Immature oocyte in vitro maturation promoting culture medium and application thereof

A technology for in vitro maturation of oocytes, applied in the field of reproductive biology, can solve the problems of in vitro maturation and culture of uncertain third-level cumulus-oocyte complexes, etc., so as to improve potential utilization value, improve embryo quality, and improve embryo quality. quality effect

Active Publication Date: 2017-12-12
CHINA AGRI UNIV
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still uncertain whether BAPTA-AM can promote the in vitro maturation of the tertiary...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The immature oocyte maturation culture in vitro of embodiment 1 bovine

[0033] (1) Collection of oocytes

[0034] Collect the ovaries of dairy cows from the slaughterhouse, put them in a thermos flask containing double-antibody (0.024g / L penicillin + 0.02g / L streptomycin), keep the temperature at 35°C-37°C, and send them within 6 hours Back to the lab. The ovaries were washed 2 to 3 times with normal saline containing double antibodies, and then washed with 2ml egg extraction solution (9.5g / L M199+10%FBS+0.042%NaHCO3+0.2383%Hepes+0.2603%Hepes-Na+0.001%heparin sodium + 0.005% penicillin + 0.0065% streptomycin) into a 10 ml syringe with a 18-gauge needle to extract the oocyte-follicular fluid complex of 3-8 mm follicles. Put the oocyte extraction liquid after absorbing the oocytes into a domestic petri dish with a diameter of 9 cm, and select first-class COCs (cumulus-oocytes with uniform cytoplasm and 5 or more layers of surrounding granulosa cells) under a stereo mic...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an immature oocyte in vitro maturation promoting culture medium and application thereof. The immature oocyte in vitro maturation promoting culture medium is screened out through research on effects of different additives on immature oocytes and prepared by adding 20-100 mu M of BAPTA-AM in normal oocyte in vitro maturation culture solution. The immature oocyte in vitro maturation promoting culture medium is safe and free to side and toxic effects, enables meiosis of the immature oocytes, particularly those in level 3 cumulus-oocyte complexes, improves the in vitro development of the oocytes, promotes maturation, improves the cleavage rate and the blastula development rate after the mature oocytes are parthenogenetically-activated and further improves embryo quality. Application of the immature oocyte in vitro maturation promoting culture medium provides favorable support for the technology of in vitro propagation of animal embryos.

Description

technical field [0001] The invention relates to a culture medium for promoting in vitro maturation of immature oocytes and application thereof, belonging to the technical field of reproductive biology. Background technique [0002] In terms of reproductive biology, embryo production requires a large number of egg sources. Generally, oocytes obtained directly from the ovary of slaughtered animals are used, or oocytes are obtained from the ovaries of living animals by live egg collection. However, due to the The obtained oocytes are not fully mature, so a period of in vitro maturation and culture is required. After the immature oocytes are matured, the subsequent stages of in vitro fertilization and development into embryos can be completed, and reproduction and production can be truly realized. [0003] Mammalian ovaries contain a large number of immature oocytes. Therefore, taking immature oocytes directly from the ovary for in vitro maturation and culture is the most effect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2500/36
Inventor 侯云鹏胡洪梅傅祥伟莫显红
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap