33 results about "BAPTA" patented technology

The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of zinc ions in a sample. The metal chelating moiety of the zinc-binding compound is an analog of the well-known calcium chelator, BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′N′-tetraacetic acid), wherein the chelating moiety has been modified from a tetraacetic acid moiety to a tri- di- or monoacetic moiety. This change in acetic acid groups on the metal chelating moiety results in the selective bindings of zinc ions in the presence of calcium ions, both of which are present in biological fluids and intracellular cytosolic fluid and organelles.

An improved method of preserving a molecule in a bodily fluid comprises: (1) providing a preservative solution comprising: (a) an amount of a divalent metal chelator selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), (ethylenebis(oxyethylenenitrilo))tetraacetic acid (EGTA), and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) and salts thereof in the range of from about 0.001 M to about 2 M; and (b) an amount of at least one chelator enhancing component selected from the group consisting of lithium chloride, guanidinium chloride, guanidinium thiocyanate, sodium salicylate, sodium perchlorate, and sodium thiocyanate in the range of from about 0.1 M to about 10 M; and (2) adding the preservative solution to the bodily fluid, thus preserving the molecule. The molecule can be a protein or a small molecule, such as a steroid. The invention also encompasses preservative compositions suitable for preserving proteins or small molecules, and kits. Preservative compositions can further include at least one enzyme inactivating component selected from the group consisting of manganese chloride, sarkosyl, and sodium dodecyl sulfate in the range of up to about 5% molar concentration. Compositions and methods according to the present invention have many diagnostic and forensic uses, in addition to being suitable for preparing compositions usable by hunters for attracting animals.

A new class of visible wavelength fluorescent calcium indicators with the BAPTA-like portion rendered zwitterionic by introduction of amine and carboxylic groups. “Fluo” compounds generally function with no extra ionized groups. The modified BAPTA moiety confers new properties while retaining ion selectivity and pH insensitivity. The dyes demonstrate reduced cell leakage and improved ability to study calcium near the cell membrane and preserve the fluorescent properties of “fluo” dyes. The modifications include (a) piperazinoacetic acid XIII for the leakage resistance (FLUO-LR), (b) dodecylpiperazine XIII for a near membrane indicator (FLUO-MOMO, FLUO-NOMO amphipathic “fluo” indicators which bind to cellular membranes and respond to calcium near the membrane), and (c) a propionic acid XII for general leakage resistance -FLUO-LOJO, FLUO-KOJO “fluo” indicators with an extra charge that enables them to resist leakage).

The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a —N,N,O-triacetic acid analog of BAPTA and has the following formula

A method of reducing the damaging effect of an injury to mammalian cells by treatment of the cell or mammalian tissue in vivo with a cell membrane permeant calcium buffer. The method comprises treating mammalian tissue with a damage reducing effective amount of the calcium buffer, preferably, a BAPTA derivative. The method may be used to control the concentration of Ca2+ ions in the vicinity of ion channel pores of the cells to prevent diffusion of toxic amounts of Ca2+ ions to subcellular sites located near the source of Ca2+ influx. The buffer treatment may be applied as a prophylactic or after the mammalian tissue has sustained injury.

The invention discloses an application of a medicament in magnesite flotation and calcium removal, and belongs to the technical field of magnesite processing and purifying. An inhibitor BAPTA is applied to a magnesite flotation and calcium removal technology. The application method comprises following steps: grinding high calcium low-grade magnesite, carrying out ball milling to obtain magnesite powder; adding magnesite powder into flotation equipment, adding deionized water and an inhibitor namely a BAPTA dimethyl sulfoxide solution, evenly mixing, blending, adding NaOH into the magnesite slurry at a room temperature to adjust the pH to 10-12, adding a collecting agent (sodium oleate), evenly mixing, and carrying out direct floatation and rougher flotation to obtain low calcium magnesiteconcentrate. Due to the floatation difference of added inhibitor BAPTA on magnesite and impurity ore (dolomite) in magnesite, the calcium ores in magnesite can be removed, the magnesite quality is improved, and a novel medicament is provided for the flotation and calcium removal of high calcium low-grade magnesite.

