42 results about "Embryo quality" patented technology

The invention discloses an image detection and evaluation method for an unfrozen form of a fertilized egg embryo. The method comprises the following steps: acquiring a time sequence image sequence after the fertilized egg embryo is unfrozen; performing image standardization processing on the time sequence image sequence to generate a standardized embryo image set; extracting multi-dimensional embryo quality parameters from the standardized embryo image set; wherein the multi-dimensional embryo quality parameters comprise a morphological static parameter set, a dynamic change parameter set and a texture feature parameter set; generating a blastocyst formation rate based on the multi-dimensional embryo quality parameters; outputting a third-level embryo quality evaluation result according to a comparison relationship between the blastocyst formation rate and a preset threshold interval; wherein the three-stage embryo quality evaluation result comprises an excellent embryo grade, a qualified embryo grade and an abnormal embryo grade. The method has the following advantages and effects: full-automatic and multi-dimensional quantitative evaluation of the thawing process of the unfrozen embryos is realized, so that the scientificity of embryo screening and the success rate of clinical pregnancy are improved.

The invention discloses a mouse embryo quality and development stage combined prediction system and method based on deep learning, and the system comprises a data preprocessing module which is used for obtaining a time sequence data set, and a feature extraction module which is used for processing the time sequence data set through a pre-trained OfficientNet model, and obtaining the feature vector of an embryo image corresponding to each time point; the time sequence feature fusion module is used for processing the image feature vectors of all embryos arranged according to the time sequence by using a bidirectional long-short-term memory network to obtain hidden state vectors of all embryo image fusion time sequence information; and the multi-task prediction module is used for inputting the hidden state vector into the embryo quality prediction and development stage classification prediction model after joint optimization to obtain a splicing result of embryo quality prediction and development stage classification prediction. According to the method, two prediction tasks of embryo quality and development stage classification can be processed at the same time by utilizing the combination of the OfficientNet model and the bidirectional long-short-term memory network, so that the model processing efficiency is improved.

The invention discloses an auxiliary reproductive semen treatment device matched with different insemination modes, which comprises a box body with an openable box door, a heating treatment table is arranged at the center of the bottom in the box body, and a treatment plate is placed on the heating treatment table; a control box is arranged at the top, a control circuit board is arranged in the control box, a heat dissipation plate and a display screen are arranged, and keys and knobs are arranged on the two sides; the back of the box is provided with a power interface. Through modular design, the device is compatible with semen treatment of various insemination modes such as IVF, ICSI and IUI; the first processing plate (transverse groove method) and the second processing plate (screen method) are used for screening sperms, through natural movement ability, form and thermotaxis guidance, mechanical damage is reduced, sperm motility is improved, sperm DNA fragment rate is reduced, and therefore ovum fertilization rate, embryo quality and pregnancy outcome are expected to be improved.

The present invention addresses the problem of specifically clarifying the effects on a fertilized egg when 5-ALA is administered to a human female suffering from infertility, and constructing a more effective means for treating infertility through the suppression of chromosomal abnormalities. It has been confirmed that, when 5-ALA is ingested in combination with a drug therapy that is generally performed in the treatment of female infertility, the rate of chromosome aneuploidy in a fertilized egg or the like is significantly reduced, and the qualitative improvement of an embryo through improvement of the stability of the chromosomes is expected.

The invention relates to the technical field of embryo quality evaluation, and discloses an embryo development quality evaluation method based on image recognition. The method comprises the following steps: acquiring an embryonic development image sequence, and judging whether the data volume reaches a preset acquisition threshold value; if a threshold value is reached, constructing an embryo feature vector, and processing the embryo feature vector by a multi-dimensional feature dynamic encoder to obtain optimized feature data; a dynamic game-reinforcement learning framework is adopted, and a development quality evaluation strategy is optimized through interaction of an evaluation agent and an embryo population agent; data collaboration is realized by means of a federated learning technology, an abnormal development detection model is trained in a cross-institution mode, embryo abnormal development behaviors are detected based on adversarial training, and an evaluation result is adjusted; and based on a self-adaptive ladder evaluation mechanism, dynamically adjusting an evaluation standard according to embryonic development characteristics and historical records, and calculating a final quality score. The method can comprehensively extract the multi-dimensional dynamic characteristics of the embryo, optimize the evaluation strategy and accurately detect abnormal development.

