The invention discloses a
screening method of different metabolites for evaluating goat transgenic cloned
embryo quality based on a
gas chromatography-
mass spectrum
combined technique and morphology. The
screening method comprises the following steps that 1, reconstructed embryos are cultured for 48 hours, the cleavage rate is checked, cleaved embryos are divided into a high-quality cleaved
embryo group and a low-quality cleaved
embryo group according to the morphology, the embryo groups are randomly put into
blastocyst culture droplets for continuous culture; 2, the
blastocyst rate statistics is performed at 7.5 d, and blastocysts and embryo metabolites of the high-quality cleaved embryo group and the low-quality cleaved embryo group are respectively collected; 3, the collected embryo
metabolite samples are processed, and
gas chromatography-
mass spectrum detection is performed; 4, detection results are subjected to statistic analysis. The
screening method is integrated with morphological evaluation of the embryos to find
metabolism markers closely correlated with the goat transgenic cloned
embryo quality, provides a reference for research on prediction of embryonic developmental potential and facilitates screening of high-quality transplanted embryos and improvement of transgenic
cloning efficiency.