The invention provides a stabilizing and buffering liquid of low molecular weight sugars used for lyophilization preservation of platelet, which is water solution with 35-45mM thickness by adding thelow molecular weight sugars on the basis of basic buffering liquid composing prescriptions (GS), wherein the basic buffering liquid composing prescriptions (GS) comprises 140mMNaCL, 5Mmkcl,12mM sodiumcitrate, 10mM glucose, 12.5mM sucrose and 1mug / mLPGE1. The low molecular weight sugars are trehalose. After the platelet is stabilized and protected by the stabilizing and buffering liquid of low molecular weight sugars of the invention, the platelet is freeze-dried with rehydration. The recovery ratio of a cell is above 70% and accumulating activity evoked by ADP can achieve 50% of fresh platelet. The platelet freeze-dried with rehydration has normal shape and complete structure, thereby a new solution is provided for freeze-drying conservation of the platelet.