37results about How to "Reduce autolysis" patented technology

The invention relates to a black tea fungus growing process, and in particular relates to a technology for making selenium-rich black tea. The technology comprises the following steps: carrying out pile fermentation on raw black tea material, steaming the tea, spraying eurotium cristatum conidial powder onto the tea, sealing the tea and then sterilizing, carrying out fungus growing process on the tea, carrying out ultrasonic treatment, etc. By adopting the ultrasonic treatment, the method can reduce the autolysis phenomenon of eurotium cristatum, and also has the function of fixing golden flowers, thus being beneficial to protecting organic selenium to the utmost extent, reducing the high-temperature oxidative degradation of the organic selenium, and preventing the thallus from being reduced by the golden flowers under the high-temperature condition. A great deal of eurotium cristatum conidial powder is adopted for quick fermentation, so that the growth of sundry fungus can be reduced, the problem of tea mildew can be avoided, and the influence of a culture medium in the eurotium cristatum liquid to the original flavor of the tea can be avoided.

The invention provides a method for preparing a non-fried instant freshwater fish, comprising the concrete steps of preparing a raw material fish, carrying out pretreatment (scale, head, tail and innards removing), washing clearly, cutting and shaping, soaking in ice water, soaking in water containing salt and vinegar, curing at a low temperature, draining, performing roast smoking, stoving, baking, spouting oil, filling, carrying out vacuum seal and microwave sterilization, boxing up to obtain the finished product. The fish prepared by adopting the method for preparing the non-fried instant freshwater fish has the advantages of no fishy smell, good taste, no poisonous or harmful substance of fried foods, little oil content and capability of guaranteeing the product quality.

A method is provided for producing medium chain length poly(3-hydroxyalkanoate) (MCL-PHA) with a selected ratio of monomers. A method of controlling the ratio of monomers in MCL-PHA is also provided which includes fermenting naturally occurring microorganisms with a fatty acid substrate, a food source and an additive. The methods provided do not sacrifice cell growth and maintenance and provide high yields of MCL-PHAs. MCL-PHAs are provided that are copolymers of Cn and Cn-2 monomers, where (n is 6-18).

The invention aims to disclose a tea processing method to solve problems that ampelopsis grossedentata tea has poor taste, poor color and luster degree and too slow dissolution speed of effective components, and ensure that tea leaf fungus growing has good uniformity, and the tea is convenient for drinking, can keep the original quality of traditional fermented tea and is rich in organic selenium and flavonoids substances. The processing method successively comprises the following processing steps: preparing fresh ampelopsis grossedentata raw materials, carrying out fixation, carrying out pile fermentation, adding sodium selenite, steaming the tea, spraying eurotium cristatum spore powder, sterilizing after sealing tea leaves, carrying out tea leaf fungus growing, carrying out ultrasonic treatment, and baking and packaging. A large amount of eurotium cristatum spore powder is employed for rapid fermentation, thus miscellaneous bacteria growth is reduced, the tea leaf moldy problem cannot be caused, and the effect of a eurotium cristatum liquid culture medium on the original flavor of the tea leaves is avoided; with addition of the ampelopsis grossedentata leaves, the growth of harmful bacteria can be significantly inhibited, and the content of selenium in the tea leaves can be increased; and the ultrasonic treatment can reduce autolysis phenomenon of eurotium cristatum, can achieve the function of fixing eurotium cristatum, is conducive to furthest protect organic selenium, reduces high-temperature oxidation degradation of organic selenium, and simultaneously also can prevent eurotium cristatum from shrinking thalli at the high temperature.

The invention discloses a method for preparing a bovine cortical bone grafting material, comprising the following steps: degreasing and decalcifying a cleaned bovine cortical bone block, immersing the bovine cortical bone block in an inhibitor of a bone morphogenetic protein degrading enzyme, then performing deproteinization, and finally obtaining the bovine cortical bone grafting material by freezing and drying. The preparation method has the advantages of simple steps, wide material source, low cost, low antigenicity of the obtained bone grafting material, strong bone formation inducibility, high biomechanical strength.