The invention provides application of L-2-HG in embryo quality evaluation, an embryo quality evaluation method and application thereof, and relates to the technical field of biology. According to the research of the inventor, the metabolite L-2-HG generated by the embryo in the culture medium can be used as an embryo quality evaluation index, and non-invasive, accurate and reliable embryo quality evaluation can be realized according to the change of the L-2-HG in the culture medium in the culture process, so that the efficiency and success rate of embryo screening are improved.

The invention discloses a line establishment method and a culture system of bovine trophoblast stem cells. The stem cell line is obtained by separation and induction from bovine blastocyst. The embryonic stem cell line has stability. The method can be applied to various life science and medical fields such as bovine animal breeding and breeding, gene editing models, animal cloned donor cells, medical musculoskeletal injury experiments, drug development carriers, vaccine production cell banks, embryo quality analysis and the like, and can be applied to large-scale production and application.

This invention discloses a method and system for embryo quality detection, relating to the field of embryo detection technology, including the following steps: S1: fertilized embryos are continuously cultured in an in vitro culture system containing specific metabolic marker detection components, and continuous dynamic imaging of the embryos is performed to obtain a complete dynamic developmental image sequence; S2: the dynamic developmental image sequence is segmented, and temporal developmental features are captured, identifying key time points such as pronucleus appearance and disappearance, cleavage, and blastocyst formation, and generating a segmentation mask corresponding to each frame of the dynamic developmental image sequence; this invention achieves full-process time-difference imaging to capture the temporal features of embryonic development, without trauma or interference, avoiding subjective bias in static observation; by segmenting the dynamic developmental image sequence and capturing temporal developmental features, the segmentation accuracy is high and the anti-interference ability is strong.

This invention discloses a culture medium additive for improving embryonic developmental potential and its application, belonging to the field of in vitro embryo culture technology. This invention is the first to discover that CPI-455, a KDM5 histone demethylase inhibitor, can significantly improve the blastocyst rate, developmental speed, and embryo quality of preimplantation embryos by increasing H3K4me3 levels within a certain concentration range. Simultaneously, the addition of CPI-455 and NMN can substantially increase the rate of high-quality / high-quality blastocysts and embryo quality in in vitro embryo culture. The embryonic developmental potential additive provided by this invention is of great significance for improving in vitro embryo culture efficiency and embryo quality, accelerating the process of breeding superior embryos, and assisting in research on embryo-related mechanisms.

The embodiment of the invention discloses an embryo quality evaluation method. The method comprises the following steps: aiming at a target embryo to be subjected to embryo quality evaluation, acquiring embryo data generated in a culture process of the target embryo, and analyzing the embryo data to obtain an analysis result; for each evaluation script in the plurality of evaluation scripts, the target embryo quality of the target embryo is evaluated according to the analysis result by executing the evaluation scripts, the plurality of evaluation scripts are obtained through pre-coding based on different evaluation systems, and the evaluation systems are systems used for performing embryo quality evaluation; and obtaining the comprehensive embryo quality of the target embryo according to the target embryo quality corresponding to each evaluation system. According to the technical scheme provided by the embodiment of the invention, the efficiency and accuracy of embryo quality evaluation can be improved.

The invention relates to the technical field of artificial intelligence in assisted reproduction, in particular to an embryo optimization model construction method, device and equipment and a storage medium. Comprising the following steps: acquiring video data of embryonic development from a plurality of fertilized eggs to a blastocyst stage based on a delayed photography embryo culture technology; constructing time sequence image data according to the video data; the time sequence image data comprises multiple frames of continuous images; performing feature extraction on each frame of image of the time sequence image to obtain a high-dimensional feature corresponding to each frame of image; determining a comparison difference feature with the corresponding reference sample feature, performing fusion, and constructing a fusion feature corresponding to each frame of image; constructing a feature sequence of the target embryo development according to the fusion features; and acquiring a trained embryo quality grading model according to a time dependency relationship of the feature sequence for capturing embryo development. The model provided by the invention can optimize the utilization rate of embryos, reduce unnecessary repeated ovulation promoting cycles, and promote the development of reproductive medicine towards the precision direction.

