Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Small molecular sugar stable buffer used for blood platelet frozen-dried preservation

A small molecule sugar and buffer technology, which can be used in blood diseases, freeze-dried transportation, preservation of human or animal bodies, etc., can solve the problem of low aggregation activity, and achieve the effect of complete structure and normal shape.

Inactive Publication Date: 2008-04-30
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
View PDF0 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, Brry SJ. et al. used glucose to incubate platelets and then freeze-dried and rehydrated, and obtained a cell recovery rate of 75%-85%, but the aggregation activity was low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Small molecular sugar stable buffer used for blood platelet frozen-dried preservation
  • Small molecular sugar stable buffer used for blood platelet frozen-dried preservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] Trehalose was added to the basic buffer GS formula to prepare the load solution of GS (T35), which was a stable aqueous buffer solution containing 35mM trehalose, and its osmotic pressure was 339±8mOMS. The aqueous solution contains: trehalose 35mM, glucose 10mM, sucrose 12.5mM, sodium citrate 12mM, KCL 5mM, NaCL 140mM, PGE 1μg / mL.

[0042] According to the aforementioned 2) small molecule sugar loading experiment and 3) detection of various indicators of platelets loaded with small molecule sugar, the results:

[0043] ① Cell recovery rate: greater than 70%;

[0044] ② Aggregation activity: greater than 60%;

[0045] ③Morphological structure: The morphological structure is basically normal, see Figure 2b.

[0046] According to the aforementioned 4) the platelets were lyophilized and hydrated and the platelet samples obtained after 5) were freeze-dried and hydrated for physiological index detection, the results:

[0047] ① Cell recovery rate: greater than 70%;

[00...

experiment example

[0054] According to the recipe in Table 1, trehalose was added to the basic buffer to prepare loading solutions such as stable buffers GS (T30), GS (T40), and GS (T45). For example: GS (T40) stabilization buffer contains 40mM trehalose, and its osmotic pressure is 386±6mOMS.

[0055] At the same time, GS was used as a comparison sample in the experiment. Another trehalose solution (10 mM KCl, 10 mM EGTA, 10 mM imidazole, 1 μM PGE1, 100 mM NaCl, 40 mM trehalose) prepared by Wolkers WF. et al. was used as a control sample.

[0056] Table 1: Stabilization buffers containing different concentrations of trehalose

[0057] GS(T30)

GS(T40)

GS(T45)

GS

Trehalose (mM)

Glucose (mM)

Sucrose (mM)

Sodium citrate (mM)

KCL(mM)

NaCl(mM)

PGE (μg / mL)

30

10

12.5

12

5

140

1

40

10

12.5

12

5

140

1

45

10

12.5

12

5

140

1

0

10

12.5

12 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a stabilizing and buffering liquid of low molecular weight sugars used for lyophilization preservation of platelet, which is water solution with 35-45mM thickness by adding thelow molecular weight sugars on the basis of basic buffering liquid composing prescriptions (GS), wherein the basic buffering liquid composing prescriptions (GS) comprises 140mMNaCL, 5Mmkcl,12mM sodiumcitrate, 10mM glucose, 12.5mM sucrose and 1mug / mLPGE1. The low molecular weight sugars are trehalose. After the platelet is stabilized and protected by the stabilizing and buffering liquid of low molecular weight sugars of the invention, the platelet is freeze-dried with rehydration. The recovery ratio of a cell is above 70% and accumulating activity evoked by ADP can achieve 50% of fresh platelet. The platelet freeze-dried with rehydration has normal shape and complete structure, thereby a new solution is provided for freeze-drying conservation of the platelet.

Description

technical field [0001] The invention belongs to the field of freeze-drying preservation of platelets, and relates to a small molecule sugar stabilizing buffer. Background technique [0002] The study of freeze-dried platelet preservation is of great significance. Firstly, freeze-dried platelets have broad application prospects in treating the wounded and sick in hospitals. The demand for platelets in my country's direct market value is estimated to be about 1 billion per year (50-700,000 bags × 1700 Yuan), the indirect benefits cannot be estimated. And it is suitable for storage and mutual adjustment in various large, medium and small hospitals in different regions and remote island areas. Secondly, freeze-dried platelets have important military value. Soldiers can carry them with them for field rescue, save the lives of the wounded, and play a role in maintaining the combat effectiveness of troops. Thirdly, it can effectively prevent the spread of transfusion-transmitted d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/14A61K47/26A61K9/19C12N5/08A61P7/00G01N33/50G01N33/53A01N1/02A61K35/19
Inventor 王捷熙韩颖杨超王艳刘敏霞权国波刘安
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com