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69 results about "Maturation oocyte" patented technology
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System for in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
InactiveUS7094527B2Promote divisionQuality improvementAnimal reproductionDead animal preservationX chromosomeBiology
An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comrprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY
In-Vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
ActiveUS20060281176A1Promote divisionQuality improvementAnimal reproductionDead animal preservationX chromosomeBiology
An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY
Culture solution capable of improving in vitro maturation rate of pig oocytes and application thereof
InactiveCN107099500AMaturation Efficiency ImprovementA large amountCulture processGerm cellsPenicillinBiology
The invention belongs to the technical field of pig genetic breeding, and specifically relates to a culture solution capable of improving in vitro maturation rate of pig oocytes and application thereof. The culture solution capable of improving the in vitro maturation rate of pig oocytes is prepared from the following raw materials with the following concentrations: 9.5 g / L of TCM-199, 2.2 mg / mL of NaHCO3, 3.05 mmol / L of D-glucose, 0.91 mmol / L of Sodium Pyruvate, 0.5 g / L of Streptomycin sulfate, 0.05% of PVA, 0.57 mmol / L of Cysteine and 0.075 g / L of Penicillin G. The 0.05% of PVA and 5% of a follicular fluid are added on the basis of pig oocyte in vitro maturation culture solution widely used in our country, so that the in vitro maturation rate of the pig oocytes can be improved to more than 90%, and matured oocytes with higher quality and more quantity can be provided for porcine somatic cell nuclear transfer technology.
Owner:GUANGXI UNIV
Method for promoting in-vitro maturing of human immature oocyte by utilizing 3D printing technology
The invention discloses a method for promoting in-vitro maturing of human immature oocyte by utilizing a 3D printing technology. The method comprises the following steps: separating and collecting granular cells in clinic, propagating the granular cells in an in-vitro culture mode for 2 to 7 days; evenly mixing biological hydrogel with the pre-processed granular cells, printing artificial follicles by a biological 3D printing technology; adding a culture liquid into the artificial follicles, wherein the volume percentage content of human mature follicular fluid of the culture liquid is not less than 10%; culturing for 24 to 48 hours, then adding an immature oocyte-cumulus granular cell composite body, and culturing the 3D cells for 24 to 48 hours to promote the maturing. In the provided method, a 3D cell printing technology is adopted, human granular cells are used to construct an immature oocyte 3D culturing system, and multiple growth factors and simulated follicle culture liquid are added at the same time to create a simulated human follicle so as to promote the in-vitro maturing of immature oocyte.
Owner:ZHEJIANG UNIV +1
New purpose of traditional Chinese medicine compound medicine composition
ActiveCN102784267APromote maturityPromote growth and developmentUnknown materialsGerm cellsSemenCytoskeleton
The invention provides a purpose for preparing supplementary reproduction medicine from the following raw material medicine in percentage by weight: 18 to 30 parts of radix rehmanniae preparata, 9 to 12 parts of dogwood, 8 to 16 parts of Chinese yam, 9 to 12 parts of fructus lycii, 8 to 16 parts of semen cuscutae and 8 to 16 parts of antler gum. The invention also provides a method for promoting ovarian ovum in vitro maturation. The method comprises the following steps that a, an immature ovarian ovum is taken; and b, the immature ovarian ovum is placed in M199 culture liquid, a microdrop method is adopted for culture for 24 hours, and a mature ovarian ovum is obtained, wherein 2 percent of medicine is contained in the culture liquid. The medicine can promote the growth and development of the immature ovarian ovum in vitro and mainly promotes the ovarian ovum core maturation, and in addition, the cytoskeleton abnormality probability of the mature ovarian ovum is not increased. The medicine can also generate the influence on the ovum passing capability of sperms, and the reliable basis is provided for clinically preventing and treating the male sperm dysfunction, improving the IVF (in vitro fertilization) success ratio and reducing the use rate of ICSI (intracytoplasmic sperm injection).
Owner:CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
In-vitro cultivating matural process of immatural ovocyte in ovarium organized block
The extracorporeal culture and mature method of immature oocyte from ovary tissue block includes selecting marrow mesenchyme stem cell MSC of female mouse as the feeder cell, compounding MSC culture liquid and agar and covering the culture rack, adding HCG in 3 IU / ml, and adhering the ovary tissue block on the rack to culture. In the co-culture system, estradiol, progestin and luteotopin contents are increased obviously, and after culturing, there is mature oocyte discharged. The method is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.