The present invention discloses an IVF-ET embryo selection method based on time-difference imaging multi-task learning, belonging to the field of image data processing and assisted reproduction. The method comprises the following steps: first, using a coding module, effectively extracting spatiotemporal features in embryo time-difference imaging sequences through an expanded three-dimensional convolutional network and a bidirectional long short-term memory network; then, within a multi-task learning framework with shared hard parameters, utilizing shared feature representations, and simultaneously predicting multiple key indicators such as a quality grade based on the Istanbul consensus and a developmental grade based on the Gardner grade through multiple task-specific predictors; during the training process, the AdaTask optimization algorithm and the Frank-Wolfe method are used to optimize model parameters, and a virtual predictor mechanism is introduced to enhance the model's versatility; the present invention can more accurately, objectively, and efficiently assess embryo quality and developmental potential, reduce the burden on embryologists, and improve the success rate of IVF and ET, and can be applied to fields such as animal husbandry and endangered species protection.

This invention relates to the fields of biomedical engineering and embryo quality assessment, and particularly to a biomarker for assessing the quality of IVM-derived embryos and its application. The biomarker includes time point parameters t2 and t9 of the IVM-derived embryos, and time interval parameters S2, t9–t7, t9–t8, or tSB–t9. A method for assessing the quality of IVM-derived embryos based on the aforementioned biomarker has been established. This method combines non-invasive temporal imaging technology with morphodynamic multi-parameter analysis, providing a reliable tool for assessing the blastocyst formation potential and chromosomal euploidy of IVM-derived embryos in clinical practice, thus potentially expanding the application of IVM technology in assisted reproductive technology.

This invention provides a method for the cryopreservation and reconstitution of porcine embryos. During the in vitro maturation of porcine oocytes, porcine IVF embryo culture, and embryo freezing and thawing processes, the addition of TSA, 5-Aza-dC, Etomoxir, GSK-J4, and NR, either alone or in combination, improves the development and freezing efficiency of porcine IVF embryos. Experiments show that the oocyte maturation rate, embryo cleavage rate, and blastocyst rate of the groups adding TSA, 5-Aza-dC, and Etomoxir to the porcine oocyte in vitro maturation culture medium and porcine IVF embryo culture medium are significantly higher than those of the control group. Furthermore, the hatching rate of porcine IVF embryos after freezing and thawing is significantly higher in the groups adding GSK-J4 and NR to the freezing and thawing solutions than in the control group. This invention lays the foundation for the production of porcine in vitro fertilized embryos and related scientific research, providing an effective, safe, and feasible strategy for improving the production efficiency and quality of porcine in vitro embryos.

The present invention discloses an avian leukosis antigen detection kit and a preparation method thereof. The kit comprises an anti-avian leukosis virus P27 protein monoclonal antibody and a horseradish peroxidase-labeled sheep anti-P27 polyclonal antibody as a sandwich secondary antibody. The kit is based on a double-antibody sandwich method and has good specificity for A / B, J, and K subtype avian leukosis viruses prevalent in breeder chickens. The minimum detection limit is 1 ng / mL, the kit has strong anti-interference ability, a low false positive rate, and is easy to operate and highly stable. It is suitable for scenarios such as breeder chicken flock purification, SPF embryo quality monitoring, and epidemiological surveys. It effectively solves the problem of expensive imported products and limited domestic promotion, and has significant economic and social benefits.

