocyst is accurately predicted.

The invention relates to the technical field of biology, and provides a composition, a culture solution, a product and a method for promoting embryo ectogenesis. The composition is prepared from 8-Br-cGMP and glycyrrhizic coumarin. The composition can reduce the lipid peroxidation level of embryos and reduce the toxic effect of lipid peroxidation on the embryos. Meanwhile, fat hydrolysis and oxidation energy supply can be promoted, and sufficient energy guarantee is provided for in-vitro development of early embryos. In addition, the composition can effectively reduce the active oxygen level in the embryo and reduce the damage of oxidative stress to the embryo. By adopting the composition disclosed by the invention, the embryo ectogenesis can be promoted, and the in-vitro blastocyst formation rate can be increased.