Tissue culture and rapid propagation method of caladium bicolor suitable for industrialized production

A technology for tissue culture, rapid propagation and coleus taro, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as few systematic studies

Active Publication Date: 2021-04-27
ZHANJIANG SUGARCANE RES CENT GUANGZHOU SUGARCANE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few systematic studies on the tissue culture and ...

Method used

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  • Tissue culture and rapid propagation method of caladium bicolor suitable for industrialized production
  • Tissue culture and rapid propagation method of caladium bicolor suitable for industrialized production
  • Tissue culture and rapid propagation method of caladium bicolor suitable for industrialized production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Effects of Different Induction Mediums on the Callus Induction of Coleus variegata

[0043] Collect the unexpanded roll stick-shaped taro coleus leaves, wipe them with 75% alcohol cotton, and put them into clean bags. Then disinfect it with 0.1% mercuric chloride on the ultra-clean workbench for 7 minutes, and rinse it with sterile water for 4 times. Cut into 1 mm slices and inoculate induction medium (see Table 1 for the specific formulation of induction medium) for callus induction culture. Shade with a black cloth and cultivate in complete darkness.

[0044] The induction medium is composed of basal medium and hormone combination, and the basal medium includes the following components: MS medium, 30 g / L sucrose, 7 g / L carrageenan, and pH 5.8.

[0045] 10 young leaf slices were inoculated for each treatment, and the experiment was repeated 3 times. According to the growth situation, the healing time and healing rate were counted.

[0046] Average callus ...

Embodiment 2

[0050] The influence of embodiment 2 different bud-inducing mediums on the induction culture of Coleus variegata buds

[0051] The callus induced in Example 1 was inoculated into the shoot induction medium (see Table 2 for the specific formulation of the shoot induction medium) for shoot induction culture. The light intensity for cultivation is 2000 lux, and the light time is 12 hours / day.

[0052] The bud induction medium is composed of basal medium and hormone combination, and the basal medium includes the following components: MS medium, 30 g / L of sucrose, 7 g / L of carrageenan, and pH 5.8.

[0053] Each treatment was inoculated with 20 shoot segments, and the experiment was repeated 3 times. According to the growth situation, the corresponding embryoid body and cluster bud induction time, induction rate and bud body state were counted.

[0054] Table 2 Effects of different hormone combinations on the induction of taro coleus buds and embryoid bodies

[0055]

[0056] ...

Embodiment 3

[0057] The influence of embodiment 3 different propagation mediums on the multiplication and cultivation of Coleus variegata

[0058] The cluster buds and embryoid bodies induced in Example 2 were inoculated into the proliferation medium (see Table 3 for the specific formulation of the proliferation medium) for proliferation culture. The light intensity for cultivation is 1500-2000 lux, and the light time is 12 hours / day.

[0059] The proliferation medium is composed of basal medium and hormone combination, and the basal medium includes the following components: MS medium, 30 g / L sucrose, 7 g / L carrageenan, and pH 5.8.

[0060] The data at this stage are the average rooting and multiplication coefficient and seedling growth potential derived from statistics of corresponding data during experiments and mass production.

[0061] Table 3 Effects of different hormone combinations on the proliferation of taro coleus buds and embryoid bodies

[0062]

[0063]

[0064] It can...

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Abstract

The invention belongs to the technical field of tissue culture, and relates to a tissue culture and rapid propagation research method suitable for industrialized production. The method is summarized and concluded on the basis of experimental research and production for two years on the basis of a new variety of caladium bicolor introduced by Thai, has strong operability and can be completely used for guiding production. According to the invention, the complete rapid induction, multiplication, rooting and seedling hardening propagation method which has high inductivity and propagation multiple and is suitable for industrial production is established for the first time, a batch rapid propagation situation is formed, and the propagation generations, the hormone concentration of a culture medium and the proportion are controlled to change along with the change of the generations, stability, activity and the proliferation coefficient of seedlings are ensured, and the yield and quality are improved; and according to the method, a production manner of simultaneously propagating and rooting is adopted, so that the production cost is reduced, the production progress is accelerated, the propagation coefficient is ensured, the quality of rooted seedlings is ensured, the method is completely suitable for large-scale industrialized production, and the breeding efficiency of the caladium bicolor is greatly improved.

Description

technical field [0001] The invention relates to the technical field of tissue culture, and more particularly relates to a method for tissue culture and rapid propagation of taro calyx that is suitable for industrial production. Background technique [0002] Caladuim bicolor, also known as Caladuim bicolor, belongs to the genus Caladuim bicolor, and is native to South America. The habit of growing well in light is currently a popular indoor foliage plant. [0003] At present, Coleus calyx mainly relies on tuber ramets for reproduction, but tuber reproduction is limited. A good tuber can produce 3 to 4 seedlings at most once a year, and the reproduction rate is low and the growth is slow. Commercial reproduction can also be through seeds, but seed reproduction is difficult because the seeds are very small and the mortality rate is high. The cost of plants obtained through seed growth and reproduction is too high, and the offspring of seed reproduction are prone to variation a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 官锦燕谭嘉娜罗青文罗剑飘文明富许玉婵黄海英
Owner ZHANJIANG SUGARCANE RES CENT GUANGZHOU SUGARCANE RES INST
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