26 results about "Embryo quality" patented technology

The invention discloses a mouse embryo quality and development stage combined prediction system and method based on deep learning, and the system comprises a data preprocessing module which is used for obtaining a time sequence data set, and a feature extraction module which is used for processing the time sequence data set through a pre-trained OfficientNet model, and obtaining the feature vector of an embryo image corresponding to each time point; the time sequence feature fusion module is used for processing the image feature vectors of all embryos arranged according to the time sequence by using a bidirectional long-short-term memory network to obtain hidden state vectors of all embryo image fusion time sequence information; and the multi-task prediction module is used for inputting the hidden state vector into the embryo quality prediction and development stage classification prediction model after joint optimization to obtain a splicing result of embryo quality prediction and development stage classification prediction. According to the method, two prediction tasks of embryo quality and development stage classification can be processed at the same time by utilizing the combination of the OfficientNet model and the bidirectional long-short-term memory network, so that the model processing efficiency is improved.

The present invention addresses the problem of specifically clarifying the effects on a fertilized egg when 5-ALA is administered to a human female suffering from infertility, and constructing a more effective means for treating infertility through the suppression of chromosomal abnormalities. It has been confirmed that, when 5-ALA is ingested in combination with a drug therapy that is generally performed in the treatment of female infertility, the rate of chromosome aneuploidy in a fertilized egg or the like is significantly reduced, and the qualitative improvement of an embryo through improvement of the stability of the chromosomes is expected.

The invention relates to the technical field of embryo quality evaluation, and discloses an embryo development quality evaluation method based on image recognition. The method comprises the following steps: acquiring an embryonic development image sequence, and judging whether the data volume reaches a preset acquisition threshold value; if a threshold value is reached, constructing an embryo feature vector, and processing the embryo feature vector by a multi-dimensional feature dynamic encoder to obtain optimized feature data; a dynamic game-reinforcement learning framework is adopted, and a development quality evaluation strategy is optimized through interaction of an evaluation agent and an embryo population agent; data collaboration is realized by means of a federated learning technology, an abnormal development detection model is trained in a cross-institution mode, embryo abnormal development behaviors are detected based on adversarial training, and an evaluation result is adjusted; and based on a self-adaptive ladder evaluation mechanism, dynamically adjusting an evaluation standard according to embryonic development characteristics and historical records, and calculating a final quality score. The method can comprehensively extract the multi-dimensional dynamic characteristics of the embryo, optimize the evaluation strategy and accurately detect abnormal development.

This invention discloses a method and system for embryo quality detection, relating to the field of embryo detection technology, including the following steps: S1: fertilized embryos are continuously cultured in an in vitro culture system containing specific metabolic marker detection components, and continuous dynamic imaging of the embryos is performed to obtain a complete dynamic developmental image sequence; S2: the dynamic developmental image sequence is segmented, and temporal developmental features are captured, identifying key time points such as pronucleus appearance and disappearance, cleavage, and blastocyst formation, and generating a segmentation mask corresponding to each frame of the dynamic developmental image sequence; this invention achieves full-process time-difference imaging to capture the temporal features of embryonic development, without trauma or interference, avoiding subjective bias in static observation; by segmenting the dynamic developmental image sequence and capturing temporal developmental features, the segmentation accuracy is high and the anti-interference ability is strong.

The invention relates to the technical field of artificial intelligence in assisted reproduction, in particular to an embryo optimization model construction method, device and equipment and a storage medium. Comprising the following steps: acquiring video data of embryonic development from a plurality of fertilized eggs to a blastocyst stage based on a delayed photography embryo culture technology; constructing time sequence image data according to the video data; the time sequence image data comprises multiple frames of continuous images; performing feature extraction on each frame of image of the time sequence image to obtain a high-dimensional feature corresponding to each frame of image; determining a comparison difference feature with the corresponding reference sample feature, performing fusion, and constructing a fusion feature corresponding to each frame of image; constructing a feature sequence of the target embryo development according to the fusion features; and acquiring a trained embryo quality grading model according to a time dependency relationship of the feature sequence for capturing embryo development. The model provided by the invention can optimize the utilization rate of embryos, reduce unnecessary repeated ovulation promoting cycles, and promote the development of reproductive medicine towards the precision direction.

