Preparation method of corn protein nano-particles

A technology of zein and nanoparticles, which is applied in the field of preparation of zein nanoparticles, can solve problems such as adverse reactions and reduce the frequency of medication, and achieve the effects of reducing drug side effects, increasing residence time, and narrow distribution

Inactive Publication Date: 2010-07-28
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be used as a drug carrier for humans and animals to solve the problem of adverse reactions in vivo caused by excessive local concentrations of some drugs. Through the degradation of the carrier in the body, the drug gradually diffuses to achieve a sustained release effect, thereby reducing the frequency of medication. Reduce the peak and valley phenomenon of blood concentration, improve efficacy and safety

Method used

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  • Preparation method of corn protein nano-particles
  • Preparation method of corn protein nano-particles
  • Preparation method of corn protein nano-particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 300 mg of zein was dissolved in 15 ml of a mixed solvent of acetone and dimethyl sulfoxide at a volume ratio of 7:3 to prepare a 20 mg / ml zein solution. Pump carbon dioxide into the autoclave. After the autoclave reaches the preset temperature of 40°C and pressure of 10MPa and stabilizes for 5 minutes, pump the zein solution at a flow rate of 1.0ml / min. After the solution is pumped, continue to feed carbon dioxide for 30 minutes, stop feeding carbon dioxide, release the pressure, and collect the particles in the reaction kettle to obtain zein nanoparticles.

[0026] The morphology of the prepared zein nanoparticles was observed with a field emission scanning electron microscope. Stick the solid powder directly on the conductive glue and spray gold under vacuum state to observe. The result is as figure 1 shown.

[0027] The prepared zein nanoparticles were tested for particle size and distribution with a nanoparticle size analyzer, and the sample to be tested was disp...

Embodiment 2

[0029] 150 mg of zein was dissolved in 15 ml of mixed solvent of acetone and dimethyl sulfoxide at a volume ratio of 7:3 to prepare a 10 mg / ml zein solution. Pump carbon dioxide into the autoclave. After the autoclave reaches the preset temperature of 40°C and pressure of 10MPa and stabilizes for 5 minutes, pump the zein solution at a flow rate of 1.0ml / min. After the solution is pumped, continue to feed carbon dioxide for 30 minutes, stop feeding carbon dioxide, release the pressure, and collect the particles in the reaction kettle to obtain zein nanoparticles.

[0030] The particle size and distribution detection of zein nanoparticles are the same as in Example 1, the particle size distribution is between 110nm-300nm, and the average particle size is 217nm.

Embodiment 3

[0032] 300 mg of zein was dissolved in 15 ml of a mixed solvent of acetone and dimethyl sulfoxide at a volume ratio of 7:3 to prepare a 20 mg / ml zein solution. Pump carbon dioxide into the autoclave. After the autoclave reaches the preset temperature of 32°C and pressure of 10MPa and stabilizes for 5 minutes, pump the zein solution at a flow rate of 1.0ml / min. After the solution is pumped, continue to feed carbon dioxide for 30 minutes, stop feeding carbon dioxide, release the pressure, and collect the particles in the reaction kettle to obtain zein nanoparticles.

[0033] The particle size and distribution detection of zein nanoparticles are the same as in Example 1, the particle size distribution is between 105nm-280nm, and the average particle size is 197nm.

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Abstract

The invention discloses a preparation method of corn protein nano-particles in the technical field of medicine preparation, comprising: adding zein to mixed solvent to prepare corn protein solution; pumping CO2 into a high-pressure reactor, and pumping the corn protein solution into the high-pressure reactor after the reactor gets the reaction pressure and the reaction temperature; adding CO2 to the high-pressure reactor further until the end of the drying of the residual solvent, then obtaining the corn protein nano-particles. For the corn protein nano-particles prepared in the invention, the particle has round and normal shape, uniform size, and 100-400 nm of diameter.

Description

technical field [0001] The invention relates to a preparation method in the technical fields of medicine and food, in particular to a preparation method of zein nanoparticles. Background technique [0002] The research on the use of microspheres as drug carriers began in the mid-1970s. Because of its targeting to specific organs and tissues and the sustained release of drugs, it has become a hot spot in the study of sustained and controlled release dosage forms. Microspheres can be used for injection (intravenous injection, intramuscular injection), oral administration, nasal drop, subcutaneous implantation or joint cavity administration. The natural polymer drug sustained-release system has the characteristics of general drug sustained release, such as: adjusting and controlling the release rate of drugs to achieve long-acting purposes; reducing the number of administration and drug stimulation, reducing toxic and side effects, and improving efficacy; increasing drug stabil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42
Inventor 林长春赵亚平夏菲洪流孙丽君
Owner SHANGHAI JIAO TONG UNIV
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