The present application relates to the field of biomedical technology, and more particularly to a prediction model for embryo quality outcome of women of childbearing age undergoing assisted reproductive technology; the present application is based on clinical and laboratory data of women of childbearing age undergoing assisted reproductive technology, uses light GBM multi-factor analysis to screen the key factors of clinical core parameters affecting women of childbearing age undergoing assisted reproductive technology, and performs spear correlation analysis with each clinical core parameter to obtain core factors affecting embryo quality outcome of women of childbearing age undergoing assisted reproductive technology, and to construct a model capable of accurately predicting embryo quality outcome, which can accurately predict the positive influencing factors of embryo quality, effectively reduce the psychological burden of a part of ART pregnant patients, so as to make clinical intervention more targeted, more conducive to the rational allocation of clinical resources, and suitable for large-scale popularization and application.

This invention belongs to the field of plant genetic engineering technology, specifically disclosing a lotus embryo-specific promoter pNnCYP80A and its applications. The nucleotide sequence of the promoter is shown in SEQ ID NO.1. The pNnCYP80A promoter provided by this invention has strong activity and can drive the efficient and specific expression of the GUS reporter gene in the immature embryos of transgenic Arabidopsis thaliana. This promoter can drive the efficient and specific expression of exogenous genes in plant embryo organs, and has broad application prospects in the study of plant embryo organ development and the targeted improvement of embryo quality and yield traits through transgenics.

The invention discloses a culture solution for improving in-vitro culture blastocyst rate of bovine oocytes and application of the culture solution, and belongs to the technical field of cell in-vitro culture. According to the culture solution, kaempferol is added into a basic culture solution, and the addition concentration of the kaempferol is 0.01-1 mu M / L; preferably, the addition concentration of the kaempferol is 0.1 mu M / L. By optimizing the concentration of the kaempferol and the synergistic proportion of the kaempferol and basic nutritional ingredients, the action mechanism of the kaempferol for regulating and controlling the mitochondrial function of the oocyte and meiosis spindle assembly is defined, and the direct association of'oxidation resistance-development potential improvement 'is established, so that the blastocyst formation rate and embryo quality of in-vitro culture of the oocyte are remarkably improved, and the development potential of the oocyte is improved. The requirements of the fields of assisted reproduction, embryo engineering and the like on an efficient and stable oocyte in-vitro culture technology are met.

This invention discloses a combination of single vesicle membrane protein markers for assessing embryo implantation potential, its application, and a method for assessing embryo implantation potential, relating to the field of biomedical testing. The marker combination includes the single vesicle membrane proteins ITGA8 and L1CAM. ITGA8 has been found to predict implantation success, while L1CAM can predict implantation failure. The combination of these two markers achieves an AUC value of 0.90, providing molecular markers for accurate assessment of embryo implantation potential. Compared to existing methods that rely solely on morphological scoring, this invention provides molecular-level assessment indicators, significantly improving the accuracy of embryo implantation potential assessment. It can effectively identify low-potential embryos that are morphologically "high-quality" but have "abnormal" protein signals, overcoming the shortcomings of existing technologies that cannot accurately predict the implantation potential of high-quality embryos. Furthermore, this invention only detects EVS in the culture medium, without contacting the embryo itself, making it a safe and non-invasive assessment method for embryo quality.

The invention discloses application of IL17D in rescuing oocytes damaged by cold stress, a culture solution capable of rescuing oocytes damaged by cold stress, application of the culture solution in rescuing oocytes damaged by cold stress, application of the culture solution in improving quality of somatic cell cloned embryos, and application of the culture solution in rescuing oocytes damaged by cold stress. The invention further discloses a method for improving the in-vitro maturation quality of the oocytes damaged by cold stress. The invention also discloses an application of IL17D in reducing the active oxygen level in the oocytes damaged by cold stress, an application in improving the glutathione content in the oocytes damaged by cold stress and an application in improving the antioxidant gene expression in the oocytes damaged by cold stress. The IL17D is added into the in-vitro maturation culture solution of the oocytes, so that the damage of cold stress to the porcine oocytes can be saved, and a new solution and a new idea can be provided for solving the problems that the in-vitro maturation quality of the oocytes is influenced and the embryo quality is further influenced due to the fact that the oocytes are damaged by the cold stress in winter.

