Method for delaying autolyzing of body wall of stichopus japonicus

A technology of autolysis and ginseng, applied in fish farming, application, animal husbandry and other directions, can solve the problems of short survival time, complicated operation, autolysis, etc., to delay the autolysis of body wall and inhibit cell apoptosis. Effect

What is AI technical title?
AI technical title is built by PatSnap AI team. It summarizes the technical point description of the patent document.
A technology of autolysis and ginseng, applied in fish farming, application, animal husbandry and other directions, can solve the problems of short survival time, complicated operation, autolysis, etc., to delay the autolysis of body wall and inhibit cell apoptosis. Effect

CN107873587AActive Publication Date: 2018-04-06DALIAN POLYTECHNIC UNIVERSITY

2 Cites 4 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.

Viewing Examples

Smart Image

Examples

Experimental program

Comparison scheme

Effect test

Embodiment approach

[0018] Detection of BAPTA-Na half-lethal concentration: take body cavity fluid near 1 / 3 of the anus of sea cucumber, mix equal volumes of body cavity fluid of 9 sea cucumbers, filter with 300-600 mesh filter cloth, take 100 μL of fresh body cavity fluid in 96 In the well plate; add 0.00-2.00mM calcium ion chelator BAPTA-Na with different gradients; add 10μL MTT solution to each well, and incubate at room temperature for 3-5h; add 100μL Formanzan solution to each well, and incubate at room temperature in the dark 3 to 5 hours; use a multi-functional microplate reader to measure the absorbance at 570nm; calculate the IC based on the cell viability 50 It is 1.47mM, so select high, medium and low doses below 1.47mM to pour into the body cavity of sea cucumber.

[0019] Experimental grouping: Fresh and alive sea cucumbers were randomly divided into four groups, with 9 sea cucumbers in each group. UVA group Apostichopus was treated by ultraviolet light (UVA, 10~20W / m 2 ) for 30-60...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

PUM

Login to View More

Abstract

In order to keep the precious marine products alive, the coelom cell apoptosis rate of the stichopus japonicus can be regulated and controlled when the stichopus japonicus is stimulated by the outside, and then autolyzing of the body wall of the stichopus japonicus can be delayed. The invention provides a method for delaying autolyzing of body wall of stichopus japonicas. A calcium ion chelating agent BAPTA-Na is injected into the coelom of the stichopus japonicus; the calcium ion chelating agent BAPTA-Na. The calcium ion chelating agent BAPTA-Na is injected into the coelom of the stichopus japonicus in different doses in a median lethal concentration range, and with the increase of the dosage, the coelom cell apoptosis of the stichopus japonicas is obviously inhibited, autolyzing of the body wall is delayed, and the purpose of preserving and keeping the stichopus japonicus is achieved. The BAPTA-Na is directly injected into the coelom of the living stichopus japonicus and can directlyact with calcium ions in a coelom cell, and the living experiment verifies that the new application of BAPTA-Na has persuasion.

Description

technical field [0001] The invention relates to the technical field of marine organisms, and more specifically relates to a method for inhibiting coelomocyte apoptosis of sea cucumbers and delaying body wall autolysis of sea cucumbers by applying calcium ion chelating agent BAPTA-Na. Background technique [0002] Calcium ion chelating agent BAPTA-Na chemical name is 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid disodium salt, molecular formula is C 22 h 22 N 2 Na 2 o 10 , with a molecular weight of 520.40, is a commercially synthesized calcium ion chelating agent. According to the existing literature search, BAPTA has the following effects: 1. It can weaken the neurotransmitter central mammalian synapse release and reduce neuron ischemia in vivo; 2. It can significantly block the EGF-induced MCF-7 breast cancer cells Migration; 3. BAPTA pretreatment of pulmonary microvascular endothelial cells can promote the recovery of PMVECs mitochondrial permeability tran...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

Application Information

Patent Timeline

06 Apr 2018

Publication

CN107873587A

IPC: A01K61/30

CPC: A01K61/30

Inventors: 宋亮; 周大勇