Owner:ANHUI PROVINCIAL HOSPITAL
Cloning using rapidly matured oocytes
InactiveUS20030217378A1Improve efficiencyImprove usabilityMicroinjection basedFermentationProphase IMammal
A method of producing a cloned or genetically modified non-human mammalian embryo comprising: (a) providing a cell culture comprising a plurality of in vitro matured oocytes; (b) preferentially selecting from the cell culture a rapidly matured oocyte or developmentally competent oocyte; (c) transferring DNA from a donor cell derived from non-human mammalian tissue to the matured oocyte to form a nuclear transfer unit; and (d) culturing said nuclear transfer unit to form an embryo. At the initiation of maturation, oocytes are preferably beyond the GV-II stage of prophase I. Porcine oocytes most preferably mature in about 20-28 hours.
Owner:UNIV OF GEORGIA RES FOUND INC
Immature oocyte in-vitro maturation culture solution and application thereof
ActiveCN107475181AImprove maturity rateIncreased potential for later developmentNew breed animal cellsCulture processMaturation oocyteCulture fluid
The invention relates to cell culture and particularly discloses an immature oocyte culture scheme which can promoting in-vitro maturation of immature oocytes. Specially, the scheme adopts follicular granulosa cells and a culture solution for culturing the follicular granulosa cells, wherein the culture solution for culturing the follicular granulosa cells contains CNP or its variants or analogues and an HDAC (histone deacetylase) inhibitor. The invention further provides an in-vitro maturation culture solution containing the CNP or its variants or analogues and the HDAC inhibitor and related compositions and application of a culture medium, the culture solution and the compositions in promotion of in-vitro maturation of immature oocytes.
Owner:CHINA AGRI UNIV
Mature optimized human oocyte cytoplasm fluid
ActiveCN107384854AImproves cytoplasmic maturityIncreased rate of high-quality embryosNew breed animal cellsCulture processMaturation oocyteCulture fluid
The invention discloses mature optimized human oocyte cytoplasm fluid. The invention develops the mature optimized human oocyte cytoplasm fluid by adding some important hormones, energy substances and efficient antioxidant melatonin on the basis of low-sugar TCM199 tissue culture solution and patient autoserum. The invention develops a culture solution which is suitable for mature optimization of the oocyte cytoplasm in IVF-ET period and also is suitable for reutilization after in vitro maturity of the immature oocyte in the period. The mature optimized human oocyte cytoplasm fluid is capable of improving the maturity degree of the cytoplasm of the matured oocyte. A clinical result proves that the high-quality embryo rate of the oocyte is greatly increased, the development potential of the immature oocyte is increased and some immature oocytes in the IVF-ET period have a reutilization value.
Owner:江苏瑞辅达医疗器械有限公司
Simple in-vitro maturation culture method for oocytes
InactiveCN102154198AReduce dependenceLowering the Barriers to ResearchGerm cellsMaturation oocyteWater baths
The invention discloses a simple in-vitro maturation culture method for oocytes, which comprises the following steps: A1, collecting oocytes; A2, preparing a closed air bag and performing in-vitro maturation culture of oocytes; and A3, performing parthenogenetic activation and in-vivo culture of oocytes, namely after 22 to 24 hours of in-vitro maturation culture, selecting mature oocytes discharging first polar bodies, activating by a chemical process in the 28th hour, culturing for 3 days in merogenesis culture solution, culturing for 4 days in blastaea culture solution, and observing and counting a blastaea rate after 7 to 9 days. When a self-prepared closed air bag system is used, the in-vitro culture of the oocytes and embryos can be accomplished by using a common biochemical culture tank or water bath pot, so the dependency on expensive instruments is reduced and a research doorsill is reduced. When the simple closed air bag is used for in-vitro culture of the oocytes, blastaea can be grown, and the in-vitro maturation rate, merogenesis rate and blastaea rate are higher than those of oocytes cultured in a normal CO2 culture tank.