The invention relates to a health-care tea making process, in particular to a process method for making health-care tea by fermenting sweet tea leaves. The making process comprises the following steps: taking sweet tea raw materials, pile fermentation, tea steaming, eurotium cristatum spore powder spraying, sterilizing after tea leaves are sealed, tea leaves fungus growing, ultrasonic processing, drying and packing and the like. The sweet tea making process has the ultrasonic treatment capacity, can reduce the autolysis phenomenon of eurotium cristatum, also can achieve the function of fixing golden flowers, is beneficial to protection of organic selenium to the greatest degree, reduces the high-temperature oxidative degradation of organic selenium, and meanwhile, and can also prevent the thalli of the golden flowers from being reduced under the high temperature condition, the quick fermentation is carried out by adopting a large amount of eurotium cristatum spore powder, the growth of infectious microbes is slowed down, the problem of mildewing of the sweet tea leaves is not caused, the influence of a culture medium in the eurotium cristatum solution on the original flavor of the tea leaves is avoided, the content of tea polyphenol is reduced through fermentation, and the stomach irritation influence is reduced, and the sweet tea is easily suitable for being drunk by people with diabetes by means of the sweet taste of rubusoside.

The invention discloses an application of longan fresh-keeping paper. The longan fresh-keeping paper is formed through sequentially packaging a base material, a coating and loading layer, an SO2 releasing agent and a packaging material, the base material and the packaging material are made of breathable and permeable materials, and the coating and loading layer is selected from an acrylate pressure-sensitive adhesive, a non-setting adhesive and a 3M adhesive; and the SO2 releasing agent is formed through mixing a main release agent with a release accelerant according to a mass ratio of 1:0.10-0.25. When the longan fresh-keeping paper is used, fruits are covered with the fresh-keeping paper, water vapor goes through the packaging material at the top of the fresh-keeping paper and enters the fresh-keeping paper in the fruit fresh-keeping process, the releasing agent absorbs water and then releases SO2, and the density of SO2 is greater than the density of air, so released SO2 sinks, uniformly diffuses in a fruit pile from top to bottom, and is uniformly absorbed by the fruits; and when the fresh-keeping paper is used to carry out fresh keeping, the good fruit rate of fruits stored at 1-5DEG C for 30d is 90-100%, and the shelf life in a 15-25DEG C air conditioning room is 3-5d.

The invention relates to a method for reducing free ammonia in anchovy protein hydrolysate. Currently, a method for effectively removing free ammonia is not presented in the production process of enzymolysis of seawater anchovy protein. The method comprises the following steps: firstly, regulating the temperature of hydrolysate to 25-35 DEG C; then adding active dried yeast in a proportion that 0.01-0.1g of active dried yeast is added in per L of hydrolysate, or adding yeast culture in a proportion that 10-100 ml of yeast culture is added in per L of hydrolysate; and after evenly stirring, standing for 1-4 hours at the temperature of 25-35 DEG C, heating to 55-70 DEG C, insulating for 2-6 hours; and then raising temperature to 80-90 DEG C and maintaining for 10-20 minutes to inactivate the yeast. The method in the invention has the effect of obviously reducing free ammonia in protein hydrolysate, and can be used for effectively controlling the pH value of a product, effectively releasing the endogenesis soluble protein of the yeast and increasing the flavor of the product. The method provided by the invention is simple and practicable, and has good effect.

The invention discloses a healthcare composite tea making technology. The technology comprises the following steps: deactivating enzymes of a fresh vine tea raw material, carrying out file fermentation, steaming, sprinkling with Eurotium cristatum spore powder, sealing tea leaves, sterilizing, fermenting the tea leaves, carrying out ultrasonic treatment, drying, and packaging. The technology effectively solves the problems of bad mouthfeel and color, too slow dissolve-out speed of active components of the vine tea, realizes ensure of the good tea leaf fermentation uniformity, convenient drinking, and maintenance of the original quality of a traditional fermented tea, and allows a tea rich in organic selenium and flavonoid substances to be obtained.

