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A kind of method for delaying autolysis of sea cucumber body wall

An autolysis and ginseng technology, applied in the application, fish farming, animal husbandry and other directions, can solve the problems of cumbersome operation, short survival time, high cost, and achieve the effect of inhibiting cell apoptosis and delaying body wall autolysis.

Active Publication Date: 2021-09-24
DALIAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method has a higher cost, a shorter preservation time, and is not suitable for long-distance transportation; there is also an anesthesia preservation, using MS-222, 3-aminobenzoic acid ethyl ester methanesulfonate, but its solution is acidic, and MS-222 cannot reduce CO when seafood is anesthetized and kept alive 2 Discharge, it must be raised in clean water for 21 days before it can be sold, and its operation is relatively cumbersome
At present, it is difficult to keep the sea cucumber fresh and alive in seafood, because it is stimulated by the outside world, and it will autolyze

Method used

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  • A kind of method for delaying autolysis of sea cucumber body wall
  • A kind of method for delaying autolysis of sea cucumber body wall
  • A kind of method for delaying autolysis of sea cucumber body wall

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Embodiment approach

[0018] BAPTA-Na semi-lethal concentration detection: Take the body cavity fluid near 1 / 3 of the anus of sea cucumber, mix equal volumes of the body cavity fluid of 9 sea cucumbers, filter with 300-600 mesh filter cloth, take 100 μL of fresh body cavity fluid in 96 In the well plate; add 0.00-2.00mM calcium ion chelating agent BAPTA-Na with different gradients; add 10 μL MTT solution to each well, and incubate at room temperature for 3-5 hours; add 100 μL Formanzan solution to each well, and incubate at room temperature in the dark 3 to 5 hours; use a multi-functional microplate reader to measure the absorbance at 570nm; calculate the IC based on the cell viability 50 It is 1.47mM, so select high, medium and low doses below 1.47mM to pour into the body cavity of sea cucumber.

[0019] Experimental grouping: Fresh and alive sea cucumbers were randomly divided into four groups, with 9 sea cucumbers in each group. UVA group Apostichopus was treated by ultraviolet light (UVA, 10~2...

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Abstract

In order to keep sea treasures fresh and alive, regulate the apoptosis rate of body coelom cells of sea cucumbers when they are stimulated by the outside world, and delay the autolysis of sea cucumber body walls, the present invention provides a method for delaying body wall autolysis of sea cucumbers, Inject the calcium ion chelating agent BAPTA-Na into the body cavity of the sea cucumber; the calcium ion chelating agent BAPTA-Na. The calcium ion chelating agent BAPTA-Na adopted in the present invention is poured into the body cavity of sea cucumbers in different doses within the semi-lethal concentration range. Dissolve, in order to achieve the purpose of keeping fresh and alive sea cucumber. BAPTA-Na is directly infused into the body cavity of fresh sea cucumber, which directly interacts with calcium ions in coelomocytes, and the in vivo experiment verifies that the new application of BAPTA-Na is convincing.

Description

technical field [0001] The invention relates to the technical field of marine organisms, and more specifically relates to a method for inhibiting coelomocyte apoptosis of sea cucumbers and delaying body wall autolysis of sea cucumbers by applying calcium ion chelating agent BAPTA-Na. Background technique [0002] Calcium ion chelating agent BAPTA-Na chemical name is 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid disodium salt, molecular formula is C 22 h 22 N 2 Na 2 o 10 , with a molecular weight of 520.40, is a commercially synthesized calcium ion chelating agent. According to the existing literature search, BAPTA has the following effects: 1. It can weaken the neurotransmitter central mammalian synapse release and reduce neuron ischemia in vivo; 2. It can significantly block the EGF-induced MCF-7 breast cancer cells Migration; 3. BAPTA pretreatment of pulmonary microvascular endothelial cells can promote the recovery of PMVECs mitochondrial permeability tran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/30
CPCA01K61/30
Inventor 宋亮周大勇张晶丁月田景玉刘自强刘玉欣朱蓓薇
Owner DALIAN POLYTECHNIC UNIVERSITY
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