Owner:NORTHWEST A & F UNIV
New application of traditional Chinese medicine compound medicinal composition
ActiveCN103479690APromote maturityPromote growth and developmentUnknown materialsSexual disorderSemenCytoskeleton
The invention provides a purpose for preparing supplementary reproduction medicine from the following raw material medicine in percentage by weight: 18 to 30 parts of radix rehmanniae preparata, 9 to 12 parts of dogwood, 8 to 16 parts of Chinese yam, 9 to 12 parts of fructus lycii, 8 to 16 parts of semen cuscutae and 8 to 16 parts of antler gum. The invention also provides a method for promoting ovarian ovum in vitro maturation. The method comprises the following steps that a, an immature ovarian ovum is taken; and b, the immature ovarian ovum is placed in M199 culture liquid, a microdrop method is adopted for culture for 24 hours, and a mature ovarian ovum is obtained, wherein 2 percent of medicine is contained in the culture liquid. The medicine can promote the growth and development of the immature ovarian ovum in vitro and mainly promotes the ovarian ovum core maturation, and in addition, the cytoskeleton abnormality probability of the mature ovarian ovum is not increased. The medicine can also generate the influence on the ovum passing capability of sperms, and the reliable basis is provided for clinically preventing and treating the male sperm dysfunction, improving the IVF (in vitro fertilization) success ratio and reducing the use rate of ICSI (intracytoplasmic sperm injection).
Owner:CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
In-vitro cultivating matural process of immatural ovocyte through promoting discharging separating or sucting obtained in ovarium organized
The method of obtaining immature oocyte from ovary tissue through separating or extracting for extracorporeal culture includes selecting marrow mesenchyme stem cell MSC of female mouse as the feeder cell or culturing the immature oocyte in the MSC culture liquid. After culturing, the co-culture system has obviously increased estradiol, progestin and luteotopin contents, and over 90 % of the immature oocyte become mature. The method is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.
Owner:ANHUI PROVINCIAL HOSPITAL
In-vitro culture solution of immature oocytes, and preparation method and application of in-vitro culture solution
ActiveCN111518749ASingle ingredientClear efficacyCulture processCell culture active agentsAnimal scienceCorpus luteum graviditatis
The present invention relates to the technical field of assisted reproduction and discloses an in-vitro culture solution of immature oocytes. The in-vitro culture solution comprises a basic culture solution and a composition. The basic culture solution comprises a solvent and a solute, the solvent comprises water, and the solute comprises vitamins, energy substances, inorganic salts and amino acids; and the composition comprises 0.01-1 mg / L of butyrolactone-I, 0.001-2 mg / L of estradiol, 0.001-1 IU / L of follicle-stimulating hormone, 0.001-1 IU / L of luteinizing hormone, 0.001-1 IU / L of epidermalgrowth factor, 0.001-1 mg / L of insulin-like growth factor and 0.001-1 IU / L of human chorionic gonadotropin. The present invention also discloses a preparation method and an application of the in-vitro culture solution. In addition to adding basic substances, by adding the energy substances, composition and vitamins, nucleus and cytoplasm of the immature oocytes are synchronously mature and besides, a fertilization rate and a high-quality embryo rate are improved.
Owner:成都艾伟孚生物科技有限公司
Mullerian inhibiting substance levels and ovarian response
Methods and kits are provided for assessing the ovarian reserve and predicting the ovarian response to fertility treatments in a female subject. The serum levels of MIS are shown to be positively correlated with the production and retrieval of mature oocytes and serve as prognostic indicators for the female response to fertility treatment. The MIS levels can be monitored prior to and during fertility treatment and are useful to adjust the timing and dosage of treatments in order to produce optimal outcome in individual patients, to avoid ovarian hyperstimulation, or to indicate cancellation of an unsuccessful treatment. MIS can also be administered to women to stimulate follicle development and to prevent depletion of ovarian reserve.