This invention relates to the fields of biomedical engineering and embryo quality assessment, and particularly to a biomarker for assessing the quality of IVM-derived embryos and its application. The biomarker includes time point parameters t2 and t9 of the IVM-derived embryos, and time interval parameters S2, t9–t7, t9–t8, or tSB–t9. A method for assessing the quality of IVM-derived embryos based on the aforementioned biomarker has been established. This method combines non-invasive temporal imaging technology with morphodynamic multi-parameter analysis, providing a reliable tool for assessing the blastocyst formation potential and chromosomal euploidy of IVM-derived embryos in clinical practice, thus potentially expanding the application of IVM technology in assisted reproductive technology.

This invention provides a method for the cryopreservation and reconstitution of porcine embryos. During the in vitro maturation of porcine oocytes, porcine IVF embryo culture, and embryo freezing and thawing processes, the addition of TSA, 5-Aza-dC, Etomoxir, GSK-J4, and NR, either alone or in combination, improves the development and freezing efficiency of porcine IVF embryos. Experiments show that the oocyte maturation rate, embryo cleavage rate, and blastocyst rate of the groups adding TSA, 5-Aza-dC, and Etomoxir to the porcine oocyte in vitro maturation culture medium and porcine IVF embryo culture medium are significantly higher than those of the control group. Furthermore, the hatching rate of porcine IVF embryos after freezing and thawing is significantly higher in the groups adding GSK-J4 and NR to the freezing and thawing solutions than in the control group. This invention lays the foundation for the production of porcine in vitro fertilized embryos and related scientific research, providing an effective, safe, and feasible strategy for improving the production efficiency and quality of porcine in vitro embryos.

The present application relates to the field of biomedical technology, and more particularly to a prediction model for embryo quality outcome of women of childbearing age undergoing assisted reproductive technology; the present application is based on clinical and laboratory data of women of childbearing age undergoing assisted reproductive technology, uses light GBM multi-factor analysis to screen the key factors of clinical core parameters affecting women of childbearing age undergoing assisted reproductive technology, and performs spear correlation analysis with each clinical core parameter to obtain core factors affecting embryo quality outcome of women of childbearing age undergoing assisted reproductive technology, and to construct a model capable of accurately predicting embryo quality outcome, which can accurately predict the positive influencing factors of embryo quality, effectively reduce the psychological burden of a part of ART pregnant patients, so as to make clinical intervention more targeted, more conducive to the rational allocation of clinical resources, and suitable for large-scale popularization and application.

This invention belongs to the field of plant genetic engineering technology, specifically disclosing a lotus embryo-specific promoter pNnCYP80A and its applications. The nucleotide sequence of the promoter is shown in SEQ ID NO.1. The pNnCYP80A promoter provided by this invention has strong activity and can drive the efficient and specific expression of the GUS reporter gene in the immature embryos of transgenic Arabidopsis thaliana. This promoter can drive the efficient and specific expression of exogenous genes in plant embryo organs, and has broad application prospects in the study of plant embryo organ development and the targeted improvement of embryo quality and yield traits through transgenics.

The invention discloses a culture solution for improving in-vitro culture blastocyst rate of bovine oocytes and application of the culture solution, and belongs to the technical field of cell in-vitro culture. According to the culture solution, kaempferol is added into a basic culture solution, and the addition concentration of the kaempferol is 0.01-1 mu M / L; preferably, the addition concentration of the kaempferol is 0.1 mu M / L. By optimizing the concentration of the kaempferol and the synergistic proportion of the kaempferol and basic nutritional ingredients, the action mechanism of the kaempferol for regulating and controlling the mitochondrial function of the oocyte and meiosis spindle assembly is defined, and the direct association of'oxidation resistance-development potential improvement 'is established, so that the blastocyst formation rate and embryo quality of in-vitro culture of the oocyte are remarkably improved, and the development potential of the oocyte is improved. The requirements of the fields of assisted reproduction, embryo engineering and the like on an efficient and stable oocyte in-vitro culture technology are met.

This invention discloses a combination of single vesicle membrane protein markers for assessing embryo implantation potential, its application, and a method for assessing embryo implantation potential, relating to the field of biomedical testing. The marker combination includes the single vesicle membrane proteins ITGA8 and L1CAM. ITGA8 has been found to predict implantation success, while L1CAM can predict implantation failure. The combination of these two markers achieves an AUC value of 0.90, providing molecular markers for accurate assessment of embryo implantation potential. Compared to existing methods that rely solely on morphological scoring, this invention provides molecular-level assessment indicators, significantly improving the accuracy of embryo implantation potential assessment. It can effectively identify low-potential embryos that are morphologically "high-quality" but have "abnormal" protein signals, overcoming the shortcomings of existing technologies that cannot accurately predict the implantation potential of high-quality embryos. Furthermore, this invention only detects EVS in the culture medium, without contacting the embryo itself, making it a safe and non-invasive assessment method for embryo quality.

