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Cell stent with carbon nano tube and preparation method thereof

A cell scaffold and carbon nanotube technology, applied in the field of tissue engineering, can solve the problems of single uncontrollable mechanical properties, unsatisfactory, limited application scope of pure PLGA bioscaffold materials, etc., and achieve excellent mechanical properties, biological properties, and excellent mechanical properties. Effect

Active Publication Date: 2014-06-25
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current pure PLGA scaffold material has defects. For example, the tissue engineered tissue it constructs often has the disadvantage of single and uncontrollable mechanical properties, and cannot be widely used in tissue engineered bone, tissue engineered skin, tissue engineered muscle, etc. Second, it cannot meet the electrical performance requirements such as bioelectric current conduction between synaptic links when constructing tissue-engineered peripheral nerve tissue, which limits the application range of pure PLGA bio-scaffold materials

Method used

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  • Cell stent with carbon nano tube and preparation method thereof
  • Cell stent with carbon nano tube and preparation method thereof
  • Cell stent with carbon nano tube and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Preparation of samples to be tested: PLGA15% (W / V) + CTNs0.2% (W / V)

[0034] The preparation stage of the electrospinning solution, that is, using HFIP with a wide dissolution spectrum as the solvent system for carrying different polymers to co-exist, and preparing PLGA and CNTs into hexafluoroethylene propanol polymer solutions and suspensions respectively, Then combine the two, magnetic stirring and mixing to obtain a spinning solution with uniform composition.

[0035] 1. First, weigh 1.05g of PLGA solid particles as the main body of electrospinning polymers and dissolve them in 3ml HFIP (half-volume total solvent volume), heat in a water bath (37°C) for 4-5 hours until completely dissolved, and obtain PLGA Solution, that is, PLGA polymer spinning liquid matrix.

[0036] 2. Then add 0.014g of CNTs into 4ml HFIP (half-volume total solvent volume) for ultrasonic treatment for 2 hours to form a uniformly dispersed CNTs suspension. After stirring with a magnetic sti...

Embodiment 2

[0042] Embodiment 2 Surface morphology and diameter distribution

[0043] Cut the sample materials of the test group and the control group: PLGA15% (W / V) + CTNs0.2% (W / V) and the simple component PLGA15% (W / V) to cut out 1cm×1cm square samples, spray gold half Hours, the microscopic morphology was examined under a scanning electron microscope. After spraying gold and irradiating the scanning electron microscope, select three 1000× magnified field of view photos for each material, so that the field of view should reflect the fiber microscopic distribution of the selected material as representatively as possible. Six fields of view were randomly selected from each scanning electron microscope photo, and 5 fibers were randomly selected in each field of view to measure the diameter. A total of 90 fibers were selected for each concentration of CNTs material, and the diameters of these fibers were measured and analyzed using imageJ software.

[0044] Results and analysis: The above...

Embodiment 3

[0045] Embodiment 3 mechanical performance detection

[0046] The Young's modulus, yield strength, and fracture strain of the material were obtained through the tensile failure test of the INSTRON5948 mechanical tensile tester. All material samples have been pretreated as follows before the tensile test: the sample materials of the test group and the control group: PLGA15% (W / V) + CTNs0.2% (W / V) and simple component PLGA15% (W / V) were cut into 5cm×1cm dumbbell-shaped structures, and the thickness of each material was measured with a spiral micrometer. A 1cm×1cm square clamping position is reserved at each end, and the working area is 3cm×1cm, and 5 groups of parallel experiments are done for each material.

[0047] Tensile failure test: Clamp both ends of the material strip on the hydraulic clamp, and pull the material axially at both ends at a constant speed of 10mm / min after fixing until the material breaks. The stress-strain curve of each material is obtained, and the Yo...

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Abstract

The invention discloses a cell stent with a carbon nano tube and a preparation method thereof. PLGA (PolyLactic-co-Glycolic Acid) and the carbon nano tube are mixed in a certain ratio to improve agglomeration of the carbon nano tube. The PLGA solution added with the carbon nano tube is prepared into a micro-nano-scale three-dimensional cell stent through an electrospinning technique. For the PLGA stent with the carbon nano tube, the mechanical property and the hydrophilcity are remarkably improved compared with those of pure PLGA stent. Cell experiments show that cells have good adhesion and grow vigorously on a material with the carbon nano tube while cells on the pure PLGA stent are easy to fall and cannot fully maintain the normal shape. Therefore, the PLGA cell stent with the carbon nano tube has a wide application prospect in the tissue engineering field, particularly nervous tissue, heart tissue and bone tissue engineering stents.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and relates to a cell scaffold containing carbon nanotubes and a preparation method thereof, in particular to a polylactic acid-glycolic acid copolymer three-dimensional cell scaffold containing carbon nanotubes and a preparation method thereof. Background technique [0002] Tissue engineering can achieve tissue regeneration. Tissue-engineered scaffolds can provide the foundation needed to grow cells damaged by disease, injury, birth defects, and more. The scaffold material must have properties such as biocompatibility, biodegradability, high porosity, and mechanical adaptability. Electrospinning technology can prepare three-dimensional porous fibers with the same structure as natural extracellular matrix. This kind of fiber membrane can be used as an ideal tissue engineering scaffold to facilitate the adhesion, proliferation and migration of target cells. [0003] With the deepening...

Claims

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Application Information

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IPC IPC(8): A61L27/08A61L27/18D04H1/728
Inventor 刘晶徐英辉冷志前苗辉吕正钧
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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