The invention relates to a thawing agent for euphausia superba and an application method of the thawing agent, which belong to the technical field of food processing. The thawing agent is mainly prepared from an autolytic enzyme inhibitor and an antioxidant, and can also be prepared from an antibacterial agent. When in use, the thawing agent is dissolved in a solvent to form thawing liquid, and the frozen euphausia sperba is thawed by utilizing the thawing liquid, so that the solution and oxidation of the euphausia superba in the thawing process can be reduced, the shape and quality of the euphausia superb can be kept, the transfer of fluorine can be prevented, and the important significance for the development of the euphausia superb processing industry is realized.

In order to keep the precious marine products alive, the coelom cell apoptosis rate of the stichopus japonicus can be regulated and controlled when the stichopus japonicus is stimulated by the outside, and then autolyzing of the body wall of the stichopus japonicus can be delayed. The invention provides a method for delaying autolyzing of body wall of stichopus japonicas. A calcium ion chelating agent BAPTA-Na is injected into the coelom of the stichopus japonicus; the calcium ion chelating agent BAPTA-Na. The calcium ion chelating agent BAPTA-Na is injected into the coelom of the stichopus japonicus in different doses in a median lethal concentration range, and with the increase of the dosage, the coelom cell apoptosis of the stichopus japonicas is obviously inhibited, autolyzing of the body wall is delayed, and the purpose of preserving and keeping the stichopus japonicus is achieved. The BAPTA-Na is directly injected into the coelom of the living stichopus japonicus and can directlyact with calcium ions in a coelom cell, and the living experiment verifies that the new application of BAPTA-Na has persuasion.

The invention provides an aspergillus niger strain for protein producing and application thereof, which belong to the field of gene engineering. After a fluG gene is knocked out of the aspergillus niger strain, an obtained mutant strain has a protein high-yielding property; further, a saccharifying enzyme regulating factor is introduced into a mutant strain genome, and an obtained mutant strain FY17(delta fluGamyR<+>) can promote secretion of homologous proteins and heterologous proteins; after a pepA gene is knocked out of the FY17 mutant strain, an obtained mutant strain can also be beneficial for remarkably improving secretory volume of the homologous proteins and the heterologous proteins; the invention also discovers that the FY17 SP(delta fluGdeltapepAamyR<+>AnhapC<+>) can be used as an expression vector for producing a plurality of heterologous proteins. The mutant strains adopting the aspergillus niger strain without the fluG gene as an original strain have favorable capabilities on producing the homologous proteins and the heterologous proteins, so that the aspergillus niger strain has a favorable market application prospect.

The invention discloses an article for use in the field of biomedicine, particularly a biological specimen preserving bottle. According to the biological specimen preserving bottle, a preserving bottle is arranged in a protection shell of the preserving bottle, a preserving bottle base is arranged on a protection shell base of the preserving bottle, and preserving bottle silica gel fixing rings are arranged on the preserving bottle base; an integrated temperature measurement circuit board is arranged at the lower end of the preserving bottle base, a preserving bottle filter stand is arranged in the preserving bottle, and a preserving bottle filter net is arranged on the preserving bottle filter stand; and a preserving bottle top cover is arranged at the upper end of the preserving bottle, and a preserving bottle observing window is formed in the preserving bottle top cover. The biological specimen preserving bottle disclosed by the invention has the beneficial effects of being simple to operate, convenient to use, low in manufacturing cost and convenient to popularize, the time that specimens automatically dissolve and dry up is delayed, the normal shape of cells is maintained, and much convenience is brought for the work of inspection personnel.

The invention relates to the microbial medicine technology which belongs to the field of the biological medicine. A cyclosporine A material fermentation preparation process is provided. The process employs new culture medium components and concentrations and new culture condition for prolonging the synthesis period of the cyclosporine A producing strain and solving the foam problem in the process of big production.