The application discloses application of sodium crotonate in improving bovine embryo development potential. In the process of culturing bovine SCNT embryos in vitro, adding sodium crotonate (Nacr) with a proper concentration can promote the development of bovine SCNT cells, significantly improve the development rate and blastocyst rate of the SCNT embryos, improve the embryo quality, and further improve the somatic cell nuclear transfer efficiency of pre-implantation embryos. On this basis, the inventors construct a bovine SCNT early embryo in-vitro culture system, optimize the formula of the in-vitro embryo culture solution and the concentration of Nacr, and further establish a method for improving the development potential of early bovine embryos in vitro. The application can improve the development quality of bovine SCNT embryos, provides a research basis for the rapid propagation of high-yield dairy cattle, high-quality beef cattle and even disease-resistant fine breeds, provides a reference for the batch production of high-quality bovine embryos in vitro, and has important practical significance in the field of SCNT embryo in-vitro culture.

The application discloses application of follicular fluid exosome lncLIPE-AS1 in evaluation of embryo quality of patients with polycystic ovary syndrome and belongs to the field of assisted reproductive technology. The application finds that lncLIPE-AS1 is highly expressed in follicular fluid of patients with polycystic ovary syndrome, and finds that lncLIPE-AS1 is correlated with embryo quality and steroid hormone secretion of patients with polycystic ovary syndrome. Therefore, lncLIPE-AS1 can be used as a biomarker for evaluation of embryo development quality in the process of assisted reproduction of patients with polycystic ovary syndrome and prediction of success rate of assisted reproduction, and can also be used for screening of high-quality oocytes of patients with polycystic ovary syndrome and regulation of steroid hormone expression of patients with polycystic ovary syndrome.

The invention provides a method for improving the developmental capacity of a frozen pig IVF (in vitro fertilization) embryo, which comprises the following steps: in the production of a pig in vitro embryo, before the pig embryo obtained by in vitro fertilization is cryopreserved, carrying out combined treatment by using fenofibric acid, sodium nitroprusside, FTY720-P and dopamine, then carrying out vitrification and unfreezing on the embryo, and during the freezing and unfreezing treatment, carrying out freeze-drying on the embryo to obtain the frozen pig IVF embryo. Z-VAD-FMK and TGF-beta are added into an embryo pretreatment solution, a vitrification freezing solution, an unfreezing solution and an embryo culture solution, so that the in-vitro developmental ability of the porcine embryo after vitrification freezing is improved. Experiments show that the embryo survival rate and the hatching rate of a group in which fenofibric acid, sodium nitroprusside, FTY720-P, dopamine, Z-VAD-FMK and TGF-beta are jointly added are obviously higher than those of a group in which fenofibric acid, sodium nitroprusside, FTY720-P, dopamine, Z-VAD-FMK and TGF-beta are added and a control group. The invention provides an effective, safe and feasible strategy for improving the pig in-vitro embryo production efficiency and embryo quality, and has great popularization value.

The invention relates to a microfluidic device, method, and use for the continuous, label-free isolation of motile, morphologically normal spermatozoa from semen samples This invention offers a gentle, reliable, and efficient platform for sperm selection, serving as an alternative to centrifugation-based techniques in ART laboratories. By maintaining the structural and genetic integrity of sperm, it improves fertilization success, embryo quality, and pregnancy outcomes. The platform also reduces variability between technicians and limits exposure to reactive oxygen species (ROS), ensuring consistent clinical performance. Beyond its application in fertility treatments, the system can be used as a diagnostic tool to assess sperm motility disorders, susceptibility to oxidative stress, and DNA fragmentation dynamics under different environmental conditions or drug exposures.

The invention provides a method for improving cow superovulation mother cell quality and application thereof, and aims to improve cow superovulation effect and oocyte quality by improving methionine level of oocyte development microenvironment. The method comprises the following steps: feeding rumen protected methionine to the superovulation dairy cow in vivo, and / or adding methionine into a culture solution when in-vitro maturation culture is carried out on oocytes obtained by superovulation. In the embryo level, the blastocyst forming rate and the embryo quality are remarkably improved, the embryonic development potential is higher, and the quality is better. According to the method, by feeding rumen protected methionine in vivo and / or culturing and adding methionine in vitro, the embryo output effect of cow superovulation is systematically improved, the oocyte quality and the embryo development potential are comprehensively improved from the functional and molecular level, and an innovative solution is provided for efficient embryo production.

