A culture medium for promoting immature oocyte maturation in vitro and its application

An oocyte, in vitro maturation technology, applied in the field of reproductive biology, can solve the problems of in vitro maturation and cultivation of uncertain tertiary cumulus-oocyte complexes, etc. The effect of improving embryo quality
CN107460161BActive Publication Date: 2020-07-21CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2020-07-21
Patent Text Reader

Abstract

The invention relates to an immature oocyte in vitro maturation promoting culture medium and application thereof. The immature oocyte in vitro maturation promoting culture medium is screened out through research on effects of different additives on immature oocytes and prepared by adding 20-100 mu M of BAPTA-AM in normal oocyte in vitro maturation culture solution. The immature oocyte in vitro maturation promoting culture medium is safe and free to side and toxic effects, enables meiosis of the immature oocytes, particularly those in level 3 cumulus-oocyte complexes, improves the in vitro development of the oocytes, promotes maturation, improves the cleavage rate and the blastula development rate after the mature oocytes are parthenogenetically-activated and further improves embryo quality. Application of the immature oocyte in vitro maturation promoting culture medium provides favorable support for the technology of in vitro propagation of animal embryos.
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Description

technical field

[0001] The invention relates to a culture medium for promoting in vitro maturation of immature oocytes and application thereof, belonging to the technical field of reproductive biology. Background technique

[0002] In terms of reproductive biology, embryo production requires a large number of egg sources. Generally, oocytes obtained directly from the ovary of slaughtered animals are used, or oocytes are obtained from the ovaries of living animals by live egg collection. However, due to the The obtained oocytes are not fully mature, so a period of in vitro maturation and culture is required. After the immature oocytes are matured, the subsequent stages of in vitro fertilization and development into embryos can be completed, and reproduction and production can be truly realized.

[0003] Mammalian ovaries contain a large number of immature oocytes. Therefore, taking immature oocytes directly from the ovary for in vitro maturation and culture is the most effect...

Claims

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