The invention provides a method for preparing a non-fried instant freshwater fish, comprising the concrete steps of preparing a raw material fish, carrying out pretreatment (scale, head, tail and innards removing), washing clearly, cutting and shaping, soaking in ice water, soaking in water containing salt and vinegar, curing at a low temperature, draining, performing roast smoking, stoving, baking, spouting oil, filling, carrying out vacuum seal and microwave sterilization, boxing up to obtain the finished product. The fish prepared by adopting the method for preparing the non-fried instant freshwater fish has the advantages of no fishy smell, good taste, no poisonous or harmful substance of fried foods, little oil content and capability of guaranteeing the product quality.

The invention provides micro ecological additive and the application thereof in reducing ammonia emission. The additive comprises the following constituents: not less than 800 milligram / gram of citric acid, not less than 150 milligram / gram of phosphoric acid, and not less than 5*106 / gram lactobacillus. The additive takes citric acid and phosphoric acid as the main part, restrains the growth of putrefying bacteria in a setting tank, and slows down the autolysis of albumen in excreta, thereby achieving the effect that ammonia emission is completely eliminated. Compared with the existing additive, the micro ecological additive has the advantages of safety, high efficiency, specificity, low addition level and the like, the additive used in farms can achieve the purposes of reducing the ammonia emission in the farms, and mmonia zero emission in the farms is further achieved.

The invention aims to disclose a tea processing method to solve problems that ampelopsis grossedentata tea has poor taste, poor color and luster degree and too slow dissolution speed of effective components, and ensure that tea leaf fungus growing has good uniformity, and the tea is convenient for drinking, can keep the original quality of traditional fermented tea and is rich in organic selenium and flavonoids substances. The processing method successively comprises the following processing steps: preparing fresh ampelopsis grossedentata raw materials, carrying out fixation, carrying out pile fermentation, adding sodium selenite, steaming the tea, spraying eurotium cristatum spore powder, sterilizing after sealing tea leaves, carrying out tea leaf fungus growing, carrying out ultrasonic treatment, and baking and packaging. A large amount of eurotium cristatum spore powder is employed for rapid fermentation, thus miscellaneous bacteria growth is reduced, the tea leaf moldy problem cannot be caused, and the effect of a eurotium cristatum liquid culture medium on the original flavor of the tea leaves is avoided; with addition of the ampelopsis grossedentata leaves, the growth of harmful bacteria can be significantly inhibited, and the content of selenium in the tea leaves can be increased; and the ultrasonic treatment can reduce autolysis phenomenon of eurotium cristatum, can achieve the function of fixing eurotium cristatum, is conducive to furthest protect organic selenium, reduces high-temperature oxidation degradation of organic selenium, and simultaneously also can prevent eurotium cristatum from shrinking thalli at the high temperature.

The invention provides a technology for utilizing cyclocarya paliurus leaves and raw dark green tea for fermentation to make healthcare tea. A functionality tea product is obtained through the steps of pile fermentation, secondary fermentation, infrared ray fixing and the like. By means of the secondary fermentation mode, leaching speed of steam sterilization to cyclocarya paliurus leaf tea can be reduced, conversion of polysaccharide substances in the cyclocarya paliurus leaves is also effectively restrained, functionality ingredients of the cyclocarya paliurus leaves are also retained while the mouthfeel of tea soup is improved, reaction with tea polyphenols is avoided, uniform growth of golden flowers can be ensured, and quality of the composite tea is improved. A large amount of eurotium cristatum spore powder is adopted for carrying out fast short-time fermentation and long-time fermentation, and the problem that the cyclocarya paliurus leaf tea is green, astringent and bitter is solved thoroughly; meanwhile, growth of infectious microbes is reduced, mildewing of raw dark green tea is avoided, and the influence on original flavor of the tea from a culture medium in eurotium cristatum liquid is avoided.

