Split-range embryo culture solution and preparation method thereof
An embryo culture medium and culture medium technology, applied in the field of cell culture, can solve problems such as harmful decomposition products, and achieve the effects of improving in vitro growth and development, improving quality and improving success rate
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Embodiment 1
[0027] A split-stage embryo culture medium consisting of cleavage fluid and blastocyst fluid. The cleavage fluid is used for fertilized eggs growing to the 8-cell stage, and the blastocyst fluid is used for fertilized eggs growing from the 8-cell stage to fertilization. During the pre-implantation period. Both the cleavage fluid and the blastocyst fluid are composed of basic culture fluid and embryo nutrition factors, and the components of the inorganic salt buffer in the basic culture fluid are the same.
[0028] The formula of the inorganic salt buffer solution is shown in Table 1, and its pH is between 7.3 and 7.5:
[0029] Table 1 The formula of cleavage fluid and blastocyst fluid
[0030]
[0031] Among them, amino acids include essential amino acids and non-essential amino acids, including essential amino acids and non-essential amino acids required by all embryos, and their content corresponds to the needs of embryos. Amino acids were purchased from Life Technologi...
Embodiment 2
[0037] The present embodiment is basically the same as Example 1, and the difference is that the formula of the basic culture solution is as shown in Table 2:
[0038] The formula of table 2 embodiment 2 basic culture fluid
[0039]
Embodiment 3
[0041] This embodiment is basically the same as Embodiment 1, and its difference is that the formula of embryonic culture fluid is as shown in table 3:
[0042] The formula of table 3 embodiment 3 basic culture fluid
[0043]
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