The invention provides application of a cumulus cell mTOR-S6 protein marker in non-invasive prediction of embryo quality and pregnancy outcome, and relates to the technical field of bioengineering. According to the present invention, the Westernblot technology is adopted to quantitatively detect the levels of the mTOR signal channel key proteins such as p-mTOR, mTOR, p-S6 and S6 in cumulus cells; meanwhile, the system collects IVF related clinical indexes of the patient, correlation analysis is carried out on the clinical indexes and the relative levels of the four key proteins through a statistical method, and it is found that the IVF related indexes of the patient are evaluated with the median value of the relative expression values of the four proteins as a threshold value. Whether the ovarian reserve function, the embryonic development quality and the pregnancy outcome are good or not can be judged. The invention not only provides an innovative auxiliary evaluation tool for the field of reproductive medicine, but also provides a scientific basis for formulating an individualized IVF treatment scheme.

Differentially expressed miRNA or small mRNA in the culture media of embryos were found to correlate to and affect embryo developmental fate. Accordingly the present invention provides a method for selecting a bovine embryo for implantation into a female bovine animal for further development based on the levels of specific miRNA or small mRNA. Also provided are methods of improving bovine embryo development fate by reducing in the culture medium the level of selected miRNAs.

The application discloses application of sodium crotonate in improving bovine embryo development potential. In the process of culturing bovine SCNT embryos in vitro, adding sodium crotonate (Nacr) with a proper concentration can promote the development of bovine SCNT cells, significantly improve the development rate and blastocyst rate of the SCNT embryos, improve the embryo quality, and further improve the somatic cell nuclear transfer efficiency of pre-implantation embryos. On this basis, the inventors construct a bovine SCNT early embryo in-vitro culture system, optimize the formula of the in-vitro embryo culture solution and the concentration of Nacr, and further establish a method for improving the development potential of early bovine embryos in vitro. The application can improve the development quality of bovine SCNT embryos, provides a research basis for the rapid propagation of high-yield dairy cattle, high-quality beef cattle and even disease-resistant fine breeds, provides a reference for the batch production of high-quality bovine embryos in vitro, and has important practical significance in the field of SCNT embryo in-vitro culture.

The application discloses application of follicular fluid exosome lncLIPE-AS1 in evaluation of embryo quality of patients with polycystic ovary syndrome and belongs to the field of assisted reproductive technology. The application finds that lncLIPE-AS1 is highly expressed in follicular fluid of patients with polycystic ovary syndrome, and finds that lncLIPE-AS1 is correlated with embryo quality and steroid hormone secretion of patients with polycystic ovary syndrome. Therefore, lncLIPE-AS1 can be used as a biomarker for evaluation of embryo development quality in the process of assisted reproduction of patients with polycystic ovary syndrome and prediction of success rate of assisted reproduction, and can also be used for screening of high-quality oocytes of patients with polycystic ovary syndrome and regulation of steroid hormone expression of patients with polycystic ovary syndrome.

The invention provides a method for improving the developmental capacity of a frozen pig IVF (in vitro fertilization) embryo, which comprises the following steps: in the production of a pig in vitro embryo, before the pig embryo obtained by in vitro fertilization is cryopreserved, carrying out combined treatment by using fenofibric acid, sodium nitroprusside, FTY720-P and dopamine, then carrying out vitrification and unfreezing on the embryo, and during the freezing and unfreezing treatment, carrying out freeze-drying on the embryo to obtain the frozen pig IVF embryo. Z-VAD-FMK and TGF-beta are added into an embryo pretreatment solution, a vitrification freezing solution, an unfreezing solution and an embryo culture solution, so that the in-vitro developmental ability of the porcine embryo after vitrification freezing is improved. Experiments show that the embryo survival rate and the hatching rate of a group in which fenofibric acid, sodium nitroprusside, FTY720-P, dopamine, Z-VAD-FMK and TGF-beta are jointly added are obviously higher than those of a group in which fenofibric acid, sodium nitroprusside, FTY720-P, dopamine, Z-VAD-FMK and TGF-beta are added and a control group. The invention provides an effective, safe and feasible strategy for improving the pig in-vitro embryo production efficiency and embryo quality, and has great popularization value.