The invention discloses a treatment method of a non-dry sea cucumber for preventing autolysis. The method comprises the following steps of boiling a frozen half-dry sea cucumber raw material in boiling water for 30-50 minutes, standing and cooling; removing intestines and teeth, cleaning, putting the raw material into an environment of 0-10 DEG C, changing the water every morning and evening and soaking for 3-4 days; boiling the soaked sea cucumber and spice water for 30-60 minutes; naturally cooling for later use; putting the sea cucumber and the spice water into a packaging bag at a certain ratio and sealing an opening; putting the packaged sea cucumber in the environment of 300-600MPa of pressure for 2-20 minutes and carrying out refrigerated preservation; and if normal-temperature preservation is needed, sterilizing the sea cucumber at 95-110 DEG C for 30-60 minutes. The sea cucumber product prepared by the method has the beneficial effects that (1) the problem that the sea cucumber cannot be seasoned or easily pickled in processing is solved, and the sea cucumber is directly fabricated into a seasoned product; (2) the autolysis phenomenon of the sea cucumber under the refrigeration or room temperature condition within an expiration date is retarded; and (3) the sea cucumber product is ready to enjoy when opened or can be used when opened, and is safe and convenient.

The invention relates to a health-care tea making process, in particular to a process method for making health-care tea by fermenting sweet tea leaves. The making process comprises the following steps: taking sweet tea raw materials, pile fermentation, tea steaming, eurotium cristatum spore powder spraying, sterilizing after tea leaves are sealed, tea leaves fungus growing, ultrasonic processing, drying and packing and the like. The sweet tea making process has the ultrasonic treatment capacity, can reduce the autolysis phenomenon of eurotium cristatum, also can achieve the function of fixing golden flowers, is beneficial to protection of organic selenium to the greatest degree, reduces the high-temperature oxidative degradation of organic selenium, and meanwhile, and can also prevent the thalli of the golden flowers from being reduced under the high temperature condition, the quick fermentation is carried out by adopting a large amount of eurotium cristatum spore powder, the growth of infectious microbes is slowed down, the problem of mildewing of the sweet tea leaves is not caused, the influence of a culture medium in the eurotium cristatum solution on the original flavor of the tea leaves is avoided, the content of tea polyphenol is reduced through fermentation, and the stomach irritation influence is reduced, and the sweet tea is easily suitable for being drunk by people with diabetes by means of the sweet taste of rubusoside.

In order to keep sea treasures fresh and alive, regulate the apoptosis rate of body coelom cells of sea cucumbers when they are stimulated by the outside world, and delay the autolysis of sea cucumber body walls, the present invention provides a method for delaying body wall autolysis of sea cucumbers, Inject the calcium ion chelating agent BAPTA-Na into the body cavity of the sea cucumber; the calcium ion chelating agent BAPTA-Na. The calcium ion chelating agent BAPTA-Na adopted in the present invention is poured into the body cavity of sea cucumbers in different doses within the semi-lethal concentration range. Dissolve, in order to achieve the purpose of keeping fresh and alive sea cucumber. BAPTA-Na is directly infused into the body cavity of fresh sea cucumber, which directly interacts with calcium ions in coelomocytes, and the in vivo experiment verifies that the new application of BAPTA-Na is convincing.

The invention provides a novel bio-fermentation method of streptomycin. According to the method, when a cell concentration is 50% v / v during the fermentation, the change of the kinetic conditions is stopped, dissolved oxygen is controlled by replenishing water, and CaCl2 is used in the later period of fermentation so that the potency of streptomycin is effectively improved by 50% or more. The method can keep the optimum dissolved oxygen concentration without change of the optimum medium concentration and ratio and without increasing of power load. Through use of CaCl2, the product potency is further improved in the later period of fermentation. The method has high operability and good stability, improves the overall potency by 50% to 60%, has high separation efficiency in the later extraction, and has the significant industrial application benefits.

The invention discloses a healthcare composite tea making technology. The technology comprises the following steps: deactivating enzymes of a fresh vine tea raw material, carrying out file fermentation, steaming, sprinkling with Eurotium cristatum spore powder, sealing tea leaves, sterilizing, fermenting the tea leaves, carrying out ultrasonic treatment, drying, and packaging. The technology effectively solves the problems of bad mouthfeel and color, too slow dissolve-out speed of active components of the vine tea, realizes ensure of the good tea leaf fermentation uniformity, convenient drinking, and maintenance of the original quality of a traditional fermented tea, and allows a tea rich in organic selenium and flavonoid substances to be obtained.