The invention discloses a high-quality embryo screening method based on embryo image and free RNA (cfRNA) multi-modal fusion, and belongs to the technical field of assisted reproduction. The method is based on a single embryo culture system and comprises the following steps: collecting a culture solution of a blastocyst-stage embryo and a corresponding embryo image and clinical information, obtaining molecular characteristics of cfRNA in the culture solution by adopting an ultramicro cfRNA sequencing library preparation technology, and extracting embryo morphology and time sequence dynamic characteristics in combination with a deep learning algorithm. And a multi-modal prediction model fusing molecule and image information is constructed, so that non-invasive accurate evaluation of embryo quality, pregnancy rate and live birth rate is realized. According to the method, the problems of high subjectivity, high invasive risk and insufficient prediction accuracy of traditional morphological evaluation are solved, the accuracy of high-quality embryo screening is improved through multi-modal information fusion, a safer and more efficient embryo evaluation scheme is provided for an assisted reproduction technology, and the method has important clinical application value.

The application discloses an embryo quality intelligent evaluation method, comprising the following steps: establishing a pronucleus stage instance segmentation model; establishing a pronucleus stage target tracking model based on DeepSort; establishing a cleavage stage cell detection model; establishing a cleavage stage cell fragmentation degree rating model; establishing a semantic segmentation model based on U-Net; establishing a quality rating model based on Inception V3; and based on the six models established above, establishing an embryo quality intelligent evaluation model based on a graph convolutional neural network, so as to evaluate the embryo quality. The application models different stages in the embryo development process respectively, fuses the embryo characteristics of different stages, trains the graph convolutional neural network, effectively combines the characteristic parameters with physical significance in different periods, establishes the embryo quality intelligent evaluation model, and by using the intelligent evaluation method instead of manual evaluation, the measuring efficiency is greatly improved while the high precision is maintained.

The invention provides a method for improving the production efficiency of bovine in-vitro embryos, and belongs to the technical field of livestock breeding biology. According to the method, carnosine is added in the bovine oocyte in-vitro maturation stage, melatonin is added in the embryo culture stage after in-vitro fertilization, and the embryonic development effect is remarkably improved through the staged synergistic effect. According to the method, the characteristics of energy metabolism regulation and control of carnosine and oxidation resistance of melatonin are fully utilized, the defects of oxidative stress and insufficient energy supply in in-vitro culture are effectively overcome, the blastocyst development rate and the embryo quality are improved, and the method is high in safety, easy and convenient to operate and good in application prospect.

The invention relates to the technical field of assisted reproduction, and provides a method for predicting embryo uploidy through blastocyst cavity fluid metabolite and application of the method. According to the method, the embryonic chromosome uploidy condition is predicted by detecting the content of pyroglutamic acid in the blastocyst cavity liquid, and accurate quantitative analysis of the pyroglutamic acid in a trace amount of the blastocyst cavity liquid can be realized by adopting a nano electrospray ionization-mass spectrometry (nanoESI-MS) detection technology. Researches find that the content of pyroglutamic acid in aneuploid blastocyst cavity fluid is obviously higher than that of the aneuploid blastocyst, and ROC curve analysis shows that pyroglutamic acid has certain diagnostic value. The invention further constructs a prediction model based on the content of pyroglutamic acid, training and verification are carried out through a machine learning method, and the result shows that the model can effectively predict embryo uploidy, a new theoretical basis and an intervention target are provided for embryo quality evaluation in an assisted reproduction technology, the success rate and safety of assisted reproduction can be improved, and the method is suitable for popularization and application. And the risk caused by the aneuploid embryo is reduced.

The application provides an in-vitro embryo culture solution and a preparation method thereof. The IVM solution of the in-vitro embryo culture solution comprises CaCl2, KCl, KH2PO4, glycine, HEPES sodium salt, lactic acid, MgSO4, NaHCO3, myo-inositol, glucose, essential amino acids, non-essential amino acids, retinoic acid, taurine and hormones. When the in-vitro embryo culture solution is used for mammals such as cattle, the IVF embryo production efficiency and embryo quality of the cattle can be improved.