In order to keep the precious marine products alive, the coelom cell apoptosis rate of the stichopus japonicus can be regulated and controlled when the stichopus japonicus is stimulated by the outside, and then autolyzing of the body wall of the stichopus japonicus can be delayed. The invention provides a method for delaying autolyzing of body wall of stichopus japonicas. A calcium ion chelating agent BAPTA-Na is injected into the coelom of the stichopus japonicus; the calcium ion chelating agent BAPTA-Na. The calcium ion chelating agent BAPTA-Na is injected into the coelom of the stichopus japonicus in different doses in a median lethal concentration range, and with the increase of the dosage, the coelom cell apoptosis of the stichopus japonicas is obviously inhibited, autolyzing of the body wall is delayed, and the purpose of preserving and keeping the stichopus japonicus is achieved. The BAPTA-Na is directly injected into the coelom of the living stichopus japonicus and can directlyact with calcium ions in a coelom cell, and the living experiment verifies that the new application of BAPTA-Na has persuasion.

The present invention relates to the use of lipophilic diesters of the chelating agent 1,2-bis(2 aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) for inhibition of proteolytic activities of certain metalloproteinases and of calpain. The invention further relates to methods for preventing, treating or managing MMP-related and calpain-related diseases or disorders in mammals comprising administering to a mammal in need thereof, a pharmaceutical composition comprising a therapeutically effective amount of said lipophilic diesters of the chelating agent BAPTA.

The invention relates to the field of pharmaceutical chemistry, and particularly relates to an improved BAPTA derivative general formula (I), a preparation method of the improved BAPTA derivative, a medicinal preparation containing the improved BAPTA derivative, and medicinal use of the improved BAPTA derivative, wherein R, R1, R2 and n are defined as in the description. The invention further provides a preparation method of the derivative compounds and applications in medicine.

The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of zinc ions in a sample. The metal chelating moiety of the zinc-binding compound is an analog of the well-known calcium chelator, BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid), wherein the chelating moiety has been modified from a tetraacetic acid moiety to a tri- di- or monoacetic moiety. This change in acetic acid groups on the metal chelating moiety results in the selective bindings of zinc ions in the presence of calcium ions, both of which are present in biological fluids and intracellular cytosolic fluid and organelles.

The invention relates to design and synthesis of subcellular area targeting, long-wavelength and dual-wavelength ratio method Ca<2+> fluorescent probe. In the synthesis of the probes, 1, 2-di(2-aminophenoxy)ethane-N, N, N', N'-tetraacetic acid tetraethyl ester is taken as the initial material to first synthesize Ca<2+> receptor (5, 5'-dicarboxo-BAPTA) with two formyl active groups as the key intermediate of the Ca<2+> fluorescent probes. Then, the key intermediate is condensed with the substrate containing active methyl in agreeable organic solvent to obtain a series of Ca<2+> fluorescent probes excited by visible light, fluorescence excitation and emission peak as well as position all change before and after the probes combine with Ca<2+>, and Ca<2+> can be measured by dual-wavelength ratio method. The probes can carry out targeting marking on the specified subcellular area after entering cells. With the probes adopted, the real-time dynamic measurement of Ca<2+> content in the cells and space-time distribution condition can be realized in the subcellular level, thereby, an efficient means for cell calcium signal transduction research is provided.

The invention provides a novel calcium-ion selective chelating agent as shown in the formula (I) and a preparation method and application thereof. The compound has excellent capacity of ischemia and oxygen deprivation resistance, meanwhile has excellent transcellular capacity serving as a transport substrate of OATP1B1 transport proteins and has very important meaning and potential value in research of BAPTA derivates.

The invention discloses a nontoxic environmental-protection building decorative material which is composed of the following ingredients, by weight, 2-3 parts of maleic anhydride, 5-10 parts of a film-forming additive, 1-3 parts of microlite, 3-4 parts of a phosphite antioxidant, 10 parts of a carboxylic styrene butadiene emulsion, 2-5 parts of open-cell expanded perlite, 4-8 parts of zinc oxide, 1-3 parts of silicon oxide, 8-11 parts of polypropylene, 5-10 parts of BAPTA, 10-14 parts of natural latex, 10-20 parts of a fire retardant, 3-6 parts of an anti-aging agent, 1-3 parts of glycerol triglycerate and 5-20 parts of a cosolvent. The invention has the following beneficial effect: microlite powder, an organic filler and the like are used as main raw materials, and the prepared building decorative material contains no chemical toxic or harmful substances, is environmentally-friendly and is simple to operate and convenient to construct.