The invention provides healthcare berchemia floribunda tea product. The healthcare berchemia floribunda tea product comprises, by weight, 25-40 parts of berchemia floribunda tea, 10-20 parts of cyclocarya paliurus tea and 50 parts of fermented raw dark green tea. A method for manufacturing the healthcare berchemia floribunda tea product includes mixing the berchemia floribunda tea, the cyclocarya paliurus tea and the fermented raw dark green tea with one another to obtain mixtures, pressing the mixtures to obtain tea bricks and fermenting the tea bricks to obtain fermented mixtures; drying the fermented mixtures by means of infrared drying treatment until the moisture contents of the fermented mixtures are within the range of 12%. The healthcare berchemia floribunda tea product and the method have the advantages that the berchemia floribunda tea leaf and cyclocarya paliurus tea leaching speeds due to steam sterilization can be reduced by the aid of a secondary fermentation mode, conversion of polysaccharide substances in berchemia floribunda tea leaves and the cyclocarya paliurus tea can be effectively inhibited, functional ingredients in the berchemia floribunda tea leaves and the cyclocarya paliurus tea are reserved while taste of tea soup is improved, reaction with tea polyphenols can be prevented, uniform floating growth can be guaranteed, and the quality of the healthcare berchemia floribunda tea product which is compound tea can be improved.

The invention provides a technology for making health care tea by fermenting peristrophe baphica leaves and raw dark green tea, and discloses a peristrophe baphica tea product and a making method thereof. The making method includes the steps of pile fermentation, short-time fermentation long-time fermentation, infrared-ray fixation and the like, and the functional tea product is obtained. As two stage fermentation modes are adopted, the leaching speed of steam sterilization to active ingredients in peristrophe baphica tea can be decreased, conversion of polysaccharide substances in peristrophe baphica can be also effectively inhibited, the functional ingredients of the peristrophe baphica are saved while the taste of tea soup is improved, it is avoided that the functional ingredients and tea polyphenol are reacted, even growth of golden flowers can be also guaranteed, and the quality of the compound tea is improved. The autolysis phenomenon of eurotium cristatum can be reduced through infrared-ray drying treatment, and the effect of fixing the golden flowers can be also achieved; meanwhile, it can be also prevented that the golden flowers shrink thalluses at the high temperature, drying treatment is even and convenient through the high penetrating power, and the problems that the leaching speed of tea juice can be increased due to high-temperature fixation, or noise pollution and limitation of the size of treating substances exist during ultrasonic wave fixation are solved; a large amount of dihydromyricetin of the peristrophe baphica can be converted into soluble aroma substances, flavonoid substances of the peristrophe baphica tea are quite greatly used, and health-care function of the peristrophe baphica tea product is improved.

The invention discloses a method for improving escherichia coli protein expression quantity through staged dissolved oxygen control. Comprising the steps of strain activation, seed culture and fermentation tank fermentation, and the fermentation tank fermentation is divided into an initial culture stage, a fed-batch growth stage and an induction stage; wherein the induction stage is divided into first-stage induction and second-stage induction; the dissolved oxygen is controlled to be 25% or above through induction in the first stage, and the dissolved oxygen is controlled to be 0-10% through induction in the second stage. According to the method, induction is divided into two stages, and the culture conditions of each stage are controlled, so that high-density fermentation of the escherichia coli genetically engineered bacteria is realized. During the first-stage induction, a fermentation tank is in a maximum oxygen supply state, so that escherichia coli is in a high metabolism state, and rapid expression of protein is realized; during the second-stage induction, the oxygen supply state of the fermentation tank is reduced, so that the supplementing amount of the carbon source and the nitrogen source is reduced, the thalli are in a relatively low metabolism state, the aging and autolysis of the thalli can be slowed down, the vitality of the thalli can be maintained, and further expression of protein is realized.