The invention discloses an improved multi-network fusion embryo image classification method, which comprises the following steps: firstly, processing an embryo image data set, then respectively capturing shallow and deep features by adopting two different feature extraction networks, splicing the two features, introducing a compression-excitation module to reinforce important channel features, and finally, carrying out classification on the embryo image data set. And performing classification decision by using a multi-layer perceptron. Aiming at the problem that a single network model is difficult to give consideration to global semantic information and local detail features at the same time, the method aims to combine the advantages of a plurality of networks and realize more accurate and efficient classification judgment of embryo images through multi-scale feature fusion and an attention mechanism, so that intelligent auxiliary support is provided for embryo quality evaluation.

The invention provides a method for obtaining human primitive state pluripotent stem cells from urine and application of the human primitive state pluripotent stem cells, and belongs to the technical field of biological medicine. The method comprises the following steps: reprogramming human urine-derived stem cells into starting state pluripotent stem cells; and culturing the primitive state pluripotent stem cells in a specific primitive state induction culture medium to obtain the human primitive state pluripotent stem cells. According to the present invention, the directional induction from the urine-derived stem cells to the original-state pluripotent stem cells is achieved for the first time, and the obtained cells can reproduce the mitochondrial physiological state and the metabolism characteristics of the human pre-implantation embryo. The mitochondrial preparation extracted from the cells can be used for preparing products for improving the quality of oocytes or early embryos, and has important application prospects in the fields of assisted reproduction and regenerative medicine.

The invention provides a method for improving the transfer developmental capacity of a frozen bovine IVF (in-vitro fertilization) embryo, which is characterized in that in the processes of freezing and unfreezing the bovine IVF embryo, culturing the embryo in a later period and the like, high-concentration sodium pyruvate, insulin, TLR2 (toll-like receptor 2) and a Cytosporone B are independently or jointly added, so that the survival rate and developmental capacity of the frozen embryo, the pregnancy rate after transplantation and the like are improved. Experiments show that the embryo survival rate, the hatching rate and the embryo transfer pregnancy rate of a group which is jointly added with sodium pyruvate, insulin, TLR2 and Nur77 agonist B are obviously higher than those of a double-substance adding group, a single-substance adding group and a control group. The method lays a foundation for production and related scientific research of in-vitro fertilized embryos in the future, provides an effective, safe and feasible strategy for improving in-vitro embryo production efficiency and embryo quality, and has great popularization value.

The invention discloses an embryo quality marker screening method based on metabonomics, an electrochemical sensor for simultaneously detecting three markers and application, and belongs to the technical field of detection. According to the method, three metabolites, namely 1-methylguanidine acid, L-valine and oleic acid, which are closely related to embryo quality are screened through metabonomics analysis of a conditioned medium for culturing human embryos in vitro in the third celestial body in combination with various statistical methods, and reduced graphene oxide is modified on the surfaces of working electrodes 1, 2 and 3 to serve as a modification material; the reduced graphene oxide has good conductivity, can amplify an electrochemical signal of the electrode, and enhances the recognition of the sensor on three embryo quality markers at the same time. An L-valine molecularly imprinted polymer is synthesized on the surface of a working electrode 1 through electropolymerization, a 1-methylguanidine acid molecularly imprinted polymer is synthesized on the surface of a working electrode 2, an oleic acid molecularly imprinted polymer is synthesized on the surface of a working electrode 3, and the molecularly imprinted polymer has the advantages of high sensitivity and strong specificity. The adsorbability of the sensor to an embryo quality marker is greatly improved, and the specificity of the sensor is improved. According to the invention, quantitative detection of single components of the three embryo quality markers can be realized, simultaneous detection of the three embryo quality markers can also be realized, and a new thought is provided for clinical prediction of embryonic development potential.

The invention relates to a microfluidic device, method, and use for the continuous, label-free isolation of motile, morphologically normal spermatozoa from semen samples This invention offers a gentle, reliable, and efficient platform for sperm selection, serving as an alternative to centrifugation-based techniques in ART laboratories. By maintaining the structural and genetic integrity of sperm, it improves fertilization success, embryo quality, and pregnancy outcomes. The platform also reduces variability between technicians and limits exposure to reactive oxygen species (ROS), ensuring consistent clinical performance. Beyond its application in fertility treatments, the system can be used as a diagnostic tool to assess sperm motility disorders, susceptibility to oxidative stress, and DNA fragmentation dynamics under different environmental conditions or drug exposures.