The invention relates to a microfluidic device, method, and use for the continuous, label-free isolation of motile, morphologically normal spermatozoa from semen samples This invention offers a gentle, reliable, and efficient platform for sperm selection, serving as an alternative to centrifugation-based techniques in ART laboratories. By maintaining the structural and genetic integrity of sperm, it improves fertilization success, embryo quality, and pregnancy outcomes. The platform also reduces variability between technicians and limits exposure to reactive oxygen species (ROS), ensuring consistent clinical performance. Beyond its application in fertility treatments, the system can be used as a diagnostic tool to assess sperm motility disorders, susceptibility to oxidative stress, and DNA fragmentation dynamics under different environmental conditions or drug exposures.

The invention provides a method for improving cow superovulation mother cell quality and application thereof, and aims to improve cow superovulation effect and oocyte quality by improving methionine level of oocyte development microenvironment. The method comprises the following steps: feeding rumen protected methionine to the superovulation dairy cow in vivo, and / or adding methionine into a culture solution when in-vitro maturation culture is carried out on oocytes obtained by superovulation. In the embryo level, the blastocyst forming rate and the embryo quality are remarkably improved, the embryonic development potential is higher, and the quality is better. According to the method, by feeding rumen protected methionine in vivo and / or culturing and adding methionine in vitro, the embryo output effect of cow superovulation is systematically improved, the oocyte quality and the embryo development potential are comprehensively improved from the functional and molecular level, and an innovative solution is provided for efficient embryo production.

The invention discloses a high-quality embryo screening method based on embryo image and free RNA (cfRNA) multi-modal fusion, and belongs to the technical field of assisted reproduction. The method is based on a single embryo culture system and comprises the following steps: collecting a culture solution of a blastocyst-stage embryo and a corresponding embryo image and clinical information, obtaining molecular characteristics of cfRNA in the culture solution by adopting an ultramicro cfRNA sequencing library preparation technology, and extracting embryo morphology and time sequence dynamic characteristics in combination with a deep learning algorithm. And a multi-modal prediction model fusing molecule and image information is constructed, so that non-invasive accurate evaluation of embryo quality, pregnancy rate and live birth rate is realized. According to the method, the problems of high subjectivity, high invasive risk and insufficient prediction accuracy of traditional morphological evaluation are solved, the accuracy of high-quality embryo screening is improved through multi-modal information fusion, a safer and more efficient embryo evaluation scheme is provided for an assisted reproduction technology, and the method has important clinical application value.

The application discloses an embryo quality intelligent evaluation method, comprising the following steps: establishing a pronucleus stage instance segmentation model; establishing a pronucleus stage target tracking model based on DeepSort; establishing a cleavage stage cell detection model; establishing a cleavage stage cell fragmentation degree rating model; establishing a semantic segmentation model based on U-Net; establishing a quality rating model based on Inception V3; and based on the six models established above, establishing an embryo quality intelligent evaluation model based on a graph convolutional neural network, so as to evaluate the embryo quality. The application models different stages in the embryo development process respectively, fuses the embryo characteristics of different stages, trains the graph convolutional neural network, effectively combines the characteristic parameters with physical significance in different periods, establishes the embryo quality intelligent evaluation model, and by using the intelligent evaluation method instead of manual evaluation, the measuring efficiency is greatly improved while the high precision is maintained.

The invention provides a method for improving the production efficiency of bovine in-vitro embryos, and belongs to the technical field of livestock breeding biology. According to the method, carnosine is added in the bovine oocyte in-vitro maturation stage, melatonin is added in the embryo culture stage after in-vitro fertilization, and the embryonic development effect is remarkably improved through the staged synergistic effect. According to the method, the characteristics of energy metabolism regulation and control of carnosine and oxidation resistance of melatonin are fully utilized, the defects of oxidative stress and insufficient energy supply in in-vitro culture are effectively overcome, the blastocyst development rate and the embryo quality are improved, and the method is high in safety, easy and convenient to operate and good in application prospect.