Owner:RUTGERS THE STATE UNIV
Method for improving transferring efficiencies of pig somatic cell nucleuses
ActiveCN102943093AImprove the blastocyst rateQuality improvementFermentationGenetic engineeringEmbryoPerivitelline space
The invention discloses a method for improving transferring efficiencies of pig somatic cell nucleuses. The method includes the following steps of selecting and cultivating egg mother cells; separating and cultivating donor cells; using a blind aspiration method to remove a mature egg mother cell nucleus and a first polar body, injecting a donor cell to perivitelline space, subjecting a reconstructed embryo to electrofusion, and activating the reconstructed embryo; and placing the reconstructed embryo in a PZM-3 culture solution containing RG108 with concentration of 100-200 mu M and Scriptaid with concentration of 100-500 nM to be cultivated for 14-16 hours, and then placing the reconstructed embryo in a PZM-3 culture solution for cultivation. According to the method, development blastosphere rates and blastosphere qualities of pig cloning early embryos can be obviously improved; and simultaneously, two epigenetic modification of gene methylation and acetylation with close connection and synergistic effects can be adjusted, the method is better in effects than that only by using the Scriptaid for treatment, early genetic expression of embryos can be adjusted to be normal, and finally the transferring efficiencies of the pig somatic cell nucleuses are improved.
Owner:广东中芯种业科技有限公司
Specimen culturing assembly suitable for use in in-vitro fertilization and in other cell culturing procedures
ActiveUS7985579B2Bioreactor/fermenter combinationsBiological substance pretreatmentsViewing instrumentEmbryo
Owner:COOPERSURGICAL INC +1
Feeder cells using in immatural ovocyte in-vitro cultivating matural
InactiveCN100999724AInhibition of apoptosisSynthesis and secretion promotionSkeletal/connective tissue cellsOvarian tissueCytokine
The present invention is feeder cell for extracorporeal culture of immature oocyte and features that marrow mesenchyme stem cell MSC of female mouse is used as the feeder cell. The feeder cell is easy to obtain, can secrete several kinds of cell factors and growth factors, promote over 90 % of the immature oocyte to become mature and promote the growth, ovulation and maturing of the ovarian follicles in the ovary tissue block under extracorporeal culture. The present invention is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.
Owner:ANHUI PROVINCIAL HOSPITAL
Immature oocyte in vitro maturation promoting culture medium and application thereof
ActiveCN107460161AImprove the ability of in vitro culture and developmentIncrease available quantityCulture processGerm cellsMaturation oocyteCulture fluid
The invention relates to an immature oocyte in vitro maturation promoting culture medium and application thereof. The immature oocyte in vitro maturation promoting culture medium is screened out through research on effects of different additives on immature oocytes and prepared by adding 20-100 mu M of BAPTA-AM in normal oocyte in vitro maturation culture solution. The immature oocyte in vitro maturation promoting culture medium is safe and free to side and toxic effects, enables meiosis of the immature oocytes, particularly those in level 3 cumulus-oocyte complexes, improves the in vitro development of the oocytes, promotes maturation, improves the cleavage rate and the blastula development rate after the mature oocytes are parthenogenetically-activated and further improves embryo quality. Application of the immature oocyte in vitro maturation promoting culture medium provides favorable support for the technology of in vitro propagation of animal embryos.
Owner:CHINA AGRI UNIV
Vitrification refrigerating liquid and refrigerating method thereof
InactiveCN108244100AImprove survival rateIncrease cleavage rateDead animal preservationVitrificationMaturation oocyte
The invention belongs to the field of assisted reproduction and particularly relates to vitrification refrigerating liquid. According to the vitrification refrigerating liquid provided by the invention, taxol is added to serve as a cryoprotectant and the vitrification refrigerating liquid obtained with reference to the taxol adding mode is treated, so that the development rate of in vitro fertilized blastocyst after the oocyte is thawed can be increased. The taxol serving as an additive in the refrigerating liquid can achieve the effects of reducing freezing injury of the mature oocytes and improving the development capability of the embryo after fertilization. The optimal concentration of the adding mode is obtained by researching the adding concentration of the taxol. The vitrification refrigerating liquid provided by the invention can greatly increase recovery rate after the frozen mature oocytes are thawed and the development rate of the blastula and achieves the protective effecton the cell ultrastructure.
Owner:成都艾伟孚生物科技有限公司
Application of enhancing antioxidation of oocyte cultured in vitro by Peroxiredoxin 4 protein
PendingCN109517788ADelay agingImprove fertilization rateCell culture active agentsGerm cellsMaturation oocyteImmature oocyte
The invention provides an application of enhancing antioxidation of an oocyte cultured in vitro by a Peroxiredoxin 4 protein. The maturing rate of the immature oocyte cultured in vitro can be improvedby improving the resistance of particle cells in oxidative stress and improving the antioxidation of the oocyte. Aging of the oocyte is also delayed through an antioxidant stress mechanism in the cell, and the fertility rate and the clinical pregnancy rate of the oocyte in an assisted breeding process are improved.
