Tissue culture and rapid propagation method of caladium bicolor suitable for industrialized production
A technology for tissue culture, rapid propagation and coleus taro, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as few systematic studies
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Embodiment 1
[0042] Example 1 Effects of Different Induction Mediums on the Callus Induction of Coleus variegata
[0043] Collect the unexpanded roll stick-shaped taro coleus leaves, wipe them with 75% alcohol cotton, and put them into clean bags. Then disinfect it with 0.1% mercuric chloride on the ultra-clean workbench for 7 minutes, and rinse it with sterile water for 4 times. Cut into 1 mm slices and inoculate induction medium (see Table 1 for the specific formulation of induction medium) for callus induction culture. Shade with a black cloth and cultivate in complete darkness.
[0044] The induction medium is composed of basal medium and hormone combination, and the basal medium includes the following components: MS medium, 30 g / L sucrose, 7 g / L carrageenan, and pH 5.8.
[0045] 10 young leaf slices were inoculated for each treatment, and the experiment was repeated 3 times. According to the growth situation, the healing time and healing rate were counted.
[0046] Average callus ...
Embodiment 2
[0050] The influence of embodiment 2 different bud-inducing mediums on the induction culture of Coleus variegata buds
[0051] The callus induced in Example 1 was inoculated into the shoot induction medium (see Table 2 for the specific formulation of the shoot induction medium) for shoot induction culture. The light intensity for cultivation is 2000 lux, and the light time is 12 hours / day.
[0052] The bud induction medium is composed of basal medium and hormone combination, and the basal medium includes the following components: MS medium, 30 g / L of sucrose, 7 g / L of carrageenan, and pH 5.8.
[0053] Each treatment was inoculated with 20 shoot segments, and the experiment was repeated 3 times. According to the growth situation, the corresponding embryoid body and cluster bud induction time, induction rate and bud body state were counted.
[0054] Table 2 Effects of different hormone combinations on the induction of taro coleus buds and embryoid bodies
[0055]
[0056] ...
Embodiment 3
[0057] The influence of embodiment 3 different propagation mediums on the multiplication and cultivation of Coleus variegata
[0058] The cluster buds and embryoid bodies induced in Example 2 were inoculated into the proliferation medium (see Table 3 for the specific formulation of the proliferation medium) for proliferation culture. The light intensity for cultivation is 1500-2000 lux, and the light time is 12 hours / day.
[0059] The proliferation medium is composed of basal medium and hormone combination, and the basal medium includes the following components: MS medium, 30 g / L sucrose, 7 g / L carrageenan, and pH 5.8.
[0060] The data at this stage are the average rooting and multiplication coefficient and seedling growth potential derived from statistics of corresponding data during experiments and mass production.
[0061] Table 3 Effects of different hormone combinations on the proliferation of taro coleus buds and embryoid bodies
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[0064] It can...
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