Method for delaying autolyzing of body wall of stichopus japonicus

Abstract

In order to keep the precious marine products alive, the coelom cell apoptosis rate of the stichopus japonicus can be regulated and controlled when the stichopus japonicus is stimulated by the outside, and then autolyzing of the body wall of the stichopus japonicus can be delayed. The invention provides a method for delaying autolyzing of body wall of stichopus japonicas. A calcium ion chelating agent BAPTA-Na is injected into the coelom of the stichopus japonicus; the calcium ion chelating agent BAPTA-Na. The calcium ion chelating agent BAPTA-Na is injected into the coelom of the stichopus japonicus in different doses in a median lethal concentration range, and with the increase of the dosage, the coelom cell apoptosis of the stichopus japonicas is obviously inhibited, autolyzing of the body wall is delayed, and the purpose of preserving and keeping the stichopus japonicus is achieved. The BAPTA-Na is directly injected into the coelom of the living stichopus japonicus and can directlyact with calcium ions in a coelom cell, and the living experiment verifies that the new application of BAPTA-Na has persuasion.

Description

technical field [0001] The invention relates to the technical field of marine organisms, and more specifically relates to a method for inhibiting coelomocyte apoptosis of sea cucumbers and delaying body wall autolysis of sea cucumbers by applying calcium ion chelating agent BAPTA-Na. Background technique [0002] Calcium ion chelating agent BAPTA-Na chemical name is 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid disodium salt, molecular formula is C 22 h 22 N 2 Na 2 o 10 , with a molecular weight of 520.40, is a commercially synthesized calcium ion chelating agent. According to the existing literature search, BAPTA has the following effects: 1. It can weaken the neurotransmitter central mammalian synapse release and reduce neuron ischemia in vivo; 2. It can significantly block the EGF-induced MCF-7 breast cancer cells Migration; 3. BAPTA pretreatment of pulmonary microvascular endothelial cells can promote the recovery of PMVECs mitochondrial permeability tran...

AI Technical Summary

Problems solved by technology

This type of method has a higher cost, a shorter preservation time, and is not suitable for long-distance transportation; there is also an anesthesia preservation, using MS-222, 3-aminobenzoic acid ethyl ester methanesulfonate, but its solution is acidic, and MS-222 cannot reduce CO when seafood is anesthetized and kept alive 2 Discharge, it must be raised in clean water for 21 days before it can be sold, and its operation is relatively cumbersome

At present, it is difficult to keep the sea cucumber fresh and alive in seafood, because it is stimulated by the outside world, and it will autolyze

Images