Owner:JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
Culture solution for promoting in-vitro maturation of porcine oocytes
ActiveCN110951678APromote maturityQuality improvementCulture processCell culture active agentsPhysiologyNuclear transfer
The invention discloses a culture solution for promoting in-vitro maturation of porcine oocyte, the culture solution takes an M199 culture solution without HEPES as a basic culture solution, and porcine follicular fluid, L-cysteine, sodium pyruvate, epidermal growth factor, insulin, gonadotropin, chorionic gonadotropin and WNT5A cytokine are also added. When the culture solution is used for carrying out in-vitro culture on the porcine oocytes, the in-vitro maturation efficiency and quality of the oocytes can be effectively improved, the obtained mature oocytes are used for carrying out porcinein-vitro fertilization and somatic cell nuclear transfer embryo production, and the blastocyst development rate and quality of the mature oocytes are also remarkably improved.
Owner:HUAZHONG AGRI UNIV
Method for improving parthenogenetic development potential of vitrified mature oocytes
InactiveCN109294978AProlong the action timeROS content decreasedCell culture active agentsEmbryonic cellsVitrificationMaturation oocyte
The invention discloses a method for improving the parthenogenetic development potential of vitrified mature oocytes, wherein oocytes are frozen in a melatonin-containing freezing liquid, and then arethawed with a melatonin-containing sucrose solution, the thawed oocytes are cultured, the cultured oocytes are subjected to parthenogenetic activation, and finally the parthenogenetically activated embryo is cultured in vitro. According to the present invention, the method has advantages of simple process and short time consumption, and can effectively improve the parthenogenetic activation development potential of vitrified mature oocytes.
Owner:SICHUAN AGRI UNIV
Extraction method and application of mouse ovarian tissue fluid
InactiveCN102174467AIncrease cleavage rateAnimal reproductionGerm cellsMaturation oocyteEmbryo transfer
The invention provides an extraction method of a mouse ovarian tissue fluid. The method comprises the following steps: placing mouse ovaries in a basal medium; mashing and dissloving the ovarian tissue fluid in the basal medium to form a pre-culture solution. The invention also provides an in-vitro maturation culture method of mouse oocyte, and the method comprises: transferring an immature mouse oocyte into a mature culture solution for in-vitro culture, wherein the mature culture solution is formed by adding the pre-culture solution in the basal medium of oocytes. In addition, the invention also provides a method for obtaining a test-tube mouse through embryo transplantation, and the method comprises: carrying out in-vitro fertilization and embryo transplantation on the oocyte matured by the in-vitro maturation culture method. By using the ovarian tissue fluid provided by the invention in the in-vitro maturation of the mouse oocyte, the cleavage rate of the in-vitro fertilized mature egg can be effectively increased, and is nearly the same as the that of the in-vivo mature egg; and finally, the test-tube mouse can be obtained through embryo transplantation.
Owner:上海斯莱克实验动物有限责任公司
Method for evaluating influences of smoking on quality of oocytes
The invention discloses a method for evaluating the influences of smoking on the quality of oocytes. Specifically, oocytes are cultured to be mature in culture solutions containing different concentrations of cotinine. Finally, the influences of smoking on the quality of oocytes are evaluated by detecting some indicators, such as the maturation rate of oocytes, chromosomal aneuploidy, actin distribution, mitochondrial membrane potential, intracellular reactive oxygen species level, spindle morphology, chromosome arrangement of mature oocytes, sperm binding number after in-vitro fertilization,pronucleus formation rate after parthenogenetic activation and the like. Through a large number of objective tests, the influences of smoking on the quality of oocytes are evaluated, thus providing areference of theoretical data for the maintenance of reproductive capacity of female smokers.
Owner:NANTONG UNIVERSITY
Human oocyte in vitro maturation culture solution containing Endothelin-1 and application of human oocyte in vitro maturation culture solution
ActiveCN105018418AImprove developmental potentialImprove fertilization rateGerm cellsMaturation oocyteAnimal science
The invention provides a human oocyte in vitro maturation culture solution containing Endothelin-1. The human oocyte in vitro maturation culture solution comprises liquid A and liquid B, wherein the liquid A comprises ET-1, r-FSH and 10% SSS, the liquid B comprises ET-1, r-FSH, hCG and 10% SSS, and solvent is conventional basic culture solutions. Researches show that the culture solution and culture method are capable of promoting human oocyte in vitro maturation especially cytoplasmic maturation and can increase embryo development potential after human oocyte in vitro fertilization. The culture solution aiming at the current situation that development imbalance of cytoplast and cell nucleus during immature human oocyte in vitro culture affects embryo in vitro development potential is developed by utilizing the biological function and effect of ET-1 in cumulus cells. The culture solution has the advantages that the culture solution is applicable to human oocyte in vitro culture, an IVM process can be optimized favorably, and the culture solution is worthy of popularization and application.
Owner:ZHEJIANG UNIV
Artificial reproduction-hybridization joint breeding method for meat goat
InactiveCN111406711AIncrease the number ofImprove survival rateCulture processGerm cellsSemenZoology
The invention belongs to the field of animal breeding and particularly relates to an artificial reproduction-hybridization joint breeding method for a meat goat. The method comprises the steps of selecting one goat variety with good meat performance as a female parent, selecting another goat variety with a large body size as a male parent; culturing oocytes of the female parent in vitro into mature oocytes containing a first polar body, and soaking semen of the male parent with a semen motility enhancement liquid and then preparing a semen suspension; carrying out in vitro fertilization on themature oocytes and the semen suspension, culturing zygotes into a hybrid blastocyst form, transplanting hybrid blastocysts into a body of the female parent for developing to natural delivery in the body of the female parent and obtaining hybrid F1; and backcrossing the hybrid F1 and the female parent, backcrossing backcross descendants and the female parent for multiple times until a breeding objective is met, and carrying out crossbred fixing to obtain a new variety / line with good characters. According to the method, an artificial assistant reproduction technology is combined with a traditional hybridization technology, so that the number of animal embryos can be increased, the survival rate of the animal embryos can be improved and the method has a good application prospect in the fieldof animal breeding.
Owner:SHANXI AGRI UNIV
Transgenic animals
Owner:WISCONSIN ALUMNI RES FOUND
Method for lamb oocyte in-vitro fertilization culture
The invention belongs to the field of biology, and particularly relates to a method for lamb oocyte in-vitro fertilization culture. The method comprises the steps of oocyte acquisition and maturation culture, sperm capacitation, mature oocyte in-vitro fertilization and zygote culture. During sperm capacitation and mature oocyte in-vitro fertilization, incubating time, the rotating speed of a centrifugal machine, centrifugation time, the number of times of centrifugal washing and mature oocyte culture time conditions are optimized, so that the zygote cleavage rate of lambs is basically the same as that of adult ewes.
Owner:YANGZHOU UNIV
Vitro maturation of immature human oocytes
The invention provides methods for in vitro maturation of immature human oocytes. In one aspect, the invention provides a method for in vitro maturation of immature human oocytes, comprising the steps of: (a) inducing in a female human subject an increase in endogenous luteinizing hormone levels, said subject having not undergone an ovarian stimulation protocol prior to said inducing step; (b) obtaining from said subject an immature oocyte; and (c) culturing said oocyte until maturity.
Owner:MCGILL UNIV
Human Oocyte Cytoplasmic Maturation Optimization Solution
ActiveCN107384854BImproves cytoplasmic maturityIncreased rate of high-quality embryosNew breed animal cellsCulture processBiotechnologyAntioxidant
The invention discloses mature optimized human oocyte cytoplasm fluid. The invention develops the mature optimized human oocyte cytoplasm fluid by adding some important hormones, energy substances and efficient antioxidant melatonin on the basis of low-sugar TCM199 tissue culture solution and patient autoserum. The invention develops a culture solution which is suitable for mature optimization of the oocyte cytoplasm in IVF-ET period and also is suitable for reutilization after in vitro maturity of the immature oocyte in the period. The mature optimized human oocyte cytoplasm fluid is capable of improving the maturity degree of the cytoplasm of the matured oocyte. A clinical result proves that the high-quality embryo rate of the oocyte is greatly increased, the development potential of the immature oocyte is increased and some immature oocytes in the IVF-ET period have a reutilization value.
Owner:江苏瑞辅达医疗器械有限公司
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